################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## Acc65I AclI AcsI AflII AflIII AgeI AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsiEI BsiWI Bsp1407I BspLU11I BspMI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 1753 GACGTC GACGT^C AccI 384 GTCTAC GT^[AC][GT]AC AccII 1907 CGCG CG^CG AccIII 770 TCCGGA T^CCGGA AcyI 1753 GACGTC G[GA]^CG[CT]C Alw44I 1936 GTGCAC G^TGCAC Aor51HI 1281 AGCGCT AGC^GCT ApaLI 1936 GTGCAC G^TGCAC AspI 954 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1763 CTCGGG C^[CT]CG[GA]G BanI 253 GGCACC G^G[CT][GA]CC BsaHI 1753 GACGTC G[GA]^CG[CT]C BseAI 770 TCCGGA T^CCGGA BsmBI 64 CGTCTC CGTCTC(1/5) BsoBI 1763 CTCGGG C^[CT]CG[GA]G BspDI 1735 ATCGAT AT^CGAT BspEI 770 TCCGGA T^CCGGA BssSI 316 CACGAG CACGAG(-5/-1) BstUI 1907 CGCG CG^CG ClaI 1735 ATCGAT AT^CGAT Eco47III 1281 AGCGCT AGC^GCT Hin1I 1753 GACGTC G[GA]^CG[CT]C HincII 322 GTCAAC GT[CT]^[GA]AC HindII 322 GTCAAC GT[CT]^[GA]AC MroI 770 TCCGGA T^CCGGA MvnI 1907 CGCG CG^CG NaeI 1543 GCCGGC GCC^GGC NgoMIV 1543 GCCGGC G^CCGGC NspI 1247 GCATGC [GA]CATG^[CT] PflFI 954 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1776 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 357 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 278 AGTACT AGT^ACT SphI 1247 GCATGC GCATG^C Tsp509I 107 AATT ^AATT Tth111I 954 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1776 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AfaI 279 GTAC GT^AC 1302 GTAC AlwNI 1651 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1777 CAGAGGCTG BbsI 1470 GAAGAC GAAGAC(2/6) 1642 GAAGAC BcgI 1266 CGAATCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1727 GCATCCACATCG BciVI 612 GTATCC GTATCC(6/5) 1018 GTATCC BfaI 2071 CTAG C^TAG 2395 CTAG BlpI 885 GCTGAGC GC^T[ACGT]AGC 1899 GCTGAGC Bpu1102I 885 GCTGAGC GC^T[ACGT]AGC 1899 GCTGAGC BpuAI 1470 GAAGAC GAAGAC(2/6) 1642 GAAGAC BsaWI 722 ACCGGA [AT]^CCGG[AT] 770 TCCGGA BsmI 741 GAATGC GAATGC(1/-1) 1482 GAATGC BsrBI 757 GAGCGG CCGCTC(-3/-3) 1385 GAGCGG BsrFI 764 ACCGGC [GA]^CCGG[CT] 1543 GCCGGC BstAPI 438 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1980 GCAGCGGATGC BstXI 1022 CCAGCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1205 CCATAACCTTGG BstYI 1105 GGATCT [GA]^GATC[CT] 1785 GGATCT CelII 885 GCTGAGC GC^T[ACGT]AGC 1899 GCTGAGC CfoI 1282 GCGC GCG^C 1342 GCGC Cfr10I 764 ACCGGC [GA]^CCGG[CT] 1543 GCCGGC HaeII 1281 AGCGCT [GA]GCGC^[CT] 1341 GGCGCT HgaI 174 GACGC GACGC(5/10) 212 GCGTC HhaI 1282 GCGC GCG^C 1342 GCGC HinP1I 1282 GCGC G^CGC 1342 GCGC MaeI 2071 CTAG C^TAG 2395 CTAG MflI 1105 GGATCT [GA]^GATC[CT] 1785 GGATCT NcoI 389 CCATGG C^CATGG 2339 CCATGG RsaI 279 GTAC GT^AC 1302 GTAC SapI 483 GAAGAGC GCTCTTC(1/4) 754 GAAGAGC SexAI 1931 ACCTGGT A^CC[AT]GGT 2264 ACCTGGT StuI 1493 AGGCCT AGG^CCT 1925 AGGCCT TfiI 964 GAATC G^A[AT]TC 1267 GAATC Tsp45I 419 GTGAC ^GT[GC]AC 557 GTGAC XcmI 1709 CCATGCTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2255 CCAACAGCAACCTGG XhoII 1105 GGATCT [GA]^GATC[CT] 1785 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AluI AlwI AspHI AvaII BalI BanII BbvCI BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RcaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI TspRI TthHB8I