(Circular) (Six-base) MAPSORT of: pFN21AB7917.seq Check: 1171 from: 1 to: 5815 pFN21AB7917 5815 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3625 278 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3158 1937 Size: 1221 4594 Acc65I G'GTAC_C Cuts at: 3136 3136 Size: 5815 AclI AA'CG_TT Cuts at: 1563 3942 4315 1563 Size: 2379 373 3063 Fragments arranged by size: 3063 2379 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2149 2502 3878 5262 5530 1632 Size: 517 353 1376 1384 268 1917 Fragments arranged by size: 1917 1384 1376 517 353 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5618 AflIII A'CryG_T Cuts at: 1203 2574 4714 5691 1203 Size: 1371 2140 977 1327 Fragments arranged by size: 2140 1371 1327 977 AgeI A'CCGG_T Cuts at: 4646 4646 Size: 5815 AhdI GACnn_n'nnGTC Cuts at: 2059 2272 4544 2059 Size: 213 2272 3330 Fragments arranged by size: 3330 2272 213 AleI CACnn'nnGTG Cuts at: 1166 2292 1166 Size: 1126 4689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5815 AlwNI CAG_nnn'CTG Cuts at: 2038 3558 5130 2038 Size: 1520 1572 2723 Fragments arranged by size: 2723 1572 1520 ApaI G_GGCC'C Cuts at: 2098 2098 Size: 5815 ApaLI G'TGCA_C Cuts at: 3872 5028 3872 Size: 1156 4659 ApoI r'AATT_y Cuts at: 1493 3010 3124 3350 3502 5611 1493 Size: 1517 114 226 152 2109 1697 Fragments arranged by size: 2109 1697 1517 226 152 114 AseI AT'TA_AT Cuts at: 160 1032 2937 4369 160 Size: 872 1905 1432 1606 Fragments arranged by size: 1905 1606 1432 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 AvaI C'yCGr_G Cuts at: 1870 1943 3140 1870 Size: 73 1197 4545 Fragments arranged by size: 4545 1197 73 BamHI G'GATC_C Cuts at: 3145 3512 3145 Size: 367 5448 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2540 3048 3136 4591 Size: 325 217 627 225 528 508 88 1455 Cuts at: 4591 618 Size: 1842 Fragments arranged by size: 1842 1455 627 528 508 325 225 217 88 BanII G_rGCy'C Cuts at: 1391 2098 2257 2312 3134 1391 Size: 707 159 55 822 4072 Fragments arranged by size: 4072 822 707 159 55 BbeI G_GCGC'C Cuts at: 2016 3052 2016 Size: 1036 4779 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2512 2802 928 Size: 971 613 290 3941 Fragments arranged by size: 3941 971 613 290 BbvCI CC'TCA_GC Cuts at: 3084 3084 Size: 5815 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2090 2124 3026 3060 4006 4040 1903 Size: 34 153 34 902 34 946 34 3678 Fragments arranged by size: 3678 946 902 153 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3707 4917 3707 Size: 1210 4605 BclI T'GATC_A Cuts at: 1548 2529 1548 Size: 981 4834 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2076 4426 136 Size: 107 122 71 631 605 404 2350 1525 Fragments arranged by size: 2350 1525 631 605 404 122 107 71 BglII A'GATC_T Cuts at: 5810 5810 Size: 5815 BlpI GC'TnA_GC Cuts at: 3232 3232 Size: 5815 Bme1580I G_kGCm'C Cuts at: 1790 2098 3876 5032 1790 Size: 308 1778 1156 2573 Fragments arranged by size: 2573 1778 1156 308 BmgBI CAC'GTC Cuts at: 1318 2659 1318 Size: 1341 4474 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 3546 4504 Size: 416 53 83 399 24 882 1247 958 Cuts at: 4504 442 Size: 1753 Fragments arranged by size: 1753 1247 958 882 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2999 1056 Size: 1943 3872 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2164 2164 Size: 5815 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2120 2510 4475 1239 Size: 821 60 390 1965 2579 Fragments arranged by size: 2579 1965 821 390 60 Bpu10I CC'TnA_GC Cuts at: 3084 3084 Size: 5815 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3940 4805 5103 3940 Size: 865 298 4652 Fragments arranged by size: 4652 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2399 2666 4478 882 Size: 617 900 267 1812 2219 Fragments arranged by size: 2219 1812 900 617 267 BsaAI yAC'GTr Cuts at: 493 1204 2577 2636 3606 493 Size: 711 1373 59 970 2702 Fragments arranged by size: 2702 1373 970 711 59 BsaBI GATnn'nnATC Cuts at: 5809 5809 Size: 5815 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 2060 3049 3622 Size: 53 83 186 936 480 47 989 573 Cuts at: 3622 4004 4639 275 Size: 382 635 1451 Fragments arranged by size: 1451 989 936 635 573 480 382 186 83 53 47 BsaWI w'CCGG_w Cuts at: 4248 4646 4920 5067 5478 4248 Size: 398 274 147 411 4585 Fragments arranged by size: 4585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2688 2718 1178 Size: 30 165 30 1285 30 4275 Fragments arranged by size: 4275 1285 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5815 BseYI C'CCAG_C Cuts at: 2073 2211 2595 2775 5018 5514 2073 Size: 138 384 180 2243 496 2374 Fragments arranged by size: 2374 2243 496 384 180 138 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5242 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3265 4026 5054 1540 Size: 275 186 1264 761 1028 2301 Fragments arranged by size: 2301 1264 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2150 2364 3876 3961 5032 2150 Size: 214 1512 85 1071 2933 Fragments arranged by size: 2933 1512 1071 214 85 BsmI GAATG_Cn' Cuts at: 3326 3419 3326 Size: 93 5722 BsmBI CGTCTCn'nnnn_ Cuts at: 2665 2665 Size: 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2150 2257 2312 2364 3134 3876 Size: 399 308 52 107 55 52 770 742 Cuts at: 3876 3961 5032 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 770 742 399 308 107 85 55 52 52 BspEI T'CCGG_A Cuts at: 5478 5478 Size: 5815 BspHI T'CATG_A Cuts at: 3704 5434 3704 Size: 1730 4085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3170 844 Size: 2326 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3702 1394 Size: 429 1879 3507 Fragments arranged by size: 3507 1879 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2259 3118 4310 4484 65 Size: 1183 1011 859 1192 174 1396 Fragments arranged by size: 1396 1192 1183 1011 859 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4459 4646 1700 Size: 110 2649 187 2869 Fragments arranged by size: 2869 2649 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5815 BssHII G'CGCG_C Cuts at: 1925 2079 1925 Size: 154 5661 BssSI C'ACGA_G Cuts at: 2669 3875 4887 2669 Size: 1206 1012 3597 Fragments arranged by size: 3597 1206 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5815 BstBI TT'CG_AA Cuts at: 4619 4619 Size: 5815 BstEII G'GTnAC_C Cuts at: 1184 2599 1184 Size: 1415 4400 BstYI r'GATC_y Cuts at: 1972 2556 3145 3512 3897 3914 5366 5452 5464 Size: 584 589 367 385 17 1452 86 12 Cuts at: 5464 5810 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 589 584 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4324 Fragments arranged by size: 4324 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2158 2630 487 Size: 683 56 932 472 3672 Fragments arranged by size: 3672 932 683 472 56 BtsI GCAGTG_nn' Cuts at: 2432 3331 4125 4145 2432 Size: 899 794 20 4102 Fragments arranged by size: 4102 899 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5815 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5780 DraI TTT'AAA Cuts at: 3119 3119 Size: 5815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2621 4822 809 Size: 507 1305 2201 1802 Fragments arranged by size: 2201 1802 1305 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2024 3262 4151 Size: 54 1425 50 275 18 194 1238 889 Cuts at: 4151 5780 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1238 889 275 194 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3262 1537 Size: 275 1450 4090 Fragments arranged by size: 4090 1450 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3745 1353 Size: 2392 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4402 4776 4922 387 Size: 1064 138 2813 374 146 1280 Fragments arranged by size: 2813 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2120 2149 2502 2510 3878 4475 Size: 393 428 60 29 353 8 1368 597 Cuts at: 4475 5262 5530 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1368 787 597 428 393 353 268 60 29 8 EcoNI CCTnn'n_nnAGG Cuts at: 2609 2609 Size: 5815 EcoO109I rG'GnC_Cy Cuts at: 2094 2094 Size: 5815 EcoRI G'AATT_C Cuts at: 3010 3124 3502 3010 Size: 114 378 5323 Fragments arranged by size: 5323 378 114 FspI TGC'GCA Cuts at: 4321 4321 Size: 5815 HaeII r_GCGC'y Cuts at: 1678 2016 2134 3052 4962 1678 Size: 338 118 918 1910 2531 Fragments arranged by size: 2531 1910 918 338 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 2258 2290 2688 Size: 32 135 32 473 32 181 32 398 Cuts at: 2688 2720 4456 4488 4530 4562 1373 Size: 32 1736 32 42 32 2626 Fragments arranged by size: 2626 1736 473 398 181 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3159 3405 669 Size: 988 281 1221 246 3079 Fragments arranged by size: 3079 1221 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5815 HpaI GTT'AAC Cuts at: 3405 3405 Size: 5815 KasI G'GCGC_C Cuts at: 2012 3048 2012 Size: 1036 4779 KpnI G_GTAC'C Cuts at: 3140 3140 Size: 5815 MfeI C'AATT_G Cuts at: 3414 3414 Size: 5815 MluI A'CGCG_T Cuts at: 5691 5691 Size: 5815 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2371 2706 2743 4929 5113 1305 Size: 1066 335 37 2186 184 2007 Fragments arranged by size: 2186 2007 1066 335 184 37 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3993 Fragments arranged by size: 3993 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2292 2990 3773 4132 4291 518 Size: 648 1126 698 783 359 159 2042 Fragments arranged by size: 2042 1126 783 698 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2073 2211 2443 3231 3908 5056 5301 Size: 432 35 138 232 788 677 1148 245 Cuts at: 5301 1606 Size: 2120 Fragments arranged by size: 2120 1148 788 677 432 245 232 138 35 NarI GG'CG_CC Cuts at: 2013 3049 2013 Size: 1036 4779 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4324 Fragments arranged by size: 4324 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5815 NheI G'CTAG_C Cuts at: 1052 2995 1052 Size: 1943 3872 NotI GC'GGCC_GC Cuts at: 3262 3262 Size: 5815 NspI r_CATG'y Cuts at: 1132 1229 2624 3173 4718 1132 Size: 97 1395 549 1545 2229 Fragments arranged by size: 2229 1545 1395 549 97 PasI CC'CwG_GG Cuts at: 1348 2505 1348 Size: 1157 4658 PciI A'CATG_T Cuts at: 4714 4714 Size: 5815 PflMI CCAn_nnn'nTGG Cuts at: 1108 2038 2185 1108 Size: 930 147 4738 Fragments arranged by size: 4738 930 147 PfoI T'CCnGG_A Cuts at: 2136 2355 2136 Size: 219 5596 PmeI GTTT'AAAC Cuts at: 3119 3119 Size: 5815 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3884 3916 5397 5429 3884 Size: 32 1481 32 4270 Fragments arranged by size: 4270 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5815 PsiI TTA'TAA Cuts at: 3385 3385 Size: 5815 PspOMI G'GGCC_C Cuts at: 2094 2094 Size: 5815 PstI C_TGCA'G Cuts at: 830 2613 3167 830 Size: 1783 554 3478 Fragments arranged by size: 3478 1783 554 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5815 PvuII CAG'CTG Cuts at: 2038 2038 Size: 5815 SalI G'TCGA_C Cuts at: 1936 3157 1936 Size: 1221 4594 SbfI CC_TGCA'GG Cuts at: 2613 3167 2613 Size: 554 5261 ScaI AGT'ACT Cuts at: 4063 5775 4063 Size: 1712 4103 SexAI A'CCwGG_T Cuts at: 2184 2184 Size: 5815 SfcI C'TryA_G Cuts at: 826 1045 2609 3163 4298 4979 5170 826 Size: 219 1564 554 1135 681 191 1471 Fragments arranged by size: 1564 1471 1135 681 554 219 191 SfoI GGC'GCC Cuts at: 2014 3050 2014 Size: 1036 4779 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5815 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5815 SmaI CCC'GGG Cuts at: 1872 3142 1872 Size: 1270 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3919 4820 5082 820 Size: 197 926 1976 901 262 1553 Fragments arranged by size: 1976 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3606 493 Size: 3113 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5815 SphI G_CATG'C Cuts at: 1132 3173 1132 Size: 2041 3774 SspI AAT'ATT Cuts at: 5 52 3739 5 Size: 47 3687 2081 Fragments arranged by size: 3687 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5815 StyI C'CwwG_G Cuts at: 513 1065 2004 3254 513 Size: 552 939 1250 3074 Fragments arranged by size: 3074 1250 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2486 3842 3859 4012 4197 4642 325 Size: 1255 906 1356 17 153 185 445 1498 Fragments arranged by size: 1498 1356 1255 906 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4061 5773 96 Size: 274 80 33 51 1443 2084 1712 138 Fragments arranged by size: 2084 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5775 5807 5775 Size: 32 5783 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5815 XbaI T'CTAG_A Cuts at: 2782 3151 2782 Size: 369 5446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5815 XmaI C'CCGG_G Cuts at: 1870 3140 1870 Size: 1270 4545 XmnI GAAnn'nnTTC Cuts at: 2339 3944 2339 Size: 1605 4210 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3623 276 Size: 53 83 186 936 2089 2468 Fragments arranged by size: 2468 2089 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI