Restriction map for
pFN21AE0124
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BmyI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MscI MspA1I MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccII 103 CGCG CG^CG
AccIII 86 TCCGGA T^CCGGA
AclI 385 AACGTT AA^CGTT
Asp700I 337 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 71 GGACC G^G[AT]CC
BamHI 643 GGATCC G^GATCC
BfaI 415 CTAG C^TAG
BglII 560 AGATCT A^GATCT
BlpI 692 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 692 GCTCAGC GC^T[ACGT]AGC
BsaWI 86 TCCGGA [AT]^CCGG[AT]
BseAI 86 TCCGGA T^CCGGA
BspEI 86 TCCGGA T^CCGGA
BsrBI 251 GAGCGG CCGCTC(-3/-3)
BsrDI 189 CATTGC GCAATG(2/0)
BstAPI 93 GCACACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 103 CGCG CG^CG
CelII 692 GCTCAGC GC^T[ACGT]AGC
CfoI 102 GCGC GCG^C
DdeI 693 CTCAG C^T[ACGT]AG
DraIII 94 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
HhaI 102 GCGC GCG^C
HinP1I 102 GCGC G^CGC
MaeI 415 CTAG C^TAG
MroI 86 TCCGGA T^CCGGA
MvnI 103 CGCG CG^CG
NspI 275 ACATGC [GA]CATG^[CT]
PflMI 264 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 385 AACGTT AA^CGTT
Van91I 264 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 337 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 313 AAATTT [GA]^AATT[CT]
466 AAATTC
AfaI 751 GTAC GT^AC
978 GTAC
ApoI 313 AAATTT [GA]^AATT[CT]
466 AAATTC
BseRI 539 CTCCTC GAGGAG(10/8)
937 GAGGAG
BsgI 611 GTGCAG GTGCAG(16/14)
868 GTGCAG
BsmFI 70 GGGAC GGGAC(10/14)
24 GTCCC
BsrI 403 CCAGT ACTGG(1/-1)
686 CCAGT
BssSI 907 CACGAG CACGAG(-5/-1)
865 CTCGTG
BstYI 560 AGATCT [GA]^GATC[CT]
643 GGATCC
Cac8I 363 GCAAGC GC[ACGT]^[ACGT]GC
942 GCAAGC
DraI 216 TTTAAA TTT^AAA
352 TTTAAA
FauI 963 CCCGC CCCGC(4/6)
104 GCGGG
HapII 87 CCGG C^CGG
928 CCGG
HpaII 87 CCGG C^CGG
928 CCGG
HphI 553 GGTGA GGTGA(8/7)
585 TCACC
MaeII 386 ACGT A^CGT
426 ACGT
MflI 560 AGATCT [GA]^GATC[CT]
643 GGATCC
MslI 225 CACAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
270 CATGGACATG
MspI 87 CCGG C^CGG
928 CCGG
NlaIV 70 GGGACC GG[ACGT]^[ACGT]CC
643 GGATCC
RsaI 751 GTAC GT^AC
978 GTAC
SapI 922 GAAGAGC GCTCTTC(1/4)
279 GCTCTTC
SfcI 117 CTACAG C^T[GA][CT]AG
506 CTGTAG
XhoII 560 AGATCT [GA]^GATC[CT]
643 GGATCC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BsmAI BspMI BssKI
BstF5I BstNI Cfr13I DpnII EarI
EcoRII Fnu4HI FokI HaeIII ItaI
Ksp632I MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII Sau3AI
Sau96I ScrFI SfaNI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I