Restriction map for
pFN21AB7917
(Linear) (Six-base) MAPSORT of: KIBB7917.seq Check: 2560 from: 1 to: 1110
KIBB7917.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 497 1110
Size: 144 353 613
Fragments arranged by size:
613 353 144
AflIII A'CryG_T
Cuts at: 0 569 1110
Size: 569 541
AhdI GACnn_n'nnGTC
Cuts at: 0 54 267 1110
Size: 54 213 843
Fragments arranged by size:
843 213 54
AleI CACnn'nnGTG
Cuts at: 0 287 1110
Size: 287 823
AlwNI CAG_nnn'CTG
Cuts at: 0 33 1110
Size: 33 1077
ApaI G_GGCC'C
Cuts at: 0 93 1110
Size: 93 1017
ApoI r'AATT_y
Cuts at: 0 1005 1110
Size: 1005 105
AseI AT'TA_AT
Cuts at: 0 932 1110
Size: 932 178
BanI G'GyrC_C
Cuts at: 0 7 535 1043 1110
Size: 7 528 508 67
Fragments arranged by size:
528 508 67 7
BanII G_rGCy'C
Cuts at: 0 93 252 307 1110
Size: 93 159 55 803
Fragments arranged by size:
803 159 93 55
BbeI G_GCGC'C
Cuts at: 0 11 1047 1110
Size: 11 1036 63
Fragments arranged by size:
1036 63 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 507 797 1110
Size: 507 290 313
Fragments arranged by size:
507 313 290
BbvCI CC'TCA_GC
Cuts at: 0 1079 1110
Size: 1079 31
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 85 119 1021 1055 1110
Size: 85 34 902 34 55
Fragments arranged by size:
902 85 55 34 34
BclI T'GATC_A
Cuts at: 0 524 1110
Size: 524 586
BglI GCCn_nnn'nGGC
Cuts at: 0 71 1110
Size: 71 1039
Bme1580I G_kGCm'C
Cuts at: 0 93 1110
Size: 93 1017
BmgBI CAC'GTC
Cuts at: 0 654 1110
Size: 654 456
BmrI ACTGGGnnnn_n'
Cuts at: 0 294 1110
Size: 294 816
BmtI G_CTAG'C
Cuts at: 0 994 1110
Size: 994 116
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 159 1110
Size: 159 951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 115 505 1110
Size: 55 60 390 605
Fragments arranged by size:
605 390 60 55
Bpu10I CC'TnA_GC
Cuts at: 0 1079 1110
Size: 1079 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 394 661 1110
Size: 394 267 449
Fragments arranged by size:
449 394 267
BsaAI yAC'GTr
Cuts at: 0 572 631 1110
Size: 572 59 479
Fragments arranged by size:
572 479 59
BsaHI Gr'CG_yC
Cuts at: 0 8 55 1044 1110
Size: 8 47 989 66
Fragments arranged by size:
989 66 47 8
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 683 713 1110
Size: 683 30 397
Fragments arranged by size:
683 397 30
BseYI C'CCAG_C
Cuts at: 0 68 206 590 770 1110
Size: 68 138 384 180 340
Fragments arranged by size:
384 340 180 138 68
BsiHKAI G_wGCw'C
Cuts at: 0 145 359 1110
Size: 145 214 751
Fragments arranged by size:
751 214 145
BsmBI CGTCTCn'nnnn_
Cuts at: 0 660 1110
Size: 660 450
Bsp1286I G_dGCh'C
Cuts at: 0 93 145 252 307 359 1110
Size: 93 52 107 55 52 751
Fragments arranged by size:
751 107 93 55 52 52
BsrDI GCAATG_nn'
Cuts at: 0 254 1110
Size: 254 856
BssHII G'CGCG_C
Cuts at: 0 74 1110
Size: 74 1036
BssSI C'ACGA_G
Cuts at: 0 664 1110
Size: 664 446
BstEII G'GTnAC_C
Cuts at: 0 594 1110
Size: 594 516
BstYI r'GATC_y
Cuts at: 0 551 1110
Size: 551 559
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 153 625 1110
Size: 153 472 485
Fragments arranged by size:
485 472 153
BtsI GCAGTG_nn'
Cuts at: 0 427 1110
Size: 427 683
DrdI GACnn_nn'nnGTC
Cuts at: 0 616 1110
Size: 616 494
EaeI y'GGCC_r
Cuts at: 0 19 1110
Size: 19 1091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 115 144 497 505 1110
Size: 55 60 29 353 8 605
Fragments arranged by size:
605 353 60 55 29 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 604 1110
Size: 604 506
EcoO109I rG'GnC_Cy
Cuts at: 0 89 1110
Size: 89 1021
EcoRI G'AATT_C
Cuts at: 0 1005 1110
Size: 1005 105
HaeII r_GCGC'y
Cuts at: 0 11 129 1047 1110
Size: 11 118 918 63
Fragments arranged by size:
918 118 63 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 253 285 683 715 1110
Size: 40 32 181 32 398 32 395
Fragments arranged by size:
398 395 181 40 32 32 32
KasI G'GCGC_C
Cuts at: 0 7 1043 1110
Size: 7 1036 67
Fragments arranged by size:
1036 67 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 701 738 1110
Size: 366 335 37 372
Fragments arranged by size:
372 366 335 37
MslI CAynn'nnrTG
Cuts at: 0 287 985 1110
Size: 287 698 125
Fragments arranged by size:
698 287 125
MspA1I CmG'CkG
Cuts at: 0 33 68 206 438 1110
Size: 33 35 138 232 672
Fragments arranged by size:
672 232 138 35 33
NarI GG'CG_CC
Cuts at: 0 8 1044 1110
Size: 8 1036 66
Fragments arranged by size:
1036 66 8
NheI G'CTAG_C
Cuts at: 0 990 1110
Size: 990 120
NspI r_CATG'y
Cuts at: 0 619 1110
Size: 619 491
PasI CC'CwG_GG
Cuts at: 0 500 1110
Size: 500 610
PflMI CCAn_nnn'nTGG
Cuts at: 0 33 180 1110
Size: 33 147 930
Fragments arranged by size:
930 147 33
PfoI T'CCnGG_A
Cuts at: 0 131 350 1110
Size: 131 219 760
Fragments arranged by size:
760 219 131
PspOMI G'GGCC_C
Cuts at: 0 89 1110
Size: 89 1021
PstI C_TGCA'G
Cuts at: 0 608 1110
Size: 608 502
PvuII CAG'CTG
Cuts at: 0 33 1110
Size: 33 1077
SbfI CC_TGCA'GG
Cuts at: 0 608 1110
Size: 608 502
SexAI A'CCwGG_T
Cuts at: 0 179 1110
Size: 179 931
SfcI C'TryA_G
Cuts at: 0 604 1110
Size: 604 506
SfoI GGC'GCC
Cuts at: 0 9 1045 1110
Size: 9 1036 65
Fragments arranged by size:
1036 65 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 481 1110
Size: 481 629
XbaI T'CTAG_A
Cuts at: 0 777 1110
Size: 777 333
XmnI GAAnn'nnTTC
Cuts at: 0 334 1110
Size: 334 776
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApaI ApoI AseI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaXI BseYI BsiHKAI BsmBI Bsp1286I BsrDI
BssHII BssSI BstEII BstYI BtgZI BtsI DrdI EaeI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MmeI
MslI MspA1I NarI NheI NspI PasI PflMI PfoI
PspOMI PstI PvuII SbfI SexAI SfcI SfoI TaqII
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BciVI BfrBI BglII BlpI BpuEI BsaBI BsaWI
BseRI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII EagI EarI EciI
EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI TstI Tth111I XcmI XhoI
XmaI ZraI