Restriction map for
pFN21AE0112
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbsI BciVI BclI BfrI
BglII BlnI BpmI BpuAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MseI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 69 CGCG CG^CG
AcsI 271 AAATTC [GA]^AATT[CT]
AluI 13 AGCT AG^CT
Aor51HI 568 AGCGCT AGC^GCT
ApaI 508 GGGCCC GGGCC^C
ApoI 271 AAATTC [GA]^AATT[CT]
AspHI 12 GAGCTC G[AT]GC[AT]^C
AvaII 237 GGTCC G^G[AT]CC
BalI 93 TGGCCA TGG^CCA
BanI 394 GGTGCC G^G[CT][GA]CC
BbrPI 97 CACGTG CAC^GTG
BbvCI 220 GCTGAGG CCTCAGC(-5/-2)
BcgI 408 CGAGGGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 571 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 571 GCTGAGC GC^T[ACGT]AGC
BsaAI 97 CACGTG [CT]AC^GT[GA]
BsaI 250 GAGACC GGTCTC(1/5)
BsgI 485 GTGCAG GTGCAG(16/14)
BsiHKAI 12 GAGCTC G[AT]GC[AT]^C
BsmFI 238 GTCCC GGGAC(10/14)
BsrBI 300 GAGCGG CCGCTC(-3/-3)
BsrDI 107 GCAATG GCAATG(2/0)
BsrI 181 ACTGG ACTGG(1/-1)
BstF5I 527 CATCC GGATG(2/0)
BstUI 69 CGCG CG^CG
BstXI 84 CCAGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 571 GCTGAGC GC^T[ACGT]AGC
DpnII 390 GATC ^GATC
Eco47III 568 AGCGCT AGC^GCT
FauI 67 CCCGC CCCGC(4/6)
FokI 527 CATCC GGATG(9/13)
MaeIII 37 GTGAC ^GT[ACGT]AC
MboI 390 GATC ^GATC
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MvnI 69 CGCG CG^CG
NdeII 390 GATC ^GATC
PleI 139 GAGTC GAGTC(4/5)
PmaCI 97 CACGTG CAC^GTG
PmlI 97 CACGTG CAC^GTG
PpuMI 236 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 399 CTGCAG CTGCA^G
SacI 12 GAGCTC GAGCT^C
SapI 370 GAAGAGC GCTCTTC(1/4)
Sau3AI 390 GATC ^GATC
SfaNI 187 GATGC GCATC(5/9)
SmaI 26 CCCGGG CCC^GGG
TaqI 194 TCGA T^CGA
TfiI 343 GATTC G^A[AT]TC
Tsp45I 37 GTGAC ^GT[GC]AC
TthHB8I 194 TCGA T^CGA
XmaCI 26 CCCGGG C^CCGGG
XmaI 26 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Asp700I 267 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
285 GAAGCCTTTC
AvaI 26 CCCGGG C^[CT]CG[GA]G
537 CTCGGG
BanII 12 GAGCTC G[GA]GC[CT]^C
508 GGGCCC
BfaI 209 CTAG C^TAG
482 CTAG
BglI 43 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
466 GCCAAGGGGGC
BseRI 354 CTCCTC GAGGAG(10/8)
46 GAGGAG
BsmAI 113 GAGAC GTCTC(1/5)
250 GAGAC
BsoBI 26 CCCGGG C^[CT]CG[GA]G
537 CTCGGG
CfoI 59 GCGC GCG^C
569 GCGC
EaeI 81 CGGCCA [CT]^GGCC[GA]
93 TGGCCA
EarI 370 GAAGAG CTCTTC(1/4)
499 GAAGAG
HaeII 58 GGCGCT [GA]GCGC^[CT]
568 AGCGCT
HhaI 59 GCGC GCG^C
569 GCGC
HinP1I 59 GCGC G^CGC
569 GCGC
HinfI 139 GAGTC G^A[ACGT]TC
343 GATTC
Ksp632I 370 GAAGAG CTCTTC(1/4)
499 GAAGAG
MaeI 209 CTAG C^TAG
482 CTAG
MaeII 35 ACGT A^CGT
98 ACGT
SfcI 399 CTGCAG C^T[GA][CT]AG
518 CTACAG
Tsp509I 272 AATT ^AATT
296 AATT
TspRI 180 CACTG CA[GC]TG[ACGT][ACGT]^
414 CACTG
XmnI 267 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
285 GAAGCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI BbvI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I Fnu4HI HaeIII HapII
HpaII HphI ItaI MboII MnlI
MspI MvaI MwoI NciI NlaIV
Sau96I ScrFI StyI TseI