(Circular) (Six-base) MAPSORT of: pFN21AB8622.seq Check: 4503 from: 1 to: 7222 pFN21AB8622 7222 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3018 3018 Size: 7222 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2427 3390 5032 278 Size: 53 83 186 936 891 963 1642 2468 Fragments arranged by size: 2468 1642 963 936 891 186 83 53 AccI GT'mk_AC Cuts at: 1937 2427 4565 1937 Size: 490 2138 4594 Fragments arranged by size: 4594 2138 490 Acc65I G'GTAC_C Cuts at: 4543 4543 Size: 7222 AclI AA'CG_TT Cuts at: 1563 4288 5349 5722 1563 Size: 2725 1061 373 3063 Fragments arranged by size: 3063 2725 1061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2592 2893 2957 4250 5285 6669 6937 1632 Size: 960 301 64 1293 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1293 1035 960 301 268 64 AfeI AGC'GCT Cuts at: 4091 4091 Size: 7222 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7025 AflIII A'CryG_T Cuts at: 1203 2766 3561 3630 6121 7098 1203 Size: 1563 795 69 2491 977 1327 Fragments arranged by size: 2491 1563 1327 977 795 69 AgeI A'CCGG_T Cuts at: 6053 6053 Size: 7222 AhdI GACnn_n'nnGTC Cuts at: 3847 5951 3847 Size: 2104 5118 AleI CACnn'nnGTG Cuts at: 1166 4349 1166 Size: 3183 4039 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2069 1184 Size: 885 6337 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3213 3245 3213 Size: 32 7190 AlwNI CAG_nnn'CTG Cuts at: 2113 2143 2212 2890 4965 6537 2113 Size: 30 69 678 2075 1572 2798 Fragments arranged by size: 2798 2075 1572 678 69 30 ApaLI G'TGCA_C Cuts at: 2929 5279 6435 2929 Size: 2350 1156 3716 Fragments arranged by size: 3716 2350 1156 ApoI r'AATT_y Cuts at: 1493 2258 2954 3152 3172 3194 4181 4531 4757 Size: 765 696 198 20 22 987 350 226 Cuts at: 4757 4909 7018 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 987 765 696 350 226 198 152 22 20 AseI AT'TA_AT Cuts at: 160 1032 5776 160 Size: 872 4744 1606 Fragments arranged by size: 4744 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 AvaI C'yCGr_G Cuts at: 1870 1943 3785 3963 4281 4374 4547 1870 Size: 73 1842 178 318 93 173 4545 Fragments arranged by size: 4545 1842 318 178 173 93 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3619 3652 4250 4283 3619 Size: 33 598 33 6558 Fragments arranged by size: 6558 598 33 33 BamHI G'GATC_C Cuts at: 4158 4552 4919 4158 Size: 394 367 6461 Fragments arranged by size: 6461 394 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3611 4543 5998 618 Size: 325 217 627 1824 932 1455 1842 Fragments arranged by size: 1842 1824 1455 932 627 325 217 BanII G_rGCy'C Cuts at: 1391 2669 2690 3327 4541 1391 Size: 1278 21 637 1214 4072 Fragments arranged by size: 4072 1278 1214 637 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2086 2701 2797 3208 928 Size: 971 187 615 96 411 4942 Fragments arranged by size: 4942 971 615 411 187 96 BbvCI CC'TCA_GC Cuts at: 3833 3833 Size: 7222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3962 3996 5413 5447 1903 Size: 34 2025 34 1417 34 3678 Fragments arranged by size: 3678 2025 1417 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 5114 6324 3620 Size: 1494 1210 4518 Fragments arranged by size: 4518 1494 1210 BclI T'GATC_A Cuts at: 1548 2730 4062 1548 Size: 1182 1332 4708 Fragments arranged by size: 4708 1332 1182 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3055 4301 4434 Size: 107 122 71 631 605 1383 1246 133 Cuts at: 4434 5833 136 Size: 1399 1525 Fragments arranged by size: 1525 1399 1383 1246 631 605 133 122 107 71 BglII A'GATC_T Cuts at: 3709 7217 3709 Size: 3508 3714 BlpI GC'TnA_GC Cuts at: 2684 4086 4639 2684 Size: 1402 553 5267 Fragments arranged by size: 5267 1402 553 Bme1580I G_kGCm'C Cuts at: 1790 2933 3614 3658 5283 6439 1790 Size: 1143 681 44 1625 1156 2573 Fragments arranged by size: 2573 1625 1156 1143 681 44 BmgBI CAC'GTC Cuts at: 1318 2767 3820 4411 1318 Size: 1449 1053 591 4129 Fragments arranged by size: 4129 1449 1053 591 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2200 4313 4953 Size: 416 53 83 399 24 783 2113 640 Cuts at: 4953 5911 442 Size: 958 1753 Fragments arranged by size: 2113 1753 958 783 640 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2459 2621 2660 2741 3209 4217 4282 4291 Size: 1220 162 39 81 468 1008 65 9 Cuts at: 4291 5882 1239 Size: 1591 2579 Fragments arranged by size: 2579 1591 1220 1008 468 162 81 65 39 9 Bpu10I CC'TnA_GC Cuts at: 3833 3833 Size: 7222 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2159 3157 3311 3845 4019 4247 5347 6212 6510 Size: 998 154 534 174 228 1100 865 298 Cuts at: 6510 2159 Size: 2871 Fragments arranged by size: 2871 1100 998 865 534 298 228 174 154 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2396 2606 3125 4434 4457 5885 882 Size: 617 897 210 519 1309 23 1428 2219 Fragments arranged by size: 2219 1428 1309 897 617 519 210 23 BsaAI yAC'GTr Cuts at: 493 1204 4501 5013 493 Size: 711 3297 512 2702 Fragments arranged by size: 3297 2702 711 512 BsaBI GATnn'nnATC Cuts at: 3104 4163 7216 3104 Size: 1059 3053 3110 Fragments arranged by size: 3110 3053 1059 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2424 3387 3648 3717 Size: 53 83 186 936 891 963 261 69 Cuts at: 3717 5029 5411 6046 275 Size: 1312 382 635 1451 Fragments arranged by size: 1451 1312 963 936 891 635 382 261 186 83 69 53 BsaWI w'CCGG_w Cuts at: 3231 3722 5655 6053 6327 6474 6885 3231 Size: 491 1933 398 274 147 411 3568 Fragments arranged by size: 3568 1933 491 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2138 2168 1178 Size: 30 165 30 735 30 6232 Fragments arranged by size: 6232 735 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 2456 2939 4523 1186 Size: 1228 42 483 1584 3885 Fragments arranged by size: 3885 1584 1228 483 42 BseYI C'CCAG_C Cuts at: 3775 3902 6425 6921 3775 Size: 127 2523 496 4076 Fragments arranged by size: 4076 2523 496 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2129 2339 2564 1163 Size: 573 393 210 225 5821 Fragments arranged by size: 5821 573 393 225 210 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2029 2088 2195 3956 4672 5433 Size: 275 186 28 59 107 1761 716 761 Cuts at: 5433 6461 1540 Size: 1028 2301 Fragments arranged by size: 2301 1761 1028 761 716 275 186 107 59 28 BsiHKAI G_wGCw'C Cuts at: 2865 2933 3000 3327 5283 5368 6439 2865 Size: 68 67 327 1956 85 1071 3648 Fragments arranged by size: 3648 1956 1071 327 85 68 67 BsiWI C'GTAC_G Cuts at: 4497 4497 Size: 7222 BsmI GAATG_Cn' Cuts at: 4733 4826 4733 Size: 93 7129 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2669 2690 2865 2933 3000 3327 3614 Size: 399 879 21 175 68 67 327 287 Cuts at: 3614 3658 4541 5283 5368 6439 1391 Size: 44 883 742 85 1071 2174 Fragments arranged by size: 2174 1071 883 879 742 399 327 287 175 85 68 67 44 21 BspEI T'CCGG_A Cuts at: 3722 6885 3722 Size: 3163 4059 BspHI T'CATG_A Cuts at: 5111 6841 5111 Size: 1730 5492 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2979 3018 3989 4038 4577 844 Size: 2135 39 971 49 539 3489 Fragments arranged by size: 3489 2135 971 539 49 39 BsrBI CCG'CTC Cuts at: 1394 1823 3728 4072 5109 1394 Size: 429 1905 344 1037 3507 Fragments arranged by size: 3507 1905 1037 429 344 BsrDI GCAATG_nn' Cuts at: 65 1248 5717 5891 65 Size: 1183 4469 174 1396 Fragments arranged by size: 4469 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3951 4311 5866 6053 1700 Size: 110 2141 360 1555 187 2869 Fragments arranged by size: 2869 2141 1555 360 187 110 BsrGI T'GTAC_A Cuts at: 96 3102 3558 96 Size: 3006 456 3760 Fragments arranged by size: 3760 3006 456 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7222 BssSI C'ACGA_G Cuts at: 5282 6294 5282 Size: 1012 6210 BstAPI GCAn_nnn'nTGC Cuts at: 794 2113 2971 2995 3591 794 Size: 1319 858 24 596 4425 Fragments arranged by size: 4425 1319 858 596 24 BstBI TT'CG_AA Cuts at: 6026 6026 Size: 7222 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7222 BstXI CCAn_nnnn'nTGG Cuts at: 2560 3748 2560 Size: 1188 6034 BstYI r'GATC_y Cuts at: 1972 2604 3709 4158 4419 4552 4919 5304 5321 Size: 632 1105 449 261 133 367 385 17 Cuts at: 5321 6773 6859 6871 7217 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1105 632 449 385 367 346 261 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2753 3605 2753 Size: 852 6370 BtgI C'CryG_G Cuts at: 513 1065 2004 2088 2334 3766 3854 513 Size: 552 939 84 246 1432 88 3881 Fragments arranged by size: 3881 1432 939 552 246 88 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2347 3113 487 Size: 683 56 1121 766 4596 Fragments arranged by size: 4596 1121 766 683 56 BtsI GCAGTG_nn' Cuts at: 2018 3978 4738 5532 5552 2018 Size: 1960 760 794 20 3688 Fragments arranged by size: 3688 1960 794 760 20 ClaI AT'CG_AT Cuts at: 1554 2040 1554 Size: 486 6736 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7187 DraI TTT'AAA Cuts at: 4526 4526 Size: 7222 DraIII CAC_nnn'GTG Cuts at: 2071 2071 Size: 7222 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2423 3287 6229 809 Size: 507 1107 864 2942 1802 Fragments arranged by size: 2942 1802 1107 864 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2085 2192 Size: 54 1425 50 275 18 196 59 107 Cuts at: 2192 2448 2796 2892 3501 3750 3953 4353 4669 Size: 256 348 96 609 249 203 400 316 Cuts at: 4669 5558 7187 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 609 400 348 316 275 256 249 203 196 107 96 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2026 2085 2192 3953 4669 1537 Size: 275 214 59 107 1761 716 4090 Fragments arranged by size: 4090 1761 716 275 214 107 59 EarI CTCTTCn'nnn_ Cuts at: 1353 2306 3467 4500 5152 1353 Size: 953 1161 1033 652 3423 Fragments arranged by size: 3423 1161 1033 953 652 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2708 4452 5809 6183 6329 387 Size: 1064 138 1119 1744 1357 374 146 1280 Fragments arranged by size: 1744 1357 1280 1119 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3325 3325 Size: 7222 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2459 2592 2621 2660 2741 2893 2957 Size: 393 827 133 29 39 81 152 64 Cuts at: 2957 3209 4217 4250 4282 4291 5285 5882 6669 Size: 252 1008 33 32 9 994 597 787 Cuts at: 6669 6937 1239 Size: 268 1524 Fragments arranged by size: 1524 1008 994 827 787 597 393 268 252 152 133 81 64 39 33 32 29 9 EcoNI CCTnn'n_nnAGG Cuts at: 2105 2468 2732 2105 Size: 363 264 6595 Fragments arranged by size: 6595 363 264 EcoO109I rG'GnC_Cy Cuts at: 2050 2125 2725 3737 4053 4302 2050 Size: 75 600 1012 316 249 4970 Fragments arranged by size: 4970 1012 600 316 249 75 EcoRI G'AATT_C Cuts at: 4531 4909 4531 Size: 378 6844 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3151 3457 4255 3151 Size: 306 798 6118 Fragments arranged by size: 6118 798 306 FspI TGC'GCA Cuts at: 5728 5728 Size: 7222 HaeII r_GCGC'y Cuts at: 1678 2114 4093 6369 1678 Size: 436 1979 2276 2531 Fragments arranged by size: 2531 2276 1979 436 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3833 3865 5863 5895 5937 Size: 32 135 32 2261 32 1998 32 42 Cuts at: 5937 5969 1373 Size: 32 2626 Fragments arranged by size: 2626 2261 1998 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3244 4566 4812 669 Size: 988 281 1306 1322 246 3079 Fragments arranged by size: 3079 1322 1306 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7222 HpaI GTT'AAC Cuts at: 4812 4812 Size: 7222 KpnI G_GTAC'C Cuts at: 4547 4547 Size: 7222 MfeI C'AATT_G Cuts at: 4821 4821 Size: 7222 MluI A'CGCG_T Cuts at: 7098 7098 Size: 7222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6336 6520 1305 Size: 5031 184 2007 Fragments arranged by size: 5031 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2798 2894 3503 3752 4355 Size: 54 1425 343 966 96 609 249 603 Cuts at: 4355 10 Size: 2877 Fragments arranged by size: 2877 1425 966 609 603 343 249 96 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2558 2765 4136 4349 5180 5539 Size: 648 1368 24 207 1371 213 831 359 Cuts at: 5539 5698 518 Size: 159 2042 Fragments arranged by size: 2042 1371 1368 831 648 359 213 207 159 24 MspA1I CmG'CkG Cuts at: 1606 2031 2140 2336 2705 4050 4249 4349 4638 Size: 425 109 196 369 1345 199 100 289 Cuts at: 4638 5315 6463 6708 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1345 1148 677 425 369 289 245 199 196 109 100 NaeI GCC'GGC Cuts at: 3953 3953 Size: 7222 NcoI C'CATG_G Cuts at: 513 1065 2004 3766 513 Size: 552 939 1762 3969 Fragments arranged by size: 3969 1762 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7222 NgoMIV G'CCGG_C Cuts at: 3951 3951 Size: 7222 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7222 NotI GC'GGCC_GC Cuts at: 2026 4669 2026 Size: 2643 4579 NspI r_CATG'y Cuts at: 1132 1229 3634 4580 6125 1132 Size: 97 2405 946 1545 2229 Fragments arranged by size: 2405 2229 1545 946 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7222 PciI A'CATG_T Cuts at: 3630 6121 3630 Size: 2491 4731 PflMI CCAn_nnn'nTGG Cuts at: 1108 2071 2890 1108 Size: 963 819 5440 Fragments arranged by size: 5440 963 819 PfoI T'CCnGG_A Cuts at: 2607 2943 4194 2607 Size: 336 1251 5635 Fragments arranged by size: 5635 1251 336 PmeI GTTT'AAAC Cuts at: 4526 4526 Size: 7222 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3332 3364 5291 5323 6804 6836 3332 Size: 32 1927 32 1481 32 3718 Fragments arranged by size: 3718 1927 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2050 2125 2725 3737 4053 2050 Size: 75 600 1012 316 5219 Fragments arranged by size: 5219 1012 600 316 75 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7222 PsiI TTA'TAA Cuts at: 4792 4792 Size: 7222 PstI C_TGCA'G Cuts at: 830 2852 3709 4574 830 Size: 2022 857 865 3478 Fragments arranged by size: 3478 2022 865 857 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7222 PvuII CAG'CTG Cuts at: 2140 2705 4249 4349 2140 Size: 565 1544 100 5013 Fragments arranged by size: 5013 1544 565 100 SacI G_AGCT'C Cuts at: 3327 3327 Size: 7222 SacII CC_GC'GG Cuts at: 2337 2337 Size: 7222 SalI G'TCGA_C Cuts at: 1936 4564 1936 Size: 2628 4594 SbfI CC_TGCA'GG Cuts at: 4574 4574 Size: 7222 ScaI AGT'ACT Cuts at: 2627 2846 4211 5470 7182 2627 Size: 219 1365 1259 1712 2667 Fragments arranged by size: 2667 1712 1365 1259 219 SexAI A'CCwGG_T Cuts at: 3733 3733 Size: 7222 SfcI C'TryA_G Cuts at: 826 1045 2848 3165 3313 3705 4423 4570 5705 Size: 219 1803 317 148 392 718 147 1135 Cuts at: 5705 6386 6577 826 Size: 681 191 1471 Fragments arranged by size: 1803 1471 1135 718 681 392 317 219 191 148 147 SfiI GGCCn_nnn'nGGCC Cuts at: 4301 4301 Size: 7222 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7222 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7222 SmaI CCC'GGG Cuts at: 1872 3787 3965 4283 4549 1872 Size: 1915 178 318 266 4545 Fragments arranged by size: 4545 1915 318 266 178 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 3136 3326 3824 4034 4262 Size: 197 926 231 962 190 498 210 228 Cuts at: 4262 5326 6227 6489 820 Size: 1064 901 262 1553 Fragments arranged by size: 1553 1064 962 926 901 498 262 231 228 210 197 190 SnaBI TAC'GTA Cuts at: 493 4501 5013 493 Size: 4008 512 2702 Fragments arranged by size: 4008 2702 512 SpeI A'CTAG_T Cuts at: 152 152 Size: 7222 SphI G_CATG'C Cuts at: 1132 4580 1132 Size: 3448 3774 SspI AAT'ATT Cuts at: 5 52 5146 5 Size: 47 5094 2081 Fragments arranged by size: 5094 2081 47 StuI AGG'CCT Cuts at: 1517 2386 1517 Size: 869 6353 StyI C'CwwG_G Cuts at: 513 1065 2004 2799 3766 4661 513 Size: 552 939 795 967 895 3074 Fragments arranged by size: 3074 967 939 895 795 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2630 3377 5249 5266 5419 5604 6049 Size: 1255 1050 747 1872 17 153 185 445 Cuts at: 6049 325 Size: 1498 Fragments arranged by size: 1872 1498 1255 1050 747 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2625 2844 3102 Size: 274 80 33 51 1443 648 219 258 Cuts at: 3102 3558 4209 5468 7180 96 Size: 456 651 1259 1712 138 Fragments arranged by size: 1712 1443 1259 651 648 456 274 258 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2138 2170 2925 2957 7182 7214 2138 Size: 32 755 32 4225 32 2146 Fragments arranged by size: 4225 2146 755 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2123 2486 3735 1316 Size: 807 363 1249 4803 Fragments arranged by size: 4803 1249 807 363 XbaI T'CTAG_A Cuts at: 4558 4558 Size: 7222 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2258 3091 2068 Size: 190 833 6199 Fragments arranged by size: 6199 833 190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7222 XmaI C'CCGG_G Cuts at: 1870 3785 3963 4281 4547 1870 Size: 1915 178 318 266 4545 Fragments arranged by size: 4545 1915 318 266 178 XmnI GAAnn'nnTTC Cuts at: 2826 5351 2826 Size: 2525 4697 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2425 3388 5030 276 Size: 53 83 186 936 891 963 1642 2468 Fragments arranged by size: 2468 1642 963 936 891 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BbeI BfrBI BplI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfoI SrfI SwaI