(Linear) (Six-base) MAPSORT of: KIEE3580.seq Check: 8338 from: 1 to: 2997 KIEE3580.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 150 1494 2869 2997 Size: 150 1344 1375 128 Fragments arranged by size: 1375 1344 150 128 AatII G_ACGT'C Cuts at: 0 1802 2997 Size: 1802 1195 Acc65I G'GTAC_C Cuts at: 0 748 2997 Size: 748 2249 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1275 2473 2997 Size: 1275 1198 524 Fragments arranged by size: 1275 1198 524 AfeI AGC'GCT Cuts at: 0 2506 2997 Size: 2506 491 AleI CACnn'nnGTG Cuts at: 0 238 757 1675 1891 2997 Size: 238 519 918 216 1106 Fragments arranged by size: 1106 918 519 238 216 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1562 1750 2069 2767 2997 Size: 1562 188 319 698 230 Fragments arranged by size: 1562 698 319 230 188 AlwNI CAG_nnn'CTG Cuts at: 0 1273 2274 2325 2948 2997 Size: 1273 1001 51 623 49 Fragments arranged by size: 1273 1001 623 51 49 ApaI G_GGCC'C Cuts at: 0 1407 1779 2315 2548 2612 2997 Size: 1407 372 536 233 64 385 Fragments arranged by size: 1407 536 385 372 233 64 AvaI C'yCGr_G Cuts at: 0 113 579 1087 1321 1358 1428 1469 1663 Size: 113 466 508 234 37 70 41 194 Cuts at: 1663 1994 2343 2541 2560 2611 2997 Size: 331 349 198 19 51 386 Fragments arranged by size: 508 466 386 349 331 234 198 194 113 70 51 41 37 19 BamHI G'GATC_C Cuts at: 0 584 2997 Size: 584 2413 BanI G'GyrC_C Cuts at: 0 386 748 1012 1493 1694 1893 2181 2410 Size: 386 362 264 481 201 199 288 229 Cuts at: 2410 2596 2726 2997 Size: 186 130 271 Fragments arranged by size: 481 386 362 288 271 264 229 201 199 186 130 BanII G_rGCy'C Cuts at: 0 509 649 1407 1476 1779 2315 2449 2548 Size: 509 140 758 69 303 536 134 99 Cuts at: 2548 2612 2997 Size: 64 385 Fragments arranged by size: 758 536 509 385 303 140 134 99 69 64 BbeI G_GCGC'C Cuts at: 0 1497 2414 2600 2997 Size: 1497 917 186 397 Fragments arranged by size: 1497 917 397 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 270 304 603 637 1312 1346 2997 Size: 270 34 299 34 675 34 1651 Fragments arranged by size: 1651 675 299 270 34 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 88 257 971 1849 2354 2762 2997 Size: 88 169 714 878 505 408 235 Fragments arranged by size: 878 714 505 408 235 169 88 BglII A'GATC_T Cuts at: 0 2987 2997 Size: 2987 10 BlpI GC'TnA_GC Cuts at: 0 2941 2997 Size: 2941 56 Bme1580I G_kGCm'C Cuts at: 0 235 310 1015 1407 1779 1858 2315 2548 Size: 235 75 705 392 372 79 457 233 Cuts at: 2548 2612 2997 Size: 64 385 Fragments arranged by size: 705 457 392 385 372 235 233 79 75 64 BmgBI CAC'GTC Cuts at: 0 737 1044 1128 1344 2112 2418 2997 Size: 737 307 84 216 768 306 579 Fragments arranged by size: 768 737 579 307 306 216 84 BmrI ACTGGGnnnn_n' Cuts at: 0 645 2997 Size: 645 2352 BmtI G_CTAG'C Cuts at: 0 1975 2997 Size: 1975 1022 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 663 2997 Size: 663 2334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1783 1880 2425 2997 Size: 1783 97 545 572 Fragments arranged by size: 1783 572 545 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 778 1942 2464 2997 Size: 58 720 1164 522 533 Fragments arranged by size: 1164 720 533 522 58 BsaI GGTCTCn'nnnn_ Cuts at: 0 526 2997 Size: 526 2471 BsaAI yAC'GTr Cuts at: 0 1740 2172 2250 2592 2997 Size: 1740 432 78 342 405 Fragments arranged by size: 1740 432 405 342 78 BsaBI GATnn'nnATC Cuts at: 0 2089 2997 Size: 2089 908 BsaHI Gr'CG_yC Cuts at: 0 380 1494 1706 1799 1814 2015 2411 2597 Size: 380 1114 212 93 15 201 396 186 Cuts at: 2597 2997 Size: 400 Fragments arranged by size: 1114 400 396 380 212 201 186 93 15 BsaWI w'CCGG_w Cuts at: 0 2126 2997 Size: 2126 871 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 602 632 1936 1966 2997 Size: 602 30 1304 30 1031 Fragments arranged by size: 1304 1031 602 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1339 2242 2707 2833 2997 Size: 1339 903 465 126 164 Fragments arranged by size: 1339 903 465 164 126 BseYI C'CCAG_C Cuts at: 0 13 698 1174 1455 1690 2200 2501 2997 Size: 13 685 476 281 235 510 301 496 Fragments arranged by size: 685 510 496 476 301 281 235 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 313 459 1072 1621 1720 1768 1819 Size: 178 135 146 613 549 99 48 51 Cuts at: 1819 1927 2226 2230 2572 2997 Size: 108 299 4 342 425 Fragments arranged by size: 613 549 425 342 299 178 146 135 108 99 51 48 4 BsiEI CG_ry'CG Cuts at: 0 103 279 525 2387 2442 2979 2997 Size: 103 176 246 1862 55 537 18 Fragments arranged by size: 1862 537 246 176 103 55 18 BsiHKAI G_wGCw'C Cuts at: 0 509 1427 1682 1729 2480 2638 2997 Size: 509 918 255 47 751 158 359 Fragments arranged by size: 918 751 509 359 255 158 47 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1060 1241 2997 Size: 1060 181 1756 Fragments arranged by size: 1756 1060 181 Bsp1286I G_dGCh'C Cuts at: 0 235 310 509 649 1015 1407 1427 1476 Size: 235 75 199 140 366 392 20 49 Cuts at: 1476 1682 1729 1779 1858 2315 2449 2480 2548 Size: 206 47 50 79 457 134 31 68 Cuts at: 2548 2612 2638 2997 Size: 64 26 359 Fragments arranged by size: 457 392 366 359 235 206 199 140 134 79 75 68 64 50 49 47 31 26 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 150 359 1265 1494 1616 1814 2594 2869 Size: 150 209 906 229 122 198 780 275 Cuts at: 2869 2997 Size: 128 Fragments arranged by size: 906 780 275 229 209 198 150 128 122 BsrBI CCG'CTC Cuts at: 0 538 1534 2517 2997 Size: 538 996 983 480 Fragments arranged by size: 996 983 538 480 BsrFI r'CCGG_y Cuts at: 0 328 858 1316 1864 2837 2997 Size: 328 530 458 548 973 160 Fragments arranged by size: 973 548 530 458 328 160 BssHII G'CGCG_C Cuts at: 0 341 1353 2699 2701 2997 Size: 341 1012 1346 2 296 Fragments arranged by size: 1346 1012 341 296 2 BssSI C'ACGA_G Cuts at: 0 1063 2893 2997 Size: 1063 1830 104 Fragments arranged by size: 1830 1063 104 BstAPI GCAn_nnn'nTGC Cuts at: 0 351 426 482 2726 2861 2997 Size: 351 75 56 2244 135 136 Fragments arranged by size: 2244 351 136 135 75 56 BstYI r'GATC_y Cuts at: 0 584 2084 2987 2997 Size: 584 1500 903 10 Fragments arranged by size: 1500 903 584 10 BtgI C'CryG_G Cuts at: 0 255 693 966 1035 2436 2910 2997 Size: 255 438 273 69 1401 474 87 Fragments arranged by size: 1401 474 438 273 255 87 69 BtsI GCAGTG_nn' Cuts at: 0 301 487 1633 1732 2214 2997 Size: 301 186 1146 99 482 783 Fragments arranged by size: 1146 783 482 301 186 99 ClaI AT'CG_AT Cuts at: 0 1904 2997 Size: 1904 1093 DraIII CAC_nnn'GTG Cuts at: 0 482 2687 2997 Size: 482 2205 310 Fragments arranged by size: 2205 482 310 DrdI GACnn_nn'nnGTC Cuts at: 0 940 2997 Size: 940 2057 EaeI y'GGCC_r Cuts at: 0 100 249 725 1346 1742 2459 2907 2997 Size: 100 149 476 621 396 717 448 90 Fragments arranged by size: 717 621 476 448 396 149 100 90 EagI C'GGCC_G Cuts at: 0 100 2997 Size: 100 2897 EarI CTCTTCn'nnn_ Cuts at: 0 1405 2997 Size: 1405 1592 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 877 1006 1717 1830 2878 2997 Size: 877 129 711 113 1048 119 Fragments arranged by size: 1048 877 711 129 119 113 EcoICRI GAG'CTC Cuts at: 0 507 2997 Size: 507 2490 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1275 1783 1880 2425 2473 2997 Size: 1275 508 97 545 48 524 Fragments arranged by size: 1275 545 524 508 97 48 EcoNI CCTnn'n_nnAGG Cuts at: 0 1558 1888 2997 Size: 1558 330 1109 Fragments arranged by size: 1558 1109 330 EcoO109I rG'GnC_Cy Cuts at: 0 110 573 628 1776 1881 2304 2311 2312 Size: 110 463 55 1148 105 423 7 1 Cuts at: 2312 2484 2545 2564 2608 2997 Size: 172 61 19 44 389 Fragments arranged by size: 1148 463 423 389 172 110 105 61 55 44 19 7 1 FspI TGC'GCA Cuts at: 0 2122 2997 Size: 2122 875 HaeII r_GCGC'y Cuts at: 0 1096 1456 1497 2044 2257 2404 2414 2508 Size: 1096 360 41 547 213 147 10 94 Cuts at: 2508 2600 2650 2783 2948 2997 Size: 92 50 133 165 49 Fragments arranged by size: 1096 547 360 213 165 147 133 94 92 50 49 41 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1804 1836 1977 2009 2997 Size: 1804 32 141 32 988 Fragments arranged by size: 1804 988 141 32 32 HincII GTy'rAC Cuts at: 0 1803 2214 2997 Size: 1803 411 783 Fragments arranged by size: 1803 783 411 HindIII A'AGCT_T Cuts at: 0 1396 2997 Size: 1396 1601 KasI G'GCGC_C Cuts at: 0 1493 2410 2596 2997 Size: 1493 917 186 401 Fragments arranged by size: 1493 917 401 186 KpnI G_GTAC'C Cuts at: 0 752 2997 Size: 752 2245 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2422 2997 Size: 2422 575 MscI TGG'CCA Cuts at: 0 727 2997 Size: 727 2270 MslI CAynn'nnrTG Cuts at: 0 172 238 757 1675 1891 2997 Size: 172 66 519 918 216 1106 Fragments arranged by size: 1106 918 519 216 172 66 MspA1I CmG'CkG Cuts at: 0 814 1037 1281 2007 2105 2145 2271 2322 Size: 814 223 244 726 98 40 126 51 Cuts at: 2322 2438 2451 2620 2811 2997 Size: 116 13 169 191 186 Fragments arranged by size: 814 726 244 223 191 186 169 126 116 98 51 40 13 NaeI GCC'GGC Cuts at: 0 330 860 1866 2997 Size: 330 530 1006 1131 Fragments arranged by size: 1131 1006 530 330 NarI GG'CG_CC Cuts at: 0 1494 2411 2597 2997 Size: 1494 917 186 400 Fragments arranged by size: 1494 917 400 186 NgoMIV G'CCGG_C Cuts at: 0 328 858 1864 2997 Size: 328 530 1006 1133 Fragments arranged by size: 1133 1006 530 328 NheI G'CTAG_C Cuts at: 0 1971 2997 Size: 1971 1026 NspI r_CATG'y Cuts at: 0 171 492 2921 2997 Size: 171 321 2429 76 Fragments arranged by size: 2429 321 171 76 PasI CC'CwG_GG Cuts at: 0 1212 1650 2308 2997 Size: 1212 438 658 689 Fragments arranged by size: 1212 689 658 438 PflMI CCAn_nnn'nTGG Cuts at: 0 2814 2997 Size: 2814 183 PfoI T'CCnGG_A Cuts at: 0 1760 2093 2510 2997 Size: 1760 333 417 487 Fragments arranged by size: 1760 487 417 333 PmlI CAC'GTG Cuts at: 0 1740 2172 2250 2592 2997 Size: 1740 432 78 342 405 Fragments arranged by size: 1740 432 405 342 78 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1357 1389 2997 Size: 1357 32 1608 Fragments arranged by size: 1608 1357 32 PpuMI rG'GwC_Cy Cuts at: 0 110 1881 2304 2564 2997 Size: 110 1771 423 260 433 Fragments arranged by size: 1771 433 423 260 110 PspOMI G'GGCC_C Cuts at: 0 1403 1775 2311 2544 2608 2997 Size: 1403 372 536 233 64 389 Fragments arranged by size: 1403 536 389 372 233 64 PstI C_TGCA'G Cuts at: 0 62 872 1172 2138 2261 2828 2997 Size: 62 810 300 966 123 567 169 Fragments arranged by size: 966 810 567 300 169 123 62 PvuII CAG'CTG Cuts at: 0 2007 2271 2322 2811 2997 Size: 2007 264 51 489 186 Fragments arranged by size: 2007 489 264 186 51 RsrII CG'GwC_CG Cuts at: 0 1032 2997 Size: 1032 1965 SacI G_AGCT'C Cuts at: 0 509 2997 Size: 509 2488 SacII CC_GC'GG Cuts at: 0 1038 2439 2997 Size: 1038 1401 558 Fragments arranged by size: 1401 1038 558 SanDI GG'GwC_CC Cuts at: 0 2304 2564 2997 Size: 2304 260 433 Fragments arranged by size: 2304 433 260 SfcI C'TryA_G Cuts at: 0 58 868 1168 2134 2257 2335 2824 2997 Size: 58 810 300 966 123 78 489 173 Fragments arranged by size: 966 810 489 300 173 123 78 58 SfoI GGC'GCC Cuts at: 0 1495 2412 2598 2997 Size: 1495 917 186 399 Fragments arranged by size: 1495 917 399 186 SmaI CCC'GGG Cuts at: 0 1089 1360 1430 1471 2543 2997 Size: 1089 271 70 41 1072 454 Fragments arranged by size: 1089 1072 454 271 70 41 SmlI C'TyrA_G Cuts at: 0 73 793 1957 2479 2997 Size: 73 720 1164 522 518 Fragments arranged by size: 1164 720 522 518 73 SphI G_CATG'C Cuts at: 0 171 2921 2997 Size: 171 2750 76 Fragments arranged by size: 2750 171 76 SrfI GCCC'GGGC Cuts at: 0 1471 2997 Size: 1471 1526 StuI AGG'CCT Cuts at: 0 2341 2997 Size: 2341 656 StyI C'CwwG_G Cuts at: 0 844 1521 2997 Size: 844 677 1476 Fragments arranged by size: 1476 844 677 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1107 1682 1735 2192 2997 Size: 1107 575 53 457 805 Fragments arranged by size: 1107 805 575 457 53 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 181 213 435 467 546 578 602 634 Size: 181 32 222 32 79 32 24 32 Cuts at: 634 781 813 1245 1277 1424 1456 1936 1968 Size: 147 32 432 32 147 32 480 32 Cuts at: 1968 2188 2220 2997 Size: 220 32 777 Fragments arranged by size: 777 480 432 222 220 181 147 147 79 32 32 32 32 32 32 32 32 32 24 Tth111I GACn'n_nGTC Cuts at: 0 916 1129 2997 Size: 916 213 1868 Fragments arranged by size: 1868 916 213 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1341 1599 2997 Size: 1341 258 1398 Fragments arranged by size: 1398 1341 258 XmaI C'CCGG_G Cuts at: 0 1087 1358 1428 1469 2541 2997 Size: 1087 271 70 41 1072 456 Fragments arranged by size: 1087 1072 456 271 70 41 ZraI GAC'GTC Cuts at: 0 1800 2997 Size: 1800 1197 Enzymes that do cut: AarI AatII Acc65I AcuI AfeI AleI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstYI BtgI BtsI ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII RsrII SacI SacII SanDI SfcI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AccI AclI AflII AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI BclI BfrBI Bpu10I BsiWI BsmI BspEI BspHI BsrDI BsrGI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI CspCI DraI EcoRI EcoRV FalI FseI FspAI HpaI MfeI MluI NcoI NdeI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI TatI XbaI XhoI XmnI