(Circular) (Six-base) MAPSORT of: pFN21AB7926.seq Check: 3967 from: 1 to: 5755 pFN21AB7926 5755 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2206 3065 3818 5202 5470 1632 Size: 574 859 753 1384 268 1917 Fragments arranged by size: 1917 1384 859 753 574 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 2863 4654 5631 1203 Size: 1660 1791 977 1327 Fragments arranged by size: 1791 1660 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5755 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 3498 5070 3498 Size: 1572 4183 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 2873 3064 3290 3442 5551 1493 Size: 1380 191 226 152 2109 1697 Fragments arranged by size: 2109 1697 1380 226 191 152 AseI AT'TA_AT Cuts at: 160 1032 2373 4309 160 Size: 872 1341 1936 1606 Fragments arranged by size: 1936 1606 1341 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2140 3076 4531 618 Size: 325 217 627 353 936 1455 1842 Fragments arranged by size: 1842 1455 936 627 353 325 217 BanII G_rGCy'C Cuts at: 1391 2164 3074 1391 Size: 773 910 4072 Fragments arranged by size: 4072 910 773 BbeI G_GCGC'C Cuts at: 2144 2144 Size: 5755 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2447 928 Size: 971 548 4236 Fragments arranged by size: 4236 971 548 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2045 2079 3946 3980 1903 Size: 34 108 34 1867 34 3678 Fragments arranged by size: 3678 1867 108 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 2601 2932 1548 Size: 1053 331 4371 Fragments arranged by size: 4371 1053 331 BfrBI ATG'CAT Cuts at: 2635 2635 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2711 5750 2711 Size: 3039 2716 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 3816 4972 1790 Size: 2026 1156 2573 Fragments arranged by size: 2573 2026 1156 BmgBI CAC'GTC Cuts at: 1318 2975 1318 Size: 1657 4098 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2294 2348 2795 4415 1239 Size: 977 78 54 447 1620 2579 Fragments arranged by size: 2579 1620 977 447 78 54 Bpu10I CC'TnA_GC Cuts at: 2190 2190 Size: 5755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3880 4745 5043 3880 Size: 865 298 4592 Fragments arranged by size: 4592 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3546 493 Size: 711 2342 2702 Fragments arranged by size: 2702 2342 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2141 3562 3944 4579 Size: 53 83 186 936 608 1421 382 635 Cuts at: 4579 275 Size: 1451 Fragments arranged by size: 1451 1421 936 635 608 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2146 2176 1178 Size: 30 165 30 743 30 4757 Fragments arranged by size: 4757 743 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2264 2282 2291 2303 2306 2309 2312 Size: 958 120 18 9 12 3 3 3 Cuts at: 2312 2372 2375 3030 1186 Size: 60 3 655 3911 Fragments arranged by size: 3911 958 655 120 60 18 12 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 4958 5454 4958 Size: 496 5259 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2097 1163 Size: 573 361 4821 Fragments arranged by size: 4821 573 361 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3816 3901 4972 3816 Size: 85 1071 4599 Fragments arranged by size: 4599 1071 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 BsmBI CGTCTCn'nnnn_ Cuts at: 2147 2147 Size: 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2164 3074 3816 3901 4972 1391 Size: 399 374 910 742 85 1071 2174 Fragments arranged by size: 2174 1071 910 742 399 374 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2683 3110 844 Size: 1839 427 3489 Fragments arranged by size: 3489 1839 427 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3000 4250 4424 65 Size: 1183 1752 1250 174 1396 Fragments arranged by size: 1752 1396 1250 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2137 2337 4399 4586 1700 Size: 110 327 200 2062 187 2869 Fragments arranged by size: 2869 2062 327 200 187 110 BsrGI T'GTAC_A Cuts at: 96 2589 96 Size: 2493 3262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstYI r'GATC_y Cuts at: 1972 2711 3085 3452 3837 3854 5306 5392 5404 Size: 739 374 367 385 17 1452 86 12 Cuts at: 5404 5750 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 739 385 374 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4264 Fragments arranged by size: 4264 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 3271 4065 4085 3271 Size: 794 20 4941 Fragments arranged by size: 4941 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3202 4091 5720 Size: 54 1425 50 275 18 1372 889 1629 Cuts at: 5720 8 Size: 43 Fragments arranged by size: 1629 1425 1372 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 2280 3685 1353 Size: 906 21 1405 3423 Fragments arranged by size: 3423 1405 906 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2070 4342 4716 4862 387 Size: 1064 138 481 2272 374 146 1280 Fragments arranged by size: 2272 1280 1064 481 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2216 2294 2348 2795 3065 3818 Size: 393 574 10 78 54 447 270 753 Cuts at: 3818 4415 5202 5470 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 753 597 574 447 393 270 268 78 54 10 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2046 2144 2368 4902 1678 Size: 368 98 224 2534 2531 Fragments arranged by size: 2534 2531 368 224 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2146 2178 2530 2562 4396 Size: 32 135 32 574 32 352 32 1834 Cuts at: 4396 4428 4470 4502 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1834 574 352 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KasI G'GCGC_C Cuts at: 2140 2140 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2235 2544 2922 2995 4869 5053 1305 Size: 930 309 378 73 1874 184 2007 Fragments arranged by size: 2007 1874 930 378 309 184 73 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3933 Fragments arranged by size: 3933 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3713 4072 4231 518 Size: 648 2547 359 159 2042 Fragments arranged by size: 2547 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2123 3171 3848 4996 5241 1606 Size: 517 1048 677 1148 245 2120 Fragments arranged by size: 2120 1148 1048 677 517 245 NaeI GCC'GGC Cuts at: 2339 2339 Size: 5755 NarI GG'CG_CC Cuts at: 2141 2141 Size: 5755 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4264 Fragments arranged by size: 4264 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NgoMIV G'CCGG_C Cuts at: 2337 2337 Size: 5755 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NsiI A_TGCA'T Cuts at: 2637 2637 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2867 3113 4658 1132 Size: 97 1638 246 1545 2229 Fragments arranged by size: 2229 1638 1545 246 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 2863 4654 2863 Size: 1791 3964 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5755 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2307 1613 Size: 694 5061 PsiI TTA'TAA Cuts at: 2553 3325 2553 Size: 772 4983 PstI C_TGCA'G Cuts at: 830 2068 3107 830 Size: 1238 1039 3478 Fragments arranged by size: 3478 1238 1039 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2064 2497 3103 4238 4919 5110 826 Size: 219 1019 433 606 1135 681 191 1471 Fragments arranged by size: 1471 1135 1019 681 606 433 219 191 SfoI GGC'GCC Cuts at: 2142 2142 Size: 5755 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3859 4760 5022 820 Size: 197 926 1916 901 262 1553 Fragments arranged by size: 1916 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2004 3194 513 Size: 552 939 1190 3074 Fragments arranged by size: 3074 1190 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2583 3782 3799 3952 4137 4582 325 Size: 1255 1003 1199 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1199 1003 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2530 2589 4001 Size: 274 80 33 51 1443 553 59 1412 Cuts at: 4001 5713 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1412 553 274 138 80 59 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2676 2708 5715 5747 2676 Size: 32 3007 32 2684 Fragments arranged by size: 3007 2684 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 2608 2659 3091 2608 Size: 51 432 5272 Fragments arranged by size: 5272 432 51 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 2454 3884 2454 Size: 1430 4325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI