(Circular) (Six-base) MAPSORT of: pFN21AE0124.seq Check: 9293 from: 1 to: 5686 pFN21AE0124 5686 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2603 2603 Size: 5686 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3496 278 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3029 1937 Size: 1092 4594 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 2391 3813 4186 1563 Size: 828 1422 373 3063 Fragments arranged by size: 3063 1422 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2886 3749 5133 5401 1632 Size: 1254 863 1384 268 1917 Fragments arranged by size: 1917 1384 1254 863 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 4517 4517 Size: 5686 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 2234 1166 Size: 1068 4618 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5686 AlwNI CAG_nnn'CTG Cuts at: 3429 5001 3429 Size: 1572 4114 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2318 2471 2995 3221 3373 5482 1493 Size: 825 153 524 226 152 2109 1697 Fragments arranged by size: 2109 1697 825 524 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 3011 1870 Size: 73 1068 4545 Fragments arranged by size: 4545 1068 73 BamHI G'GATC_C Cuts at: 2648 3016 3383 2648 Size: 368 367 4951 Fragments arranged by size: 4951 368 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3007 4462 618 Size: 325 217 627 1220 1455 1842 Fragments arranged by size: 1842 1455 1220 627 325 217 BanII G_rGCy'C Cuts at: 1391 3005 1391 Size: 1614 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3877 3911 1903 Size: 34 50 34 1856 34 3678 Fragments arranged by size: 3678 1856 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 1548 Size: 5686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4297 136 Size: 107 122 71 631 605 2625 1525 Fragments arranged by size: 2625 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2565 5681 2565 Size: 3116 2570 BlpI GC'TnA_GC Cuts at: 2698 3103 2698 Size: 405 5281 Bme1580I G_kGCm'C Cuts at: 1790 3747 4903 1790 Size: 1957 1156 2573 Fragments arranged by size: 2573 1957 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3417 4375 442 Size: 416 53 83 399 24 2000 958 1753 Fragments arranged by size: 2000 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4346 1239 Size: 3107 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4349 882 Size: 617 2850 2219 Fragments arranged by size: 2850 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3493 3875 4510 275 Size: 53 83 186 936 1960 382 635 1451 Fragments arranged by size: 1960 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2091 4119 4517 4791 4938 5349 2091 Size: 2028 398 274 147 411 2428 Fragments arranged by size: 2428 2028 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5461 Fragments arranged by size: 5461 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2535 2957 1186 Size: 1349 422 3915 Fragments arranged by size: 3915 1349 422 BseYI C'CCAG_C Cuts at: 4889 5385 4889 Size: 496 5190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2637 2894 1163 Size: 573 901 257 3955 Fragments arranged by size: 3955 901 573 257 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3747 3832 4903 3747 Size: 85 1071 4530 Fragments arranged by size: 4530 1071 85 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3005 3747 3832 4903 1391 Size: 399 1215 742 85 1071 2174 Fragments arranged by size: 2174 1215 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2091 5349 2091 Size: 3258 2428 BspHI T'CATG_A Cuts at: 3575 5305 3575 Size: 1730 3956 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2583 2603 2722 3041 844 Size: 1739 20 119 319 3489 Fragments arranged by size: 3489 1739 319 119 20 BsrBI CCG'CTC Cuts at: 1394 1823 2258 3573 1394 Size: 429 435 1315 3507 Fragments arranged by size: 3507 1315 435 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2193 4181 4355 65 Size: 1183 945 1988 174 1396 Fragments arranged by size: 1988 1396 1183 945 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4330 4517 1700 Size: 110 2520 187 2869 Fragments arranged by size: 2869 2520 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 2870 2912 3746 4758 2870 Size: 42 834 1012 3798 Fragments arranged by size: 3798 1012 834 42 BstAPI GCAn_nnn'nTGC Cuts at: 794 2104 794 Size: 1310 4376 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5686 BstYI r'GATC_y Cuts at: 1972 2565 2648 3016 3383 3768 3785 5237 5323 Size: 593 83 368 367 385 17 1452 86 Cuts at: 5323 5335 5681 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 593 385 368 367 346 86 83 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5134 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4947 Fragments arranged by size: 4947 683 56 BtsI GCAGTG_nn' Cuts at: 3202 3996 4016 3202 Size: 794 20 4872 Fragments arranged by size: 4872 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2223 2359 2990 2223 Size: 136 631 4919 Fragments arranged by size: 4919 631 136 DraIII CAC_nnn'GTG Cuts at: 2104 2104 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4693 809 Size: 507 3377 1802 Fragments arranged by size: 3377 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3133 4022 5651 Size: 54 1425 50 275 18 1303 889 1629 Cuts at: 5651 8 Size: 43 Fragments arranged by size: 1629 1425 1303 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 2922 2973 3616 1353 Size: 938 631 51 643 3423 Fragments arranged by size: 3423 938 643 631 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4273 4647 4793 387 Size: 1064 138 2684 374 146 1280 Fragments arranged by size: 2684 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2886 3749 4346 5133 5401 1239 Size: 393 1254 863 597 787 268 1524 Fragments arranged by size: 1524 1254 863 787 597 393 268 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2703 2703 Size: 5686 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 4833 1678 Size: 3155 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2175 2201 2207 2233 4327 Size: 32 135 32 603 26 6 26 2094 Cuts at: 4327 4359 4401 4433 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2094 603 135 42 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3864 Fragments arranged by size: 3864 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2234 2279 3644 4003 4162 518 Size: 648 1068 45 1365 359 159 2042 Fragments arranged by size: 2042 1365 1068 648 359 159 45 MspA1I CmG'CkG Cuts at: 1606 3102 3779 4927 5172 1606 Size: 1496 677 1148 245 2120 Fragments arranged by size: 2120 1496 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5134 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 2284 3044 4589 1132 Size: 97 1055 760 1545 2229 Fragments arranged by size: 2229 1545 1055 760 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5686 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 1108 Size: 1167 4519 PfoI T'CCnGG_A Cuts at: 2061 2436 2061 Size: 375 5311 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PstI C_TGCA'G Cuts at: 830 3038 830 Size: 2208 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SapI GCTCTTCn'nnn_ Cuts at: 2291 2922 2291 Size: 631 5055 SbfI CC_TGCA'GG Cuts at: 3038 3038 Size: 5686 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SfcI C'TryA_G Cuts at: 826 1045 2122 2511 3034 4169 4850 5041 826 Size: 219 1077 389 523 1135 681 191 1471 Fragments arranged by size: 1471 1135 1077 681 523 389 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5686 SmaI CCC'GGG Cuts at: 1872 3013 1872 Size: 1141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 3044 1132 Size: 1912 3774 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5686 StyI C'CwwG_G Cuts at: 513 1065 3125 513 Size: 552 2060 3074 Fragments arranged by size: 3074 2060 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3713 3730 3883 4068 4513 325 Size: 1255 2133 17 153 185 445 1498 Fragments arranged by size: 2133 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3932 5644 96 Size: 274 80 33 51 1443 1955 1712 138 Fragments arranged by size: 1955 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5646 5678 5646 Size: 32 5654 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 3011 1870 Size: 1141 4545 XmnI GAAnn'nnTTC Cuts at: 2346 3815 2346 Size: 1469 4217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3494 276 Size: 53 83 186 936 1960 2468 Fragments arranged by size: 2468 1960 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI