Restriction map for
pFN21AA1461
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI Aor51HI ApaI AscI AviII
AvrII BamHI BbeI BbrPI BciVI
BfrI BlnI BsaAI BsiEI BsiWI
BsmBI BspDI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstUI BstZ17I
CfoI ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KspI MluI MvnI NarI
NdeI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 589 GTCTAC GT^[AC][GT]AC
AccIII 4319 TCCGGA T^CCGGA
AcyI 2602 GGCGTC G[GA]^CG[CT]C
AhdI 4396 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2899 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4089 CCTAAGG CC^T[ACGT]AGG
AspEI 4396 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 3471 GCTGAGG CCTCAGC(-5/-2)
BcgI 1911 GCAAAGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3456 TGATCA T^GATCA
BglI 650 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3912 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3912 GCTAAGC GC^T[ACGT]AGC
BsaBI 1537 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2602 GGCGTC G[GA]^CG[CT]C
BsaI 4446 GAGACC GGTCTC(1/5)
BsaWI 4319 TCCGGA [AT]^CCGG[AT]
BseAI 4319 TCCGGA T^CCGGA
BsgI 1613 GTGCAG GTGCAG(16/14)
BspEI 4319 TCCGGA T^CCGGA
BsrFI 1825 GCCGGC [GA]^CCGG[CT]
BssSI 971 CACGAG CACGAG(-5/-1)
Bsu36I 4089 CCTAAGG CC^T[ACGT]AGG
CelII 3912 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1825 GCCGGC [GA]^CCGG[CT]
Eam1105I 4396 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4089 CCTAAGG CC^T[ACGT]AGG
EcoRI 3477 GAATTC G^AATTC
EcoRV 1558 GATATC GAT^ATC
EcoT22I 297 ATGCAT ATGCA^T
FbaI 3456 TGATCA T^GATCA
Hin1I 2602 GGCGTC G[GA]^CG[CT]C
MamI 1537 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3386 CAATTG C^AATTG
MroI 4319 TCCGGA T^CCGGA
MunI 3386 CAATTG C^AATTG
NaeI 1825 GCCGGC GCC^GGC
NgoMIV 1825 GCCGGC G^CCGGC
NsiI 297 ATGCAT ATGCA^T
SacI 776 GAGCTC GAGCT^C
SapI 1033 GCTCTTC GCTCTTC(1/4)
SmaI 2160 CCCGGG CCC^GGG
SmlI 2142 CTTGAG C^T[CT][GA]AG
SphI 1184 GCATGC GCATG^C
SspI 3548 AATATT AAT^ATT
StuI 657 AGGCCT AGG^CCT
XcmI 4382 CCATGAGTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2160 CCCGGG C^CCGGG
XmaI 2160 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1164 GGTACC G^GTACC
2963 GGTACC
AseI 1126 ATTAAT AT^TAAT
1572 ATTAAT
AsnI 1126 ATTAAT AT^TAAT
1572 ATTAAT
Asp700I 495 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1640 GAAGTAGTTC
Asp718I 1164 GGTACC G^GTACC
2963 GGTACC
BalI 326 TGGCCA TGG^CCA
2676 TGGCCA
BglII 1691 AGATCT A^GATCT
4472 AGATCT
BstEII 1472 GGTCACC G^GT[ACGT]ACC
3391 GGTGACC
BstPI 1472 GGTCACC G^GT[ACGT]ACC
3391 GGTGACC
BstXI 687 CCAGATATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4002 CCATTACAATGG
DraI 1683 TTTAAA TTT^AAA
1857 TTTAAA
DraIII 3164 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4427 CACAGGGTG
EaeI 326 TGGCCA [CT]^GGCC[GA]
2676 TGGCCA
EarI 1034 CTCTTC CTCTTC(1/4)
4110 CTCTTC
EcoNI 2257 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2830 CCTTCAGCAGG
EcoO65I 1472 GGTCACC G^GT[ACGT]ACC
3391 GGTGACC
FauI 2369 GCGGG CCCGC(4/6)
2551 GCGGG
KpnI 1164 GGTACC GGTAC^C
2963 GGTACC
Ksp632I 1034 CTCTTC CTCTTC(1/4)
4110 CTCTTC
MluNI 326 TGGCCA TGG^CCA
2676 TGGCCA
MscI 326 TGGCCA TGG^CCA
2676 TGGCCA
MspA1I 2206 CAGCTG C[AC]G^C[GT]G
2621 CAGCTG
NheI 1761 GCTAGC G^CTAGC
2365 GCTAGC
PflMI 637 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3193 CCATCTCTTGG
PpuMI 1431 AGGACCC [GA]G^G[AT]CC[CT]
1492 GGGTCCC
PshBI 1126 ATTAAT AT^TAAT
1572 ATTAAT
PvuII 2206 CAGCTG CAG^CTG
2621 CAGCTG
SexAI 1239 ACCTGGT A^CC[AT]GGT
3932 ACCTGGT
Van91I 637 CCATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3193 CCATCTCTTGG
XbaI 3507 TCTAGA T^CTAGA
3777 TCTAGA
XmnI 495 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1640 GAAGTAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI ApaLI ApoI AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII