Restriction map for
pFN21AA1878
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbrPI
BbsI BciVI BclI BfrI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MaeII MaeIII MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AcsI 910 GAATTC [GA]^AATT[CT]
AlwNI 254 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 605 AGCGCT AGC^GCT
ApoI 910 GAATTC [GA]^AATT[CT]
AscI 833 GGCGCGCC GG^CGCGCC
BalI 610 TGGCCA TGG^CCA
BamHI 518 GGATCC G^GATCC
BcgI 222 CGAGTTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1130 CTAG C^TAG
BglII 536 AGATCT A^GATCT
BseRI 871 GAGGAG GAGGAG(10/8)
BsmBI 202 GAGACG CGTCTC(1/5)
BsmFI 506 GGGAC GGGAC(10/14)
BspHI 993 TCATGA T^CATGA
DrdI 1051 GACAATAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 605 AGCGCT AGC^GCT
EcoRI 910 GAATTC G^AATTC
HinfI 352 GACTC G^A[ACGT]TC
MaeI 1130 CTAG C^TAG
MboII 1073 GAAGA GAAGA(8/7)
MluNI 610 TGGCCA TGG^CCA
MscI 610 TGGCCA TGG^CCA
MslI 69 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 253 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 352 GACTC GAGTC(4/5)
RcaI 993 TCATGA T^CATGA
ScaI 95 AGTACT AGT^ACT
TaqI 449 TCGA T^CGA
Tsp509I 911 AATT ^AATT
TthHB8I 449 TCGA T^CGA
Van91I 253 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 519 GATCC GGATC(4/5)
518 GGATC
Asp700I 471 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
906 GAATGAATTC
AspHI 112 GAGCAC G[AT]GC[AT]^C
1010 GTGCTC
AvaII 507 GGACC G^G[AT]CC
801 GGTCC
BsaI 457 GAGACC GGTCTC(1/5)
997 GAGACC
BsiHKAI 112 GAGCAC G[AT]GC[AT]^C
1010 GTGCTC
BsrBI 303 GAGCGG CCGCTC(-3/-3)
662 GAGCGG
BstF5I 228 GGATG GGATG(2/0)
737 GGATG
BstYI 518 GGATCC [GA]^GATC[CT]
536 AGATCT
FokI 228 GGATG GGATG(9/13)
737 GGATG
HphI 357 GGTGA GGTGA(8/7)
89 TCACC
MflI 518 GGATCC [GA]^GATC[CT]
536 AGATCT
NlaIII 920 CATG CATG^
994 CATG
PvuII 405 CAGCTG CAG^CTG
1046 CAGCTG
XhoII 518 GGATCC [GA]^GATC[CT]
536 AGATCT
XmnI 471 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
906 GAATGAATTC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AvaI BanI BanII BbeI
BbvCI BbvI BcnI BglI BmyI
BsaHI BsaJI BsgI BsiEI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrFI
BsrI BssHII BssKI BssSI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI KasI KspI MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIV RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
TseI TspRI XmaCI XmaI