(Circular) (Six-base) MAPSORT of: pFN21AB6386.seq Check: 6472 from: 1 to: 5608 pFN21AB6386 5608 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2453 2453 Size: 5608 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3418 278 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2663 2951 1937 Size: 726 288 4594 Fragments arranged by size: 4594 726 288 Acc65I G'GTAC_C Cuts at: 2929 2929 Size: 5608 AclI AA'CG_TT Cuts at: 1563 3735 4108 1563 Size: 2172 373 3063 Fragments arranged by size: 3063 2172 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3671 5055 5323 1632 Size: 2039 1384 268 1917 Fragments arranged by size: 2039 1917 1384 268 AfeI AGC'GCT Cuts at: 2135 2135 Size: 5608 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5411 AflIII A'CryG_T Cuts at: 1203 4507 5484 1203 Size: 3304 977 1327 Fragments arranged by size: 3304 1327 977 AgeI A'CCGG_T Cuts at: 4439 4439 Size: 5608 AhdI GACnn_n'nnGTC Cuts at: 4337 4337 Size: 5608 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5608 AlwNI CAG_nnn'CTG Cuts at: 2459 3351 4923 2459 Size: 892 1572 3144 Fragments arranged by size: 3144 1572 892 ApaI G_GGCC'C Cuts at: 2446 2446 Size: 5608 ApaLI G'TGCA_C Cuts at: 2701 3665 4821 2701 Size: 964 1156 3488 Fragments arranged by size: 3488 1156 964 ApoI r'AATT_y Cuts at: 1493 2830 2917 3143 3295 5404 1493 Size: 1337 87 226 152 2109 1697 Fragments arranged by size: 2109 1697 1337 226 152 87 AseI AT'TA_AT Cuts at: 160 1032 4162 160 Size: 872 3130 1606 Fragments arranged by size: 3130 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 AvaI C'yCGr_G Cuts at: 1870 1943 2933 1870 Size: 73 990 4545 Fragments arranged by size: 4545 990 73 BamHI G'GATC_C Cuts at: 2938 3305 2938 Size: 367 5241 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2783 2929 4384 618 Size: 325 217 627 996 146 1455 1842 Fragments arranged by size: 1842 1455 996 627 325 217 146 BanII G_rGCy'C Cuts at: 1391 2302 2446 2927 1391 Size: 911 144 481 4072 Fragments arranged by size: 4072 911 481 144 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2766 928 Size: 971 867 3770 Fragments arranged by size: 3770 971 867 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2692 2726 3799 3833 1903 Size: 34 755 34 1073 34 3678 Fragments arranged by size: 3678 1073 755 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3500 4710 3500 Size: 1210 4398 BclI T'GATC_A Cuts at: 1548 1548 Size: 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4219 136 Size: 107 122 71 631 605 2547 1525 Fragments arranged by size: 2547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5603 5603 Size: 5608 BlpI GC'TnA_GC Cuts at: 3025 3025 Size: 5608 Bme1580I G_kGCm'C Cuts at: 1790 2446 2705 3669 4825 1790 Size: 656 259 964 1156 2573 Fragments arranged by size: 2573 1156 964 656 259 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5608 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2082 3339 4297 Size: 416 53 83 399 24 665 1257 958 Cuts at: 4297 442 Size: 1753 Fragments arranged by size: 1753 1257 958 665 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2624 1056 Size: 1568 4040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2702 2765 2855 4268 1239 Size: 1178 285 63 90 1413 2579 Fragments arranged by size: 2579 1413 1178 285 90 63 Bpu10I CC'TnA_GC Cuts at: 2301 2301 Size: 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3733 4598 4896 3733 Size: 865 298 4445 Fragments arranged by size: 4445 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2419 4271 882 Size: 617 920 1852 2219 Fragments arranged by size: 2219 1852 920 617 BsaAI yAC'GTr Cuts at: 493 1204 2047 2638 2816 3399 493 Size: 711 843 591 178 583 2702 Fragments arranged by size: 2702 843 711 591 583 178 BsaBI GATnn'nnATC Cuts at: 5602 5602 Size: 5608 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3415 3797 4432 275 Size: 53 83 186 936 1882 382 635 1451 Fragments arranged by size: 1882 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2433 4041 4439 4713 4860 5271 2433 Size: 1608 398 274 147 411 2770 Fragments arranged by size: 2770 1608 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5383 Fragments arranged by size: 5383 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2810 1186 Size: 1033 591 3984 Fragments arranged by size: 3984 1033 591 BseYI C'CCAG_C Cuts at: 2836 4811 5307 2836 Size: 1975 496 3137 Fragments arranged by size: 3137 1975 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5035 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3058 3819 4847 1540 Size: 275 186 1057 761 1028 2301 Fragments arranged by size: 2301 1057 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2155 2302 2705 2721 3669 3754 4825 2155 Size: 147 403 16 948 85 1071 2938 Fragments arranged by size: 2938 1071 948 403 147 85 16 BsmI GAATG_Cn' Cuts at: 3119 3212 3119 Size: 93 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2302 2446 2705 2721 2927 3669 Size: 399 365 147 144 259 16 206 742 Cuts at: 3669 3754 4825 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 365 259 206 147 144 85 16 BspEI T'CCGG_A Cuts at: 5271 5271 Size: 5608 BspHI T'CATG_A Cuts at: 3497 5227 3497 Size: 1730 3878 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2453 2963 844 Size: 1609 510 3489 Fragments arranged by size: 3489 1609 510 BsrBI CCG'CTC Cuts at: 1394 1823 3495 1394 Size: 429 1672 3507 Fragments arranged by size: 3507 1672 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2503 4103 4277 65 Size: 1183 1255 1600 174 1396 Fragments arranged by size: 1600 1396 1255 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2142 4252 4439 1700 Size: 110 332 2110 187 2869 Fragments arranged by size: 2869 2110 332 187 110 BsrGI T'GTAC_A Cuts at: 96 2425 96 Size: 2329 3279 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5608 BssSI C'ACGA_G Cuts at: 3668 4680 3668 Size: 1012 4596 BstAPI GCAn_nnn'nTGC Cuts at: 794 2459 2630 794 Size: 1665 171 3772 Fragments arranged by size: 3772 1665 171 BstBI TT'CG_AA Cuts at: 2712 4412 2712 Size: 1700 3908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5608 BstXI CCAn_nnnn'nTGG Cuts at: 2167 2167 Size: 5608 BstYI r'GATC_y Cuts at: 1972 2400 2860 2938 3305 3690 3707 5159 5245 Size: 428 460 78 367 385 17 1452 86 Cuts at: 5245 5257 5603 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 460 428 385 367 346 86 78 17 12 BstZ17I GTA'TAC Cuts at: 2664 2664 Size: 5608 BtgI C'CryG_G Cuts at: 513 1065 2004 2166 513 Size: 552 939 162 3955 Fragments arranged by size: 3955 939 552 162 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2464 487 Size: 683 56 1238 3631 Fragments arranged by size: 3631 1238 683 56 BtsI GCAGTG_nn' Cuts at: 2883 3124 3918 3938 2883 Size: 241 794 20 4553 Fragments arranged by size: 4553 794 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5608 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5573 DraI TTT'AAA Cuts at: 2912 2912 Size: 5608 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4615 809 Size: 507 3299 1802 Fragments arranged by size: 3299 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 3055 3944 Size: 54 1425 50 275 18 273 952 889 Cuts at: 3944 5573 8 Size: 1629 43 Fragments arranged by size: 1629 1425 952 889 275 273 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3055 1537 Size: 275 1243 4090 Fragments arranged by size: 4090 1243 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2564 2709 3538 1353 Size: 1211 145 829 3423 Fragments arranged by size: 3423 1211 829 145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2186 4195 4569 4715 387 Size: 1064 138 597 2009 374 146 1280 Fragments arranged by size: 2009 1280 1064 597 374 146 138 EcoICRI GAG'CTC Cuts at: 2300 2300 Size: 5608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2702 2765 2855 3671 4268 5055 Size: 393 785 285 63 90 816 597 787 Cuts at: 5055 5323 1239 Size: 268 1524 Fragments arranged by size: 1524 816 787 785 597 393 285 268 90 63 EcoO109I rG'GnC_Cy Cuts at: 2116 2443 2749 2116 Size: 327 306 4975 Fragments arranged by size: 4975 327 306 EcoRI G'AATT_C Cuts at: 2917 3295 2917 Size: 378 5230 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2569 2569 Size: 5608 FspI TGC'GCA Cuts at: 4114 4114 Size: 5608 HaeII r_GCGC'y Cuts at: 1678 2137 4755 1678 Size: 459 2618 2531 Fragments arranged by size: 2618 2531 459 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2689 2721 4249 4281 4323 Size: 32 135 32 1117 32 1528 32 42 Cuts at: 4323 4355 1373 Size: 32 2626 Fragments arranged by size: 2626 1528 1117 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2952 3198 669 Size: 988 281 1014 246 3079 Fragments arranged by size: 3079 1014 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5608 HpaI GTT'AAC Cuts at: 3198 3198 Size: 5608 KpnI G_GTAC'C Cuts at: 2933 2933 Size: 5608 MfeI C'AATT_G Cuts at: 3207 3207 Size: 5608 MluI A'CGCG_T Cuts at: 5484 5484 Size: 5608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2184 4722 4906 1305 Size: 879 2538 184 2007 Fragments arranged by size: 2538 2007 879 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3786 Fragments arranged by size: 3786 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3566 3925 4084 518 Size: 648 2400 359 159 2042 Fragments arranged by size: 2400 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2368 2539 3024 3701 4849 5094 1606 Size: 762 171 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 762 677 485 245 171 NcoI C'CATG_G Cuts at: 513 1065 2004 2166 513 Size: 552 939 162 3955 Fragments arranged by size: 3955 939 552 162 NdeI CA'TA_TG Cuts at: 387 387 Size: 5608 NheI G'CTAG_C Cuts at: 1052 2620 1052 Size: 1568 4040 NotI GC'GGCC_GC Cuts at: 3055 3055 Size: 5608 NspI r_CATG'y Cuts at: 1132 1229 2966 4511 1132 Size: 97 1737 1545 2229 Fragments arranged by size: 2229 1737 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5608 PciI A'CATG_T Cuts at: 4507 4507 Size: 5608 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5608 PmeI GTTT'AAAC Cuts at: 2912 2912 Size: 5608 PmlI CAC'GTG Cuts at: 2047 2047 Size: 5608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3677 3709 5190 5222 3677 Size: 32 1481 32 4063 Fragments arranged by size: 4063 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2749 2749 Size: 5608 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5608 PsiI TTA'TAA Cuts at: 3178 3178 Size: 5608 PspOMI G'GGCC_C Cuts at: 2442 2442 Size: 5608 PstI C_TGCA'G Cuts at: 830 2464 2960 830 Size: 1634 496 3478 Fragments arranged by size: 3478 1634 496 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5608 PvuII CAG'CTG Cuts at: 2539 2539 Size: 5608 SacI G_AGCT'C Cuts at: 2302 2302 Size: 5608 SalI G'TCGA_C Cuts at: 1936 2950 1936 Size: 1014 4594 SapI GCTCTTCn'nnn_ Cuts at: 2709 2709 Size: 5608 SbfI CC_TGCA'GG Cuts at: 2464 2960 2464 Size: 496 5112 ScaI AGT'ACT Cuts at: 3856 5568 3856 Size: 1712 3896 SfcI C'TryA_G Cuts at: 826 1045 2460 2956 4091 4772 4963 826 Size: 219 1415 496 1135 681 191 1471 Fragments arranged by size: 1471 1415 1135 681 496 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5608 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5608 SmaI CCC'GGG Cuts at: 1872 2935 1872 Size: 1063 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3712 4613 4875 820 Size: 197 926 1769 901 262 1553 Fragments arranged by size: 1769 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2816 3399 493 Size: 2323 583 2702 Fragments arranged by size: 2702 2323 583 SpeI A'CTAG_T Cuts at: 152 152 Size: 5608 SphI G_CATG'C Cuts at: 1132 2966 1132 Size: 1834 3774 SspI AAT'ATT Cuts at: 5 52 3532 5 Size: 47 3480 2081 Fragments arranged by size: 3480 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5608 StyI C'CwwG_G Cuts at: 513 1065 2004 2166 3047 513 Size: 552 939 162 881 3074 Fragments arranged by size: 3074 939 881 552 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2736 3635 3652 3805 3990 4435 325 Size: 1255 1156 899 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1156 899 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2425 3854 5566 Size: 274 80 33 51 1443 448 1429 1712 Cuts at: 5566 96 Size: 138 Fragments arranged by size: 1712 1443 1429 448 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2355 2387 5568 5600 2355 Size: 32 3181 32 2363 Fragments arranged by size: 3181 2363 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5608 XbaI T'CTAG_A Cuts at: 2944 2944 Size: 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5608 XmaI C'CCGG_G Cuts at: 1870 2933 1870 Size: 1063 4545 XmnI GAAnn'nnTTC Cuts at: 2831 3737 2831 Size: 906 4702 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3416 276 Size: 53 83 186 936 1882 2468 Fragments arranged by size: 2468 1882 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI