(Circular) (Six-base) MAPSORT of: pFN21AE0017.seq Check: 9409 from: 1 to: 6355 pFN21AE0017 6355 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3698 1937 Size: 1761 4594 Acc65I G'GTAC_C Cuts at: 3676 3676 Size: 6355 AclI AA'CG_TT Cuts at: 1563 2989 4482 4855 1563 Size: 1426 1493 373 3063 Fragments arranged by size: 3063 1493 1426 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2140 2532 3099 4418 5802 6070 1632 Size: 508 392 567 1319 1384 268 1917 Fragments arranged by size: 1917 1384 1319 567 508 392 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6158 AflIII A'CryG_T Cuts at: 1203 3411 5254 6231 1203 Size: 2208 1843 977 1327 Fragments arranged by size: 2208 1843 1327 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 2341 5084 2341 Size: 2743 3612 AleI CACnn'nnGTG Cuts at: 1166 3239 1166 Size: 2073 4282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3241 1184 Size: 2057 4298 AlwNI CAG_nnn'CTG Cuts at: 2948 3470 4098 5670 2948 Size: 522 628 1572 3633 Fragments arranged by size: 3633 1572 628 522 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 2254 3664 3890 4042 6151 1493 Size: 761 1410 226 152 2109 1697 Fragments arranged by size: 2109 1697 1410 761 226 152 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 3680 1870 Size: 73 1737 4545 Fragments arranged by size: 4545 1737 73 AvrII C'CTAG_G Cuts at: 2426 2426 Size: 6355 BamHI G'GATC_C Cuts at: 3685 4052 3685 Size: 367 5988 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3676 5131 618 Size: 325 217 627 1889 1455 1842 Fragments arranged by size: 1889 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2036 3674 1391 Size: 645 1638 4072 Fragments arranged by size: 4072 1638 645 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2043 2178 2361 2619 928 Size: 971 144 135 183 258 4664 Fragments arranged by size: 4664 971 258 183 144 135 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4546 4580 1903 Size: 34 2609 34 3678 Fragments arranged by size: 3678 2609 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4247 5457 4247 Size: 1210 5145 BclI T'GATC_A Cuts at: 1548 3151 1548 Size: 1603 4752 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3554 4966 136 Size: 107 122 71 631 605 1882 1412 1525 Fragments arranged by size: 1882 1525 1412 631 605 122 107 71 BglII A'GATC_T Cuts at: 6350 6350 Size: 6355 BlpI GC'TnA_GC Cuts at: 3772 3772 Size: 6355 Bme1580I G_kGCm'C Cuts at: 1790 3619 4416 5572 1790 Size: 1829 797 1156 2573 Fragments arranged by size: 2573 1829 1156 797 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6355 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2030 3281 4086 Size: 416 53 83 399 24 613 1251 805 Cuts at: 4086 5044 442 Size: 958 1753 Fragments arranged by size: 1753 1251 958 805 613 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3069 5015 1239 Size: 1830 1946 2579 Fragments arranged by size: 2579 1946 1830 Bpu10I CC'TnA_GC Cuts at: 3138 3138 Size: 6355 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2753 2814 4480 5345 5643 2753 Size: 61 1666 865 298 3465 Fragments arranged by size: 3465 1666 865 298 61 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3427 3590 5018 882 Size: 617 1928 163 1428 2219 Fragments arranged by size: 2219 1928 1428 617 163 BsaAI yAC'GTr Cuts at: 493 1204 4146 493 Size: 711 2942 2702 Fragments arranged by size: 2942 2702 711 BsaBI GATnn'nnATC Cuts at: 2907 6349 2907 Size: 3442 2913 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2387 4162 4544 5179 Size: 53 83 186 936 854 1775 382 635 Cuts at: 5179 275 Size: 1451 Fragments arranged by size: 1775 1451 936 854 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4788 5186 5460 5607 6018 4788 Size: 398 274 147 411 5125 Fragments arranged by size: 5125 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6130 Fragments arranged by size: 6130 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6355 BseYI C'CCAG_C Cuts at: 3338 5558 6054 3338 Size: 2220 496 3639 Fragments arranged by size: 3639 2220 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3436 1163 Size: 573 1700 4082 Fragments arranged by size: 4082 1700 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3805 4566 5594 1540 Size: 275 186 1804 761 1028 2301 Fragments arranged by size: 2301 1804 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4416 4501 5572 4416 Size: 85 1071 5199 Fragments arranged by size: 5199 1071 85 BsmI GAATG_Cn' Cuts at: 2216 2867 2960 3866 3959 2216 Size: 651 93 906 93 4612 Fragments arranged by size: 4612 906 651 93 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 3619 3674 4416 4501 5572 1391 Size: 399 246 1583 55 742 85 1071 2174 Fragments arranged by size: 2174 1583 1071 742 399 246 85 55 BspEI T'CCGG_A Cuts at: 6018 6018 Size: 6355 BspHI T'CATG_A Cuts at: 2062 4244 5974 2062 Size: 2182 1730 2443 Fragments arranged by size: 2443 2182 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3710 844 Size: 2866 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4242 1394 Size: 429 2419 3507 Fragments arranged by size: 3507 2419 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2821 3349 3459 4850 5024 65 Size: 1183 1573 528 110 1391 174 1396 Fragments arranged by size: 1573 1396 1391 1183 528 174 110 BsrFI r'CCGG_y Cuts at: 1700 1810 3132 4999 5186 1700 Size: 110 1322 1867 187 2869 Fragments arranged by size: 2869 1867 1322 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 4415 5427 4415 Size: 1012 5343 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6355 BstBI TT'CG_AA Cuts at: 5159 5159 Size: 6355 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6355 BstXI CCAn_nnnn'nTGG Cuts at: 3047 3047 Size: 6355 BstYI r'GATC_y Cuts at: 1972 3685 4052 4437 4454 5906 5992 6004 6350 Size: 1713 367 385 17 1452 86 12 346 Cuts at: 6350 1972 Size: 1977 Fragments arranged by size: 1977 1713 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3286 513 Size: 552 939 1282 3582 Fragments arranged by size: 3582 1282 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5616 Fragments arranged by size: 5616 683 56 BtsI GCAGTG_nn' Cuts at: 2076 2701 3180 3424 3871 4665 4685 2076 Size: 625 479 244 447 794 20 3746 Fragments arranged by size: 3746 794 625 479 447 244 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6320 DraI TTT'AAA Cuts at: 3659 3659 Size: 6355 DraIII CAC_nnn'GTG Cuts at: 3287 3287 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5362 809 Size: 507 4046 1802 Fragments arranged by size: 4046 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3802 4691 6320 Size: 54 1425 50 275 18 1972 889 1629 Cuts at: 6320 8 Size: 43 Fragments arranged by size: 1972 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3802 1537 Size: 275 1990 4090 Fragments arranged by size: 4090 1990 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 3315 3417 4285 1353 Size: 768 1194 102 868 3423 Fragments arranged by size: 3423 1194 868 768 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4942 5316 5462 387 Size: 1064 138 435 2918 374 146 1280 Fragments arranged by size: 2918 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2532 3069 3099 4418 5015 5802 Size: 393 508 392 537 30 1319 597 787 Cuts at: 5802 6070 1239 Size: 268 1524 Fragments arranged by size: 1524 1319 787 597 537 508 393 392 268 30 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 FspI TGC'GCA Cuts at: 4861 4861 Size: 6355 HaeII r_GCGC'y Cuts at: 1678 5502 1678 Size: 3824 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 2453 2485 4996 Size: 32 135 32 755 32 94 32 2511 Cuts at: 4996 5028 5070 5102 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2511 755 135 94 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3699 3945 669 Size: 988 281 1761 246 3079 Fragments arranged by size: 3079 1761 988 281 246 HindIII A'AGCT_T Cuts at: 748 2869 3090 748 Size: 2121 221 4013 Fragments arranged by size: 4013 2121 221 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KpnI G_GTAC'C Cuts at: 3680 3680 Size: 6355 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5469 5653 1305 Size: 4164 184 2007 Fragments arranged by size: 4164 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4533 Fragments arranged by size: 4533 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2100 3239 4313 4672 4831 518 Size: 648 934 1139 1074 359 159 2042 Fragments arranged by size: 2042 1139 1074 934 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3771 4448 5596 5841 1606 Size: 2165 677 1148 245 2120 Fragments arranged by size: 2165 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4864 Fragments arranged by size: 4864 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6355 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 2454 2882 3415 3713 5258 1132 Size: 97 1225 428 533 298 1545 2229 Fragments arranged by size: 2229 1545 1225 533 428 298 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6355 PciI A'CATG_T Cuts at: 3411 5254 3411 Size: 1843 4512 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6355 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2182 2214 2868 2900 4424 4456 5937 5969 2182 Size: 32 654 32 1524 32 1481 32 2568 Fragments arranged by size: 2568 1524 1481 654 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6355 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PstI C_TGCA'G Cuts at: 830 2725 3707 830 Size: 1895 982 3478 Fragments arranged by size: 3478 1895 982 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SbfI CC_TGCA'GG Cuts at: 3707 3707 Size: 6355 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SfcI C'TryA_G Cuts at: 826 1045 2721 3703 4838 5519 5710 826 Size: 219 1676 982 1135 681 191 1471 Fragments arranged by size: 1676 1471 1135 982 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3682 1872 Size: 1810 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2768 2829 4459 5360 5622 820 Size: 197 926 825 61 1630 901 262 1553 Fragments arranged by size: 1630 1553 926 901 825 262 197 61 SnaBI TAC'GTA Cuts at: 493 4146 493 Size: 3653 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 3713 1132 Size: 2581 3774 SspI AAT'ATT Cuts at: 5 52 4279 5 Size: 47 4227 2081 Fragments arranged by size: 4227 2081 47 StuI AGG'CCT Cuts at: 1517 2431 2825 1517 Size: 914 394 5047 Fragments arranged by size: 5047 914 394 StyI C'CwwG_G Cuts at: 513 1065 2004 2426 3794 513 Size: 552 939 422 1368 3074 Fragments arranged by size: 3074 1368 939 552 422 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3086 3497 4382 4399 4552 4737 5182 Size: 1255 1506 411 885 17 153 185 445 Cuts at: 5182 325 Size: 1498 Fragments arranged by size: 1506 1498 1255 885 445 411 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4601 6313 96 Size: 274 80 33 51 1443 2624 1712 138 Fragments arranged by size: 2624 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2680 2712 6315 6347 2680 Size: 32 3603 32 2688 Fragments arranged by size: 3603 2688 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6355 XbaI T'CTAG_A Cuts at: 2200 3691 2200 Size: 1491 4864 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3550 3550 Size: 6355 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6355 XmaI C'CCGG_G Cuts at: 1870 3680 1870 Size: 1810 4545 XmnI GAAnn'nnTTC Cuts at: 4484 4484 Size: 6355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI