(Circular) (Six-base) MAPSORT of: pFN21AB6529.seq Check: 1412 from: 1 to: 5800 pFN21AB6529 5800 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2046 3610 278 Size: 53 83 186 936 510 1564 2468 Fragments arranged by size: 2468 1564 936 510 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 2565 3121 2565 Size: 556 5244 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 2477 2516 3034 3863 5247 5515 1632 Size: 620 225 39 518 829 1384 268 1917 Fragments arranged by size: 1917 1384 829 620 518 268 225 39 AflII C'TTAA_G Cuts at: 820 1017 2417 820 Size: 197 1400 4203 Fragments arranged by size: 4203 1400 197 AflIII A'CryG_T Cuts at: 1203 2079 4699 5676 1203 Size: 876 2620 977 1327 Fragments arranged by size: 2620 1327 977 876 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 2140 2623 4529 2140 Size: 483 1906 3411 Fragments arranged by size: 3411 1906 483 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AlwNI CAG_nnn'CTG Cuts at: 2392 2710 2752 2788 3543 5115 2392 Size: 318 42 36 755 1572 3077 Fragments arranged by size: 3077 1572 755 318 42 36 ApaLI G'TGCA_C Cuts at: 2444 3857 5013 2444 Size: 1413 1156 3231 Fragments arranged by size: 3231 1413 1156 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 2576 3125 1870 Size: 73 633 549 4545 Fragments arranged by size: 4545 633 549 73 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2565 2725 3078 3121 4576 Size: 325 217 627 778 160 353 43 1455 Cuts at: 4576 618 Size: 1842 Fragments arranged by size: 1842 1455 778 627 353 325 217 160 43 BanII G_rGCy'C Cuts at: 1391 2265 2575 3119 1391 Size: 874 310 544 4072 Fragments arranged by size: 4072 874 544 310 BbeI G_GCGC'C Cuts at: 3082 3082 Size: 5800 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2266 928 Size: 971 367 4462 Fragments arranged by size: 4462 971 367 BbvCI CC'TCA_GC Cuts at: 2588 2588 Size: 5800 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2906 3087 4411 Size: 107 122 71 631 605 1234 181 1324 Cuts at: 4411 136 Size: 1525 Fragments arranged by size: 1525 1324 1234 631 605 181 122 107 71 BglII A'GATC_T Cuts at: 2193 5795 2193 Size: 3602 2198 BlpI GC'TnA_GC Cuts at: 2171 3217 2171 Size: 1046 4754 Bme1580I G_kGCm'C Cuts at: 1790 2448 3861 5017 1790 Size: 658 1413 1156 2573 Fragments arranged by size: 2573 1413 1156 658 BmgBI CAC'GTC Cuts at: 1318 2095 2881 1318 Size: 777 786 4237 Fragments arranged by size: 4237 786 777 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3531 4489 442 Size: 416 53 83 399 24 2114 958 1753 Fragments arranged by size: 2114 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2879 2891 4460 1239 Size: 1640 12 1569 2579 Fragments arranged by size: 2579 1640 1569 12 Bpu10I CC'TnA_GC Cuts at: 2588 2588 Size: 5800 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3925 4790 5088 3925 Size: 865 298 4637 Fragments arranged by size: 4637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4463 882 Size: 617 2964 2219 Fragments arranged by size: 2964 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2080 2551 3591 493 Size: 711 876 471 1040 2702 Fragments arranged by size: 2702 1040 876 711 471 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2532 3079 3607 Size: 53 83 186 936 510 489 547 528 Cuts at: 3607 3989 4624 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 547 528 510 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2016 4233 4631 4905 5052 5463 2016 Size: 2217 398 274 147 411 2353 Fragments arranged by size: 2353 2217 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5575 Fragments arranged by size: 5575 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2278 2390 1186 Size: 1092 112 4596 Fragments arranged by size: 4596 1092 112 BseYI C'CCAG_C Cuts at: 2281 2435 2964 5003 5499 2281 Size: 154 529 2039 496 2582 Fragments arranged by size: 2582 2039 529 496 154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2765 1163 Size: 573 1029 4198 Fragments arranged by size: 4198 1029 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2901 3250 4011 5039 1540 Size: 275 186 900 349 761 1028 2301 Fragments arranged by size: 2301 1028 900 761 349 275 186 BsiHKAI G_wGCw'C Cuts at: 2126 2448 3861 3946 5017 2126 Size: 322 1413 85 1071 2909 Fragments arranged by size: 2909 1413 1071 322 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 BsmBI CGTCTCn'nnnn_ Cuts at: 2948 2948 Size: 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2265 2448 2575 3119 3861 3946 Size: 399 336 139 183 127 544 742 85 Cuts at: 3946 5017 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 544 399 336 183 139 127 85 BspEI T'CCGG_A Cuts at: 2016 5463 2016 Size: 3447 2353 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2862 3155 844 Size: 2018 293 3489 Fragments arranged by size: 3489 2018 293 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3057 4295 4469 65 Size: 1183 1809 1238 174 1396 Fragments arranged by size: 1809 1396 1238 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4444 4631 1700 Size: 110 2634 187 2869 Fragments arranged by size: 2869 2634 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 2206 1925 Size: 281 5519 BssSI C'ACGA_G Cuts at: 2993 3860 4872 2993 Size: 867 1012 3921 Fragments arranged by size: 3921 1012 867 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 2554 1184 Size: 1370 4430 BstXI CCAn_nnnn'nTGG Cuts at: 2435 2435 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2193 2341 2382 2706 3130 3497 3882 3899 Size: 221 148 41 324 424 367 385 17 Cuts at: 3899 5351 5437 5449 5795 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 424 385 367 346 324 221 148 86 41 17 12 Bsu36I CC'TnA_GG Cuts at: 2946 2946 Size: 5800 BtgI C'CryG_G Cuts at: 513 1065 2828 513 Size: 552 1763 3485 Fragments arranged by size: 3485 1763 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 3316 4110 4130 3316 Size: 794 20 4986 Fragments arranged by size: 4986 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2080 4807 809 Size: 507 764 2727 1802 Fragments arranged by size: 2727 1802 764 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2157 2766 Size: 54 1425 50 275 18 297 30 609 Cuts at: 2766 2898 3247 4136 5765 8 Size: 132 349 889 1629 43 Fragments arranged by size: 1629 1425 889 609 349 297 275 132 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2898 3247 1537 Size: 275 1086 349 4090 Fragments arranged by size: 4090 1086 349 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2946 3730 1353 Size: 1593 784 3423 Fragments arranged by size: 3423 1593 784 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2477 2516 2879 2891 3034 3863 Size: 393 620 225 39 363 12 143 829 Cuts at: 3863 4460 5247 5515 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 829 787 620 597 393 363 268 225 143 39 12 EcoO109I rG'GnC_Cy Cuts at: 2106 2452 2908 2941 2106 Size: 346 456 33 4965 Fragments arranged by size: 4965 456 346 33 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FspI TGC'GCA Cuts at: 2297 4306 2297 Size: 2009 3791 HaeII r_GCGC'y Cuts at: 1678 2893 3082 4947 1678 Size: 1215 189 1865 2531 Fragments arranged by size: 2531 1865 1215 189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2126 2158 2609 2641 4441 Size: 32 135 32 554 32 451 32 1800 Cuts at: 4441 4473 4515 4547 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1800 554 451 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2047 3144 3390 669 Size: 988 281 109 1097 246 3079 Fragments arranged by size: 3079 1097 988 281 246 109 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KasI G'GCGC_C Cuts at: 3078 3078 Size: 5800 KpnI G_GTAC'C Cuts at: 2569 3125 2569 Size: 556 5244 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4914 5098 1305 Size: 3609 184 2007 Fragments arranged by size: 3609 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2159 10 Size: 54 1425 343 327 3651 Fragments arranged by size: 3651 1425 343 327 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 3758 4117 4276 518 Size: 648 1194 1398 359 159 2042 Fragments arranged by size: 2042 1398 1194 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2103 2562 2592 2698 2821 3216 3893 5041 Size: 497 459 30 106 123 395 677 1148 Cuts at: 5041 5286 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 497 459 395 245 123 106 30 NarI GG'CG_CC Cuts at: 3079 3079 Size: 5800 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5248 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 2242 2528 3158 4703 1132 Size: 97 1013 286 630 1545 2229 Fragments arranged by size: 2229 1545 1013 630 286 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 2392 2614 1108 Size: 1284 222 4294 Fragments arranged by size: 4294 1284 222 PfoI T'CCnGG_A Cuts at: 2265 2937 2265 Size: 672 5128 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2941 2452 Size: 489 5311 PshAI GACnn'nnGTC Cuts at: 1613 2093 1613 Size: 480 5320 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PstI C_TGCA'G Cuts at: 830 2349 3152 830 Size: 1519 803 3478 Fragments arranged by size: 3478 1519 803 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 PvuII CAG'CTG Cuts at: 2592 2698 2821 2592 Size: 106 123 5571 Fragments arranged by size: 5571 123 106 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SanDI GG'GwC_CC Cuts at: 2941 2941 Size: 5800 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SexAI A'CCwGG_T Cuts at: 2316 2391 2316 Size: 75 5725 SfcI C'TryA_G Cuts at: 826 1045 2345 3148 4283 4964 5155 826 Size: 219 1300 803 1135 681 191 1471 Fragments arranged by size: 1471 1300 1135 803 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2906 2906 Size: 5800 SfoI GGC'GCC Cuts at: 3080 3080 Size: 5800 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2417 3904 4805 5067 820 Size: 197 926 474 1487 901 262 1553 Fragments arranged by size: 1553 1487 926 901 474 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2415 1517 Size: 898 4902 StyI C'CwwG_G Cuts at: 513 1065 3239 513 Size: 552 2174 3074 Fragments arranged by size: 3074 2174 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2930 3827 3844 3997 4182 4627 325 Size: 1255 1350 897 17 153 185 445 1498 Fragments arranged by size: 1498 1350 1255 897 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 4046 5758 Size: 274 80 33 51 1443 444 1625 1712 Cuts at: 5758 96 Size: 138 Fragments arranged by size: 1712 1625 1443 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5760 5792 5760 Size: 32 5768 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 2810 3929 2810 Size: 1119 4681 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2044 3608 276 Size: 53 83 186 936 510 1564 2468 Fragments arranged by size: 2468 1564 936 510 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SrfI SwaI XcmI