(Circular) (Six-base) MAPSORT of: pFN21AB7822.seq Check: 3918 from: 1 to: 6505 pFN21AB7822 6505 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3220 3220 Size: 6505 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2184 4315 278 Size: 53 83 186 936 648 2131 2468 Fragments arranged by size: 2468 2131 936 648 186 83 53 AccI GT'mk_AC Cuts at: 1937 3848 1937 Size: 1911 4594 Acc65I G'GTAC_C Cuts at: 3192 3826 3192 Size: 634 5871 AclI AA'CG_TT Cuts at: 1563 4632 5005 1563 Size: 3069 373 3063 Fragments arranged by size: 3069 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2405 3569 4568 5952 6220 1632 Size: 773 1164 999 1384 268 1917 Fragments arranged by size: 1917 1384 1164 999 773 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6308 AflIII A'CryG_T Cuts at: 1203 5404 6381 1203 Size: 4201 977 1327 Fragments arranged by size: 4201 1327 977 AgeI A'CCGG_T Cuts at: 5336 5336 Size: 6505 AhdI GACnn_n'nnGTC Cuts at: 5234 5234 Size: 6505 AleI CACnn'nnGTG Cuts at: 1166 2936 1166 Size: 1770 4735 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2350 1184 Size: 1166 5339 AlwNI CAG_nnn'CTG Cuts at: 2410 2425 2761 3055 4248 5820 2410 Size: 15 336 294 1193 1572 3095 Fragments arranged by size: 3095 1572 1193 336 294 15 ApaLI G'TGCA_C Cuts at: 3350 4562 5718 3350 Size: 1212 1156 4137 Fragments arranged by size: 4137 1212 1156 ApoI r'AATT_y Cuts at: 1493 3814 4040 4192 6301 1493 Size: 2321 226 152 2109 1697 Fragments arranged by size: 2321 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5059 160 Size: 872 4027 1606 Fragments arranged by size: 4027 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6505 AvaI C'yCGr_G Cuts at: 1870 1943 2414 2701 2990 3108 3201 3636 3830 Size: 73 471 287 289 118 93 435 194 Cuts at: 3830 1870 Size: 4545 Fragments arranged by size: 4545 471 435 289 287 194 118 93 73 BamHI G'GATC_C Cuts at: 3835 4202 3835 Size: 367 6138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2938 3192 3598 3826 5281 Size: 325 217 627 1151 254 406 228 1455 Cuts at: 5281 618 Size: 1842 Fragments arranged by size: 1842 1455 1151 627 406 325 254 228 217 BanII G_rGCy'C Cuts at: 1391 2211 2852 2891 3099 3170 3416 3526 3824 Size: 820 641 39 208 71 246 110 298 Cuts at: 3824 1391 Size: 4072 Fragments arranged by size: 4072 820 641 298 246 208 110 71 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5534 BbvCI CC'TCA_GC Cuts at: 3237 3237 Size: 6505 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3730 3764 4696 4730 1903 Size: 34 1793 34 932 34 3678 Fragments arranged by size: 3678 1793 932 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4397 5607 4397 Size: 1210 5295 BclI T'GATC_A Cuts at: 1548 1548 Size: 6505 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2219 2619 5116 Size: 107 122 71 631 605 547 400 2497 Cuts at: 5116 136 Size: 1525 Fragments arranged by size: 2497 1525 631 605 547 400 122 107 71 BglII A'GATC_T Cuts at: 6500 6500 Size: 6505 BlpI GC'TnA_GC Cuts at: 3922 3922 Size: 6505 Bme1580I G_kGCm'C Cuts at: 1790 2898 3354 4566 5722 1790 Size: 1108 456 1212 1156 2573 Fragments arranged by size: 2573 1212 1156 1108 456 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6505 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2997 3011 4236 Size: 416 53 83 399 24 1580 14 1225 Cuts at: 4236 5194 442 Size: 958 1753 Fragments arranged by size: 1753 1580 1225 958 416 399 83 53 24 14 BmtI G_CTAG'C Cuts at: 1056 2511 1056 Size: 1455 5050 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2138 2387 2651 3392 5165 1239 Size: 856 43 249 264 741 1773 2579 Fragments arranged by size: 2579 1773 856 741 264 249 43 Bpu10I CC'TnA_GC Cuts at: 3237 3473 3237 Size: 236 6269 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 3050 4630 5495 5793 2168 Size: 882 1580 865 298 2880 Fragments arranged by size: 2880 1580 882 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2503 5168 882 Size: 617 1004 2665 2219 Fragments arranged by size: 2665 2219 1004 617 BsaAI yAC'GTr Cuts at: 493 1204 4296 493 Size: 711 3092 2702 Fragments arranged by size: 3092 2702 711 BsaBI GATnn'nnATC Cuts at: 6499 6499 Size: 6505 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 2181 3246 4312 Size: 53 83 186 936 488 160 1065 1066 Cuts at: 4312 4694 5329 275 Size: 382 635 1451 Fragments arranged by size: 1451 1066 1065 936 635 488 382 186 160 83 53 BsaWI w'CCGG_w Cuts at: 2079 3258 4938 5336 5610 5757 6168 2079 Size: 1179 1680 398 274 147 411 2416 Fragments arranged by size: 2416 1680 1179 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1999 2029 2681 2711 3350 Size: 30 165 30 596 30 652 30 639 Cuts at: 3350 3380 1178 Size: 30 4303 Fragments arranged by size: 4303 652 639 596 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 3121 3148 3572 1186 Size: 1033 902 27 424 4119 Fragments arranged by size: 4119 1033 902 424 27 BseYI C'CCAG_C Cuts at: 2434 2897 3088 5708 6204 2434 Size: 463 191 2620 496 2735 Fragments arranged by size: 2735 2620 496 463 191 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5932 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2342 2687 3955 4716 5744 1540 Size: 275 186 341 345 1268 761 1028 2301 Fragments arranged by size: 2301 1268 1028 761 345 341 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 3354 3566 4566 4651 5722 2211 Size: 1143 212 1000 85 1071 2994 Fragments arranged by size: 2994 1143 1071 1000 212 85 BsiWI C'GTAC_G Cuts at: 2980 2980 Size: 6505 BsmI GAATG_Cn' Cuts at: 4016 4109 4016 Size: 93 6412 BsmBI CGTCTCn'nnnn_ Cuts at: 2028 2028 Size: 6505 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2852 2891 2898 3099 3170 3354 Size: 399 421 641 39 7 201 71 184 Cuts at: 3354 3416 3526 3566 3824 4566 4651 5722 1391 Size: 62 110 40 258 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 641 421 399 258 201 184 110 85 71 62 40 39 7 BspEI T'CCGG_A Cuts at: 2079 6168 2079 Size: 4089 2416 BspHI T'CATG_A Cuts at: 4394 6124 4394 Size: 1730 4775 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3220 3860 844 Size: 2376 640 3489 Fragments arranged by size: 3489 2376 640 BsrBI CCG'CTC Cuts at: 1394 1823 3271 4392 1394 Size: 429 1448 1121 3507 Fragments arranged by size: 3507 1448 1121 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2401 5000 5174 65 Size: 1183 1153 2599 174 1396 Fragments arranged by size: 2599 1396 1183 1153 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2043 2260 2306 2337 3477 5149 5336 Size: 110 233 217 46 31 1140 1672 187 Cuts at: 5336 1700 Size: 2869 Fragments arranged by size: 2869 1672 1140 233 217 187 110 46 31 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6505 BssHII G'CGCG_C Cuts at: 1925 3507 1925 Size: 1582 4923 BssSI C'ACGA_G Cuts at: 4565 5577 4565 Size: 1012 5493 BstAPI GCAn_nnn'nTGC Cuts at: 794 2927 794 Size: 2133 4372 BstBI TT'CG_AA Cuts at: 3420 5309 3420 Size: 1889 4616 BstEII G'GTnAC_C Cuts at: 1184 2456 1184 Size: 1272 5233 BstXI CCAn_nnnn'nTGG Cuts at: 2590 3024 2590 Size: 434 6071 BstYI r'GATC_y Cuts at: 1972 2664 3835 4202 4587 4604 6056 6142 6154 Size: 692 1171 367 385 17 1452 86 12 Cuts at: 6154 6500 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1171 692 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3160 3160 Size: 6505 BtgI C'CryG_G Cuts at: 513 1065 2909 3615 513 Size: 552 1844 706 3403 Fragments arranged by size: 3403 1844 706 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5766 Fragments arranged by size: 5766 683 56 BtsI GCAGTG_nn' Cuts at: 2605 3743 4021 4815 4835 2605 Size: 1138 278 794 20 4275 Fragments arranged by size: 4275 1138 794 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6505 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2638 2673 529 Size: 35 2074 35 4361 Fragments arranged by size: 4361 2074 35 35 DraI TTT'AAA Cuts at: 3809 3809 Size: 6505 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2522 5512 809 Size: 507 1206 2990 1802 Fragments arranged by size: 2990 1802 1206 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2339 2473 3683 Size: 54 1425 50 275 18 509 134 1210 Cuts at: 3683 3952 4841 6470 8 Size: 269 889 1629 43 Fragments arranged by size: 1629 1425 1210 889 509 275 269 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2339 3952 1537 Size: 275 527 1613 4090 Fragments arranged by size: 4090 1613 527 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2381 3182 4435 1353 Size: 1028 801 1253 3423 Fragments arranged by size: 3423 1253 1028 801 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3155 5092 5466 5612 387 Size: 1064 138 1566 1937 374 146 1280 Fragments arranged by size: 1937 1566 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2209 2209 Size: 6505 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2138 2387 2405 2651 3392 3569 Size: 393 463 43 249 18 246 741 177 Cuts at: 3569 4568 5165 5952 6220 1239 Size: 999 597 787 268 1524 Fragments arranged by size: 1524 999 787 741 597 463 393 268 249 246 177 43 18 EcoNI CCTnn'n_nnAGG Cuts at: 3551 3551 Size: 6505 EcoO109I rG'GnC_Cy Cuts at: 2786 2804 2786 Size: 18 6487 EcoRI G'AATT_C Cuts at: 3814 4192 3814 Size: 378 6127 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2162 3298 2162 Size: 1136 5369 FspI TGC'GCA Cuts at: 5011 5011 Size: 6505 HaeII r_GCGC'y Cuts at: 1678 2285 2452 3775 5652 1678 Size: 607 167 1323 1877 2531 Fragments arranged by size: 2531 1877 1323 607 167 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 3140 3172 3295 Size: 32 135 32 427 32 1109 32 123 Cuts at: 3295 3327 3350 3382 5146 5178 5220 5252 1373 Size: 32 23 32 1764 32 42 32 2626 Fragments arranged by size: 2626 1764 1109 427 135 123 42 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 2674 3217 3849 4095 669 Size: 988 281 736 543 632 246 3079 Fragments arranged by size: 3079 988 736 632 543 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6505 HpaI GTT'AAC Cuts at: 3217 4095 3217 Size: 878 5627 KpnI G_GTAC'C Cuts at: 3196 3830 3196 Size: 634 5871 MfeI C'AATT_G Cuts at: 4104 4104 Size: 6505 MluI A'CGCG_T Cuts at: 6381 6381 Size: 6505 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5619 5803 1305 Size: 4314 184 2007 Fragments arranged by size: 4314 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4683 Fragments arranged by size: 4683 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2271 2936 4463 4822 4981 518 Size: 648 1105 665 1527 359 159 2042 Fragments arranged by size: 2042 1527 1105 665 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2422 2761 2911 3052 3586 3921 4598 Size: 406 410 339 150 141 534 335 677 Cuts at: 4598 5746 5991 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 534 410 406 339 335 245 150 141 NaeI GCC'GGC Cuts at: 2262 2339 3479 2262 Size: 77 1140 5288 Fragments arranged by size: 5288 1140 77 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5953 NdeI CA'TA_TG Cuts at: 387 387 Size: 6505 NgoMIV G'CCGG_C Cuts at: 2260 2337 3477 2260 Size: 77 1140 5288 Fragments arranged by size: 5288 1140 77 NheI G'CTAG_C Cuts at: 1052 2507 1052 Size: 1455 5050 NotI GC'GGCC_GC Cuts at: 3952 3952 Size: 6505 NspI r_CATG'y Cuts at: 1132 1229 2609 3593 3863 5408 1132 Size: 97 1380 984 270 1545 2229 Fragments arranged by size: 2229 1545 1380 984 270 97 PasI CC'CwG_GG Cuts at: 1348 2480 3518 1348 Size: 1132 1038 4335 Fragments arranged by size: 4335 1132 1038 PciI A'CATG_T Cuts at: 5404 5404 Size: 6505 PflMI CCAn_nnn'nTGG Cuts at: 1108 3055 3681 1108 Size: 1947 626 3932 Fragments arranged by size: 3932 1947 626 PfoI T'CCnGG_A Cuts at: 3368 3368 Size: 6505 PmeI GTTT'AAAC Cuts at: 3809 3809 Size: 6505 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4574 4606 6087 6119 4574 Size: 32 1481 32 4960 Fragments arranged by size: 4960 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2804 2804 Size: 6505 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6505 PsiI TTA'TAA Cuts at: 4075 4075 Size: 6505 PspXI vC'TCGA_Gb Cuts at: 2990 2990 Size: 6505 PstI C_TGCA'G Cuts at: 830 2055 2757 3857 830 Size: 1225 702 1100 3478 Fragments arranged by size: 3478 1225 1100 702 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6505 PvuII CAG'CTG Cuts at: 2761 3586 2761 Size: 825 5680 SacI G_AGCT'C Cuts at: 2211 2211 Size: 6505 SacII CC_GC'GG Cuts at: 2912 2912 Size: 6505 SalI G'TCGA_C Cuts at: 1936 3847 1936 Size: 1911 4594 SanDI GG'GwC_CC Cuts at: 2804 2804 Size: 6505 SapI GCTCTTCn'nnn_ Cuts at: 2381 2381 Size: 6505 SbfI CC_TGCA'GG Cuts at: 3857 3857 Size: 6505 ScaI AGT'ACT Cuts at: 4753 6465 4753 Size: 1712 4793 SfcI C'TryA_G Cuts at: 826 1045 2051 2753 3853 4988 5669 5860 826 Size: 219 1006 702 1100 1135 681 191 1471 Fragments arranged by size: 1471 1135 1100 1006 702 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6505 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6505 SmaI CCC'GGG Cuts at: 1872 2416 2703 3110 3638 3832 1872 Size: 544 287 407 528 194 4545 Fragments arranged by size: 4545 544 528 407 287 194 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 2990 3029 3201 4609 5510 Size: 197 926 204 843 39 172 1408 901 Cuts at: 5510 5772 820 Size: 262 1553 Fragments arranged by size: 1553 1408 926 901 843 262 204 197 172 39 SnaBI TAC'GTA Cuts at: 493 4296 493 Size: 3803 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6505 SphI G_CATG'C Cuts at: 1132 2609 3863 1132 Size: 1477 1254 3774 Fragments arranged by size: 3774 1477 1254 SrfI GCCC'GGGC Cuts at: 2416 2416 Size: 6505 SspI AAT'ATT Cuts at: 5 52 4429 5 Size: 47 4377 2081 Fragments arranged by size: 4377 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6505 StyI C'CwwG_G Cuts at: 513 1065 2493 2799 2883 3135 3396 3944 513 Size: 552 1428 306 84 252 261 548 3074 Fragments arranged by size: 3074 1428 552 548 306 261 252 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2380 2493 2506 2896 4532 4549 4702 Size: 1255 800 113 13 390 1636 17 153 Cuts at: 4702 4887 5332 325 Size: 185 445 1498 Fragments arranged by size: 1636 1498 1255 800 445 390 185 153 113 17 13 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4751 6463 96 Size: 274 80 33 51 1443 2774 1712 138 Fragments arranged by size: 2774 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2681 2713 6465 6497 2681 Size: 32 3752 32 2689 Fragments arranged by size: 3752 2689 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6505 XbaI T'CTAG_A Cuts at: 3841 3841 Size: 6505 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3376 3376 Size: 6505 XhoI C'TCGA_G Cuts at: 1943 2990 3201 1943 Size: 1047 211 5247 Fragments arranged by size: 5247 1047 211 XmaI C'CCGG_G Cuts at: 1870 2414 2701 3108 3636 3830 1870 Size: 544 287 407 528 194 4545 Fragments arranged by size: 4545 544 528 407 287 194 XmnI GAAnn'nnTTC Cuts at: 4634 4634 Size: 6505 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2182 4313 276 Size: 53 83 186 936 648 2131 2468 Fragments arranged by size: 2468 2131 936 648 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SexAI SfiI SfoI SwaI