Restriction map for
pFN21AB7339
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# NO CUT ENZYMES ( 87 enzymes) #
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AccI AflII AflIII AgeI Aor51HI
AscI AseI AsnI AspI AviII
AvrII BbeI BbrPI BbsI BcgI
BclI BfrI BglI BglII BlnI
BpuAI BsaBI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eco47III EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1907 GGTACC G^GTACC
AclI 1648 AACGTT AA^CGTT
Alw44I 652 GTGCAC G^TGCAC
AocI 1089 CCTCAGG CC^T[ACGT]AGG
ApaLI 652 GTGCAC G^TGCAC
Asp700I 1920 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1907 GGTACC G^GTACC
BalI 497 TGGCCA TGG^CCA
BanI 1907 GGTACC G^G[CT][GA]CC
BbvCI 2465 CCTCAGC CCTCAGC(-5/-2)
BlpI 1257 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1257 GCTGAGC GC^T[ACGT]AGC
BsmBI 1882 GAGACG CGTCTC(1/5)
BspHI 677 TCATGA T^CATGA
BsrFI 206 GCCGGC [GA]^CCGG[CT]
BssHII 2081 GCGCGC G^CGCGC
BstAPI 2304 GCATGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1089 CCTCAGG CC^T[ACGT]AGG
CelII 1257 GCTGAGC GC^T[ACGT]AGC
Cfr10I 206 GCCGGC [GA]^CCGG[CT]
DraIII 2065 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1962 GACGCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1089 CCTCAGG CC^T[ACGT]AGG
EcoNI 174 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 274 GAATTC G^AATTC
FauI 2011 CCCGC CCCGC(4/6)
HaeII 790 AGCGCC [GA]GCGC^[CT]
KpnI 1907 GGTACC GGTAC^C
MluNI 497 TGGCCA TGG^CCA
MscI 497 TGGCCA TGG^CCA
MseI 95 TTAA T^TAA
NaeI 206 GCCGGC GCC^GGC
NcoI 231 CCATGG C^CATGG
NgoMIV 206 GCCGGC G^CCGGC
PflMI 366 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1648 AACGTT AA^CGTT
PstI 1166 CTGCAG CTGCA^G
RcaI 677 TCATGA T^CATGA
SapI 2058 GCTCTTC GCTCTTC(1/4)
SexAI 122 ACCTGGT A^CC[AT]GGT
Tru9I 95 TTAA T^TAA
Van91I 366 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1920 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AatII 4 GACGTC GACGT^C
1884 GACGTC
AccII 1980 CGCG CG^CG
2082 CGCG
AccIII 540 TCCGGA T^CCGGA
1487 TCCGGA
AcsI 274 GAATTC [GA]^AATT[CT]
1924 AAATTC
AfaI 1635 GTAC GT^AC
1908 GTAC
ApaI 1291 GGGCCC GGGCC^C
1857 GGGCCC
ApoI 274 GAATTC [GA]^AATT[CT]
1924 AAATTC
BamHI 983 GGATCC G^GATCC
1490 GGATCC
BciVI 571 GGATAC GTATCC(6/5)
820 GTATCC
BfaI 1616 CTAG C^TAG
2326 CTAG
BsaAI 78 CACGTA [CT]AC^GT[GA]
1626 TACGTG
BseAI 540 TCCGGA T^CCGGA
1487 TCCGGA
BsiEI 1502 CGGCCG CG[GA][CT]^CG
2041 CGGCCG
BspEI 540 TCCGGA T^CCGGA
1487 TCCGGA
BstEII 2136 GGTGACC G^GT[ACGT]ACC
2193 GGTGACC
BstPI 2136 GGTGACC G^GT[ACGT]ACC
2193 GGTGACC
BstUI 1980 CGCG CG^CG
2082 CGCG
DsaI 231 CCATGG C^C[GA][CT]GG
2264 CCGTGG
EagI 1502 CGGCCG C^GGCCG
2041 CGGCCG
EclXI 1502 CGGCCG C^GGCCG
2041 CGGCCG
Eco52I 1502 CGGCCG C^GGCCG
2041 CGGCCG
EcoO65I 2136 GGTGACC G^GT[ACGT]ACC
2193 GGTGACC
HgaI 503 GACGC GACGC(5/10)
1962 GACGC
MaeI 1616 CTAG C^TAG
2326 CTAG
MroI 540 TCCGGA T^CCGGA
1487 TCCGGA
MslI 795 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2022 CATCGGCGTG
MvnI 1980 CGCG CG^CG
2082 CGCG
PaeR7I 101 CTCGAG C^TCGAG
1082 CTCGAG
PleI 771 GAGTC GAGTC(4/5)
1123 GAGTC
PshAI 997 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1222 GACGAGGGTC
RsaI 1635 GTAC GT^AC
1908 GTAC
SacI 331 GAGCTC GAGCT^C
1848 GAGCTC
SmlI 101 CTCGAG C^T[CT][GA]AG
1082 CTCGAG
TfiI 837 GAATC G^A[AT]TC
876 GAATC
XhoI 101 CTCGAG C^TCGAG
1082 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcyI AhdI AluI AlwI
AlwNI AspEI AspHI AvaI AvaII
BanII BbvI BcnI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI Eam1105I EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI