(Circular) (Six-base) MAPSORT of: pFN21AA1878.seq Check: 6629 from: 1 to: 6022 pFN21AA1878 6022 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3365 1937 Size: 1428 4594 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4085 5469 5737 1632 Size: 2453 1384 268 1917 Fragments arranged by size: 2453 1917 1384 268 AfeI AGC'GCT Cuts at: 2612 2612 Size: 6022 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 4921 5898 1203 Size: 3718 977 1327 Fragments arranged by size: 3718 1327 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6022 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2562 1184 Size: 1378 4644 AlwNI CAG_nnn'CTG Cuts at: 2264 3765 5337 2264 Size: 1501 1572 2949 Fragments arranged by size: 2949 1572 1501 ApaI G_GGCC'C Cuts at: 2300 2321 3251 2300 Size: 21 930 5071 Fragments arranged by size: 5071 930 21 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 2915 3331 3557 3709 5818 1493 Size: 1422 416 226 152 2109 1697 Fragments arranged by size: 2109 1697 1422 416 226 152 AscI GG'CGCG_CC Cuts at: 2839 2839 Size: 6022 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 2122 2244 2320 2402 2576 2640 2822 Size: 73 179 122 76 82 174 64 182 Cuts at: 2822 3250 3347 1870 Size: 428 97 4545 Fragments arranged by size: 4545 428 182 179 174 122 97 82 76 73 64 BamHI G'GATC_C Cuts at: 2523 3352 3719 2523 Size: 829 367 4826 Fragments arranged by size: 4826 829 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 2566 2581 3343 4798 Size: 325 217 627 339 440 15 762 1455 Cuts at: 4798 618 Size: 1842 Fragments arranged by size: 1842 1455 762 627 440 339 325 217 15 BanII G_rGCy'C Cuts at: 1391 2300 2321 2333 2409 3251 3341 1391 Size: 909 21 12 76 842 90 4072 Fragments arranged by size: 4072 909 842 90 76 21 12 BbeI G_GCGC'C Cuts at: 2130 2570 2585 2130 Size: 440 15 5567 Fragments arranged by size: 5567 440 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5051 BbvCI CC'TCA_GC Cuts at: 2634 3166 3309 2634 Size: 532 143 5347 Fragments arranged by size: 5347 532 143 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2216 2250 4213 4247 1903 Size: 34 279 34 1963 34 3678 Fragments arranged by size: 3678 1963 279 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 1548 Size: 6022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2558 2636 2690 Size: 107 122 71 631 605 886 78 54 Cuts at: 2690 4633 136 Size: 1943 1525 Fragments arranged by size: 1943 1525 886 631 605 122 107 78 71 54 BglII A'GATC_T Cuts at: 2541 6017 2541 Size: 3476 2546 BlpI GC'TnA_GC Cuts at: 3439 3439 Size: 6022 Bme1580I G_kGCm'C Cuts at: 1790 2300 2321 2772 3251 4083 5239 1790 Size: 510 21 451 479 832 1156 2573 Fragments arranged by size: 2573 1156 832 510 479 451 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6022 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2461 2962 3753 Size: 416 53 83 399 24 1044 501 791 Cuts at: 3753 4711 442 Size: 958 1753 Fragments arranged by size: 1753 1044 958 791 501 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4682 1239 Size: 3443 2579 Bpu10I CC'TnA_GC Cuts at: 2634 3166 3309 2634 Size: 532 143 5347 Fragments arranged by size: 5347 532 143 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4147 5012 5310 4147 Size: 865 298 4859 Fragments arranged by size: 4859 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2456 2996 4685 882 Size: 617 957 540 1689 2219 Fragments arranged by size: 2219 1689 957 617 540 BsaAI yAC'GTr Cuts at: 493 1204 3813 493 Size: 711 2609 2702 Fragments arranged by size: 2702 2609 711 BsaBI GATnn'nnATC Cuts at: 6016 6016 Size: 6022 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2127 2561 2567 2582 Size: 53 83 186 936 594 434 6 15 Cuts at: 2582 2693 2780 2853 3829 4211 4846 275 Size: 111 87 73 976 382 635 1451 Fragments arranged by size: 1451 976 936 635 594 434 382 186 111 87 83 73 53 15 6 BsaWI w'CCGG_w Cuts at: 4455 4853 5127 5274 5685 4455 Size: 398 274 147 411 4792 Fragments arranged by size: 4792 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2453 1178 Size: 30 165 30 1020 30 4747 Fragments arranged by size: 4747 1020 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2891 1186 Size: 1705 4317 BseYI C'CCAG_C Cuts at: 2408 5225 5721 2408 Size: 2817 496 2709 Fragments arranged by size: 2817 2709 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2414 2558 2642 2910 1163 Size: 573 678 144 84 268 4275 Fragments arranged by size: 4275 678 573 268 144 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2305 2420 2597 2685 3472 4233 Size: 275 186 304 115 177 88 787 761 Cuts at: 4233 5261 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 787 761 304 275 186 177 115 88 BsiHKAI G_wGCw'C Cuts at: 2121 3019 4083 4168 5239 2121 Size: 898 1064 85 1071 2904 Fragments arranged by size: 2904 1071 1064 898 85 BsmI GAATG_Cn' Cuts at: 3533 3626 3533 Size: 93 5929 BsmBI CGTCTCn'nnnn_ Cuts at: 2201 2201 Size: 6022 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2300 2321 2333 2409 2772 3019 Size: 399 331 179 21 12 76 363 247 Cuts at: 3019 3251 3341 4083 4168 5239 1391 Size: 232 90 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 363 331 247 232 179 90 85 76 21 12 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 2998 3911 5641 2998 Size: 913 1730 3379 Fragments arranged by size: 3379 1730 913 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3377 844 Size: 2533 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2310 2669 3909 1394 Size: 429 487 359 1240 3507 Fragments arranged by size: 3507 1240 487 429 359 BsrDI GCAATG_nn' Cuts at: 65 1248 4517 4691 65 Size: 1183 3269 174 1396 Fragments arranged by size: 3269 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2167 2273 2287 2415 2592 2816 2862 Size: 110 357 106 14 128 177 224 46 Cuts at: 2862 4666 4853 1700 Size: 1804 187 2869 Fragments arranged by size: 2869 1804 357 224 187 177 128 110 106 46 14 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6022 BssHII G'CGCG_C Cuts at: 1925 2189 2191 2839 1925 Size: 264 2 648 5108 Fragments arranged by size: 5108 648 264 2 BssSI C'ACGA_G Cuts at: 2023 2771 3184 4082 5094 2023 Size: 748 413 898 1012 2951 Fragments arranged by size: 2951 1012 898 748 413 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6022 BstBI TT'CG_AA Cuts at: 4826 4826 Size: 6022 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6022 BstYI r'GATC_y Cuts at: 1972 2523 2541 3352 3719 4104 4121 5573 5659 Size: 551 18 811 367 385 17 1452 86 Cuts at: 5659 5671 6017 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 811 551 385 367 346 86 18 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2352 2506 2519 2790 2844 513 Size: 552 939 348 154 13 271 54 3691 Fragments arranged by size: 3691 939 552 348 271 154 54 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2023 2059 487 Size: 683 56 797 36 4450 Fragments arranged by size: 4450 797 683 56 36 BtsI GCAGTG_nn' Cuts at: 2898 3538 4332 4352 2898 Size: 640 794 20 4568 Fragments arranged by size: 4568 794 640 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6022 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5987 DraI TTT'AAA Cuts at: 3326 3326 Size: 6022 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3062 5029 809 Size: 507 1746 1967 1802 Fragments arranged by size: 1967 1802 1746 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 2302 2417 Size: 54 1425 50 275 18 182 290 115 Cuts at: 2417 2492 2594 2615 2682 3469 4358 5987 8 Size: 75 102 21 67 787 889 1629 43 Fragments arranged by size: 1629 1425 889 787 290 275 182 115 102 75 67 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2302 2417 2594 2682 3469 1537 Size: 275 490 115 177 88 787 4090 Fragments arranged by size: 4090 787 490 275 177 115 88 EarI CTCTTCn'nnn_ Cuts at: 1353 3952 1353 Size: 2599 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2679 2717 2864 4609 4983 5129 Size: 1064 138 1090 38 147 1745 374 146 Cuts at: 5129 387 Size: 1280 Fragments arranged by size: 1745 1280 1090 1064 374 147 146 138 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4085 4682 5469 5737 1239 Size: 393 2453 597 787 268 1524 Fragments arranged by size: 2453 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2028 2297 2761 3247 2028 Size: 269 464 486 4803 Fragments arranged by size: 4803 486 464 269 EcoRI G'AATT_C Cuts at: 2915 3331 3709 2915 Size: 416 378 5228 Fragments arranged by size: 5228 416 378 FspI TGC'GCA Cuts at: 4528 4528 Size: 6022 HaeII r_GCGC'y Cuts at: 1678 2130 2174 2264 2570 2585 2593 2614 5169 Size: 452 44 90 306 15 8 21 2555 Cuts at: 5169 1678 Size: 2531 Fragments arranged by size: 2555 2531 452 306 90 44 21 15 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4663 4695 4737 4769 1373 Size: 32 135 32 3091 32 42 32 2626 Fragments arranged by size: 3091 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3366 3612 669 Size: 988 281 1428 246 3079 Fragments arranged by size: 3079 1428 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KasI G'GCGC_C Cuts at: 2126 2566 2581 2126 Size: 440 15 5567 Fragments arranged by size: 5567 440 15 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2040 2211 2466 5136 5320 1305 Size: 735 171 255 2670 184 2007 Fragments arranged by size: 2670 2007 735 255 184 171 MscI TGG'CCA Cuts at: 10 64 1489 1832 2617 10 Size: 54 1425 343 785 3415 Fragments arranged by size: 3415 1425 785 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 3980 4339 4498 518 Size: 648 912 1902 359 159 2042 Fragments arranged by size: 2042 1902 912 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2086 2354 2398 2412 2497 2508 2633 2785 Size: 480 268 44 14 85 11 125 152 Cuts at: 2785 2792 2846 3053 3438 4115 5263 5508 1606 Size: 7 54 207 385 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 480 385 268 245 207 152 125 85 54 44 14 11 7 NaeI GCC'GGC Cuts at: 2169 2289 2417 2594 2169 Size: 120 128 177 5597 Fragments arranged by size: 5597 177 128 120 NarI GG'CG_CC Cuts at: 2127 2567 2582 2127 Size: 440 15 5567 Fragments arranged by size: 5567 440 15 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4531 Fragments arranged by size: 4531 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NgoMIV G'CCGG_C Cuts at: 2167 2287 2415 2592 2167 Size: 120 128 177 5597 Fragments arranged by size: 5597 177 128 120 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 3380 4925 1132 Size: 97 2151 1545 2229 Fragments arranged by size: 2229 2151 1545 97 PasI CC'CwG_GG Cuts at: 1348 2221 1348 Size: 873 5149 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2264 1108 Size: 902 254 4866 Fragments arranged by size: 4866 902 254 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4123 5604 5636 4091 Size: 32 1481 32 4477 Fragments arranged by size: 4477 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 3592 3592 Size: 6022 PspOMI G'GGCC_C Cuts at: 2296 2317 3247 2296 Size: 21 930 5071 Fragments arranged by size: 5071 930 21 PstI C_TGCA'G Cuts at: 830 3374 830 Size: 2544 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 PvuII CAG'CTG Cuts at: 2412 3053 2412 Size: 641 5381 SacII CC_GC'GG Cuts at: 2355 2509 2793 2847 2355 Size: 154 284 54 5530 Fragments arranged by size: 5530 284 154 54 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 2102 4270 5982 2102 Size: 2168 1712 2142 Fragments arranged by size: 2168 2142 1712 SfcI C'TryA_G Cuts at: 826 1045 2905 3010 3094 3370 4505 5186 5377 Size: 219 1860 105 84 276 1135 681 191 Cuts at: 5377 826 Size: 1471 Fragments arranged by size: 1860 1471 1135 681 276 219 191 105 84 SfoI GGC'GCC Cuts at: 2128 2568 2583 2128 Size: 440 15 5567 Fragments arranged by size: 5567 440 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 2124 2246 2322 2642 3349 1872 Size: 252 122 76 320 707 4545 Fragments arranged by size: 4545 707 320 252 122 76 SmlI C'TyrA_G Cuts at: 820 1017 1943 4126 5027 5289 820 Size: 197 926 2183 901 262 1553 Fragments arranged by size: 2183 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3813 493 Size: 3320 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 3380 1132 Size: 2248 3774 SrfI GCCC'GGGC Cuts at: 2246 2322 2246 Size: 76 5946 SspI AAT'ATT Cuts at: 5 52 3946 5 Size: 47 3894 2081 Fragments arranged by size: 3894 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6022 StyI C'CwwG_G Cuts at: 513 1065 2004 3461 513 Size: 552 939 1457 3074 Fragments arranged by size: 3074 1457 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4049 4066 4219 4404 4849 325 Size: 1255 2469 17 153 185 445 1498 Fragments arranged by size: 2469 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2100 2970 4268 Size: 274 80 33 51 1443 123 870 1298 Cuts at: 4268 5980 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1298 870 274 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2504 2536 5982 6014 2504 Size: 32 3446 32 2512 Fragments arranged by size: 3446 2512 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 2122 2244 2320 2640 3347 1870 Size: 252 122 76 320 707 4545 Fragments arranged by size: 4545 707 320 252 122 76 XmnI GAAnn'nnTTC Cuts at: 2480 2915 4151 2480 Size: 435 1236 4351 Fragments arranged by size: 4351 1236 435 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI