(Circular) (Six-base) MAPSORT of: pFN21AE0512.seq Check: 407 from: 1 to: 5560 pFN21AE0512 5560 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3370 278 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2040 2903 1937 Size: 103 863 4594 Fragments arranged by size: 4594 863 103 Acc65I G'GTAC_C Cuts at: 2881 2881 Size: 5560 AclI AA'CG_TT Cuts at: 1563 3687 4060 1563 Size: 2124 373 3063 Fragments arranged by size: 3063 2124 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3623 5007 5275 1632 Size: 1991 1384 268 1917 Fragments arranged by size: 1991 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5363 AflIII A'CryG_T Cuts at: 1203 2097 2595 4459 5436 1203 Size: 894 498 1864 977 1327 Fragments arranged by size: 1864 1327 977 894 498 AgeI A'CCGG_T Cuts at: 4391 4391 Size: 5560 AhdI GACnn_n'nnGTC Cuts at: 4289 4289 Size: 5560 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5560 AlwNI CAG_nnn'CTG Cuts at: 2704 2722 3303 4875 2704 Size: 18 581 1572 3389 Fragments arranged by size: 3389 1572 581 18 ApaI G_GGCC'C Cuts at: 2024 2024 Size: 5560 ApaLI G'TGCA_C Cuts at: 3617 4773 3617 Size: 1156 4404 ApoI r'AATT_y Cuts at: 1493 2869 3095 3247 5356 1493 Size: 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 226 152 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2531 2885 1870 Size: 73 588 354 4545 Fragments arranged by size: 4545 588 354 73 BamHI G'GATC_C Cuts at: 2509 2890 3257 2509 Size: 381 367 4812 Fragments arranged by size: 4812 381 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2881 4336 618 Size: 325 217 627 1094 1455 1842 Fragments arranged by size: 1842 1455 1094 627 325 217 BanII G_rGCy'C Cuts at: 1391 2024 2367 2409 2532 2559 2826 2879 1391 Size: 633 343 42 123 27 267 53 4072 Fragments arranged by size: 4072 633 343 267 123 53 42 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2116 928 Size: 971 217 4372 Fragments arranged by size: 4372 971 217 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2512 2546 3751 3785 1903 Size: 34 575 34 1205 34 3678 Fragments arranged by size: 3678 1205 575 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3452 4662 3452 Size: 1210 4350 BclI T'GATC_A Cuts at: 1548 1548 Size: 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4171 136 Size: 107 122 71 631 605 2499 1525 Fragments arranged by size: 2499 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5555 5555 Size: 5560 BlpI GC'TnA_GC Cuts at: 2977 2977 Size: 5560 Bme1580I G_kGCm'C Cuts at: 1790 2024 2780 3621 4777 1790 Size: 234 756 841 1156 2573 Fragments arranged by size: 2573 1156 841 756 234 BmgBI CAC'GTC Cuts at: 1318 2100 2794 1318 Size: 782 694 4084 Fragments arranged by size: 4084 782 694 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2182 2404 3291 Size: 416 53 83 399 24 765 222 887 Cuts at: 3291 4249 442 Size: 958 1753 Fragments arranged by size: 1753 958 887 765 416 399 222 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2489 4220 1239 Size: 1250 1731 2579 Fragments arranged by size: 2579 1731 1250 Bpu10I CC'TnA_GC Cuts at: 2852 2852 Size: 5560 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3685 4550 4848 3685 Size: 865 298 4397 Fragments arranged by size: 4397 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4223 882 Size: 617 2724 2219 Fragments arranged by size: 2724 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3351 493 Size: 711 2147 2702 Fragments arranged by size: 2702 2147 711 BsaBI GATnn'nnATC Cuts at: 5554 5554 Size: 5560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3367 3749 4384 275 Size: 53 83 186 936 1834 382 635 1451 Fragments arranged by size: 1834 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2088 2322 2655 3993 4391 4665 4812 5223 2088 Size: 234 333 1338 398 274 147 411 2425 Fragments arranged by size: 2425 1338 411 398 333 274 234 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 1178 Size: 30 165 30 993 30 4312 Fragments arranged by size: 4312 993 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5560 BseYI C'CCAG_C Cuts at: 2856 4763 5259 2856 Size: 1907 496 3157 Fragments arranged by size: 3157 1907 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2728 1163 Size: 573 618 374 3995 Fragments arranged by size: 3995 618 573 374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3010 3771 4799 1540 Size: 275 186 1009 761 1028 2301 Fragments arranged by size: 2301 1028 1009 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2532 3621 3706 4777 2532 Size: 1089 85 1071 3315 Fragments arranged by size: 3315 1089 1071 85 BsmI GAATG_Cn' Cuts at: 3071 3164 3071 Size: 93 5467 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2367 2409 2532 2559 2780 2826 Size: 399 234 343 42 123 27 221 46 Cuts at: 2826 2879 3621 3706 4777 1391 Size: 53 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 234 221 123 85 53 46 42 27 BspEI T'CCGG_A Cuts at: 2088 2322 5223 2088 Size: 234 2901 2425 Fragments arranged by size: 2901 2425 234 BspHI T'CATG_A Cuts at: 3449 5179 3449 Size: 1730 3830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2247 2793 2915 844 Size: 1403 546 122 3489 Fragments arranged by size: 3489 1403 546 122 BsrBI CCG'CTC Cuts at: 1394 1823 3447 1394 Size: 429 1624 3507 Fragments arranged by size: 3507 1624 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4055 4229 65 Size: 1183 2807 174 1396 Fragments arranged by size: 2807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4204 4391 1700 Size: 110 2394 187 2869 Fragments arranged by size: 2869 2394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5560 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5560 BssSI C'ACGA_G Cuts at: 3620 4632 3620 Size: 1012 4548 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5560 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2509 2890 3257 3642 3659 5111 5197 5209 Size: 537 381 367 385 17 1452 86 12 Cuts at: 5209 5555 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 537 385 381 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2637 2637 Size: 5560 BtgI C'CryG_G Cuts at: 513 1065 2232 2463 513 Size: 552 1167 231 3610 Fragments arranged by size: 3610 1167 552 231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4821 Fragments arranged by size: 4821 683 56 BtsI GCAGTG_nn' Cuts at: 2627 3076 3870 3890 2627 Size: 449 794 20 4297 Fragments arranged by size: 4297 794 449 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2864 2864 Size: 5560 DraIII CAC_nnn'GTG Cuts at: 2775 2775 Size: 5560 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4567 809 Size: 507 3251 1802 Fragments arranged by size: 3251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2724 3007 Size: 54 1425 50 275 18 237 657 283 Cuts at: 3007 3896 5525 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 657 283 275 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3007 1537 Size: 275 1195 4090 Fragments arranged by size: 4090 1195 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3490 1353 Size: 2137 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4147 4521 4667 387 Size: 1064 138 2558 374 146 1280 Fragments arranged by size: 2558 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2530 2530 Size: 5560 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2489 3623 4220 5007 5275 1239 Size: 393 857 1134 597 787 268 1524 Fragments arranged by size: 1524 1134 857 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2381 2381 Size: 5560 EcoO109I rG'GnC_Cy Cuts at: 2105 2386 2566 2719 2105 Size: 281 180 153 4946 Fragments arranged by size: 4946 281 180 153 EcoRI G'AATT_C Cuts at: 2869 3247 2869 Size: 378 5182 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 5560 FspI TGC'GCA Cuts at: 4066 4066 Size: 5560 HaeII r_GCGC'y Cuts at: 1678 4707 1678 Size: 3029 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4201 4233 4275 4307 1373 Size: 32 135 32 2629 32 42 32 2626 Fragments arranged by size: 2629 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2904 3150 669 Size: 988 281 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5560 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KpnI G_GTAC'C Cuts at: 2885 2885 Size: 5560 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 2422 4674 4858 1305 Size: 939 178 2252 184 2007 Fragments arranged by size: 2252 2007 939 184 178 MscI TGG'CCA Cuts at: 10 64 1489 1832 2069 2726 10 Size: 54 1425 343 237 657 2844 Fragments arranged by size: 2844 1425 657 343 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3518 3877 4036 518 Size: 648 843 1509 359 159 2042 Fragments arranged by size: 2042 1509 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2312 2704 2976 3653 4801 5046 1606 Size: 706 392 272 677 1148 245 2120 Fragments arranged by size: 2120 1148 706 677 392 272 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5008 NdeI CA'TA_TG Cuts at: 387 387 Size: 5560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5560 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NspI r_CATG'y Cuts at: 1132 1229 2186 2599 2918 4463 1132 Size: 97 957 413 319 1545 2229 Fragments arranged by size: 2229 1545 957 413 319 97 PasI CC'CwG_GG Cuts at: 1348 2736 1348 Size: 1388 4172 PciI A'CATG_T Cuts at: 2595 4459 2595 Size: 1864 3696 PflMI CCAn_nnn'nTGG Cuts at: 1108 2161 1108 Size: 1053 4507 PfoI T'CCnGG_A Cuts at: 2112 2487 2505 2112 Size: 375 18 5167 Fragments arranged by size: 5167 375 18 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3661 5142 5174 3629 Size: 32 1481 32 4015 Fragments arranged by size: 4015 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2105 2386 2566 2719 2105 Size: 281 180 153 4946 Fragments arranged by size: 4946 281 180 153 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 3130 3130 Size: 5560 PspOMI G'GGCC_C Cuts at: 2020 2020 Size: 5560 PstI C_TGCA'G Cuts at: 830 2912 830 Size: 2082 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 PvuII CAG'CTG Cuts at: 2704 2704 Size: 5560 SacI G_AGCT'C Cuts at: 2532 2532 Size: 5560 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SbfI CC_TGCA'GG Cuts at: 2912 2912 Size: 5560 ScaI AGT'ACT Cuts at: 2837 3808 5520 2837 Size: 971 1712 2877 Fragments arranged by size: 2877 1712 971 SexAI A'CCwGG_T Cuts at: 2601 2601 Size: 5560 SfcI C'TryA_G Cuts at: 826 1045 2908 4043 4724 4915 826 Size: 219 1863 1135 681 191 1471 Fragments arranged by size: 1863 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3664 4565 4827 820 Size: 197 926 1721 901 262 1553 Fragments arranged by size: 1721 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5560 SphI G_CATG'C Cuts at: 1132 2186 2918 1132 Size: 1054 732 3774 Fragments arranged by size: 3774 1054 732 SspI AAT'ATT Cuts at: 5 52 3484 5 Size: 47 3432 2081 Fragments arranged by size: 3432 2081 47 StuI AGG'CCT Cuts at: 1517 2459 1517 Size: 942 4618 StyI C'CwwG_G Cuts at: 513 1065 2999 513 Size: 552 1934 3074 Fragments arranged by size: 3074 1934 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2349 2728 3587 3604 3757 3942 4387 Size: 1255 769 379 859 17 153 185 445 Cuts at: 4387 325 Size: 1498 Fragments arranged by size: 1498 1255 859 769 445 379 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2592 2835 3806 Size: 274 80 33 51 1443 615 243 971 Cuts at: 3806 5518 96 Size: 1712 138 Fragments arranged by size: 1712 1443 971 615 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2089 2121 2396 2428 5520 5552 2089 Size: 32 275 32 3092 32 2097 Fragments arranged by size: 3092 2097 275 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2120 2168 2564 1316 Size: 804 48 396 4312 Fragments arranged by size: 4312 804 396 48 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2343 2343 Size: 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 3689 3689 Size: 5560 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3368 276 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI