Restriction map for
pFN21AB7917
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BalI BamHI BbrPI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT14I
EcoT22I FseI FspI HincII HindII
HindIII HinfI HpaI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI TfiI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 1005 GAATTC [GA]^AATT[CT]
AflIII 569 ACACGT A^C[GA][CT]GT
AlwNI 28 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 89 GGGCCC GGGCC^C
ApoI 1005 GAATTC [GA]^AATT[CT]
AseI 931 ATTAAT AT^TAAT
AsnI 931 ATTAAT AT^TAAT
Asp700I 330 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1078 CCTCAGC CCTCAGC(-5/-2)
BclI 524 TGATCA T^GATCA
BglI 65 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 654 CGTCTC CGTCTC(1/5)
BsrDI 255 CATTGC GCAATG(2/0)
BssHII 74 GCGCGC G^CGCGC
BssSI 664 CACGAG CACGAG(-5/-1)
BstEII 594 GGTCACC G^GT[ACGT]ACC
BstPI 594 GGTCACC G^GT[ACGT]ACC
BstYI 551 AGATCC [GA]^GATC[CT]
DdeI 1079 CTCAG C^T[ACGT]AG
DraII 88 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 610 GACTGGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 19 TGGCCG [CT]^GGCC[GA]
EcoNI 600 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 88 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 594 GGTCACC G^GT[ACGT]ACC
EcoRI 1005 GAATTC G^AATTC
FbaI 524 TGATCA T^GATCA
HgaI 55 GCGTC GACGC(5/10)
MflI 551 AGATCC [GA]^GATC[CT]
NheI 990 GCTAGC G^CTAGC
NspI 615 GCATGT [GA]CATG^[CT]
PshBI 931 ATTAAT AT^TAAT
PstI 604 CTGCAG CTGCA^G
PvuII 31 CAGCTG CAG^CTG
SbfI 603 CCTGCAGG CCTGCA^GG
SexAI 179 ACCTGGT A^CC[AT]GGT
SfcI 604 CTGCAG C^T[GA][CT]AG
Sse8387I 603 CCTGCAGG CCTGCA^GG
XbaI 777 TCTAGA T^CTAGA
XhoII 551 AGATCC [GA]^GATC[CT]
XmnI 330 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 75 CGCG CG^CG
94 CGCG
AfaI 280 GTAC GT^AC
573 GTAC
AhdI 49 GACGAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
262 GACTTCTTGTC
AlwI 150 GATCC GGATC(4/5)
552 GATCC
AspEI 49 GACGAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
262 GACTTCTTGTC
AspHI 141 GTGCTC G[AT]GC[AT]^C
355 GAGCAC
BbeI 7 GGCGCC GGCGC^C
1043 GGCGCC
BbsI 790 GAAGAC GAAGAC(2/6)
514 GTCTTC
BcgI 96 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1032 GCAAGAGCTTCG
BfaI 778 CTAG C^TAG
991 CTAG
BpuAI 790 GAAGAC GAAGAC(2/6)
514 GTCTTC
BsaAI 570 CACGTA [CT]AC^GT[GA]
629 CACGTA
BsaI 400 GAGACC GGTCTC(1/5)
667 GAGACC
BsiHKAI 141 GTGCTC G[AT]GC[AT]^C
355 GAGCAC
BsmFI 410 GGGAC GGGAC(10/14)
700 GTCCC
BstUI 75 CGCG CG^CG
94 CGCG
Eam1105I 49 GACGAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
262 GACTTCTTGTC
KasI 7 GGCGCC G^GCGCC
1043 GGCGCC
MaeI 778 CTAG C^TAG
991 CTAG
MseI 869 TTAA T^TAA
932 TTAA
MslI 283 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
981 CATATCAGTG
MvnI 75 CGCG CG^CG
94 CGCG
NarI 7 GGCGCC GG^CGCC
1043 GGCGCC
PflMI 27 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
174 CCACAACCTGG
RsaI 280 GTAC GT^AC
573 GTAC
SfoI 7 GGCGCC GGC^GCC
1043 GGCGCC
TaqI 398 TCGA T^CGA
731 TCGA
Tru9I 869 TTAA T^TAA
932 TTAA
Tsp45I 424 GTGAC ^GT[GC]AC
595 GTCAC
TthHB8I 398 TCGA T^CGA
731 TCGA
Van91I 27 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
174 CCACAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcyI AluI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp509I TspRI