(Circular) (Six-base) MAPSORT of: pFN21AA1461.seq Check: 7140 from: 1 to: 9187 pFN21AA1461 9187 bp With 182 enzymes: SgfI * February 26, 2010 09:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6997 278 Size: 53 83 186 936 5461 2468 Fragments arranged by size: 5461 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2595 6530 1937 Size: 658 3935 4594 Fragments arranged by size: 4594 3935 658 Acc65I G'GTAC_C Cuts at: 3169 4968 6508 3169 Size: 1799 1540 5848 Fragments arranged by size: 5848 1799 1540 AclI AA'CG_TT Cuts at: 1563 7314 7687 1563 Size: 5751 373 3063 Fragments arranged by size: 5751 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2817 3026 3867 4235 4536 4821 6045 7250 Size: 1185 209 841 368 301 285 1224 1205 Cuts at: 7250 8634 8902 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1224 1205 1185 841 368 301 285 268 209 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8990 AflIII A'CryG_T Cuts at: 1203 8086 9063 1203 Size: 6883 977 1327 Fragments arranged by size: 6883 1327 977 AgeI A'CCGG_T Cuts at: 8018 8018 Size: 9187 AhdI GACnn_n'nnGTC Cuts at: 6406 7916 6406 Size: 1510 7677 AleI CACnn'nnGTG Cuts at: 1166 4469 1166 Size: 3303 5884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3732 5091 5318 1184 Size: 2548 1359 227 5053 Fragments arranged by size: 5053 2548 1359 227 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6092 6124 6092 Size: 32 9155 AlwNI CAG_nnn'CTG Cuts at: 4909 6930 8502 4909 Size: 2021 1572 5594 Fragments arranged by size: 5594 2021 1572 ApaLI G'TGCA_C Cuts at: 3332 4280 5865 7244 8400 3332 Size: 948 1585 1379 1156 4119 Fragments arranged by size: 4119 1585 1379 1156 948 ApoI r'AATT_y Cuts at: 1493 2476 3415 4417 4449 5482 5645 5778 5791 Size: 983 939 1002 32 1033 163 133 13 Cuts at: 5791 5983 6496 6722 6874 8983 1493 Size: 192 513 226 152 2109 1697 Fragments arranged by size: 2109 1697 1033 1002 983 939 513 226 192 163 152 133 32 13 AseI AT'TA_AT Cuts at: 160 1032 3132 3578 7741 160 Size: 872 2100 446 4163 1606 Fragments arranged by size: 4163 2100 1606 872 446 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9187 AvaI C'yCGr_G Cuts at: 1870 1943 2424 4165 6254 6275 6512 1870 Size: 73 481 1741 2089 21 237 4545 Fragments arranged by size: 4545 2089 1741 481 237 73 21 BamHI G'GATC_C Cuts at: 6517 6884 6517 Size: 367 8820 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3169 4559 4968 6508 7963 Size: 325 217 627 1382 1390 409 1540 1455 Cuts at: 7963 618 Size: 1842 Fragments arranged by size: 1842 1540 1455 1390 1382 627 409 325 217 BanII G_rGCy'C Cuts at: 1391 2785 5118 5497 6276 6506 1391 Size: 1394 2333 379 779 230 4072 Fragments arranged by size: 4072 2333 1394 779 379 230 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2042 6228 6293 928 Size: 971 143 4186 65 3822 Fragments arranged by size: 4186 3822 971 143 65 BbvCI CC'TCA_GC Cuts at: 5477 5477 Size: 9187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3905 3939 7378 7412 1903 Size: 34 1968 34 3439 34 3678 Fragments arranged by size: 3678 3439 1968 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7079 8289 7079 Size: 1210 7977 BclI T'GATC_A Cuts at: 1548 5461 1548 Size: 3913 5274 BfrBI ATG'CAT Cuts at: 2304 2304 Size: 9187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2661 7798 136 Size: 107 122 71 631 605 989 5137 1525 Fragments arranged by size: 5137 1525 989 631 605 122 107 71 BglII A'GATC_T Cuts at: 3696 6477 9182 3696 Size: 2781 2705 3701 Fragments arranged by size: 3701 2781 2705 BlpI GC'TnA_GC Cuts at: 5918 6604 5918 Size: 686 8501 Bme1580I G_kGCm'C Cuts at: 1790 3336 4284 4562 5869 7248 8404 1790 Size: 1546 948 278 1307 1379 1156 2573 Fragments arranged by size: 2573 1546 1379 1307 1156 948 278 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9187 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2115 4224 6918 Size: 416 53 83 399 24 698 2109 2694 Cuts at: 6918 7876 442 Size: 958 1753 Fragments arranged by size: 2694 2109 1753 958 698 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3770 4374 1056 Size: 2714 604 5869 Fragments arranged by size: 5869 2714 604 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2259 2394 3300 3840 4452 4814 4946 4964 Size: 1020 135 906 540 612 362 132 18 Cuts at: 4964 5398 6466 7847 1239 Size: 434 1068 1381 2579 Fragments arranged by size: 2579 1381 1068 1020 906 612 540 434 362 135 132 18 Bpu10I CC'TnA_GC Cuts at: 4067 5477 4067 Size: 1410 7777 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4168 7312 8177 8475 4168 Size: 3144 865 298 4880 Fragments arranged by size: 4880 3144 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6445 7850 882 Size: 617 4946 1405 2219 Fragments arranged by size: 4946 2219 1405 617 BsaAI yAC'GTr Cuts at: 493 1204 6978 493 Size: 711 5774 2702 Fragments arranged by size: 5774 2702 711 BsaBI GATnn'nnATC Cuts at: 3546 9181 3546 Size: 5635 3552 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4608 6994 7376 8011 Size: 53 83 186 936 3075 2386 382 635 Cuts at: 8011 275 Size: 1451 Fragments arranged by size: 3075 2386 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6324 7620 8018 8292 8439 8850 6324 Size: 1296 398 274 147 411 6661 Fragments arranged by size: 6661 1296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2232 2262 2568 2598 4785 Size: 30 165 30 829 30 306 30 2187 Cuts at: 4785 4815 6092 6122 1178 Size: 30 1277 30 4243 Fragments arranged by size: 4243 2187 1277 829 306 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2406 2808 3012 4748 5963 6484 1186 Size: 1220 402 204 1736 1215 521 3889 Fragments arranged by size: 3889 1736 1220 1215 521 402 204 BseYI C'CCAG_C Cuts at: 2669 2674 3676 4751 5014 8390 8886 2669 Size: 5 1002 1075 263 3376 496 2970 Fragments arranged by size: 3376 2970 1075 1002 496 263 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3639 1163 Size: 573 1903 6711 Fragments arranged by size: 6711 1903 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6637 7398 8426 1540 Size: 275 186 4636 761 1028 2301 Fragments arranged by size: 4636 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2379 2785 3040 3336 4284 4692 5869 7248 7333 Size: 406 255 296 948 408 1177 1379 85 Cuts at: 7333 8404 2379 Size: 1071 3162 Fragments arranged by size: 3162 1379 1177 1071 948 408 406 296 255 85 BsmI GAATG_Cn' Cuts at: 3081 3286 3687 5307 6698 6791 3081 Size: 205 401 1620 1391 93 5477 Fragments arranged by size: 5477 1620 1391 401 205 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2379 2785 3040 3336 4284 4562 4692 Size: 399 589 406 255 296 948 278 130 Cuts at: 4692 5118 5497 5869 6276 6506 7248 7333 8404 Size: 426 379 372 407 230 742 85 1071 Cuts at: 8404 1391 Size: 2174 Fragments arranged by size: 2174 1071 948 742 589 426 407 406 399 379 372 296 278 255 230 130 85 BspEI T'CCGG_A Cuts at: 6324 8850 6324 Size: 2526 6661 BspHI T'CATG_A Cuts at: 2461 3420 5464 5686 6379 7076 8806 2461 Size: 959 2044 222 693 697 1730 2842 Fragments arranged by size: 2842 2044 1730 959 697 693 222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2140 2172 4337 5296 6542 844 Size: 1296 32 2165 959 1246 3489 Fragments arranged by size: 3489 2165 1296 1246 959 32 BsrBI CCG'CTC Cuts at: 1394 1823 7074 1394 Size: 429 5251 3507 Fragments arranged by size: 5251 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2880 2988 3227 3968 7682 7856 65 Size: 1183 1632 108 239 741 3714 174 1396 Fragments arranged by size: 3714 1632 1396 1183 741 239 174 108 BsrFI r'CCGG_y Cuts at: 1700 1810 3830 7831 8018 1700 Size: 110 2020 4001 187 2869 Fragments arranged by size: 4001 2869 2020 187 110 BsrGI T'GTAC_A Cuts at: 96 2551 2857 3271 6418 96 Size: 2455 306 414 3147 2865 Fragments arranged by size: 3147 2865 2455 414 306 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9187 BssSI C'ACGA_G Cuts at: 2976 7247 8259 2976 Size: 4271 1012 3904 Fragments arranged by size: 4271 3904 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9187 BstBI TT'CG_AA Cuts at: 7991 7991 Size: 9187 BstEII G'GTnAC_C Cuts at: 1184 3477 5396 1184 Size: 2293 1919 4975 Fragments arranged by size: 4975 2293 1919 BstXI CCAn_nnnn'nTGG Cuts at: 2699 6014 2699 Size: 3315 5872 BstYI r'GATC_y Cuts at: 1972 3070 3696 4225 6477 6517 6884 7269 7286 Size: 1098 626 529 2252 40 367 385 17 Cuts at: 7286 8738 8824 8836 9182 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2252 1977 1452 1098 626 529 385 367 346 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 6095 6095 Size: 9187 BtgI C'CryG_G Cuts at: 513 1065 2535 2647 2656 5031 5518 513 Size: 552 1470 112 9 2375 487 4182 Fragments arranged by size: 4182 2375 1470 552 487 112 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3354 487 Size: 683 56 2128 6320 Fragments arranged by size: 6320 2128 683 56 BtsI GCAGTG_nn' Cuts at: 2334 2626 3622 3841 4282 4620 4637 5319 5382 Size: 292 996 219 441 338 17 682 63 Cuts at: 5382 5711 5922 6703 7497 7517 2334 Size: 329 211 781 794 20 4004 Fragments arranged by size: 4004 996 794 781 682 441 338 329 292 219 211 63 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9187 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4725 4760 529 Size: 35 4161 35 4956 Fragments arranged by size: 4956 4161 35 35 DraI TTT'AAA Cuts at: 3690 3864 6491 3690 Size: 174 2627 6386 Fragments arranged by size: 6386 2627 174 DraIII CAC_nnn'GTG Cuts at: 5174 6437 5174 Size: 1263 7924 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8194 809 Size: 507 6878 1802 Fragments arranged by size: 6878 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2331 4681 6634 Size: 54 1425 50 275 18 501 2350 1953 Cuts at: 6634 7523 9152 8 Size: 889 1629 43 Fragments arranged by size: 2350 1953 1629 1425 889 501 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6634 1537 Size: 275 4822 4090 Fragments arranged by size: 4822 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3045 6121 7117 1353 Size: 1692 3076 996 3423 Fragments arranged by size: 3423 3076 1692 996 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7774 8148 8294 387 Size: 1064 138 6185 374 146 1280 Fragments arranged by size: 6185 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2783 2783 Size: 9187 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2259 2394 2817 3026 3300 3840 3867 Size: 393 627 135 423 209 274 540 27 Cuts at: 3867 4235 4452 4536 4814 4821 4946 4964 5398 Size: 368 217 84 278 7 125 18 434 Cuts at: 5398 6045 6466 7250 7847 8634 8902 1239 Size: 647 421 784 597 787 268 1524 Fragments arranged by size: 1524 787 784 647 627 597 540 434 423 421 393 368 278 274 268 217 209 135 125 84 27 18 7 EcoNI CCTnn'n_nnAGG Cuts at: 4266 4839 4266 Size: 573 8614 EcoO109I rG'GnC_Cy Cuts at: 3437 3498 4176 3437 Size: 61 678 8448 Fragments arranged by size: 8448 678 61 EcoRI G'AATT_C Cuts at: 5482 6496 6874 5482 Size: 1014 378 7795 Fragments arranged by size: 7795 1014 378 EcoRV GAT'ATC Cuts at: 3565 3565 Size: 9187 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2066 2209 3092 2066 Size: 143 883 8161 Fragments arranged by size: 8161 883 143 FspI TGC'GCA Cuts at: 7693 7693 Size: 9187 HaeII r_GCGC'y Cuts at: 1678 8334 1678 Size: 6656 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4378 4410 4844 4870 4876 Size: 32 135 32 2806 32 434 26 6 Cuts at: 4876 4902 4918 4950 5939 5971 6098 6130 6392 Size: 26 16 32 989 32 127 32 262 Cuts at: 6392 6424 7828 7860 7902 7934 1373 Size: 32 1404 32 42 32 2626 Fragments arranged by size: 2806 2626 1404 989 434 262 135 127 42 32 32 32 32 32 32 32 32 32 26 26 16 6 HincII GTy'rAC Cuts at: 669 1657 1938 3489 5210 5329 6245 6531 6777 Size: 988 281 1551 1721 119 916 286 246 Cuts at: 6777 669 Size: 3079 Fragments arranged by size: 3079 1721 1551 988 916 286 281 246 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 9187 HpaI GTT'AAC Cuts at: 6777 6777 Size: 9187 KpnI G_GTAC'C Cuts at: 3173 4972 6512 3173 Size: 1799 1540 5848 Fragments arranged by size: 5848 1799 1540 MfeI C'AATT_G Cuts at: 5391 6786 5391 Size: 1395 7792 MluI A'CGCG_T Cuts at: 9063 9063 Size: 9187 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3161 4734 8301 8485 1305 Size: 1856 1573 3567 184 2007 Fragments arranged by size: 3567 2007 1856 1573 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 4683 10 Size: 54 1425 343 501 2350 4514 Fragments arranged by size: 4514 2350 1425 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2185 3153 4469 5874 6012 7145 7504 Size: 648 1019 968 1316 1405 138 1133 359 Cuts at: 7504 7663 518 Size: 159 2042 Fragments arranged by size: 2042 1405 1316 1133 1019 968 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 4213 4628 6603 7280 8428 8673 1606 Size: 2607 415 1975 677 1148 245 2120 Fragments arranged by size: 2607 2120 1975 1148 677 415 245 NaeI GCC'GGC Cuts at: 3832 3832 Size: 9187 NcoI C'CATG_G Cuts at: 513 1065 2535 2656 5031 5518 513 Size: 552 1470 121 2375 487 4182 Fragments arranged by size: 4182 2375 1470 552 487 121 NdeI CA'TA_TG Cuts at: 387 387 Size: 9187 NgoMIV G'CCGG_C Cuts at: 3830 3830 Size: 9187 NheI G'CTAG_C Cuts at: 1052 3766 4370 1052 Size: 2714 604 5869 Fragments arranged by size: 5869 2714 604 NotI GC'GGCC_GC Cuts at: 6634 6634 Size: 9187 NsiI A_TGCA'T Cuts at: 2306 2306 Size: 9187 NspI r_CATG'y Cuts at: 1132 1229 2184 3193 3895 6545 8090 1132 Size: 97 955 1009 702 2650 1545 2229 Fragments arranged by size: 2650 2229 1545 1009 955 702 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9187 PciI A'CATG_T Cuts at: 8086 8086 Size: 9187 PflMI CCAn_nnn'nTGG Cuts at: 1108 2648 5204 1108 Size: 1540 2556 5091 Fragments arranged by size: 5091 2556 1540 PfoI T'CCnGG_A Cuts at: 2236 2386 2236 Size: 150 9037 PmeI GTTT'AAAC Cuts at: 6491 6491 Size: 9187 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6092 6124 7256 7288 8769 8801 6092 Size: 32 1132 32 1481 32 6478 Fragments arranged by size: 6478 1481 1132 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3437 3498 3437 Size: 61 9126 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9187 PsiI TTA'TAA Cuts at: 6757 6757 Size: 9187 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3264 3296 3264 Size: 32 9155 PstI C_TGCA'G Cuts at: 830 4042 4627 4644 5326 6539 830 Size: 3212 585 17 682 1213 3478 Fragments arranged by size: 3478 3212 1213 682 585 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9187 PvuII CAG'CTG Cuts at: 4213 4628 4213 Size: 415 8772 SacI G_AGCT'C Cuts at: 2785 2785 Size: 9187 SalI G'TCGA_C Cuts at: 1936 6529 1936 Size: 4593 4594 SanDI GG'GwC_CC Cuts at: 3498 3498 Size: 9187 SapI GCTCTTCn'nnn_ Cuts at: 3045 3045 Size: 9187 SbfI CC_TGCA'GG Cuts at: 6539 6539 Size: 9187 ScaI AGT'ACT Cuts at: 7435 9147 7435 Size: 1712 7475 SexAI A'CCwGG_T Cuts at: 3244 5937 3244 Size: 2693 6494 SfcI C'TryA_G Cuts at: 826 1045 3432 4038 4118 4623 4640 4901 5322 Size: 219 2387 606 80 505 17 261 421 Cuts at: 5322 6313 6535 7670 8351 8542 826 Size: 991 222 1135 681 191 1471 Fragments arranged by size: 2387 1471 1135 991 681 606 505 421 261 222 219 191 80 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9187 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9187 SmaI CCC'GGG Cuts at: 1872 4167 6514 1872 Size: 2295 2347 4545 Fragments arranged by size: 4545 2347 2295 SmlI C'TyrA_G Cuts at: 820 1017 1943 4147 7291 8192 8454 820 Size: 197 926 2204 3144 901 262 1553 Fragments arranged by size: 3144 2204 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6978 493 Size: 6485 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9187 SphI G_CATG'C Cuts at: 1132 3193 6545 1132 Size: 2061 3352 3774 Fragments arranged by size: 3774 3352 2061 SspI AAT'ATT Cuts at: 5 52 5555 7111 5 Size: 47 5503 1556 2081 Fragments arranged by size: 5503 2081 1556 47 StuI AGG'CCT Cuts at: 1517 2664 1517 Size: 1147 8040 StyI C'CwwG_G Cuts at: 513 1065 2217 2535 2656 2720 3004 3482 3503 Size: 552 1152 318 121 64 284 478 21 Cuts at: 3503 4990 5031 5518 6143 6626 513 Size: 1487 41 487 625 483 3074 Fragments arranged by size: 3074 1487 1152 625 552 487 483 478 318 284 121 64 41 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3487 5225 5754 6033 7214 7231 7384 Size: 1255 1907 1738 529 279 1181 17 153 Cuts at: 7384 7569 8014 325 Size: 185 445 1498 Fragments arranged by size: 1907 1738 1498 1255 1181 529 445 279 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2551 2857 3271 Size: 274 80 33 51 1443 574 306 414 Cuts at: 3271 3555 3598 5333 6418 7433 9145 96 Size: 284 43 1735 1085 1015 1712 138 Fragments arranged by size: 1735 1712 1443 1085 1015 574 414 306 284 274 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2232 2264 5110 5134 5142 5166 9147 9179 2232 Size: 32 2846 24 8 24 3981 32 2240 Fragments arranged by size: 3981 2846 2240 32 32 24 24 8 Tth111I GACn'n_nGTC Cuts at: 1316 5895 5939 6167 1316 Size: 4579 44 228 4336 Fragments arranged by size: 4579 4336 228 44 XbaI T'CTAG_A Cuts at: 5512 5782 6523 5512 Size: 270 741 8176 Fragments arranged by size: 8176 741 270 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6394 6394 Size: 9187 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9187 XmaI C'CCGG_G Cuts at: 1870 4165 6512 1870 Size: 2295 2347 4545 Fragments arranged by size: 4545 2347 2295 XmnI GAAnn'nnTTC Cuts at: 2504 3649 7316 2504 Size: 1145 3667 4375 Fragments arranged by size: 4375 3667 1145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6995 276 Size: 53 83 186 936 5461 2468 Fragments arranged by size: 5461 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI RsrII SacII SfiI SfoI SrfI SwaI