Restriction map for
pFN21AE0512
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BanI BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseRI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
TfiI Tsp509I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 34 GTCTAC GT^[AC][GT]AC
AccII 685 CGCG CG^CG
AocI 631 CCTGAGG CC^T[ACGT]AGG
ApaI 15 GGGCCC GGGCC^C
AspHI 523 GAGCTC G[AT]GC[AT]^C
AvaI 526 CTCGGG C^[CT]CG[GA]G
BamHI 504 GGATCC G^GATCC
BbsI 118 GTCTTC GAAGAC(2/6)
BcgI 518 GCACAGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 499 CTCCAG CTGGAG(16/14)
BpuAI 118 GTCTTC GAAGAC(2/6)
BsiHKAI 523 GAGCTC G[AT]GC[AT]^C
BsmFI 101 GTCCC GGGAC(10/14)
BsoBI 526 CTCGGG C^[CT]CG[GA]G
BspLU11I 590 ACATGT A^CATGT
BstUI 685 CGCG CG^CG
BstYI 504 GGATCC [GA]^GATC[CT]
Bsu36I 631 CCTGAGG CC^T[ACGT]AGG
DraIII 765 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 631 CCTGAGG CC^T[ACGT]AGG
EcoNI 372 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 738 GCGGG CCCGC(4/6)
MaeIII 7 GTGAC ^GT[ACGT]AC
MboII 119 TCTTC GAAGA(8/7)
MflI 504 GGATCC [GA]^GATC[CT]
MseI 611 TTAA T^TAA
MvnI 685 CGCG CG^CG
PflMI 150 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 697 CAGCTG CAG^CTG
SacI 523 GAGCTC GAGCT^C
ScaI 830 AGTACT AGT^ACT
SexAI 596 ACCAGGT A^CC[AT]GGT
SphI 177 GCATGC GCATG^C
StuI 452 AGGCCT AGG^CCT
Tru9I 611 TTAA T^TAA
Tsp45I 7 GTGAC ^GT[GC]AC
Van91I 150 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 331 CCAGACAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 504 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 83 TCCGGA T^CCGGA
317 TCCGGA
AflIII 92 ACACGT A^C[GA][CT]GT
590 ACATGT
AlwNI 694 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
712 CAGGACCTG
BalI 62 TGGCCA TGG^CCA
719 TGGCCA
BcnI 264 CCCGG CC^[GC]GG
774 CCCGG
BseAI 83 TCCGGA T^CCGGA
317 TCCGGA
BsgI 364 CTGCAC GTGCAG(16/14)
702 GTGCAG
BspEI 83 TCCGGA T^CCGGA
317 TCCGGA
BspMI 233 ACCTGC ACCTGC(4/8)
779 ACCTGC
DdeI 632 CTGAG C^T[ACGT]AG
847 CTGAG
DpnII 505 GATC ^GATC
648 GATC
DsaI 227 CCGTGG C^C[GA][CT]GG
458 CCGTGG
EaeI 62 TGGCCA [CT]^GGCC[GA]
719 TGGCCA
HinfI 256 GAGTC G^A[ACGT]TC
472 GACTC
MboI 505 GATC ^GATC
648 GATC
MluNI 62 TGGCCA TGG^CCA
719 TGGCCA
MroI 83 TCCGGA T^CCGGA
317 TCCGGA
MscI 62 TGGCCA TGG^CCA
719 TGGCCA
MspA1I 305 CAGCGG C[AC]G^C[GT]G
697 CAGCTG
NciI 264 CCCGG CC^[GC]GG
774 CCCGG
NdeII 505 GATC ^GATC
648 GATC
NspI 177 GCATGC [GA]CATG^[CT]
590 ACATGT
PleI 472 GACTC GAGTC(4/5)
256 GAGTC
Sau3AI 505 GATC ^GATC
648 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI AspI
AvaII BanII BbvI BmyI BsaJI
BsaWI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeII MnlI MspI MvaI MwoI
NlaIII NlaIV PflFI PpuMI RsaI
Sau96I ScrFI SfaNI TaqI TseI
TspRI Tth111I TthHB8I