Restriction map for
pFN21AE0112
(Linear) (Six-base) MAPSORT of: KIEE0112.seq Check: 9601 from: 1 to: 585
KIEE0112.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AfeI AGC'GCT
Cuts at: 0 570 585
Size: 570 15
ApaI G_GGCC'C
Cuts at: 0 512 585
Size: 512 73
ApoI r'AATT_y
Cuts at: 0 271 585
Size: 271 314
AvaI C'yCGr_G
Cuts at: 0 26 537 585
Size: 26 511 48
Fragments arranged by size:
511 48 26
BanI G'GyrC_C
Cuts at: 0 394 585
Size: 394 191
BanII G_rGCy'C
Cuts at: 0 16 512 585
Size: 16 496 73
Fragments arranged by size:
496 73 16
BbvCI CC'TCA_GC
Cuts at: 0 221 585
Size: 221 364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 431 585
Size: 397 34 154
Fragments arranged by size:
397 154 34
BglI GCCn_nnn'nGGC
Cuts at: 0 49 472 585
Size: 49 423 113
Fragments arranged by size:
423 113 49
BlpI GC'TnA_GC
Cuts at: 0 572 585
Size: 572 13
Bme1580I G_kGCm'C
Cuts at: 0 415 512 585
Size: 415 97 73
Fragments arranged by size:
415 97 73
BmgBI CAC'GTC
Cuts at: 0 36 585
Size: 36 549
Bpu10I CC'TnA_GC
Cuts at: 0 221 419 585
Size: 221 198 166
Fragments arranged by size:
221 198 166
BsaI GGTCTCn'nnnn_
Cuts at: 0 244 585
Size: 244 341
BsaAI yAC'GTr
Cuts at: 0 99 585
Size: 99 486
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 345 585
Size: 61 284 240
Fragments arranged by size:
284 240 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 585
Size: 506 79
BsiHKAI G_wGCw'C
Cuts at: 0 16 585
Size: 16 569
Bsp1286I G_dGCh'C
Cuts at: 0 16 415 512 585
Size: 16 399 97 73
Fragments arranged by size:
399 97 73 16
BsrBI CCG'CTC
Cuts at: 0 302 585
Size: 302 283
BsrDI GCAATG_nn'
Cuts at: 0 114 585
Size: 114 471
BstXI CCAn_nnnn'nTGG
Cuts at: 0 91 585
Size: 91 494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 200 585
Size: 200 385
BtsI GCAGTG_nn'
Cuts at: 0 413 585
Size: 413 172
EaeI y'GGCC_r
Cuts at: 0 81 93 585
Size: 81 12 492
Fragments arranged by size:
492 81 12
EarI CTCTTCn'nnn_
Cuts at: 0 365 494 585
Size: 365 129 91
Fragments arranged by size:
365 129 91
EcoICRI GAG'CTC
Cuts at: 0 14 585
Size: 14 571
EcoO109I rG'GnC_Cy
Cuts at: 0 237 508 509 585
Size: 237 271 1 76
Fragments arranged by size:
271 237 76 1
HaeII r_GCGC'y
Cuts at: 0 62 572 585
Size: 62 510 13
Fragments arranged by size:
510 62 13
MscI TGG'CCA
Cuts at: 0 95 585
Size: 95 490
PmlI CAC'GTG
Cuts at: 0 99 585
Size: 99 486
PpuMI rG'GwC_Cy
Cuts at: 0 237 585
Size: 237 348
PspOMI G'GGCC_C
Cuts at: 0 508 585
Size: 508 77
PstI C_TGCA'G
Cuts at: 0 403 585
Size: 403 182
SacI G_AGCT'C
Cuts at: 0 16 585
Size: 16 569
SapI GCTCTTCn'nnn_
Cuts at: 0 365 585
Size: 365 220
SfcI C'TryA_G
Cuts at: 0 399 518 585
Size: 399 119 67
Fragments arranged by size:
399 119 67
SmaI CCC'GGG
Cuts at: 0 28 585
Size: 28 557
StyI C'CwwG_G
Cuts at: 0 17 125 227 245 467 585
Size: 17 108 102 18 222 118
Fragments arranged by size:
222 118 108 102 18 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 375 585
Size: 375 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 318 350 585
Size: 318 32 235
Fragments arranged by size:
318 235 32
XmaI C'CCGG_G
Cuts at: 0 26 585
Size: 26 559
XmnI GAAnn'nnTTC
Cuts at: 0 271 289 585
Size: 271 18 296
Fragments arranged by size:
296 271 18
Enzymes that do cut:
AfeI ApaI ApoI AvaI BanI BanII BbvCI BcgI
BglI BlpI Bme1580I BmgBI Bpu10I BsaI BsaAI BseRI
BsgI BsiHKAI Bsp1286I BsrBI BsrDI BstXI BtgZI BtsI
EaeI EarI EcoICRI EcoO109I HaeII MscI PmlI PpuMI
PspOMI PstI SacI SapI SfcI SmaI StyI TaqII
TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI
BclI BfrBI BglII BmrI BmtI BplI BpmI BpuEI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI ClaI
CspCI DraI DraIII DrdI EagI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI
ZraI