(Circular) (Six-base) MAPSORT of: pFN21AE3580.seq Check: 9255 from: 1 to: 7702 pFN21AE3580 7702 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2155 3499 4874 2155 Size: 1344 1375 4983 Fragments arranged by size: 4983 1375 1344 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3807 5512 278 Size: 53 83 186 936 2271 1705 2468 Fragments arranged by size: 2468 2271 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5045 1937 Size: 3108 4594 Acc65I G'GTAC_C Cuts at: 2753 5023 2753 Size: 2270 5432 AclI AA'CG_TT Cuts at: 1563 5829 6202 1563 Size: 4266 373 3063 Fragments arranged by size: 4266 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3280 4478 5765 7149 7417 1632 Size: 1648 1198 1287 1384 268 1917 Fragments arranged by size: 1917 1648 1384 1287 1198 268 AfeI AGC'GCT Cuts at: 4511 4511 Size: 7702 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7505 AflIII A'CryG_T Cuts at: 1203 6601 7578 1203 Size: 5398 977 1327 Fragments arranged by size: 5398 1327 977 AgeI A'CCGG_T Cuts at: 6533 6533 Size: 7702 AhdI GACnn_n'nnGTC Cuts at: 6431 6431 Size: 7702 AleI CACnn'nnGTG Cuts at: 1166 2243 2762 3680 3896 1166 Size: 1077 519 918 216 4972 Fragments arranged by size: 4972 1077 918 519 216 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3567 3755 4074 4772 1184 Size: 2383 188 319 698 4114 Fragments arranged by size: 4114 2383 698 319 188 AlwNI CAG_nnn'CTG Cuts at: 3278 4279 4330 4953 5445 7017 3278 Size: 1001 51 623 492 1572 3963 Fragments arranged by size: 3963 1572 1001 623 492 51 ApaI G_GGCC'C Cuts at: 3412 3784 4320 4553 4617 3412 Size: 372 536 233 64 6497 Fragments arranged by size: 6497 536 372 233 64 ApaLI G'TGCA_C Cuts at: 5759 6915 5759 Size: 1156 6546 ApoI r'AATT_y Cuts at: 1493 5011 5237 5389 7498 1493 Size: 3518 226 152 2109 1697 Fragments arranged by size: 3518 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6256 160 Size: 872 5224 1606 Fragments arranged by size: 5224 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7702 AvaI C'yCGr_G Cuts at: 1870 1943 2118 2584 3092 3326 3363 3433 3474 Size: 73 175 466 508 234 37 70 41 Cuts at: 3474 3668 3999 4348 4546 4565 4616 5027 1870 Size: 194 331 349 198 19 51 411 4545 Fragments arranged by size: 4545 508 466 411 349 331 234 198 194 175 73 70 51 41 37 19 BamHI G'GATC_C Cuts at: 2589 5032 5399 2589 Size: 2443 367 4892 Fragments arranged by size: 4892 2443 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2391 2753 3017 3498 3699 Size: 325 217 627 604 362 264 481 201 Cuts at: 3699 3898 4186 4415 4601 4731 5023 6478 618 Size: 199 288 229 186 130 292 1455 1842 Fragments arranged by size: 1842 1455 627 604 481 362 325 292 288 264 229 217 201 199 186 130 BanII G_rGCy'C Cuts at: 1391 2514 2654 3412 3481 3784 4320 4454 4553 Size: 1123 140 758 69 303 536 134 99 Cuts at: 4553 4617 5021 1391 Size: 64 404 4072 Fragments arranged by size: 4072 1123 758 536 404 303 140 134 99 69 64 BbeI G_GCGC'C Cuts at: 3502 4419 4605 3502 Size: 917 186 6599 Fragments arranged by size: 6599 917 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6731 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2275 2309 2608 2642 3317 3351 5893 Size: 34 338 34 299 34 675 34 2542 Cuts at: 5893 5927 1903 Size: 34 3678 Fragments arranged by size: 3678 2542 675 338 299 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5594 6804 5594 Size: 1210 6492 BclI T'GATC_A Cuts at: 1548 1548 Size: 7702 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 2262 2976 Size: 107 122 71 631 605 421 169 714 Cuts at: 2976 3854 4359 4767 6313 136 Size: 878 505 408 1546 1525 Fragments arranged by size: 1546 1525 878 714 631 605 505 421 408 169 122 107 71 BglII A'GATC_T Cuts at: 4992 7697 4992 Size: 2705 4997 BlpI GC'TnA_GC Cuts at: 4946 5119 4946 Size: 173 7529 Bme1580I G_kGCm'C Cuts at: 1790 2240 2315 3020 3412 3784 3863 4320 4553 Size: 450 75 705 392 372 79 457 233 Cuts at: 4553 4617 5763 6919 1790 Size: 64 1146 1156 2573 Fragments arranged by size: 2573 1156 1146 705 457 450 392 372 233 79 75 64 BmgBI CAC'GTC Cuts at: 1318 2742 3049 3133 3349 4117 4423 1318 Size: 1424 307 84 216 768 306 4597 Fragments arranged by size: 4597 1424 768 307 306 216 84 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2650 5433 6391 Size: 416 53 83 399 24 1233 2783 958 Cuts at: 6391 442 Size: 1753 Fragments arranged by size: 2783 1753 1233 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3980 1056 Size: 2924 4778 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2668 2668 Size: 7702 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3788 3885 4430 6362 1239 Size: 2549 97 545 1932 2579 Fragments arranged by size: 2579 2549 1932 545 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2063 2783 3947 4469 5827 6692 6990 2063 Size: 720 1164 522 1358 865 298 2775 Fragments arranged by size: 2775 1358 1164 865 720 522 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2531 6365 882 Size: 617 1032 3834 2219 Fragments arranged by size: 3834 2219 1032 617 BsaAI yAC'GTr Cuts at: 493 1204 3745 4177 4255 4597 5493 493 Size: 711 2541 432 78 342 896 2702 Fragments arranged by size: 2702 2541 896 711 432 342 78 BsaBI GATnn'nnATC Cuts at: 4094 7696 4094 Size: 3602 4100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2385 3499 3711 3804 Size: 53 83 186 936 852 1114 212 93 Cuts at: 3804 3819 4020 4416 4602 5509 5891 6526 275 Size: 15 201 396 186 907 382 635 1451 Fragments arranged by size: 1451 1114 936 907 852 635 396 382 212 201 186 186 93 83 53 15 BsaWI w'CCGG_w Cuts at: 4131 6135 6533 6807 6954 7365 4131 Size: 2004 398 274 147 411 4468 Fragments arranged by size: 4468 2004 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2607 2637 3941 3971 1178 Size: 30 165 30 1204 30 1304 30 4909 Fragments arranged by size: 4909 1304 1204 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3344 4247 4712 4838 1186 Size: 2158 903 465 126 4050 Fragments arranged by size: 4050 2158 903 465 126 BseYI C'CCAG_C Cuts at: 2018 2703 3179 3460 3695 4205 4506 6905 7401 Size: 685 476 281 235 510 301 2399 496 Cuts at: 7401 2018 Size: 2319 Fragments arranged by size: 2399 2319 685 510 496 476 301 281 235 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 2318 2464 3077 3626 3725 3773 Size: 573 447 135 146 613 549 99 48 Cuts at: 3773 3824 3932 4231 4235 4577 1163 Size: 51 108 299 4 342 4288 Fragments arranged by size: 4288 613 573 549 447 342 299 146 135 108 99 51 48 4 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2108 2284 2530 4392 4447 4984 Size: 275 186 107 176 246 1862 55 537 Cuts at: 4984 5152 5913 6941 1540 Size: 168 761 1028 2301 Fragments arranged by size: 2301 1862 1028 761 537 275 246 186 176 168 107 55 BsiHKAI G_wGCw'C Cuts at: 2514 3432 3687 3734 4485 4643 5763 5848 6919 Size: 918 255 47 751 158 1120 85 1071 Cuts at: 6919 2514 Size: 3297 Fragments arranged by size: 3297 1120 1071 918 751 255 158 85 47 BsmI GAATG_Cn' Cuts at: 5213 5306 5213 Size: 93 7609 BsmBI CGTCTCn'nnnn_ Cuts at: 3065 3246 3065 Size: 181 7521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2315 2514 2654 3020 3412 3432 Size: 399 450 75 199 140 366 392 20 Cuts at: 3432 3481 3687 3734 3784 3863 4320 4454 4485 Size: 49 206 47 50 79 457 134 31 Cuts at: 4485 4553 4617 4643 5021 5763 5848 6919 1391 Size: 68 64 26 378 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 450 399 392 378 366 206 199 140 134 85 79 75 68 64 50 49 47 31 26 20 BspEI T'CCGG_A Cuts at: 7365 7365 Size: 7702 BspHI T'CATG_A Cuts at: 5591 7321 5591 Size: 1730 5972 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2155 2364 3270 3499 3621 3819 4599 4874 Size: 1311 209 906 229 122 198 780 275 Cuts at: 4874 5057 844 Size: 183 3489 Fragments arranged by size: 3489 1311 906 780 275 229 209 198 183 122 BsrBI CCG'CTC Cuts at: 1394 1823 2543 3539 4522 5589 1394 Size: 429 720 996 983 1067 3507 Fragments arranged by size: 3507 1067 996 983 720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6197 6371 65 Size: 1183 4949 174 1396 Fragments arranged by size: 4949 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2333 2863 3321 3869 4842 6346 6533 Size: 110 523 530 458 548 973 1504 187 Cuts at: 6533 1700 Size: 2869 Fragments arranged by size: 2869 1504 973 548 530 523 458 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7702 BssHII G'CGCG_C Cuts at: 1925 2346 3358 4704 4706 1925 Size: 421 1012 1346 2 4921 Fragments arranged by size: 4921 1346 1012 421 2 BssSI C'ACGA_G Cuts at: 3068 4898 5762 6774 3068 Size: 1830 864 1012 3996 Fragments arranged by size: 3996 1830 1012 864 BstAPI GCAn_nnn'nTGC Cuts at: 794 2356 2431 2487 4731 4866 794 Size: 1562 75 56 2244 135 3630 Fragments arranged by size: 3630 2244 1562 135 75 56 BstBI TT'CG_AA Cuts at: 6506 6506 Size: 7702 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7702 BstYI r'GATC_y Cuts at: 1972 2589 4089 4992 5032 5399 5784 5801 7253 Size: 617 1500 903 40 367 385 17 1452 Cuts at: 7253 7339 7351 7697 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1500 1452 903 617 385 367 346 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2260 2698 2971 3040 4441 4915 Size: 552 939 256 438 273 69 1401 474 Cuts at: 4915 513 Size: 3300 Fragments arranged by size: 3300 1401 939 552 474 438 273 256 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6963 Fragments arranged by size: 6963 683 56 BtsI GCAGTG_nn' Cuts at: 2306 2492 3638 3737 4219 5218 6012 6032 2306 Size: 186 1146 99 482 999 794 20 3976 Fragments arranged by size: 3976 1146 999 794 482 186 99 20 ClaI AT'CG_AT Cuts at: 1554 3909 1554 Size: 2355 5347 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7667 DraI TTT'AAA Cuts at: 5006 5006 Size: 7702 DraIII CAC_nnn'GTG Cuts at: 2487 4692 2487 Size: 2205 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2945 6709 809 Size: 507 1629 3764 1802 Fragments arranged by size: 3764 1802 1629 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2105 2254 2730 Size: 54 1425 50 275 18 275 149 476 Cuts at: 2730 3351 3747 4464 4912 5149 6038 7667 8 Size: 621 396 717 448 237 889 1629 43 Fragments arranged by size: 1629 1425 889 717 621 476 448 396 275 275 237 149 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2105 5149 1537 Size: 275 293 3044 4090 Fragments arranged by size: 4090 3044 293 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3410 5632 1353 Size: 2057 2222 3423 Fragments arranged by size: 3423 2222 2057 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2882 3011 3722 3835 4883 6289 Size: 1064 138 1293 129 711 113 1048 1406 Cuts at: 6289 6663 6809 387 Size: 374 146 1280 Fragments arranged by size: 1406 1293 1280 1064 1048 711 374 146 138 129 113 EcoICRI GAG'CTC Cuts at: 2512 2512 Size: 7702 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3280 3788 3885 4430 4478 5765 6362 Size: 393 1648 508 97 545 48 1287 597 Cuts at: 6362 7149 7417 1239 Size: 787 268 1524 Fragments arranged by size: 1648 1524 1287 787 597 545 508 393 268 97 48 EcoNI CCTnn'n_nnAGG Cuts at: 3563 3893 3563 Size: 330 7372 EcoO109I rG'GnC_Cy Cuts at: 2115 2578 2633 3781 3886 4309 4316 4317 4489 Size: 463 55 1148 105 423 7 1 172 Cuts at: 4489 4550 4569 4613 2115 Size: 61 19 44 5204 Fragments arranged by size: 5204 1148 463 423 172 105 61 55 44 19 7 1 EcoRI G'AATT_C Cuts at: 5011 5389 5011 Size: 378 7324 FspI TGC'GCA Cuts at: 4127 6208 4127 Size: 2081 5621 HaeII r_GCGC'y Cuts at: 1678 3101 3461 3502 4049 4262 4409 4419 4513 Size: 1423 360 41 547 213 147 10 94 Cuts at: 4513 4605 4655 4788 4953 6849 1678 Size: 92 50 133 165 1896 2531 Fragments arranged by size: 2531 1896 1423 547 360 213 165 147 133 94 92 50 41 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3809 3841 3982 4014 6343 Size: 32 135 32 2237 32 141 32 2329 Cuts at: 6343 6375 6417 6449 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2329 2237 141 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3808 4219 5046 5292 669 Size: 988 281 1870 411 827 246 3079 Fragments arranged by size: 3079 1870 988 827 411 281 246 HindIII A'AGCT_T Cuts at: 748 3401 748 Size: 2653 5049 HpaI GTT'AAC Cuts at: 5292 5292 Size: 7702 KasI G'GCGC_C Cuts at: 3498 4415 4601 3498 Size: 917 186 6599 Fragments arranged by size: 6599 917 186 KpnI G_GTAC'C Cuts at: 2757 5027 2757 Size: 2270 5432 MfeI C'AATT_G Cuts at: 5301 5301 Size: 7702 MluI A'CGCG_T Cuts at: 7578 7578 Size: 7702 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1991 4427 6816 7000 1305 Size: 686 2436 2389 184 2007 Fragments arranged by size: 2436 2389 2007 686 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2732 10 Size: 54 1425 343 900 4980 Fragments arranged by size: 4980 1425 900 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2243 2762 3680 3896 5660 6019 Size: 648 1011 66 519 918 216 1764 359 Cuts at: 6019 6178 518 Size: 159 2042 Fragments arranged by size: 2042 1764 1011 918 648 519 359 216 159 66 MspA1I CmG'CkG Cuts at: 1606 2819 3042 3286 4012 4110 4150 4276 4327 Size: 1213 223 244 726 98 40 126 51 Cuts at: 4327 4443 4456 4625 4816 5118 5795 6943 7188 Size: 116 13 169 191 302 677 1148 245 Cuts at: 7188 1606 Size: 2120 Fragments arranged by size: 2120 1213 1148 726 677 302 245 244 223 191 169 126 116 98 51 40 13 NaeI GCC'GGC Cuts at: 2335 2865 3871 2335 Size: 530 1006 6166 Fragments arranged by size: 6166 1006 530 NarI GG'CG_CC Cuts at: 3499 4416 4602 3499 Size: 917 186 6599 Fragments arranged by size: 6599 917 186 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6211 Fragments arranged by size: 6211 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7702 NgoMIV G'CCGG_C Cuts at: 2333 2863 3869 2333 Size: 530 1006 6166 Fragments arranged by size: 6166 1006 530 NheI G'CTAG_C Cuts at: 1052 3976 1052 Size: 2924 4778 NotI GC'GGCC_GC Cuts at: 5149 5149 Size: 7702 NspI r_CATG'y Cuts at: 1132 1229 2176 2497 4926 5060 6605 1132 Size: 97 947 321 2429 134 1545 2229 Fragments arranged by size: 2429 2229 1545 947 321 134 97 PasI CC'CwG_GG Cuts at: 1348 3217 3655 4313 1348 Size: 1869 438 658 4737 Fragments arranged by size: 4737 1869 658 438 PciI A'CATG_T Cuts at: 6601 6601 Size: 7702 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 4819 1108 Size: 902 2809 3991 Fragments arranged by size: 3991 2809 902 PfoI T'CCnGG_A Cuts at: 3765 4098 4515 3765 Size: 333 417 6952 Fragments arranged by size: 6952 417 333 PmeI GTTT'AAAC Cuts at: 5006 5006 Size: 7702 PmlI CAC'GTG Cuts at: 3745 4177 4255 4597 3745 Size: 432 78 342 6850 Fragments arranged by size: 6850 432 342 78 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3362 3394 5771 5803 7284 7316 3362 Size: 32 2377 32 1481 32 3748 Fragments arranged by size: 3748 2377 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2115 3886 4309 4569 2115 Size: 1771 423 260 5248 Fragments arranged by size: 5248 1771 423 260 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7702 PsiI TTA'TAA Cuts at: 5272 5272 Size: 7702 PspOMI G'GGCC_C Cuts at: 3408 3780 4316 4549 4613 3408 Size: 372 536 233 64 6497 Fragments arranged by size: 6497 536 372 233 64 PstI C_TGCA'G Cuts at: 830 2067 2877 3177 4143 4266 4833 5054 830 Size: 1237 810 300 966 123 567 221 3478 Fragments arranged by size: 3478 1237 966 810 567 300 221 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7702 PvuII CAG'CTG Cuts at: 4012 4276 4327 4816 4012 Size: 264 51 489 6898 Fragments arranged by size: 6898 489 264 51 RsrII CG'GwC_CG Cuts at: 3037 3037 Size: 7702 SacI G_AGCT'C Cuts at: 2514 2514 Size: 7702 SacII CC_GC'GG Cuts at: 3043 4444 3043 Size: 1401 6301 SalI G'TCGA_C Cuts at: 1936 5044 1936 Size: 3108 4594 SanDI GG'GwC_CC Cuts at: 4309 4569 4309 Size: 260 7442 SbfI CC_TGCA'GG Cuts at: 5054 5054 Size: 7702 ScaI AGT'ACT Cuts at: 5950 7662 5950 Size: 1712 5990 SfcI C'TryA_G Cuts at: 826 1045 2063 2873 3173 4139 4262 4340 4829 Size: 219 1018 810 300 966 123 78 489 Cuts at: 4829 5050 6185 6866 7057 826 Size: 221 1135 681 191 1471 Fragments arranged by size: 1471 1135 1018 966 810 681 489 300 221 219 191 123 78 SfoI GGC'GCC Cuts at: 3500 4417 4603 3500 Size: 917 186 6599 Fragments arranged by size: 6599 917 186 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7702 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7702 SmaI CCC'GGG Cuts at: 1872 3094 3365 3435 3476 4548 5029 1872 Size: 1222 271 70 41 1072 481 4545 Fragments arranged by size: 4545 1222 1072 481 271 70 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 2798 3962 4484 5806 6707 Size: 197 926 135 720 1164 522 1322 901 Cuts at: 6707 6969 820 Size: 262 1553 Fragments arranged by size: 1553 1322 1164 926 901 720 522 262 197 135 SnaBI TAC'GTA Cuts at: 493 5493 493 Size: 5000 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7702 SphI G_CATG'C Cuts at: 1132 2176 4926 5060 1132 Size: 1044 2750 134 3774 Fragments arranged by size: 3774 2750 1044 134 SrfI GCCC'GGGC Cuts at: 3476 3476 Size: 7702 SspI AAT'ATT Cuts at: 5 52 5626 5 Size: 47 5574 2081 Fragments arranged by size: 5574 2081 47 StuI AGG'CCT Cuts at: 1517 4346 1517 Size: 2829 4873 StyI C'CwwG_G Cuts at: 513 1065 2004 2849 3526 5141 513 Size: 552 939 845 677 1615 3074 Fragments arranged by size: 3074 1615 939 845 677 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3112 3687 3740 4197 5729 5746 5899 Size: 1255 1532 575 53 457 1532 17 153 Cuts at: 5899 6084 6529 325 Size: 185 445 1498 Fragments arranged by size: 1532 1532 1498 1255 575 457 445 185 153 53 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5948 7660 96 Size: 274 80 33 51 1443 3971 1712 138 Fragments arranged by size: 3971 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2186 2218 2440 2472 2551 2583 2607 2639 2786 Size: 32 222 32 79 32 24 32 147 Cuts at: 2786 2818 3250 3282 3429 3461 3941 3973 4193 Size: 32 432 32 147 32 480 32 220 Cuts at: 4193 4225 7662 7694 2186 Size: 32 3437 32 2194 Fragments arranged by size: 3437 2194 480 432 222 220 147 147 79 32 32 32 32 32 32 32 32 32 32 24 Tth111I GACn'n_nGTC Cuts at: 1316 2921 3134 1316 Size: 1605 213 5884 Fragments arranged by size: 5884 1605 213 XbaI T'CTAG_A Cuts at: 5038 5038 Size: 7702 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3346 3604 3346 Size: 258 7444 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7702 XmaI C'CCGG_G Cuts at: 1870 3092 3363 3433 3474 4546 5027 1870 Size: 1222 271 70 41 1072 481 4545 Fragments arranged by size: 4545 1222 1072 481 271 70 41 XmnI GAAnn'nnTTC Cuts at: 5831 5831 Size: 7702 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3805 5510 276 Size: 53 83 186 936 2271 1705 2468 Fragments arranged by size: 2468 2271 1705 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI SapI SexAI SfiI SwaI