Restriction map for
pFN21AA0175
(Linear) (Six-base) MAPSORT of: KIAA0175.seq Check: 6512 from: 1 to: 1953
KIAA0175.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 723 1254 1953
Size: 723 531 699
Fragments arranged by size:
723 699 531
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1807 1953
Size: 1807 146
AflII C'TTAA_G
Cuts at: 0 1891 1953
Size: 1891 62
AhdI GACnn_n'nnGTC
Cuts at: 0 1050 1953
Size: 1050 903
AleI CACnn'nnGTG
Cuts at: 0 1788 1953
Size: 1788 165
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 971 1003 1953
Size: 971 32 950
Fragments arranged by size:
971 950 32
AlwNI CAG_nnn'CTG
Cuts at: 0 529 1153 1422 1953
Size: 529 624 269 531
Fragments arranged by size:
624 531 529 269
ApaI G_GGCC'C
Cuts at: 0 1661 1953
Size: 1661 292
ApaLI G'TGCA_C
Cuts at: 0 367 1953
Size: 367 1586
ApoI r'AATT_y
Cuts at: 0 408 1452 1953
Size: 408 1044 501
Fragments arranged by size:
1044 501 408
AseI AT'TA_AT
Cuts at: 0 1111 1953
Size: 1111 842
AvaI C'yCGr_G
Cuts at: 0 326 974 1953
Size: 326 648 979
Fragments arranged by size:
979 648 326
BanI G'GyrC_C
Cuts at: 0 1505 1953
Size: 1505 448
BanII G_rGCy'C
Cuts at: 0 1661 1953
Size: 1661 292
BbsI GAAGACnn'nnnn_
Cuts at: 0 327 1671 1934 1953
Size: 327 1344 263 19
Fragments arranged by size:
1344 327 263 19
BclI T'GATC_A
Cuts at: 0 930 1953
Size: 930 1023
BglI GCCn_nnn'nGGC
Cuts at: 0 970 1953
Size: 970 983
Bme1580I G_kGCm'C
Cuts at: 0 371 1661 1953
Size: 371 1290 292
Fragments arranged by size:
1290 371 292
BmrI ACTGGGnnnn_n'
Cuts at: 0 695 1953
Size: 695 1258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1387 1953
Size: 1387 566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 290 1211 1322 1953
Size: 119 171 921 111 631
Fragments arranged by size:
921 631 171 119 111
Bpu10I CC'TnA_GC
Cuts at: 0 1325 1953
Size: 1325 628
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 382 1953
Size: 277 105 1571
Fragments arranged by size:
1571 277 105
BsaI GGTCTCn'nnnn_
Cuts at: 0 316 460 1953
Size: 316 144 1493
Fragments arranged by size:
1493 316 144
BsaBI GATnn'nnATC
Cuts at: 0 425 488 1953
Size: 425 63 1465
Fragments arranged by size:
1465 425 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 92 122 1559 1589 1953
Size: 92 30 1437 30 364
Fragments arranged by size:
1437 364 92 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 287 334 1561 1953
Size: 287 47 1227 392
Fragments arranged by size:
1227 392 287 47
BsiHKAI G_wGCw'C
Cuts at: 0 371 1622 1953
Size: 371 1251 331
Fragments arranged by size:
1251 371 331
BsmI GAATG_Cn'
Cuts at: 0 707 1953
Size: 707 1246
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1646 1953
Size: 1646 307
Bsp1286I G_dGCh'C
Cuts at: 0 371 1622 1661 1953
Size: 371 1251 39 292
Fragments arranged by size:
1251 371 292 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 723 1254 1953
Size: 723 531 699
Fragments arranged by size:
723 699 531
BsrBI CCG'CTC
Cuts at: 0 1511 1953
Size: 1511 442
BsrGI T'GTAC_A
Cuts at: 0 873 1767 1953
Size: 873 894 186
Fragments arranged by size:
894 873 186
BstYI r'GATC_y
Cuts at: 0 1524 1953
Size: 1524 429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1913 1953
Size: 1913 40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1187 1222 1953
Size: 1187 35 731
Fragments arranged by size:
1187 731 35
EarI CTCTTCn'nnn_
Cuts at: 0 311 1301 1953
Size: 311 990 652
Fragments arranged by size:
990 652 311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 290 1211 1322 1807 1953
Size: 119 171 921 111 485 146
Fragments arranged by size:
921 485 171 146 119 111
EcoO109I rG'GnC_Cy
Cuts at: 0 393 1953
Size: 393 1560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1019 1953
Size: 1019 934
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 1036 1068 1126 1158 1921 1953
Size: 295 32 709 32 58 32 763 32
Fragments arranged by size:
763 709 295 58 32 32 32 32
HincII GTy'rAC
Cuts at: 0 211 1761 1953
Size: 211 1550 192
Fragments arranged by size:
1550 211 192
HindIII A'AGCT_T
Cuts at: 0 1672 1953
Size: 1672 281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1580 1953
Size: 1580 373
MslI CAynn'nnrTG
Cuts at: 0 845 1682 1788 1953
Size: 845 837 106 165
Fragments arranged by size:
845 837 165 106
MspA1I CmG'CkG
Cuts at: 0 1887 1953
Size: 1887 66
NdeI CA'TA_TG
Cuts at: 0 1541 1953
Size: 1541 412
NspI r_CATG'y
Cuts at: 0 503 1759 1953
Size: 503 1256 194
Fragments arranged by size:
1256 503 194
PflMI CCAn_nnn'nTGG
Cuts at: 0 1187 1953
Size: 1187 766
PpuMI rG'GwC_Cy
Cuts at: 0 393 1953
Size: 393 1560
PshAI GACnn'nnGTC
Cuts at: 0 452 1483 1953
Size: 452 1031 470
Fragments arranged by size:
1031 470 452
PspOMI G'GGCC_C
Cuts at: 0 1657 1953
Size: 1657 296
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 857 889 1953
Size: 857 32 1064
Fragments arranged by size:
1064 857 32
PstI C_TGCA'G
Cuts at: 0 734 1953
Size: 734 1219
ScaI AGT'ACT
Cuts at: 0 262 1186 1312 1953
Size: 262 924 126 641
Fragments arranged by size:
924 641 262 126
SfcI C'TryA_G
Cuts at: 0 493 730 1953
Size: 493 237 1223
Fragments arranged by size:
1223 493 237
SmaI CCC'GGG
Cuts at: 0 328 1953
Size: 328 1625
SmlI C'TyrA_G
Cuts at: 0 256 397 1891 1953
Size: 256 141 1494 62
Fragments arranged by size:
1494 256 141 62
SspI AAT'ATT
Cuts at: 0 28 245 1953
Size: 28 217 1708
Fragments arranged by size:
1708 217 28
StuI AGG'CCT
Cuts at: 0 178 1953
Size: 178 1775
StyI C'CwwG_G
Cuts at: 0 470 1953
Size: 470 1483
TatI w'GTAC_w
Cuts at: 0 260 873 1184 1310 1767 1953
Size: 260 613 311 126 457 186
Fragments arranged by size:
613 457 311 260 186 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1559 1591 1953
Size: 1559 32 362
Fragments arranged by size:
1559 362 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 94 1953
Size: 94 1859
XmaI C'CCGG_G
Cuts at: 0 326 1953
Size: 326 1627
Enzymes that do cut:
AarI AcuI AflII AhdI AleI AloI AlwNI ApaI
ApaLI ApoI AseI AvaI BanI BanII BbsI BclI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaXI BseRI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrGI BstYI BtgZI CspCI EarI Eco57MI EcoO109I
FalI Hin4I HincII HindIII MmeI MslI MspA1I NdeI
NspI PflMI PpuMI PshAI PspOMI PsrI PstI ScaI
SfcI SmaI SmlI SspI StuI StyI TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AlfI
AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaHI
BsaWI BseYI BsgI BsiEI BsiWI BspEI BspHI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtsI ClaI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PsiI
PspXI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI TaqII Tth111I XbaI XhoI
XmnI ZraI