Restriction map for
pFN21AE0512
(Linear) (Six-base) MAPSORT of: KIEE0512.seq Check: 7102 from: 1 to: 855
KIEE0512.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 0 35 855
Size: 35 820
AflIII A'CryG_T
Cuts at: 0 92 590 855
Size: 92 498 265
Fragments arranged by size:
498 265 92
AlwNI CAG_nnn'CTG
Cuts at: 0 699 717 855
Size: 699 18 138
Fragments arranged by size:
699 138 18
ApaI G_GGCC'C
Cuts at: 0 19 855
Size: 19 836
AvaI C'yCGr_G
Cuts at: 0 526 855
Size: 526 329
BamHI G'GATC_C
Cuts at: 0 504 855
Size: 504 351
BanII G_rGCy'C
Cuts at: 0 19 362 404 527 554 821 855
Size: 19 343 42 123 27 267 34
Fragments arranged by size:
343 267 123 42 34 27 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 111 855
Size: 111 744
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 507 541 855
Size: 507 34 314
Fragments arranged by size:
507 314 34
Bme1580I G_kGCm'C
Cuts at: 0 19 775 855
Size: 19 756 80
Fragments arranged by size:
756 80 19
BmgBI CAC'GTC
Cuts at: 0 95 789 855
Size: 95 694 66
Fragments arranged by size:
694 95 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 399 855
Size: 177 222 456
Fragments arranged by size:
456 222 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 855
Size: 484 371
Bpu10I CC'TnA_GC
Cuts at: 0 847 855
Size: 847 8
BsaWI w'CCGG_w
Cuts at: 0 83 317 650 855
Size: 83 234 333 205
Fragments arranged by size:
333 234 205 83
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 855
Size: 391 30 434
Fragments arranged by size:
434 391 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 723 855
Size: 349 374 132
Fragments arranged by size:
374 349 132
BsiHKAI G_wGCw'C
Cuts at: 0 527 855
Size: 527 328
Bsp1286I G_dGCh'C
Cuts at: 0 19 362 404 527 554 775 821 855
Size: 19 343 42 123 27 221 46 34
Fragments arranged by size:
343 221 123 46 42 34 27 19
BspEI T'CCGG_A
Cuts at: 0 83 317 855
Size: 83 234 538
Fragments arranged by size:
538 234 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 242 788 855
Size: 242 546 67
Fragments arranged by size:
546 242 67
BstYI r'GATC_y
Cuts at: 0 504 855
Size: 504 351
Bsu36I CC'TnA_GG
Cuts at: 0 632 855
Size: 632 223
BtgI C'CryG_G
Cuts at: 0 227 458 855
Size: 227 231 397
Fragments arranged by size:
397 231 227
BtsI GCAGTG_nn'
Cuts at: 0 622 855
Size: 622 233
DraIII CAC_nnn'GTG
Cuts at: 0 770 855
Size: 770 85
EaeI y'GGCC_r
Cuts at: 0 62 719 855
Size: 62 657 136
Fragments arranged by size:
657 136 62
EcoICRI GAG'CTC
Cuts at: 0 525 855
Size: 525 330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 855
Size: 484 371
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 855
Size: 376 479
EcoO109I rG'GnC_Cy
Cuts at: 0 100 381 561 714 855
Size: 100 281 180 153 141
Fragments arranged by size:
281 180 153 141 100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 96 855
Size: 96 759
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 417 855
Size: 239 178 438
Fragments arranged by size:
438 239 178
MscI TGG'CCA
Cuts at: 0 64 721 855
Size: 64 657 134
Fragments arranged by size:
657 134 64
MspA1I CmG'CkG
Cuts at: 0 307 699 855
Size: 307 392 156
Fragments arranged by size:
392 307 156
NspI r_CATG'y
Cuts at: 0 181 594 855
Size: 181 413 261
Fragments arranged by size:
413 261 181
PasI CC'CwG_GG
Cuts at: 0 731 855
Size: 731 124
PciI A'CATG_T
Cuts at: 0 590 855
Size: 590 265
PflMI CCAn_nnn'nTGG
Cuts at: 0 156 855
Size: 156 699
PfoI T'CCnGG_A
Cuts at: 0 107 482 500 855
Size: 107 375 18 355
Fragments arranged by size:
375 355 107 18
PpuMI rG'GwC_Cy
Cuts at: 0 100 381 561 714 855
Size: 100 281 180 153 141
Fragments arranged by size:
281 180 153 141 100
PspOMI G'GGCC_C
Cuts at: 0 15 855
Size: 15 840
PvuII CAG'CTG
Cuts at: 0 699 855
Size: 699 156
SacI G_AGCT'C
Cuts at: 0 527 855
Size: 527 328
ScaI AGT'ACT
Cuts at: 0 832 855
Size: 832 23
SexAI A'CCwGG_T
Cuts at: 0 596 855
Size: 596 259
SphI G_CATG'C
Cuts at: 0 181 855
Size: 181 674
StuI AGG'CCT
Cuts at: 0 454 855
Size: 454 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 344 723 855
Size: 344 379 132
Fragments arranged by size:
379 344 132
TatI w'GTAC_w
Cuts at: 0 587 830 855
Size: 587 243 25
Fragments arranged by size:
587 243 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 84 116 391 423 855
Size: 84 32 275 32 432
Fragments arranged by size:
432 275 84 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 115 163 559 855
Size: 115 48 396 296
Fragments arranged by size:
396 296 115 48
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 338 855
Size: 338 517
Enzymes that do cut:
AccI AflIII AlwNI ApaI AvaI BamHI BanII BbsI
BcgI Bme1580I BmgBI BmrI BpmI Bpu10I BsaWI BsaXI
BsgI BsiHKAI Bsp1286I BspEI BspMI BstYI Bsu36I BtgI
BtsI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I FalI
MmeI MscI MspA1I NspI PasI PciI PflMI PfoI
PpuMI PspOMI PvuII SacI ScaI SexAI SphI StuI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BseRI BseYI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DrdI EagI EarI EciI EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StyI SwaI XbaI XhoI XmaI XmnI ZraI