Restriction map for
pFN21AE0040
(Linear) (Six-base) MAPSORT of: KIEE0040.seq Check: 6835 from: 1 to: 1908
KIEE0040.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1031 1908
Size: 1031 877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1508 1908
Size: 484 1024 400
Fragments arranged by size:
1024 484 400
AflIII A'CryG_T
Cuts at: 0 1817 1908
Size: 1817 91
AgeI A'CCGG_T
Cuts at: 0 1545 1908
Size: 1545 363
AhdI GACnn_n'nnGTC
Cuts at: 0 1880 1908
Size: 1880 28
AleI CACnn'nnGTG
Cuts at: 0 544 1762 1908
Size: 544 1218 146
Fragments arranged by size:
1218 544 146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 967 1908
Size: 967 941
AlwNI CAG_nnn'CTG
Cuts at: 0 147 432 1050 1317 1908
Size: 147 285 618 267 591
Fragments arranged by size:
618 591 285 267 147
ApaI G_GGCC'C
Cuts at: 0 94 1756 1908
Size: 94 1662 152
Fragments arranged by size:
1662 152 94
AvaI C'yCGr_G
Cuts at: 0 265 1748 1908
Size: 265 1483 160
Fragments arranged by size:
1483 265 160
BanI G'GyrC_C
Cuts at: 0 546 840 1908
Size: 546 294 1068
Fragments arranged by size:
1068 546 294
BanII G_rGCy'C
Cuts at: 0 94 1749 1756 1908
Size: 94 1655 7 152
Fragments arranged by size:
1655 152 94 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1824 1858 1908
Size: 1824 34 50
Fragments arranged by size:
1824 50 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1017 1908
Size: 1017 891
BglI GCCn_nnn'nGGC
Cuts at: 0 1612 1908
Size: 1612 296
Bme1580I G_kGCm'C
Cuts at: 0 94 1756 1908
Size: 94 1662 152
Fragments arranged by size:
1662 152 94
BmgBI CAC'GTC
Cuts at: 0 402 1908
Size: 402 1506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 853 1233 1637 1717 1908
Size: 409 444 380 404 80 191
Fragments arranged by size:
444 409 404 380 191 80
Bpu10I CC'TnA_GC
Cuts at: 0 1384 1675 1908
Size: 1384 291 233
Fragments arranged by size:
1384 291 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1564 1908
Size: 961 603 344
Fragments arranged by size:
961 603 344
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 1908
Size: 517 1391
BsaAI yAC'GTr
Cuts at: 0 1668 1908
Size: 1668 240
BsaBI GATnn'nnATC
Cuts at: 0 1144 1908
Size: 1144 764
BsaWI w'CCGG_w
Cuts at: 0 1545 1908
Size: 1545 363
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 328 862 1289 1867 1908
Size: 283 45 534 427 578 41
Fragments arranged by size:
578 534 427 283 45 41
BseYI C'CCAG_C
Cuts at: 0 74 419 494 624 1030 1307 1317 1424
Size: 74 345 75 130 406 277 10 107
Cuts at: 1424 1607 1908
Size: 183 301
Fragments arranged by size:
406 345 301 277 183 130 107 75
74 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1376 1711 1908
Size: 1376 335 197
Fragments arranged by size:
1376 335 197
BsiEI CG_ry'CG
Cuts at: 0 198 397 1908
Size: 198 199 1511
Fragments arranged by size:
1511 199 198
Bsp1286I G_dGCh'C
Cuts at: 0 94 1749 1756 1908
Size: 94 1655 7 152
Fragments arranged by size:
1655 152 94 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 221 308 949 1031 1908
Size: 221 87 641 82 877
Fragments arranged by size:
877 641 221 87 82
BsrBI CCG'CTC
Cuts at: 0 1212 1509 1908
Size: 1212 297 399
Fragments arranged by size:
1212 399 297
BsrFI r'CCGG_y
Cuts at: 0 197 1130 1545 1908
Size: 197 933 415 363
Fragments arranged by size:
933 415 363 197
BstAPI GCAn_nnn'nTGC
Cuts at: 0 147 1050 1908
Size: 147 903 858
Fragments arranged by size:
903 858 147
BstEII G'GTnAC_C
Cuts at: 0 1635 1908
Size: 1635 273
BstXI CCAn_nnnn'nTGG
Cuts at: 0 624 1257 1397 1615 1764 1908
Size: 624 633 140 218 149 144
Fragments arranged by size:
633 624 218 149 144 140
BstYI r'GATC_y
Cuts at: 0 218 317 362 1727 1908
Size: 218 99 45 1365 181
Fragments arranged by size:
1365 218 181 99 45
Bsu36I CC'TnA_GG
Cuts at: 0 1416 1908
Size: 1416 492
BtgI C'CryG_G
Cuts at: 0 1529 1908
Size: 1529 379
BtsI GCAGTG_nn'
Cuts at: 0 826 1799 1908
Size: 826 973 109
Fragments arranged by size:
973 826 109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1860 1895 1908
Size: 1860 35 13
Fragments arranged by size:
1860 35 13
DraI TTT'AAA
Cuts at: 0 876 1908
Size: 876 1032
DraIII CAC_nnn'GTG
Cuts at: 0 1668 1908
Size: 1668 240
EaeI y'GGCC_r
Cuts at: 0 195 394 823 1259 1796 1908
Size: 195 199 429 436 537 112
Fragments arranged by size:
537 436 429 199 195 112
EagI C'GGCC_G
Cuts at: 0 195 394 1908
Size: 195 199 1514
Fragments arranged by size:
1514 199 195
EarI CTCTTCn'nnn_
Cuts at: 0 1 75 1908
Size: 1 74 1833
Fragments arranged by size:
1833 74 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 150 162 1908
Size: 150 12 1746
Fragments arranged by size:
1746 150 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 484 853 1233 1508 1637 1717 1908
Size: 409 75 369 380 275 129 80 191
Fragments arranged by size:
409 380 369 275 191 129 80 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 673 1908
Size: 673 1235
EcoO109I rG'GnC_Cy
Cuts at: 0 90 276 711 938 1034 1206 1284 1420
Size: 90 186 435 227 96 172 78 136
Cuts at: 1420 1752 1753 1776 1908
Size: 332 1 23 132
Fragments arranged by size:
435 332 227 186 172 136 132 96
90 78 23 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 786 1908
Size: 786 1122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 838 870 1268 1284 1300 1316 1357 1389
Size: 838 32 398 16 16 16 41 32
Cuts at: 1389 1866 1898 1908
Size: 477 32 10
Fragments arranged by size:
838 477 398 41 32 32 32 16
16 16 10
HindIII A'AGCT_T
Cuts at: 0 1558 1908
Size: 1558 350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1829 1908
Size: 1829 79
MscI TGG'CCA
Cuts at: 0 825 1261 1908
Size: 825 436 647
Fragments arranged by size:
825 647 436
MslI CAynn'nnrTG
Cuts at: 0 544 916 932 1687 1762 1908
Size: 544 372 16 755 75 146
Fragments arranged by size:
755 544 372 146 75 16
MspA1I CmG'CkG
Cuts at: 0 282 429 964 1314 1611 1647 1695 1887
Size: 282 147 535 350 297 36 48 192
Cuts at: 1887 1908
Size: 21
Fragments arranged by size:
535 350 297 282 192 147 48 36
21
NaeI GCC'GGC
Cuts at: 0 199 1908
Size: 199 1709
NgoMIV G'CCGG_C
Cuts at: 0 197 1908
Size: 197 1711
NspI r_CATG'y
Cuts at: 0 160 264 1099 1107 1821 1908
Size: 160 104 835 8 714 87
Fragments arranged by size:
835 714 160 104 87 8
PciI A'CATG_T
Cuts at: 0 1817 1908
Size: 1817 91
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 1268 1668 1908
Size: 432 836 400 240
Fragments arranged by size:
836 432 400 240
PfoI T'CCnGG_A
Cuts at: 0 686 1908
Size: 686 1222
PmlI CAC'GTG
Cuts at: 0 1668 1908
Size: 1668 240
PpuMI rG'GwC_Cy
Cuts at: 0 276 938 1206 1284 1420 1908
Size: 276 662 268 78 136 488
Fragments arranged by size:
662 488 276 268 136 78
PspOMI G'GGCC_C
Cuts at: 0 90 1752 1908
Size: 90 1662 156
Fragments arranged by size:
1662 156 90
PstI C_TGCA'G
Cuts at: 0 1794 1908
Size: 1794 114
PvuII CAG'CTG
Cuts at: 0 964 1611 1647 1695 1908
Size: 964 647 36 48 213
Fragments arranged by size:
964 647 213 48 36
SanDI GG'GwC_CC
Cuts at: 0 276 1206 1284 1908
Size: 276 930 78 624
Fragments arranged by size:
930 624 276 78
ScaI AGT'ACT
Cuts at: 0 256 1908
Size: 256 1652
SfcI C'TryA_G
Cuts at: 0 1790 1908
Size: 1790 118
SmlI C'TyrA_G
Cuts at: 0 976 1579 1908
Size: 976 603 329
Fragments arranged by size:
976 603 329
SphI G_CATG'C
Cuts at: 0 160 264 1099 1908
Size: 160 104 835 809
Fragments arranged by size:
835 809 160 104
StuI AGG'CCT
Cuts at: 0 668 871 1908
Size: 668 203 1037
Fragments arranged by size:
1037 668 203
StyI C'CwwG_G
Cuts at: 0 866 891 1631 1908
Size: 866 25 740 277
Fragments arranged by size:
866 740 277 25
TatI w'GTAC_w
Cuts at: 0 254 1908
Size: 254 1654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1333 1365 1908
Size: 1333 32 543
Fragments arranged by size:
1333 543 32
Tth111I GACn'n_nGTC
Cuts at: 0 808 1908
Size: 808 1100
XmnI GAAnn'nnTTC
Cuts at: 0 583 1908
Size: 583 1325
Enzymes that do cut:
AarI AcuI AflIII AgeI AhdI AleI AlfI AlwNI
ApaI AvaI BanI BanII BcgI BciVI BglI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
DraI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI Hin4I HindIII MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PciI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SanDI ScaI SfcI SmlI SphI
StuI StyI TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AloI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BclI BfrBI BglII BlpI BmrI BmtI
BplI BsaHI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstZ17I BtgZI
ClaI DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TaqII XbaI XcmI XhoI XmaI ZraI