Restriction map for
pFN21AE3580
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# NO CUT ENZYMES ( 90 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AvrII BbsI BbvCI
BciVI BclI BfrI BlnI BpuAI
BseAI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I DraI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI MfeI MluI MroI MseI
MunI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 1798 GACGTC GACGT^C
Acc65I 748 GGTACC G^GTACC
Aor51HI 2504 AGCGCT AGC^GCT
Asp718I 748 GGTACC G^GTACC
AviII 2120 TGCGCA TGC^GCA
BalI 725 TGGCCA TGG^CCA
BamHI 584 GGATCC G^GATCC
BglII 2987 AGATCT A^GATCT
BlpI 2940 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2940 GCTCAGC GC^T[ACGT]AGC
BsaBI 2085 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 532 GAGACC GGTCTC(1/5)
BsaWI 2126 ACCGGA [AT]^CCGG[AT]
BspDI 1903 ATCGAT AT^CGAT
CelII 2940 GCTCAGC GC^T[ACGT]AGC
ClaI 1903 ATCGAT AT^CGAT
CpoI 1031 CGGACCG CG^G[AT]CCG
DrdI 934 GACACTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 100 CGGCCG C^GGCCG
EarI 1410 GAAGAG CTCTTC(1/4)
EclXI 100 CGGCCG C^GGCCG
Eco47III 2504 AGCGCT AGC^GCT
Eco52I 100 CGGCCG C^GGCCG
FspI 2120 TGCGCA TGC^GCA
HindIII 1396 AAGCTT A^AGCTT
KpnI 748 GGTACC GGTAC^C
Ksp632I 1410 GAAGAG CTCTTC(1/4)
MamI 2085 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 725 TGGCCA TGG^CCA
MscI 725 TGGCCA TGG^CCA
NheI 1971 GCTAGC G^CTAGC
PflMI 2808 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 561 GAGTC GAGTC(4/5)
RsrII 1031 CGGACCG CG^G[AT]CCG
SacI 505 GAGCTC GAGCT^C
StuI 2339 AGGCCT AGG^CCT
TfiI 1986 GATTC G^A[AT]TC
Van91I 2808 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AspI 913 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1126 GACGTGGTC
BfaI 23 CTAG C^TAG
1972 CTAG
BsmBI 1066 GAGACG CGTCTC(1/5)
1247 GAGACG
BssSI 1063 CACGAG CACGAG(-5/-1)
2893 CTCGTG
DraIII 477 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2682 CACCGTGTG
EcoNI 1554 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1884 CCTCACGGAGG
EcoT14I 844 CCAAGG C^C[AT][AT]GG
1521 CCAAGG
HincII 1801 GTCAAC GT[CT]^[GA]AC
2212 GTCAAC
HindII 1801 GTCAAC GT[CT]^[GA]AC
2212 GTCAAC
HinfI 561 GAGTC G^A[ACGT]TC
1986 GATTC
KspI 1035 CCGCGG CCGC^GG
2436 CCGCGG
MaeI 23 CTAG C^TAG
1972 CTAG
PflFI 913 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1126 GACGTGGTC
SacII 1035 CCGCGG CCGC^GG
2436 CCGCGG
SphI 167 GCATGC GCATG^C
2917 GCATGC
StyI 844 CCAAGG C^C[AT][AT]GG
1521 CCAAGG
Tth111I 913 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1126 GACGTGGTC
XcmI 1334 CCAACCTGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1592 CCAGGGCGGGCGTGG
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbrPI
BbvI BcgI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI TaqI
TseI Tsp45I TspRI TthHB8I XhoII
XmaCI XmaI