(Circular) (Six-base) MAPSORT of: pFN21AA0175.seq Check: 1645 from: 1 to: 6658 pFN21AA0175 6658 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2728 3259 2728 Size: 531 6127 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4468 278 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4001 1937 Size: 2064 4594 Acc65I G'GTAC_C Cuts at: 3979 3979 Size: 6658 AclI AA'CG_TT Cuts at: 1563 4785 5158 1563 Size: 3222 373 3063 Fragments arranged by size: 3222 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3812 4721 6105 6373 1632 Size: 2180 909 1384 268 1917 Fragments arranged by size: 2180 1917 1384 909 268 AflII C'TTAA_G Cuts at: 820 1017 3896 820 Size: 197 2879 3582 Fragments arranged by size: 3582 2879 197 AflIII A'CryG_T Cuts at: 1203 5557 6534 1203 Size: 4354 977 1327 Fragments arranged by size: 4354 1327 977 AgeI A'CCGG_T Cuts at: 5489 5489 Size: 6658 AhdI GACnn_n'nnGTC Cuts at: 3055 5387 3055 Size: 2332 4326 AleI CACnn'nnGTG Cuts at: 1166 3793 1166 Size: 2627 4031 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6658 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2976 3008 2976 Size: 32 6626 AlwNI CAG_nnn'CTG Cuts at: 2534 3158 3427 4401 5973 2534 Size: 624 269 974 1572 3219 Fragments arranged by size: 3219 1572 974 624 269 ApaI G_GGCC'C Cuts at: 3666 3666 Size: 6658 ApaLI G'TGCA_C Cuts at: 2372 4715 5871 2372 Size: 2343 1156 3159 Fragments arranged by size: 3159 2343 1156 ApoI r'AATT_y Cuts at: 1493 2413 3457 3967 4193 4345 6454 1493 Size: 920 1044 510 226 152 2109 1697 Fragments arranged by size: 2109 1697 1044 920 510 226 152 AseI AT'TA_AT Cuts at: 160 1032 3116 5212 160 Size: 872 2084 2096 1606 Fragments arranged by size: 2096 2084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 AvaI C'yCGr_G Cuts at: 1870 1943 2331 2979 3983 1870 Size: 73 388 648 1004 4545 Fragments arranged by size: 4545 1004 648 388 73 BamHI G'GATC_C Cuts at: 3988 4355 3988 Size: 367 6291 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3510 3979 5434 618 Size: 325 217 627 1723 469 1455 1842 Fragments arranged by size: 1842 1723 1455 627 469 325 217 BanII G_rGCy'C Cuts at: 1391 3666 3977 1391 Size: 2275 311 4072 Fragments arranged by size: 4072 2275 311 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2332 3676 3939 928 Size: 971 433 1344 263 3647 Fragments arranged by size: 3647 1344 971 433 263 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4849 4883 1903 Size: 34 2912 34 3678 Fragments arranged by size: 3678 2912 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4550 5760 4550 Size: 1210 5448 BclI T'GATC_A Cuts at: 1548 2935 1548 Size: 1387 5271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2975 5269 136 Size: 107 122 71 631 605 1303 2294 1525 Fragments arranged by size: 2294 1525 1303 631 605 122 107 71 BglII A'GATC_T Cuts at: 6653 6653 Size: 6658 BlpI GC'TnA_GC Cuts at: 4075 4075 Size: 6658 Bme1580I G_kGCm'C Cuts at: 1790 2376 3666 4719 5875 1790 Size: 586 1290 1053 1156 2573 Fragments arranged by size: 2573 1290 1156 1053 586 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6658 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2700 4389 5347 Size: 416 53 83 399 24 1283 1689 958 Cuts at: 5347 442 Size: 1753 Fragments arranged by size: 1753 1689 1283 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6658 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3392 3392 Size: 6658 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2124 2295 3216 3327 5318 1239 Size: 885 171 921 111 1991 2579 Fragments arranged by size: 2579 1991 921 885 171 111 Bpu10I CC'TnA_GC Cuts at: 3330 3330 Size: 6658 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2282 2387 4783 5648 5946 2282 Size: 105 2396 865 298 2994 Fragments arranged by size: 2994 2396 865 298 105 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2321 2465 5321 882 Size: 617 822 144 2856 2219 Fragments arranged by size: 2856 2219 822 617 144 BsaAI yAC'GTr Cuts at: 493 1204 4449 493 Size: 711 3245 2702 Fragments arranged by size: 3245 2702 711 BsaBI GATnn'nnATC Cuts at: 2430 2493 6652 2430 Size: 63 4159 2436 Fragments arranged by size: 4159 2436 63 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4465 4847 5482 275 Size: 53 83 186 936 2932 382 635 1451 Fragments arranged by size: 2932 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5091 5489 5763 5910 6321 5091 Size: 398 274 147 411 5428 Fragments arranged by size: 5428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2097 2127 3564 3594 1178 Size: 30 165 30 694 30 1437 30 4242 Fragments arranged by size: 4242 1437 694 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2292 2339 3566 1186 Size: 1106 47 1227 4278 Fragments arranged by size: 4278 1227 1106 47 BseYI C'CCAG_C Cuts at: 5861 6357 5861 Size: 496 6162 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6085 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4108 4869 5897 1540 Size: 275 186 2107 761 1028 2301 Fragments arranged by size: 2301 2107 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2376 3627 4719 4804 5875 2376 Size: 1251 1092 85 1071 3159 Fragments arranged by size: 3159 1251 1092 1071 85 BsmI GAATG_Cn' Cuts at: 2712 4169 4262 2712 Size: 1457 93 5108 Fragments arranged by size: 5108 1457 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3651 3651 Size: 6658 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2376 3627 3666 3977 4719 4804 5875 Size: 399 586 1251 39 311 742 85 1071 Cuts at: 5875 1391 Size: 2174 Fragments arranged by size: 2174 1251 1071 742 586 399 311 85 39 BspEI T'CCGG_A Cuts at: 6321 6321 Size: 6658 BspHI T'CATG_A Cuts at: 4547 6277 4547 Size: 1730 4928 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2728 3259 4013 844 Size: 1884 531 754 3489 Fragments arranged by size: 3489 1884 754 531 BsrBI CCG'CTC Cuts at: 1394 1823 3516 4545 1394 Size: 429 1693 1029 3507 Fragments arranged by size: 3507 1693 1029 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5153 5327 65 Size: 1183 3905 174 1396 Fragments arranged by size: 3905 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5302 5489 1700 Size: 110 3492 187 2869 Fragments arranged by size: 3492 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2878 3772 96 Size: 2782 894 2982 Fragments arranged by size: 2982 2782 894 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6658 BssSI C'ACGA_G Cuts at: 4718 5730 4718 Size: 1012 5646 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6658 BstBI TT'CG_AA Cuts at: 5462 5462 Size: 6658 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6658 BstYI r'GATC_y Cuts at: 1972 3529 3988 4355 4740 4757 6209 6295 6307 Size: 1557 459 367 385 17 1452 86 12 Cuts at: 6307 6653 1972 Size: 346 1977 Fragments arranged by size: 1977 1557 1452 459 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3918 487 Size: 683 56 2692 3227 Fragments arranged by size: 3227 2692 683 56 BtsI GCAGTG_nn' Cuts at: 4174 4968 4988 4174 Size: 794 20 5844 Fragments arranged by size: 5844 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6658 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3192 3227 529 Size: 35 2628 35 3960 Fragments arranged by size: 3960 2628 35 35 DraI TTT'AAA Cuts at: 3962 3962 Size: 6658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5665 809 Size: 507 4349 1802 Fragments arranged by size: 4349 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4105 4994 6623 Size: 54 1425 50 275 18 2275 889 1629 Cuts at: 6623 8 Size: 43 Fragments arranged by size: 2275 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4105 1537 Size: 275 2293 4090 Fragments arranged by size: 4090 2293 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 3306 4588 1353 Size: 963 990 1282 3423 Fragments arranged by size: 3423 1282 990 963 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5245 5619 5765 387 Size: 1064 138 3656 374 146 1280 Fragments arranged by size: 3656 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2295 3216 3327 3812 4721 5318 Size: 393 492 171 921 111 485 909 597 Cuts at: 5318 6105 6373 1239 Size: 787 268 1524 Fragments arranged by size: 1524 921 909 787 597 492 485 393 268 171 111 EcoO109I rG'GnC_Cy Cuts at: 2398 2398 Size: 6658 EcoRI G'AATT_C Cuts at: 3967 4345 3967 Size: 378 6280 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3024 3024 Size: 6658 FspI TGC'GCA Cuts at: 5164 5164 Size: 6658 HaeII r_GCGC'y Cuts at: 1678 5805 1678 Size: 4127 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2300 2332 3041 3073 3131 Size: 32 135 32 728 32 709 32 58 Cuts at: 3131 3163 3926 3958 5299 5331 5373 5405 1373 Size: 32 763 32 1341 32 42 32 2626 Fragments arranged by size: 2626 1341 763 728 709 135 58 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2216 3766 4002 4248 669 Size: 988 281 278 1550 236 246 3079 Fragments arranged by size: 3079 1550 988 281 278 246 236 HindIII A'AGCT_T Cuts at: 748 3677 748 Size: 2929 3729 HpaI GTT'AAC Cuts at: 4248 4248 Size: 6658 KpnI G_GTAC'C Cuts at: 3983 3983 Size: 6658 MfeI C'AATT_G Cuts at: 4257 4257 Size: 6658 MluI A'CGCG_T Cuts at: 6534 6534 Size: 6658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3585 5772 5956 1305 Size: 2280 2187 184 2007 Fragments arranged by size: 2280 2187 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4836 Fragments arranged by size: 4836 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2850 3687 3793 4616 4975 5134 518 Size: 648 1684 837 106 823 359 159 2042 Fragments arranged by size: 2042 1684 837 823 648 359 159 106 MspA1I CmG'CkG Cuts at: 1606 3892 4074 4751 5899 6144 1606 Size: 2286 182 677 1148 245 2120 Fragments arranged by size: 2286 2120 1148 677 245 182 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6106 NdeI CA'TA_TG Cuts at: 387 3546 387 Size: 3159 3499 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6658 NotI GC'GGCC_GC Cuts at: 4105 4105 Size: 6658 NspI r_CATG'y Cuts at: 1132 1229 2508 3764 4016 5561 1132 Size: 97 1279 1256 252 1545 2229 Fragments arranged by size: 2229 1545 1279 1256 252 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6658 PciI A'CATG_T Cuts at: 5557 5557 Size: 6658 PflMI CCAn_nnn'nTGG Cuts at: 1108 3192 1108 Size: 2084 4574 PmeI GTTT'AAAC Cuts at: 3962 3962 Size: 6658 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4727 4759 6240 6272 4727 Size: 32 1481 32 5113 Fragments arranged by size: 5113 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2398 2398 Size: 6658 PshAI GACnn'nnGTC Cuts at: 1613 2457 3488 1613 Size: 844 1031 4783 Fragments arranged by size: 4783 1031 844 PsiI TTA'TAA Cuts at: 4228 4228 Size: 6658 PspOMI G'GGCC_C Cuts at: 3662 3662 Size: 6658 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2862 2894 2862 Size: 32 6626 PstI C_TGCA'G Cuts at: 830 2739 4010 830 Size: 1909 1271 3478 Fragments arranged by size: 3478 1909 1271 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6658 SalI G'TCGA_C Cuts at: 1936 4000 1936 Size: 2064 4594 SbfI CC_TGCA'GG Cuts at: 4010 4010 Size: 6658 ScaI AGT'ACT Cuts at: 2267 3191 3317 4906 6618 2267 Size: 924 126 1589 1712 2307 Fragments arranged by size: 2307 1712 1589 924 126 SfcI C'TryA_G Cuts at: 826 1045 2498 2735 4006 5141 5822 6013 826 Size: 219 1453 237 1271 1135 681 191 1471 Fragments arranged by size: 1471 1453 1271 1135 681 237 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6658 SmaI CCC'GGG Cuts at: 1872 2333 3985 1872 Size: 461 1652 4545 Fragments arranged by size: 4545 1652 461 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 2402 3896 4762 5663 5925 Size: 197 926 318 141 1494 866 901 262 Cuts at: 5925 820 Size: 1553 Fragments arranged by size: 1553 1494 926 901 866 318 262 197 141 SnaBI TAC'GTA Cuts at: 493 4449 493 Size: 3956 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6658 SphI G_CATG'C Cuts at: 1132 4016 1132 Size: 2884 3774 SspI AAT'ATT Cuts at: 5 52 2033 2250 4582 5 Size: 47 1981 217 2332 2081 Fragments arranged by size: 2332 2081 1981 217 47 StuI AGG'CCT Cuts at: 1517 2183 1517 Size: 666 5992 StyI C'CwwG_G Cuts at: 513 1065 2475 4097 513 Size: 552 1410 1622 3074 Fragments arranged by size: 3074 1622 1410 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4685 4702 4855 5040 5485 325 Size: 1255 3105 17 153 185 445 1498 Fragments arranged by size: 3105 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2265 2878 3189 Size: 274 80 33 51 1443 288 613 311 Cuts at: 3189 3315 3772 4904 6616 96 Size: 126 457 1132 1712 138 Fragments arranged by size: 1712 1443 1132 613 457 311 288 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3564 3596 6618 6650 3564 Size: 32 3022 32 3572 Fragments arranged by size: 3572 3022 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6658 XbaI T'CTAG_A Cuts at: 3994 3994 Size: 6658 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2099 2099 Size: 6658 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6658 XmaI C'CCGG_G Cuts at: 1870 2331 3983 1870 Size: 461 1652 4545 Fragments arranged by size: 4545 1652 461 XmnI GAAnn'nnTTC Cuts at: 4787 4787 Size: 6658 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4466 276 Size: 53 83 186 936 2932 2468 Fragments arranged by size: 2932 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI