(Circular) (Six-base) MAPSORT of: pFN21AE0040.seq Check: 4880 from: 1 to: 6613 pFN21AE0040 6613 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3036 3036 Size: 6613 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4423 278 Size: 53 83 186 936 2887 2468 Fragments arranged by size: 2887 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3956 1937 Size: 2019 4594 Acc65I G'GTAC_C Cuts at: 3934 3934 Size: 6613 AclI AA'CG_TT Cuts at: 1563 4740 5113 1563 Size: 3177 373 3063 Fragments arranged by size: 3177 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2489 3513 4676 6060 6328 1632 Size: 857 1024 1163 1384 268 1917 Fragments arranged by size: 1917 1384 1163 1024 857 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6416 AflIII A'CryG_T Cuts at: 1203 3822 5512 6489 1203 Size: 2619 1690 977 1327 Fragments arranged by size: 2619 1690 1327 977 AgeI A'CCGG_T Cuts at: 3550 5444 3550 Size: 1894 4719 AhdI GACnn_n'nnGTC Cuts at: 3885 5342 3885 Size: 1457 5156 AleI CACnn'nnGTG Cuts at: 1166 2549 3767 1166 Size: 1383 1218 4012 Fragments arranged by size: 4012 1383 1218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2972 1184 Size: 1788 4825 AlwNI CAG_nnn'CTG Cuts at: 2152 2437 3055 3322 4356 5928 2152 Size: 285 618 267 1034 1572 2837 Fragments arranged by size: 2837 1572 1034 618 285 267 ApaI G_GGCC'C Cuts at: 2099 3761 2099 Size: 1662 4951 ApaLI G'TGCA_C Cuts at: 4670 5826 4670 Size: 1156 5457 ApoI r'AATT_y Cuts at: 1493 3922 4148 4300 6409 1493 Size: 2429 226 152 2109 1697 Fragments arranged by size: 2429 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5167 160 Size: 872 4135 1606 Fragments arranged by size: 4135 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6613 AvaI C'yCGr_G Cuts at: 1870 1943 2270 3753 3938 1870 Size: 73 327 1483 185 4545 Fragments arranged by size: 4545 1483 327 185 73 BamHI G'GATC_C Cuts at: 3943 4310 3943 Size: 367 6246 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2551 2845 3934 5389 618 Size: 325 217 627 764 294 1089 1455 1842 Fragments arranged by size: 1842 1455 1089 764 627 325 294 217 BanII G_rGCy'C Cuts at: 1391 2099 3754 3761 3932 1391 Size: 708 1655 7 171 4072 Fragments arranged by size: 4072 1655 708 171 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5642 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 4804 4838 1903 Size: 34 1892 34 941 34 3678 Fragments arranged by size: 3678 1892 941 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3022 4505 5715 3022 Size: 1483 1210 3920 Fragments arranged by size: 3920 1483 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6613 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3617 5224 136 Size: 107 122 71 631 605 1945 1607 1525 Fragments arranged by size: 1945 1607 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6608 6608 Size: 6613 BlpI GC'TnA_GC Cuts at: 4030 4030 Size: 6613 Bme1580I G_kGCm'C Cuts at: 1790 2099 3761 4674 5830 1790 Size: 309 1662 913 1156 2573 Fragments arranged by size: 2573 1662 1156 913 309 BmgBI CAC'GTC Cuts at: 1318 2407 1318 Size: 1089 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4344 5302 442 Size: 416 53 83 399 24 2927 958 1753 Fragments arranged by size: 2927 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6613 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2414 2858 3238 3642 3722 5273 1239 Size: 1175 444 380 404 80 1551 2579 Fragments arranged by size: 2579 1551 1175 444 404 380 80 Bpu10I CC'TnA_GC Cuts at: 3389 3680 3389 Size: 291 6322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2966 3569 4738 5603 5901 2966 Size: 603 1169 865 298 3678 Fragments arranged by size: 3678 1169 865 603 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2522 5276 882 Size: 617 1023 2754 2219 Fragments arranged by size: 2754 2219 1023 617 BsaAI yAC'GTr Cuts at: 493 1204 3673 4404 493 Size: 711 2469 731 2702 Fragments arranged by size: 2702 2469 731 711 BsaBI GATnn'nnATC Cuts at: 3149 6607 3149 Size: 3458 3155 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4420 4802 5437 275 Size: 53 83 186 936 2887 382 635 1451 Fragments arranged by size: 2887 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3550 5046 5444 5718 5865 6276 3550 Size: 1496 398 274 147 411 3887 Fragments arranged by size: 3887 1496 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6388 Fragments arranged by size: 6388 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2333 2867 3294 3872 1186 Size: 1102 45 534 427 578 3927 Fragments arranged by size: 3927 1102 578 534 427 45 BseYI C'CCAG_C Cuts at: 2079 2424 2499 2629 3035 3312 3322 3429 3612 Size: 345 75 130 406 277 10 107 183 Cuts at: 3612 5816 6312 2079 Size: 2204 496 2380 Fragments arranged by size: 2380 2204 496 406 345 277 183 130 107 75 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3381 3716 1163 Size: 573 1645 335 4060 Fragments arranged by size: 4060 1645 573 335 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2203 2402 4063 4824 5852 1540 Size: 275 186 202 199 1661 761 1028 2301 Fragments arranged by size: 2301 1661 1028 761 275 202 199 186 BsiHKAI G_wGCw'C Cuts at: 4674 4759 5830 4674 Size: 85 1071 5457 Fragments arranged by size: 5457 1071 85 BsmI GAATG_Cn' Cuts at: 4124 4217 4124 Size: 93 6520 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 3754 3761 3932 4674 4759 5830 Size: 399 309 1655 7 171 742 85 1071 Cuts at: 5830 1391 Size: 2174 Fragments arranged by size: 2174 1655 1071 742 399 309 171 85 7 BspEI T'CCGG_A Cuts at: 6276 6276 Size: 6613 BspHI T'CATG_A Cuts at: 4502 6232 4502 Size: 1730 4883 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2226 2313 2954 3036 3968 844 Size: 1382 87 641 82 932 3489 Fragments arranged by size: 3489 1382 932 641 87 82 BsrBI CCG'CTC Cuts at: 1394 1823 3217 3514 4500 1394 Size: 429 1394 297 986 3507 Fragments arranged by size: 3507 1394 986 429 297 BsrDI GCAATG_nn' Cuts at: 65 1248 5108 5282 65 Size: 1183 3860 174 1396 Fragments arranged by size: 3860 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2202 3135 3550 5257 5444 1700 Size: 110 392 933 415 1707 187 2869 Fragments arranged by size: 2869 1707 933 415 392 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6613 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6613 BssSI C'ACGA_G Cuts at: 4673 5685 4673 Size: 1012 5601 BstAPI GCAn_nnn'nTGC Cuts at: 794 2152 3055 794 Size: 1358 903 4352 Fragments arranged by size: 4352 1358 903 BstBI TT'CG_AA Cuts at: 5417 5417 Size: 6613 BstEII G'GTnAC_C Cuts at: 1184 3640 1184 Size: 2456 4157 BstXI CCAn_nnnn'nTGG Cuts at: 2629 3262 3402 3620 3769 2629 Size: 633 140 218 149 5473 Fragments arranged by size: 5473 633 218 149 140 BstYI r'GATC_y Cuts at: 1972 2223 2322 2367 3732 3943 4310 4695 4712 Size: 251 99 45 1365 211 367 385 17 Cuts at: 4712 6164 6250 6262 6608 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1365 385 367 346 251 211 99 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 3421 3421 Size: 6613 BtgI C'CryG_G Cuts at: 513 1065 3534 513 Size: 552 2469 3592 Fragments arranged by size: 3592 2469 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5874 Fragments arranged by size: 5874 683 56 BtsI GCAGTG_nn' Cuts at: 2831 3804 4129 4923 4943 2831 Size: 973 325 794 20 4501 Fragments arranged by size: 4501 973 794 325 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6613 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3865 3900 529 Size: 35 3301 35 3242 Fragments arranged by size: 3301 3242 35 35 DraI TTT'AAA Cuts at: 2881 3917 2881 Size: 1036 5577 DraIII CAC_nnn'GTG Cuts at: 3673 3673 Size: 6613 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5620 809 Size: 507 4304 1802 Fragments arranged by size: 4304 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2200 2399 2828 Size: 54 1425 50 275 18 370 199 429 Cuts at: 2828 3264 3801 4060 4949 6578 8 Size: 436 537 259 889 1629 43 Fragments arranged by size: 1629 1425 889 537 436 429 370 275 259 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2200 2399 4060 1537 Size: 275 388 199 1661 4090 Fragments arranged by size: 4090 1661 388 275 199 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 2080 4543 1353 Size: 653 74 2463 3423 Fragments arranged by size: 3423 2463 653 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2155 2167 5200 5574 5720 387 Size: 1064 138 566 12 3033 374 146 1280 Fragments arranged by size: 3033 1280 1064 566 374 146 138 12 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2414 2489 2858 3238 3513 3642 3722 Size: 393 782 75 369 380 275 129 80 Cuts at: 3722 4676 5273 6060 6328 1239 Size: 954 597 787 268 1524 Fragments arranged by size: 1524 954 787 782 597 393 380 369 275 268 129 80 75 EcoNI CCTnn'n_nnAGG Cuts at: 2678 2678 Size: 6613 EcoO109I rG'GnC_Cy Cuts at: 2095 2281 2716 2943 3039 3211 3289 3425 3757 Size: 186 435 227 96 172 78 136 332 Cuts at: 3757 3758 3781 2095 Size: 1 23 4927 Fragments arranged by size: 4927 435 332 227 186 172 136 96 78 23 1 EcoRI G'AATT_C Cuts at: 3922 4300 3922 Size: 378 6235 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2791 2791 Size: 6613 FspI TGC'GCA Cuts at: 5119 5119 Size: 6613 HaeII r_GCGC'y Cuts at: 1678 5760 1678 Size: 4082 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2843 2875 3273 3289 3305 Size: 32 135 32 1271 32 398 16 16 Cuts at: 3305 3321 3362 3394 3871 3903 5254 5286 5328 Size: 16 41 32 477 32 1351 32 42 Cuts at: 5328 5360 1373 Size: 32 2626 Fragments arranged by size: 2626 1351 1271 477 398 135 42 41 32 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 3957 4203 669 Size: 988 281 2019 246 3079 Fragments arranged by size: 3079 2019 988 281 246 HindIII A'AGCT_T Cuts at: 748 3563 748 Size: 2815 3798 HpaI GTT'AAC Cuts at: 4203 4203 Size: 6613 KpnI G_GTAC'C Cuts at: 3938 3938 Size: 6613 MfeI C'AATT_G Cuts at: 4212 4212 Size: 6613 MluI A'CGCG_T Cuts at: 6489 6489 Size: 6613 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1997 3834 5727 5911 1305 Size: 692 1837 1893 184 2007 Fragments arranged by size: 2007 1893 1837 692 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2830 3266 10 Size: 54 1425 343 998 436 3357 Fragments arranged by size: 3357 1425 998 436 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2549 2921 2937 3692 3767 4571 4930 Size: 648 1383 372 16 755 75 804 359 Cuts at: 4930 5089 518 Size: 159 2042 Fragments arranged by size: 2042 1383 804 755 648 372 359 159 75 16 MspA1I CmG'CkG Cuts at: 1606 2287 2434 2969 3319 3616 3652 3700 3892 Size: 681 147 535 350 297 36 48 192 Cuts at: 3892 4029 4706 5854 6099 1606 Size: 137 677 1148 245 2120 Fragments arranged by size: 2120 1148 681 677 535 350 297 245 192 147 137 48 36 NaeI GCC'GGC Cuts at: 2204 2204 Size: 6613 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6061 NdeI CA'TA_TG Cuts at: 387 387 Size: 6613 NgoMIV G'CCGG_C Cuts at: 2202 2202 Size: 6613 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6613 NotI GC'GGCC_GC Cuts at: 4060 4060 Size: 6613 NspI r_CATG'y Cuts at: 1132 1229 2165 2269 3104 3112 3826 3971 5516 Size: 97 936 104 835 8 714 145 1545 Cuts at: 5516 1132 Size: 2229 Fragments arranged by size: 2229 1545 936 835 714 145 104 97 8 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6613 PciI A'CATG_T Cuts at: 3822 5512 3822 Size: 1690 4923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2437 3273 3673 1108 Size: 1329 836 400 4048 Fragments arranged by size: 4048 1329 836 400 PfoI T'CCnGG_A Cuts at: 2691 2691 Size: 6613 PmeI GTTT'AAAC Cuts at: 3917 3917 Size: 6613 PmlI CAC'GTG Cuts at: 3673 3673 Size: 6613 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4682 4714 6195 6227 4682 Size: 32 1481 32 5068 Fragments arranged by size: 5068 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2281 2943 3211 3289 3425 2281 Size: 662 268 78 136 5469 Fragments arranged by size: 5469 662 268 136 78 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6613 PsiI TTA'TAA Cuts at: 4183 4183 Size: 6613 PspOMI G'GGCC_C Cuts at: 2095 3757 2095 Size: 1662 4951 PstI C_TGCA'G Cuts at: 830 3799 3965 830 Size: 2969 166 3478 Fragments arranged by size: 3478 2969 166 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6613 PvuII CAG'CTG Cuts at: 2969 3616 3652 3700 2969 Size: 647 36 48 5882 Fragments arranged by size: 5882 647 48 36 SalI G'TCGA_C Cuts at: 1936 3955 1936 Size: 2019 4594 SanDI GG'GwC_CC Cuts at: 2281 3211 3289 2281 Size: 930 78 5605 Fragments arranged by size: 5605 930 78 SbfI CC_TGCA'GG Cuts at: 3965 3965 Size: 6613 ScaI AGT'ACT Cuts at: 2261 4861 6573 2261 Size: 2600 1712 2301 Fragments arranged by size: 2600 2301 1712 SfcI C'TryA_G Cuts at: 826 1045 3795 3961 5096 5777 5968 826 Size: 219 2750 166 1135 681 191 1471 Fragments arranged by size: 2750 1471 1135 681 219 191 166 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6613 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6613 SmaI CCC'GGG Cuts at: 1872 3940 1872 Size: 2068 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2981 3584 4717 5618 5880 820 Size: 197 926 1038 603 1133 901 262 1553 Fragments arranged by size: 1553 1133 1038 926 901 603 262 197 SnaBI TAC'GTA Cuts at: 493 4404 493 Size: 3911 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6613 SphI G_CATG'C Cuts at: 1132 2165 2269 3104 3971 1132 Size: 1033 104 835 867 3774 Fragments arranged by size: 3774 1033 867 835 104 SspI AAT'ATT Cuts at: 5 52 4537 5 Size: 47 4485 2081 Fragments arranged by size: 4485 2081 47 StuI AGG'CCT Cuts at: 1517 2673 2876 1517 Size: 1156 203 5254 Fragments arranged by size: 5254 1156 203 StyI C'CwwG_G Cuts at: 513 1065 2871 2896 3636 4052 513 Size: 552 1806 25 740 416 3074 Fragments arranged by size: 3074 1806 740 552 416 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4640 4657 4810 4995 5440 325 Size: 1255 3060 17 153 185 445 1498 Fragments arranged by size: 3060 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 4859 6571 Size: 274 80 33 51 1443 282 2600 1712 Cuts at: 6571 96 Size: 138 Fragments arranged by size: 2600 1712 1443 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3338 3370 6573 6605 3338 Size: 32 3203 32 3346 Fragments arranged by size: 3346 3203 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2813 1316 Size: 1497 5116 XbaI T'CTAG_A Cuts at: 3949 3949 Size: 6613 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6613 XmaI C'CCGG_G Cuts at: 1870 3938 1870 Size: 2068 4545 XmnI GAAnn'nnTTC Cuts at: 2588 4742 2588 Size: 2154 4459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4421 276 Size: 53 83 186 936 2887 2468 Fragments arranged by size: 2887 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI