Restriction map for
pFN21AB7615
(Circular) (Six-base) MAPSORT of: pFN21AB7615.seq Check: 8109 from: 1 to: 5617
pFN21AB7615 5617 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2479 2479
Size: 5617
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 1937
Size: 1023 4594
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3680 5064 5332 1632
Size: 2048 1384 268 1917
Fragments arranged by size:
2048 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 4516 5493 1203
Size: 3313 977 1327
Fragments arranged by size:
3313 1327 977
AgeI A'CCGG_T
Cuts at: 4448 4448
Size: 5617
AhdI GACnn_n'nnGTC
Cuts at: 2110 4346 2110
Size: 2236 3381
AleI CACnn'nnGTG
Cuts at: 1166 2886 1166
Size: 1720 3897
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2775 1184
Size: 1591 4026
AlwNI CAG_nnn'CTG
Cuts at: 2266 2328 2464 3360 4932 2266
Size: 62 136 896 1572 2951
Fragments arranged by size:
2951 1572 896 136 62
ApaI G_GGCC'C
Cuts at: 2355 2355
Size: 5617
ApaLI G'TGCA_C
Cuts at: 3674 4830 3674
Size: 1156 4461
ApoI r'AATT_y
Cuts at: 1493 2618 2926 3152 3304 5413 1493
Size: 1125 308 226 152 2109 1697
Fragments arranged by size:
2109 1697 1125 308 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2766 2942 1870
Size: 73 177 646 176 4545
Fragments arranged by size:
4545 646 177 176 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2205 2280 2938 4393 618
Size: 325 217 627 418 75 658 1455 1842
Fragments arranged by size:
1842 1455 658 627 418 325 217 75
BanII G_rGCy'C
Cuts at: 1391 2127 2355 2411 2936 1391
Size: 736 228 56 525 4072
Fragments arranged by size:
4072 736 525 228 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2289 2880 928
Size: 971 390 591 3665
Fragments arranged by size:
3665 971 591 390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2153 3509 4719 2153
Size: 1356 1210 3051
Fragments arranged by size:
3051 1356 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2099 5612 2099
Size: 3513 2104
BlpI GC'TnA_GC
Cuts at: 3034 3034
Size: 5617
Bme1580I G_kGCm'C
Cuts at: 1790 2208 2355 3678 4834 1790
Size: 418 147 1323 1156 2573
Fragments arranged by size:
2573 1323 1156 418 147
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5617
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2433 3348 4306
Size: 416 53 83 399 24 1016 915 958
Cuts at: 4306 442
Size: 1753
Fragments arranged by size:
1753 1016 958 915 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2331 2331
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2318 2367 2395 2828 4277 1239
Size: 1079 49 28 433 1449 2579
Fragments arranged by size:
2579 1449 1079 433 49 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 4607 4905 3742
Size: 865 298 4454
Fragments arranged by size:
4454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3424 3806 4441 275
Size: 53 83 186 936 1891 382 635 1451
Fragments arranged by size:
1891 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4050 4448 4722 4869 5280 4050
Size: 398 274 147 411 4387
Fragments arranged by size:
4387 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2054 2084 1178
Size: 30 165 30 651 30 4711
Fragments arranged by size:
4711 651 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5617
BseYI C'CCAG_C
Cuts at: 4820 5316 4820
Size: 496 5121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2853 1163
Size: 573 1117 3927
Fragments arranged by size:
3927 1117 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2455 3067 3828 4856 1540
Size: 275 186 454 612 761 1028 2301
Fragments arranged by size:
2301 1028 761 612 454 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2390 3678 3763 4834 2127
Size: 263 1288 85 1071 2910
Fragments arranged by size:
2910 1288 1071 263 85
BsmI GAATG_Cn'
Cuts at: 3128 3221 3128
Size: 93 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2208 2355 2390 2411 2936 3678
Size: 399 337 81 147 35 21 525 742
Cuts at: 3678 3763 4834 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 525 399 337 147 85
81 35 21
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2479 2972 844
Size: 1635 493 3489
Fragments arranged by size:
3489 1635 493
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 3504 1394
Size: 429 194 1487 3507
Fragments arranged by size:
3507 1487 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 4112 4286 65
Size: 1183 2864 174 1396
Fragments arranged by size:
2864 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4261 4448 1700
Size: 110 2451 187 2869
Fragments arranged by size:
2869 2451 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 2436 1925
Size: 511 5106
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 2621 794
Size: 1265 562 3790
Fragments arranged by size:
3790 1265 562
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2099 2677 2947 3314 3699 3716 5168 5254
Size: 127 578 270 367 385 17 1452 86
Cuts at: 5254 5266 5612 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 578 385 367 346 270 127
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 2092 513
Size: 552 939 42 46 4038
Fragments arranged by size:
4038 939 552 46 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 2673 3133 3927 3947 2673
Size: 460 794 20 4343
Fragments arranged by size:
4343 794 460 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DraIII CAC_nnn'GTG
Cuts at: 2309 2309
Size: 5617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4624 809
Size: 507 3308 1802
Fragments arranged by size:
3308 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 3064 3953
Size: 54 1425 50 275 18 622 612 889
Cuts at: 3953 5582 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 622 612 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2452 3064 1537
Size: 275 640 612 4090
Fragments arranged by size:
4090 640 612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2166 2401 2726 3547 1353
Size: 813 235 325 821 3423
Fragments arranged by size:
3423 821 813 325 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2495 2734 4204 4578 4724 387
Size: 1064 138 906 239 1470 374 146 1280
Fragments arranged by size:
1470 1280 1064 906 374 239 146 138
EcoICRI GAG'CTC
Cuts at: 2125 2125
Size: 5617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2318 2367 2395 2828 3680 4277 5064
Size: 393 686 49 28 433 852 597 787
Cuts at: 5064 5332 1239
Size: 268 1524
Fragments arranged by size:
1524 852 787 686 597 433 393 268
49 28
EcoO109I rG'GnC_Cy
Cuts at: 2351 2352 2351
Size: 1 5616
EcoRI G'AATT_C
Cuts at: 2926 3304 2926
Size: 378 5239
FspI TGC'GCA
Cuts at: 2033 2910 4123 2033
Size: 877 1213 3527
Fragments arranged by size:
3527 1213 877
FspAI rTGC'GCAy
Cuts at: 2910 2910
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 4764 1678
Size: 3086 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2882 2914 4258
Size: 32 135 32 524 32 754 32 1344
Cuts at: 4258 4290 4332 4364 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1344 754 524 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2554 2961 3207 669
Size: 988 281 616 407 246 3079
Fragments arranged by size:
3079 988 616 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2415 4731 4915 1305
Size: 1110 2316 184 2007
Fragments arranged by size:
2316 2007 1110 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2886 3575 3934 4093 518
Size: 648 1720 689 359 159 2042
Fragments arranged by size:
2042 1720 689 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3033 3710 4858 5103 1606
Size: 1427 677 1148 245 2120
Fragments arranged by size:
2120 1427 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2092 513
Size: 552 939 88 4038
Fragments arranged by size:
4038 939 552 88
NdeI CA'TA_TG
Cuts at: 387 2109 387
Size: 1722 3895
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2182 2909 2975 4520 1132
Size: 97 953 727 66 1545 2229
Fragments arranged by size:
2229 1545 953 727 97 66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5617
PciI A'CATG_T
Cuts at: 4516 4516
Size: 5617
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5617
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2001 2033 3686 3718 5199 5231 2001
Size: 32 1653 32 1481 32 2387
Fragments arranged by size:
2387 1653 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PspOMI G'GGCC_C
Cuts at: 2351 2351
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2969 830
Size: 2139 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
SacI G_AGCT'C
Cuts at: 2127 2127
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2862 2965 4100 4781 4972 826
Size: 219 1817 103 1135 681 191 1471
Fragments arranged by size:
1817 1471 1135 681 219 191 103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2944 1872
Size: 1072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3721 4622 4884 820
Size: 197 926 1778 901 262 1553
Fragments arranged by size:
1778 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2909 2975 1132
Size: 1777 66 3774
Fragments arranged by size:
3774 1777 66
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 2004 2092 3056 513
Size: 552 939 88 964 3074
Fragments arranged by size:
3074 964 939 552 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3644 3661 3814 3999 4444 325
Size: 1255 2064 17 153 185 445 1498
Fragments arranged by size:
2064 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2054 2086 5577 5609 2054
Size: 32 3491 32 2062
Fragments arranged by size:
3491 2062 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2342 2342
Size: 5617
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2942 1870
Size: 1072 4545
XmnI GAAnn'nnTTC
Cuts at: 3746 3746
Size: 5617
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI