Restriction map for
pFN21AA0175
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AocI
Aor51HI AscI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MaeII MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflII 1891 CTTAAG C^TTAAG
AhdI 1045 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 367 GTGCAC G^TGCAC
ApaI 1657 GGGCCC GGGCC^C
ApaLI 367 GTGCAC G^TGCAC
AseI 1110 ATTAAT AT^TAAT
AsnI 1110 ATTAAT AT^TAAT
AspEI 1045 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1505 GGTGCC G^G[CT][GA]CC
BanII 1657 GGGCCC G[GA]GC[CT]^C
BclI 930 TGATCA T^GATCA
BfrI 1891 CTTAAG C^TTAAG
BglI 964 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 1652 GAGACG CGTCTC(1/5)
BsmI 707 GCATTC GAATGC(1/-1)
BsrBI 1509 CCGCTC CCGCTC(-3/-3)
BstYI 1524 GGATCT [GA]^GATC[CT]
DraII 392 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1045 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 392 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 470 CCAAGG C^C[AT][AT]GG
FauI 1105 GCGGG CCCGC(4/6)
FbaI 930 TGATCA T^GATCA
HindIII 1672 AAGCTT A^AGCTT
MflI 1524 GGATCT [GA]^GATC[CT]
MspA1I 1885 CAGCGG C[AC]G^C[GT]G
NdeI 1540 CATATG CA^TATG
PflMI 1181 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 392 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1110 ATTAAT AT^TAAT
PstI 730 CTGCAG CTGCA^G
SfaNI 1900 GATGC GCATC(5/9)
SmaI 326 CCCGGG CCC^GGG
StuI 176 AGGCCT AGG^CCT
StyI 470 CCAAGG C^C[AT][AT]GG
TaqI 845 TCGA T^CGA
TthHB8I 845 TCGA T^CGA
Van91I 1181 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 87 CCATATCCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1524 GGATCT [GA]^GATC[CT]
XmaCI 326 CCCGGG C^CCGGG
XmaI 326 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 408 AAATTT [GA]^AATT[CT]
1452 AAATTC
ApoI 408 AAATTT [GA]^AATT[CT]
1452 AAATTC
AspHI 367 GTGCAC G[AT]GC[AT]^C
1618 GTGCTC
AvaI 326 CCCGGG C^[CT]CG[GA]G
974 CTCGGG
AvaII 393 GGACC G^G[AT]CC
738 GGACC
BsaBI 421 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
484 GATTACCATC
BsaI 322 GAGACC GGTCTC(1/5)
454 GGTCTC
BsiHKAI 367 GTGCAC G[AT]GC[AT]^C
1618 GTGCTC
BsmFI 52 GGGAC GGGAC(10/14)
392 GGGAC
BsoBI 326 CCCGGG C^[CT]CG[GA]G
974 CTCGGG
Bsp1407I 873 TGTACA T^GTACA
1767 TGTACA
BspMI 1245 ACCTGC ACCTGC(4/8)
732 GCAGGT
BsrGI 873 TGTACA T^GTACA
1767 TGTACA
BstF5I 778 CATCC GGATG(2/0)
1932 CATCC
Cac8I 82 GCCTGC GC[ACGT]^[ACGT]GC
1847 GCCAGC
EarI 316 GAAGAG CTCTTC(1/4)
1306 GAAGAG
FokI 778 CATCC GGATG(9/13)
1932 CATCC
HaeIII 177 GGCC GG^CC
1658 GGCC
HapII 156 CCGG C^CGG
327 CCGG
HincII 209 GTCAAC GT[CT]^[GA]AC
1759 GTTGAC
HindII 209 GTCAAC GT[CT]^[GA]AC
1759 GTTGAC
HpaII 156 CCGG C^CGG
327 CCGG
Ksp632I 316 GAAGAG CTCTTC(1/4)
1306 GAAGAG
MamI 421 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
484 GATTACCATC
MspI 156 CCGG C^CGG
327 CCGG
MwoI 517 GCTTATGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
964 GCCAAGAAGGC
NspI 499 ACATGC [GA]CATG^[CT]
1755 GCATGT
PshAI 448 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1479 GACAGGTGTC
SfcI 493 CTACAG C^T[GA][CT]AG
730 CTGCAG
SspBI 873 TGTACA T^GTACA
1767 TGTACA
SspI 26 AATATT AAT^ATT
243 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI MvaI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScaI ScrFI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI