(Circular) (Six-base) MAPSORT of: pFN21AB6432.seq Check: 7348 from: 1 to: 5737 pFN21AB6432 5737 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3547 278 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3080 1937 Size: 1143 4594 Acc65I G'GTAC_C Cuts at: 3058 3058 Size: 5737 AclI AA'CG_TT Cuts at: 1563 3864 4237 1563 Size: 2301 373 3063 Fragments arranged by size: 3063 2301 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2255 2703 3800 5184 5452 1632 Size: 623 448 1097 1384 268 1917 Fragments arranged by size: 1917 1384 1097 623 448 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5540 AflIII A'CryG_T Cuts at: 1203 4636 5613 1203 Size: 3433 977 1327 Fragments arranged by size: 3433 1327 977 AgeI A'CCGG_T Cuts at: 4568 4568 Size: 5737 AhdI GACnn_n'nnGTC Cuts at: 2796 4466 2796 Size: 1670 4067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5737 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5737 AlwNI CAG_nnn'CTG Cuts at: 3480 5052 3480 Size: 1572 4165 ApaLI G'TGCA_C Cuts at: 2223 3794 4950 2223 Size: 1571 1156 3010 Fragments arranged by size: 3010 1571 1156 ApoI r'AATT_y Cuts at: 1493 2357 2546 2688 3046 3272 3424 5533 1493 Size: 864 189 142 358 226 152 2109 1697 Fragments arranged by size: 2109 1697 864 358 226 189 152 142 AseI AT'TA_AT Cuts at: 160 1032 4291 160 Size: 872 3259 1606 Fragments arranged by size: 3259 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2082 3062 1870 Size: 73 89 50 980 4545 Fragments arranged by size: 4545 980 89 73 50 BamHI G'GATC_C Cuts at: 3067 3434 3067 Size: 367 5370 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3058 4513 618 Size: 325 217 627 1271 1455 1842 Fragments arranged by size: 1842 1455 1271 627 325 217 BanII G_rGCy'C Cuts at: 1391 3056 1391 Size: 1665 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4766 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3928 3962 1903 Size: 34 1991 34 3678 Fragments arranged by size: 3678 1991 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3629 4839 3629 Size: 1210 4527 BclI T'GATC_A Cuts at: 1548 1548 Size: 5737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4348 136 Size: 107 122 71 631 605 2676 1525 Fragments arranged by size: 2676 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5732 5732 Size: 5737 BlpI GC'TnA_GC Cuts at: 3154 3154 Size: 5737 Bme1580I G_kGCm'C Cuts at: 1790 2227 2974 3798 4954 1790 Size: 437 747 824 1156 2573 Fragments arranged by size: 2573 1156 824 747 437 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5737 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3468 4426 442 Size: 416 53 83 399 24 2051 958 1753 Fragments arranged by size: 2051 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5737 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2108 Size: 5737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2168 4397 1239 Size: 836 93 2229 2579 Fragments arranged by size: 2579 2229 836 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2858 3862 4727 5025 2858 Size: 1004 865 298 3570 Fragments arranged by size: 3570 1004 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4400 882 Size: 617 2901 2219 Fragments arranged by size: 2901 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2635 3528 493 Size: 711 1431 893 2702 Fragments arranged by size: 2702 1431 893 711 BsaBI GATnn'nnATC Cuts at: 2568 5731 2568 Size: 3163 2574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3544 3926 4561 275 Size: 53 83 186 936 2011 382 635 1451 Fragments arranged by size: 2011 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4170 4568 4842 4989 5400 4170 Size: 398 274 147 411 4507 Fragments arranged by size: 4507 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2970 3000 1178 Size: 30 165 30 1567 30 3915 Fragments arranged by size: 3915 1567 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 2978 1186 Size: 1117 675 3945 Fragments arranged by size: 3945 1117 675 BseYI C'CCAG_C Cuts at: 4940 5436 4940 Size: 496 5241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5164 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3187 3948 4976 1540 Size: 275 186 1186 761 1028 2301 Fragments arranged by size: 2301 1186 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 2227 3798 3883 4954 2094 Size: 133 1571 85 1071 2877 Fragments arranged by size: 2877 1571 1071 133 85 BsmI GAATG_Cn' Cuts at: 2198 3248 3341 2198 Size: 1050 93 4594 Fragments arranged by size: 4594 1050 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2227 2974 3056 3798 3883 4954 Size: 399 304 133 747 82 742 85 1071 Cuts at: 4954 1391 Size: 2174 Fragments arranged by size: 2174 1071 747 742 399 304 133 85 82 BspEI T'CCGG_A Cuts at: 5400 5400 Size: 5737 BspHI T'CATG_A Cuts at: 3626 5356 3626 Size: 1730 4007 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3092 844 Size: 2248 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3624 1394 Size: 429 1801 3507 Fragments arranged by size: 3507 1801 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2210 2560 4232 4406 65 Size: 1183 962 350 1672 174 1396 Fragments arranged by size: 1672 1396 1183 962 350 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4381 4568 1700 Size: 110 2571 187 2869 Fragments arranged by size: 2869 2571 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5737 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5737 BssSI C'ACGA_G Cuts at: 3797 4809 3797 Size: 1012 4725 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5737 BstBI TT'CG_AA Cuts at: 4541 4541 Size: 5737 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5737 BstYI r'GATC_y Cuts at: 1972 2430 3067 3434 3819 3836 5288 5374 5386 Size: 458 637 367 385 17 1452 86 12 Cuts at: 5386 5732 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 637 458 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 3015 513 Size: 552 939 12 999 3235 Fragments arranged by size: 3235 999 939 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4998 Fragments arranged by size: 4998 683 56 BtsI GCAGTG_nn' Cuts at: 3253 4047 4067 3253 Size: 794 20 4923 Fragments arranged by size: 4923 794 20 ClaI AT'CG_AT Cuts at: 1554 2169 1554 Size: 615 5122 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5702 DraI TTT'AAA Cuts at: 2626 3041 2626 Size: 415 5322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4744 809 Size: 507 3428 1802 Fragments arranged by size: 3428 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3184 4073 5702 Size: 54 1425 50 275 18 1354 889 1629 Cuts at: 5702 8 Size: 43 Fragments arranged by size: 1629 1425 1354 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3184 1537 Size: 275 1372 4090 Fragments arranged by size: 4090 1372 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2304 3667 1353 Size: 951 1363 3423 Fragments arranged by size: 3423 1363 951 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4324 4698 4844 387 Size: 1064 138 2735 374 146 1280 Fragments arranged by size: 2735 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2168 2255 2703 3800 4397 5184 Size: 393 443 93 87 448 1097 597 787 Cuts at: 5184 5452 1239 Size: 268 1524 Fragments arranged by size: 1524 1097 787 597 448 443 393 268 93 87 EcoNI CCTnn'n_nnAGG Cuts at: 2378 2378 Size: 5737 EcoRI G'AATT_C Cuts at: 2688 3046 3424 2688 Size: 358 378 5001 Fragments arranged by size: 5001 378 358 FspI TGC'GCA Cuts at: 4243 4243 Size: 5737 HaeII r_GCGC'y Cuts at: 1678 4884 1678 Size: 3206 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2782 2814 4378 4410 4452 Size: 32 135 32 1210 32 1564 32 42 Cuts at: 4452 4484 1373 Size: 32 2626 Fragments arranged by size: 2626 1564 1210 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3327 669 Size: 988 281 1143 246 3079 Fragments arranged by size: 3079 1143 988 281 246 HindIII A'AGCT_T Cuts at: 748 2893 748 Size: 2145 3592 HpaI GTT'AAC Cuts at: 3327 3327 Size: 5737 KpnI G_GTAC'C Cuts at: 3062 3062 Size: 5737 MfeI C'AATT_G Cuts at: 3336 3336 Size: 5737 MluI A'CGCG_T Cuts at: 5613 5613 Size: 5737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2107 4851 5035 1305 Size: 802 2744 184 2007 Fragments arranged by size: 2744 2007 802 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3915 Fragments arranged by size: 3915 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3695 4054 4213 518 Size: 648 2529 359 159 2042 Fragments arranged by size: 2529 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2040 3153 3830 4978 5223 1606 Size: 434 1113 677 1148 245 2120 Fragments arranged by size: 2120 1148 1113 677 434 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3015 513 Size: 552 939 1011 3235 Fragments arranged by size: 3235 1011 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5737 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5737 NotI GC'GGCC_GC Cuts at: 3184 3184 Size: 5737 NspI r_CATG'y Cuts at: 1132 1229 2722 3095 4640 1132 Size: 97 1493 373 1545 2229 Fragments arranged by size: 2229 1545 1493 373 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5737 PciI A'CATG_T Cuts at: 4636 4636 Size: 5737 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5737 PmeI GTTT'AAAC Cuts at: 3041 3041 Size: 5737 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3806 3838 5319 5351 3806 Size: 32 1481 32 4192 Fragments arranged by size: 4192 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5737 PsiI TTA'TAA Cuts at: 3307 3307 Size: 5737 PstI C_TGCA'G Cuts at: 830 3089 830 Size: 2259 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5737 SalI G'TCGA_C Cuts at: 1936 3079 1936 Size: 1143 4594 SbfI CC_TGCA'GG Cuts at: 3089 3089 Size: 5737 ScaI AGT'ACT Cuts at: 3985 5697 3985 Size: 1712 4025 SfcI C'TryA_G Cuts at: 826 1045 2745 3085 4220 4901 5092 826 Size: 219 1700 340 1135 681 191 1471 Fragments arranged by size: 1700 1471 1135 681 340 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5737 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5737 SmaI CCC'GGG Cuts at: 1872 3064 1872 Size: 1192 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2837 3841 4742 5004 820 Size: 197 926 894 1004 901 262 1553 Fragments arranged by size: 1553 1004 926 901 894 262 197 SnaBI TAC'GTA Cuts at: 493 3528 493 Size: 3035 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5737 SphI G_CATG'C Cuts at: 1132 2722 3095 1132 Size: 1590 373 3774 Fragments arranged by size: 3774 1590 373 SspI AAT'ATT Cuts at: 5 52 2767 2879 3661 5 Size: 47 2715 112 782 2081 Fragments arranged by size: 2715 2081 782 112 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5737 StyI C'CwwG_G Cuts at: 513 1065 2004 3015 3176 513 Size: 552 939 1011 161 3074 Fragments arranged by size: 3074 1011 939 552 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3764 3781 3934 4119 4564 325 Size: 1255 2184 17 153 185 445 1498 Fragments arranged by size: 2184 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3983 5695 96 Size: 274 80 33 51 1443 2006 1712 138 Fragments arranged by size: 2006 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5697 5729 5697 Size: 32 5705 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5737 XbaI T'CTAG_A Cuts at: 2645 3073 2645 Size: 428 5309 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5737 XmaI C'CCGG_G Cuts at: 1870 3062 1870 Size: 1192 4545 XmnI GAAnn'nnTTC Cuts at: 2103 2451 2688 2880 3866 2103 Size: 348 237 192 986 3974 Fragments arranged by size: 3974 986 348 237 192 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3545 276 Size: 53 83 186 936 2011 2468 Fragments arranged by size: 2468 2011 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI