Restriction map for
pFN21AE0040
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspHI AviII AvrII
BamHI BbeI BbsI BbvCI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaHI BseAI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
ClaI CpoI DrdI Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 255 GTAC GT^AC
AflIII 1817 ACATGT A^C[GA][CT]GT
AgeI 1545 ACCGGT A^CCGGT
AhdI 1875 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1415 CCTCAGG CC^T[ACGT]AGG
Asp700I 579 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1875 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 805 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1666 CACGTG CAC^GTG
BcgI 1835 GCAGGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1006 GTATCC GTATCC(6/5)
BglI 1606 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1666 CACGTG [CT]AC^GT[GA]
BsaBI 1140 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 511 GGTCTC GGTCTC(1/5)
BsaWI 1545 ACCGGT [AT]^CCGG[AT]
BspLU11I 1817 ACATGT A^CATGT
BsrI 1876 ACTGG ACTGG(1/-1)
BstEII 1635 GGTGACC G^GT[ACGT]ACC
BstPI 1635 GGTGACC G^GT[ACGT]ACC
Bsu36I 1415 CCTCAGG CC^T[ACGT]AGG
CfoI 729 GCGC GCG^C
DraI 874 TTTAAA TTT^AAA
DraIII 1663 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1529 CCGTGG C^C[GA][CT]GG
Eam1105I 1875 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1415 CCTCAGG CC^T[ACGT]AGG
EcoNI 669 CCTTAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1635 GGTGACC G^GT[ACGT]ACC
HhaI 729 GCGC GCG^C
HinP1I 729 GCGC G^CGC
HindIII 1558 AAGCTT A^AGCTT
MamI 1140 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 197 GCCGGC GCC^GGC
NgoMIV 197 GCCGGC G^CCGGC
PflFI 805 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1545 ACCGGT A^CCGGT
PmaCI 1666 CACGTG CAC^GTG
PmlI 1666 CACGTG CAC^GTG
PstI 1790 CTGCAG CTGCA^G
RsaI 255 GTAC GT^AC
ScaI 254 AGTACT AGT^ACT
SfcI 1790 CTGCAG C^T[GA][CT]AG
Tsp45I 1636 GTGAC ^GT[GC]AC
Tsp509I 1171 AATT ^AATT
Tth111I 805 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 579 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 90 GGGCCC GGGCC^C
1752 GGGCCC
AvaI 265 CCCGAG C^[CT]CG[GA]G
1748 CTCGGG
BalI 823 TGGCCA TGG^CCA
1259 TGGCCA
BanI 546 GGTGCC G^G[CT][GA]CC
840 GGTGCC
BsgI 1355 GTGCAG GTGCAG(16/14)
1690 GTGCAG
BsiEI 195 CGGCCG CG[GA][CT]^CG
394 CGGCCG
BsmAI 1338 GAGAC GTCTC(1/5)
512 GTCTC
BsoBI 265 CCCGAG C^[CT]CG[GA]G
1748 CTCGGG
BsrBI 1210 CCGCTC CCGCTC(-3/-3)
1507 GAGCGG
BstAPI 141 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1044 GCAGGAGCTGC
EagI 195 CGGCCG C^GGCCG
394 CGGCCG
EarI 69 CTCTTC CTCTTC(1/4)
6 GAAGAG
EclXI 195 CGGCCG C^GGCCG
394 CGGCCG
Eco52I 195 CGGCCG C^GGCCG
394 CGGCCG
Ksp632I 69 CTCTTC CTCTTC(1/4)
6 GAAGAG
MaeII 401 ACGT A^CGT
1667 ACGT
MaeIII 457 GTTAC ^GT[ACGT]AC
1636 GTGAC
MluNI 823 TGGCCA TGG^CCA
1259 TGGCCA
MscI 823 TGGCCA TGG^CCA
1259 TGGCCA
MseI 671 TTAA T^TAA
875 TTAA
SmlI 976 CTCAAG C^T[CT][GA]AG
1579 CTCAAG
StuI 666 AGGCCT AGG^CCT
869 AGGCCT
Tru9I 671 TTAA T^TAA
875 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BspMI BsrFI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII Sau3AI Sau96I ScrFI
SfaNI SphI StyI TaqI TfiI
TseI TspRI TthHB8I Van91I XhoII