(Circular) (Six-base) MAPSORT of: pFN21AA0561.seq Check: 6143 from: 1 to: 8632 pFN21AA0561 8632 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2898 3610 5647 2898 Size: 712 2037 5883 Fragments arranged by size: 5883 2037 712 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4932 5061 6442 278 Size: 53 83 186 936 3396 129 1381 2468 Fragments arranged by size: 3396 2468 1381 936 186 129 83 53 AccI GT'mk_AC Cuts at: 1937 3147 4125 4911 4932 5975 1937 Size: 1210 978 786 21 1043 4594 Fragments arranged by size: 4594 1210 1043 978 786 21 Acc65I G'GTAC_C Cuts at: 5953 5953 Size: 8632 AclI AA'CG_TT Cuts at: 1563 4024 6759 7132 1563 Size: 2461 2735 373 3063 Fragments arranged by size: 3063 2735 2461 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3392 4168 4873 5096 6695 8079 8347 1632 Size: 1760 776 705 223 1599 1384 268 1917 Fragments arranged by size: 1917 1760 1599 1384 776 705 268 223 AfeI AGC'GCT Cuts at: 3179 3179 Size: 8632 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8435 AflIII A'CryG_T Cuts at: 1203 2526 4013 7531 8508 1203 Size: 1323 1487 3518 977 1327 Fragments arranged by size: 3518 1487 1327 1323 977 AgeI A'CCGG_T Cuts at: 4110 7463 4110 Size: 3353 5279 AhdI GACnn_n'nnGTC Cuts at: 7361 7361 Size: 8632 AleI CACnn'nnGTG Cuts at: 1166 4472 5864 1166 Size: 3306 1392 3934 Fragments arranged by size: 3934 3306 1392 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3198 1184 Size: 2014 6618 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4778 4810 4778 Size: 32 8600 AlwNI CAG_nnn'CTG Cuts at: 3041 4409 4625 4697 5384 5459 6375 7947 3041 Size: 1368 216 72 687 75 916 1572 3726 Fragments arranged by size: 3726 1572 1368 916 687 216 75 72 ApaI G_GGCC'C Cuts at: 3386 4610 5141 5919 3386 Size: 1224 531 778 6099 Fragments arranged by size: 6099 1224 778 531 ApaLI G'TGCA_C Cuts at: 3464 4940 5048 6689 7845 3464 Size: 1476 108 1641 1156 4251 Fragments arranged by size: 4251 1641 1476 1156 108 ApoI r'AATT_y Cuts at: 1493 2194 2218 2879 5941 6167 6319 8428 1493 Size: 701 24 661 3062 226 152 2109 1697 Fragments arranged by size: 3062 2109 1697 701 661 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 7186 160 Size: 872 6154 1606 Fragments arranged by size: 6154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8632 AvaI C'yCGr_G Cuts at: 1870 1943 2331 2476 2670 3600 3650 3758 4310 Size: 73 388 145 194 930 50 108 552 Cuts at: 4310 4489 5428 5621 5912 5957 1870 Size: 179 939 193 291 45 4545 Fragments arranged by size: 4545 939 930 552 388 291 194 193 179 145 108 73 50 45 BamHI G'GATC_C Cuts at: 4280 5962 6329 4280 Size: 1682 367 6583 Fragments arranged by size: 6583 1682 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 2606 3001 3159 3315 Size: 325 217 627 241 578 395 158 156 Cuts at: 3315 3893 4550 5625 5953 7408 618 Size: 578 657 1075 328 1455 1842 Fragments arranged by size: 1842 1455 1075 657 627 578 578 395 328 325 241 217 158 156 BanII G_rGCy'C Cuts at: 1391 2040 3386 3869 4043 4610 4760 5141 5435 Size: 649 1346 483 174 567 150 381 294 Cuts at: 5435 5748 5919 5951 1391 Size: 313 171 32 4072 Fragments arranged by size: 4072 1346 649 567 483 381 313 294 174 171 150 32 BbeI G_GCGC'C Cuts at: 2032 2610 3319 4554 2032 Size: 578 709 1235 6110 Fragments arranged by size: 6110 1235 709 578 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4197 4423 928 Size: 971 2298 226 5137 Fragments arranged by size: 5137 2298 971 226 BbvCI CC'TCA_GC Cuts at: 2904 4259 2904 Size: 1355 7277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2005 2039 4493 4527 4528 4562 6823 Size: 34 68 34 2454 34 1 34 2261 Cuts at: 6823 6857 1903 Size: 34 3678 Fragments arranged by size: 3678 2454 2261 68 34 34 34 34 34 1 BciVI GTATCCnnnnn_n' Cuts at: 6524 7734 6524 Size: 1210 7422 BclI T'GATC_A Cuts at: 1548 2823 1548 Size: 1275 7357 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2237 2615 2845 Size: 107 122 71 631 605 565 378 230 Cuts at: 2845 3140 4038 4413 4605 4985 5325 7243 136 Size: 295 898 375 192 380 340 1918 1525 Fragments arranged by size: 1918 1525 898 631 605 565 380 378 375 340 295 230 192 122 107 71 BglII A'GATC_T Cuts at: 5238 8627 5238 Size: 3389 5243 BlpI GC'TnA_GC Cuts at: 2051 6049 2051 Size: 3998 4634 Bme1580I G_kGCm'C Cuts at: 1790 3386 3468 3887 3975 4610 4686 4944 5052 Size: 1596 82 419 88 635 76 258 108 Cuts at: 5052 5141 5628 5690 5919 6693 7849 1790 Size: 89 487 62 229 774 1156 2573 Fragments arranged by size: 2573 1596 1156 774 635 487 419 258 229 108 89 88 82 76 62 BmgBI CAC'GTC Cuts at: 1318 2072 4948 1318 Size: 754 2876 5002 Fragments arranged by size: 5002 2876 754 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3946 4084 4466 Size: 416 53 83 399 24 2529 138 382 Cuts at: 4466 5859 6363 7321 442 Size: 1393 504 958 1753 Fragments arranged by size: 2529 1753 1393 958 504 416 399 382 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8632 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5140 5140 Size: 8632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2024 2762 2873 2882 2915 3011 3458 4630 Size: 785 738 111 9 33 96 447 1172 Cuts at: 4630 4975 5135 5419 7292 1239 Size: 345 160 284 1873 2579 Fragments arranged by size: 2579 1873 1172 785 738 447 345 284 160 111 96 33 9 Bpu10I CC'TnA_GC Cuts at: 2904 4259 2904 Size: 1355 7277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6757 7622 7920 6757 Size: 865 298 7469 Fragments arranged by size: 7469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3101 3302 4049 4608 4812 5198 7295 Size: 617 1602 201 747 559 204 386 2097 Cuts at: 7295 882 Size: 2219 Fragments arranged by size: 2219 2097 1602 747 617 559 386 204 201 BsaAI yAC'GTr Cuts at: 493 1204 2527 3343 6423 493 Size: 711 1323 816 3080 2702 Fragments arranged by size: 3080 2702 1323 816 711 BsaBI GATnn'nnATC Cuts at: 2828 8626 2828 Size: 5798 2834 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2029 2366 2607 2631 Size: 53 83 186 936 496 337 241 24 Cuts at: 2631 3316 3597 3632 3705 3831 4551 4929 5058 Size: 685 281 35 73 126 720 378 129 Cuts at: 5058 6439 6821 7456 275 Size: 1381 382 635 1451 Fragments arranged by size: 1451 1381 936 720 685 635 496 382 378 337 281 241 186 129 126 83 73 53 35 24 BsaWI w'CCGG_w Cuts at: 2811 4110 5285 5921 7065 7463 7737 7884 8295 Size: 1299 1175 636 1144 398 274 147 411 Cuts at: 8295 2811 Size: 3148 Fragments arranged by size: 3148 1299 1175 1144 636 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2433 2463 4171 4190 4201 Size: 30 165 30 1030 30 1708 19 11 Cuts at: 4201 4220 5330 5360 1178 Size: 19 1110 30 4450 Fragments arranged by size: 4450 1708 1110 1030 165 30 30 30 30 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2798 3008 4087 4214 4633 5203 5272 5855 Size: 1612 210 1079 127 419 570 69 583 Cuts at: 5855 1186 Size: 3963 Fragments arranged by size: 3963 1612 1079 583 570 419 210 127 69 BseYI C'CCAG_C Cuts at: 2300 2968 3034 3975 4305 4322 4618 5000 5038 Size: 668 66 941 330 17 296 382 38 Cuts at: 5038 5351 5377 5392 5417 5452 5477 5683 7835 Size: 313 26 15 25 35 25 206 2152 Cuts at: 7835 8331 2300 Size: 496 2601 Fragments arranged by size: 2601 2152 941 668 496 382 330 313 296 206 66 38 35 26 25 25 17 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2363 5246 5471 5750 5831 1163 Size: 573 627 2883 225 279 81 3964 Fragments arranged by size: 3964 2883 627 573 279 225 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2437 4588 6082 6843 7871 1540 Size: 275 186 436 2151 1494 761 1028 2301 Fragments arranged by size: 2301 2151 1494 1028 761 436 275 186 BsiHKAI G_wGCw'C Cuts at: 3468 4944 5052 5435 5748 6693 6778 7849 3468 Size: 1476 108 383 313 945 85 1071 4251 Fragments arranged by size: 4251 1476 1071 945 383 313 108 85 BsmI GAATG_Cn' Cuts at: 6143 6236 6143 Size: 93 8539 BsmBI CGTCTCn'nnnn_ Cuts at: 2271 2345 3416 5922 2271 Size: 74 1071 2506 4981 Fragments arranged by size: 4981 2506 1071 74 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 3386 3468 3869 3887 3975 4043 Size: 399 250 1346 82 401 18 88 68 Cuts at: 4043 4610 4686 4760 4944 5052 5141 5435 5628 Size: 567 76 74 184 108 89 294 193 Cuts at: 5628 5690 5748 5919 5951 6693 6778 7849 1391 Size: 62 58 171 32 742 85 1071 2174 Fragments arranged by size: 2174 1346 1071 742 567 401 399 294 250 193 184 171 108 89 88 85 82 76 74 68 62 58 32 18 BspEI T'CCGG_A Cuts at: 2811 5285 8295 2811 Size: 2474 3010 3148 Fragments arranged by size: 3148 3010 2474 BspHI T'CATG_A Cuts at: 2514 3552 4752 6521 8251 2514 Size: 1038 1200 1769 1730 2895 Fragments arranged by size: 2895 1769 1730 1200 1038 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2898 3226 3453 3610 5647 5987 844 Size: 2054 328 227 157 2037 340 3489 Fragments arranged by size: 3489 2054 2037 340 328 227 157 BsrBI CCG'CTC Cuts at: 1394 1823 2460 2746 4315 5082 5604 5698 6519 Size: 429 637 286 1569 767 522 94 821 Cuts at: 6519 1394 Size: 3507 Fragments arranged by size: 3507 1569 821 767 637 522 429 286 94 BsrDI GCAATG_nn' Cuts at: 65 1248 7127 7301 65 Size: 1183 5879 174 1396 Fragments arranged by size: 5879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2436 4110 4236 4583 7276 7463 1700 Size: 110 626 1674 126 347 2693 187 2869 Fragments arranged by size: 2869 2693 1674 626 347 187 126 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8632 BssSI C'ACGA_G Cuts at: 5727 5747 6692 7704 5727 Size: 20 945 1012 6655 Fragments arranged by size: 6655 1012 945 20 BstAPI GCAn_nnn'nTGC Cuts at: 794 3297 794 Size: 2503 6129 BstBI TT'CG_AA Cuts at: 7436 7436 Size: 8632 BstEII G'GTnAC_C Cuts at: 1184 2439 1184 Size: 1255 7377 BstXI CCAn_nnnn'nTGG Cuts at: 3674 3690 4351 4871 5113 5134 3674 Size: 16 661 520 242 21 7172 Fragments arranged by size: 7172 661 520 242 21 16 BstYI r'GATC_y Cuts at: 1972 3228 4083 4280 5238 5962 6329 6714 6731 Size: 1256 855 197 958 724 367 385 17 Cuts at: 6731 8183 8269 8281 8627 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1256 958 855 724 385 367 346 197 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2874 2874 Size: 8632 BtgI C'CryG_G Cuts at: 513 1065 2004 2032 3393 3699 5651 5880 513 Size: 552 939 28 1361 306 1952 229 3265 Fragments arranged by size: 3265 1952 1361 939 552 306 229 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3801 487 Size: 683 56 2575 5318 Fragments arranged by size: 5318 2575 683 56 BtsI GCAGTG_nn' Cuts at: 4663 4675 5403 6148 6942 6962 4663 Size: 12 728 745 794 20 6333 Fragments arranged by size: 6333 794 745 728 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8597 DraI TTT'AAA Cuts at: 5936 5936 Size: 8632 DraIII CAC_nnn'GTG Cuts at: 3904 3904 Size: 8632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7639 809 Size: 507 6323 1802 Fragments arranged by size: 6323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2434 2682 2837 Size: 54 1425 50 275 18 604 248 155 Cuts at: 2837 2976 3070 3508 3561 3800 4146 4231 4464 Size: 139 94 438 53 239 346 85 233 Cuts at: 4464 4585 5157 5464 5530 6079 6968 8597 8 Size: 121 572 307 66 549 889 1629 43 Fragments arranged by size: 1629 1425 889 604 572 549 438 346 307 275 248 239 233 155 139 121 94 85 66 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2434 4585 6079 1537 Size: 275 622 2151 1494 4090 Fragments arranged by size: 4090 2151 1494 622 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3773 4810 5072 5920 6562 1353 Size: 2420 1037 262 848 642 3423 Fragments arranged by size: 3423 2420 1037 848 642 262 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3653 5233 7219 7593 7739 387 Size: 1064 138 2064 1580 1986 374 146 1280 Fragments arranged by size: 2064 1986 1580 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5433 5746 5433 Size: 313 8319 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2024 2762 2873 2882 2915 3011 3392 Size: 393 392 738 111 9 33 96 381 Cuts at: 3392 3458 4168 4630 4873 4975 5096 5135 5419 Size: 66 710 462 243 102 121 39 284 Cuts at: 5419 6695 7292 8079 8347 1239 Size: 1276 597 787 268 1524 Fragments arranged by size: 1524 1276 787 738 710 597 462 393 392 381 284 268 243 121 111 102 96 66 39 33 9 EcoNI CCTnn'n_nnAGG Cuts at: 2894 2978 3575 2894 Size: 84 597 7951 Fragments arranged by size: 7951 597 84 EcoO109I rG'GnC_Cy Cuts at: 2493 2983 3817 3838 4263 4319 4406 4415 4606 Size: 490 834 21 425 56 87 9 191 Cuts at: 4606 5005 5138 2493 Size: 399 133 5987 Fragments arranged by size: 5987 834 490 425 399 191 133 87 56 21 9 EcoRI G'AATT_C Cuts at: 5941 6319 5941 Size: 378 8254 EcoRV GAT'ATC Cuts at: 5094 5094 Size: 8632 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4390 4903 4390 Size: 513 8119 FspI TGC'GCA Cuts at: 7138 7138 Size: 8632 HaeII r_GCGC'y Cuts at: 1678 2032 2610 2621 3181 3319 3437 4554 5782 Size: 354 578 11 560 138 118 1117 1228 Cuts at: 5782 7779 1678 Size: 1997 2531 Fragments arranged by size: 2531 1997 1228 1117 578 560 354 138 118 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2244 2276 3594 3626 4055 Size: 32 135 32 672 32 1318 32 429 Cuts at: 4055 4087 5179 5197 5211 5229 7273 7305 7347 Size: 32 1092 18 14 18 2044 32 42 Cuts at: 7347 7379 1373 Size: 32 2626 Fragments arranged by size: 2626 2044 1318 1092 672 429 135 42 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 5976 6222 669 Size: 988 281 4038 246 3079 Fragments arranged by size: 4038 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 5504 748 Size: 4756 3876 HpaI GTT'AAC Cuts at: 6222 6222 Size: 8632 KasI G'GCGC_C Cuts at: 2028 2606 3315 4550 2028 Size: 578 709 1235 6110 Fragments arranged by size: 6110 1235 709 578 KpnI G_GTAC'C Cuts at: 5957 5957 Size: 8632 MfeI C'AATT_G Cuts at: 6231 6231 Size: 8632 MluI A'CGCG_T Cuts at: 8508 8508 Size: 8632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2476 3976 7746 7930 1305 Size: 1171 1500 3770 184 2007 Fragments arranged by size: 3770 2007 1500 1171 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2684 2978 3510 3563 3802 Size: 54 1425 343 852 294 532 53 239 Cuts at: 3802 5159 5466 10 Size: 1357 307 3176 Fragments arranged by size: 3176 1425 1357 852 532 343 307 294 239 54 53 MslI CAynn'nnrTG Cuts at: 518 1166 3453 3557 4472 5132 5864 6590 6949 Size: 648 2287 104 915 660 732 726 359 Cuts at: 6949 7108 518 Size: 159 2042 Fragments arranged by size: 2287 2042 915 732 726 660 648 359 159 104 MspA1I CmG'CkG Cuts at: 1606 2034 2085 2968 3388 3871 4463 4511 4650 Size: 428 51 883 420 483 592 48 139 Cuts at: 4650 4740 5456 5569 5653 5675 6048 6725 7873 Size: 90 716 113 84 22 373 677 1148 Cuts at: 7873 8118 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 883 716 677 592 483 428 420 373 245 139 113 90 84 51 48 22 NaeI GCC'GGC Cuts at: 4238 4585 4238 Size: 347 8285 NarI GG'CG_CC Cuts at: 2029 2607 3316 4551 2029 Size: 578 709 1235 6110 Fragments arranged by size: 6110 1235 709 578 NcoI C'CATG_G Cuts at: 513 1065 2004 3699 513 Size: 552 939 1695 5446 Fragments arranged by size: 5446 1695 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8632 NgoMIV G'CCGG_C Cuts at: 4236 4583 4236 Size: 347 8285 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8632 NotI GC'GGCC_GC Cuts at: 6079 6079 Size: 8632 NspI r_CATG'y Cuts at: 1132 1229 3295 5990 7535 1132 Size: 97 2066 2695 1545 2229 Fragments arranged by size: 2695 2229 2066 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8632 PciI A'CATG_T Cuts at: 7531 7531 Size: 8632 PflMI CCAn_nnn'nTGG Cuts at: 1108 2893 1108 Size: 1785 6847 PfoI T'CCnGG_A Cuts at: 4265 4265 Size: 8632 PmeI GTTT'AAAC Cuts at: 5936 5936 Size: 8632 PmlI CAC'GTG Cuts at: 2527 2527 Size: 8632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6701 6733 8214 8246 6701 Size: 32 1481 32 7087 Fragments arranged by size: 7087 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2493 2983 3838 4263 4319 5005 2493 Size: 490 855 425 56 686 6120 Fragments arranged by size: 6120 855 686 490 425 56 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8632 PsiI TTA'TAA Cuts at: 6202 6202 Size: 8632 PspOMI G'GGCC_C Cuts at: 3382 4606 5137 5915 3382 Size: 1224 531 778 6099 Fragments arranged by size: 6099 1224 778 531 PspXI vC'TCGA_Gb Cuts at: 4489 4489 Size: 8632 PstI C_TGCA'G Cuts at: 830 2580 2757 4414 5984 830 Size: 1750 177 1657 1570 3478 Fragments arranged by size: 3478 1750 1657 1570 177 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8632 PvuII CAG'CTG Cuts at: 2968 4511 5456 5675 2968 Size: 1543 945 219 5925 Fragments arranged by size: 5925 1543 945 219 SacI G_AGCT'C Cuts at: 5435 5748 5435 Size: 313 8319 SacII CC_GC'GG Cuts at: 2035 5654 2035 Size: 3619 5013 SalI G'TCGA_C Cuts at: 1936 5974 1936 Size: 4038 4594 SanDI GG'GwC_CC Cuts at: 4319 4319 Size: 8632 SapI GCTCTTCn'nnn_ Cuts at: 5072 5072 Size: 8632 SbfI CC_TGCA'GG Cuts at: 4414 5984 4414 Size: 1570 7062 ScaI AGT'ACT Cuts at: 6880 8592 6880 Size: 1712 6920 SexAI A'CCwGG_T Cuts at: 3150 3848 3150 Size: 698 7934 SfcI C'TryA_G Cuts at: 826 1045 2576 2753 4126 4410 5980 7115 7796 Size: 219 1531 177 1373 284 1570 1135 681 Cuts at: 7796 7987 826 Size: 191 1471 Fragments arranged by size: 1570 1531 1471 1373 1135 681 284 219 191 177 SfiI GGCCn_nnn'nGGCC Cuts at: 4413 4413 Size: 8632 SfoI GGC'GCC Cuts at: 2030 2608 3317 4552 2030 Size: 578 709 1235 6110 Fragments arranged by size: 6110 1235 709 578 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8632 SmaI CCC'GGG Cuts at: 1872 2333 2478 5623 5959 1872 Size: 461 145 3145 336 4545 Fragments arranged by size: 4545 3145 461 336 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 4489 6736 7637 7899 820 Size: 197 926 2546 2247 901 262 1553 Fragments arranged by size: 2546 2247 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6423 493 Size: 5930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8632 SphI G_CATG'C Cuts at: 1132 5990 1132 Size: 4858 3774 SrfI GCCC'GGGC Cuts at: 2478 5623 2478 Size: 3145 5487 SspI AAT'ATT Cuts at: 5 52 6556 5 Size: 47 6504 2081 Fragments arranged by size: 6504 2081 47 StuI AGG'CCT Cuts at: 1517 4573 1517 Size: 3056 5576 StyI C'CwwG_G Cuts at: 513 1065 2004 2178 2547 2926 2979 3699 4568 Size: 552 939 174 369 379 53 720 869 Cuts at: 4568 4600 5160 6071 513 Size: 32 560 911 3074 Fragments arranged by size: 3074 939 911 869 720 560 552 379 369 174 53 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2143 2314 3761 3911 4907 5030 5351 Size: 1255 563 171 1447 150 996 123 321 Cuts at: 5351 5431 6659 6676 6829 7014 7459 325 Size: 80 1228 17 153 185 445 1498 Fragments arranged by size: 1498 1447 1255 1228 996 563 445 321 185 171 153 150 123 80 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2952 3411 3639 Size: 274 80 33 51 1443 975 459 228 Cuts at: 3639 6878 8590 96 Size: 3239 1712 138 Fragments arranged by size: 3239 1712 1443 975 459 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 2433 2465 4171 4190 4203 4222 5330 Size: 32 29 32 1706 19 13 19 1108 Cuts at: 5330 5362 8592 8624 2372 Size: 32 3230 32 2380 Fragments arranged by size: 3230 2380 1706 1108 32 32 32 32 29 19 19 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8632 XbaI T'CTAG_A Cuts at: 5968 5968 Size: 8632 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2560 2986 3690 5167 2560 Size: 426 704 1477 6025 Fragments arranged by size: 6025 1477 704 426 XhoI C'TCGA_G Cuts at: 1943 4489 1943 Size: 2546 6086 XmaI C'CCGG_G Cuts at: 1870 2331 2476 5621 5957 1870 Size: 461 145 3145 336 4545 Fragments arranged by size: 4545 3145 461 336 145 XmnI GAAnn'nnTTC Cuts at: 6761 6761 Size: 8632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4930 5059 6440 276 Size: 53 83 186 936 3396 129 1381 2468 Fragments arranged by size: 3396 2468 1381 936 186 129 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI NruI NsiI PacI PsrI RsrII SwaI