Restriction map for
pFN21AB7339
(Linear) (Six-base) MAPSORT of: KIBB7339.seq Check: 1632 from: 1 to: 2499
KIBB7339.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1547 1932 2124 2304 2499
Size: 1547 385 192 180 195
Fragments arranged by size:
1547 385 195 192 180
AatII G_ACGT'C
Cuts at: 0 8 1888 2499
Size: 8 1880 611
Fragments arranged by size:
1880 611 8
Acc65I G'GTAC_C
Cuts at: 0 1907 2499
Size: 1907 592
AclI AA'CG_TT
Cuts at: 0 1649 2499
Size: 1649 850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 160 583 730 892 1885 2272 2499
Size: 154 6 423 147 162 993 387 227
Fragments arranged by size:
993 423 387 227 162 154 147 6
AhdI GACnn_n'nnGTC
Cuts at: 0 111 594 770 2499
Size: 111 483 176 1729
Fragments arranged by size:
1729 483 176 111
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1369 1401 1774 1806 2499
Size: 1369 32 373 32 693
Fragments arranged by size:
1369 693 373 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 66 372 1539 1947 2002 2352 2499
Size: 66 306 1167 408 55 350 147
Fragments arranged by size:
1167 408 350 306 147 66 55
ApaI G_GGCC'C
Cuts at: 0 1295 1861 2499
Size: 1295 566 638
Fragments arranged by size:
1295 638 566
ApaLI G'TGCA_C
Cuts at: 0 652 2499
Size: 652 1847
ApoI r'AATT_y
Cuts at: 0 274 1924 2499
Size: 274 1650 575
Fragments arranged by size:
1650 575 274
AvaI C'yCGr_G
Cuts at: 0 38 101 880 922 1082 1263 1388 2035
Size: 38 63 779 42 160 181 125 647
Cuts at: 2035 2474 2499
Size: 439 25
Fragments arranged by size:
779 647 439 181 160 125 63 42
38 25
BamHI G'GATC_C
Cuts at: 0 983 1490 2499
Size: 983 507 1009
Fragments arranged by size:
1009 983 507
BanI G'GyrC_C
Cuts at: 0 1907 2499
Size: 1907 592
BanII G_rGCy'C
Cuts at: 0 335 923 1264 1295 1852 1861 2035 2325
Size: 335 588 341 31 557 9 174 290
Cuts at: 2325 2499
Size: 174
Fragments arranged by size:
588 557 341 335 290 174 174 31
9
BbvCI CC'TCA_GC
Cuts at: 0 2466 2499
Size: 2466 33
BciVI GTATCCnnnnn_n'
Cuts at: 0 565 831 2499
Size: 565 266 1668
Fragments arranged by size:
1668 565 266
BlpI GC'TnA_GC
Cuts at: 0 1258 2499
Size: 1258 1241
Bme1580I G_kGCm'C
Cuts at: 0 656 1295 1455 1861 2499
Size: 656 639 160 406 638
Fragments arranged by size:
656 639 638 406 160
BmgBI CAC'GTC
Cuts at: 0 1149 2499
Size: 1149 1350
BmrI ACTGGGnnnn_n'
Cuts at: 0 2206 2499
Size: 2206 293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 319 349 954 2419 2499
Size: 280 39 30 605 1465 80
Fragments arranged by size:
1465 605 280 80 39 30
Bpu10I CC'TnA_GC
Cuts at: 0 2466 2499
Size: 2466 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 26 1036 1079 1717 2351 2499
Size: 26 1010 43 638 634 148
Fragments arranged by size:
1010 638 634 148 43 26
BsaAI yAC'GTr
Cuts at: 0 80 1628 2499
Size: 80 1548 871
Fragments arranged by size:
1548 871 80
BsaHI Gr'CG_yC
Cuts at: 0 5 504 1885 1963 2499
Size: 5 499 1381 78 536
Fragments arranged by size:
1381 536 499 78 5
BsaWI w'CCGG_w
Cuts at: 0 540 1277 1487 1577 2499
Size: 540 737 210 90 922
Fragments arranged by size:
922 737 540 210 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1167 1197 1369 1399 2300 2330 2499
Size: 1167 30 172 30 901 30 169
Fragments arranged by size:
1167 901 172 169 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 931 1070 1172 2425 2428 2499
Size: 931 139 102 1253 3 71
Fragments arranged by size:
1253 931 139 102 71 3
BseYI C'CCAG_C
Cuts at: 0 66 442 787 824 1053 2097 2482 2499
Size: 66 376 345 37 229 1044 385 17
Fragments arranged by size:
1044 385 376 345 229 66 37 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2152 2332 2424 2499
Size: 2152 180 92 75
Fragments arranged by size:
2152 180 92 75
BsiEI CG_ry'CG
Cuts at: 0 1505 2044 2499
Size: 1505 539 455
Fragments arranged by size:
1505 539 455
BsiHKAI G_wGCw'C
Cuts at: 0 335 365 656 1852 2060 2113 2120 2499
Size: 335 30 291 1196 208 53 7 379
Fragments arranged by size:
1196 379 335 291 208 53 30 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1876 2499
Size: 1876 623
Bsp1286I G_dGCh'C
Cuts at: 0 335 365 656 923 1264 1295 1455 1852
Size: 335 30 291 267 341 31 160 397
Cuts at: 1852 1861 2035 2060 2113 2120 2325 2499
Size: 9 174 25 53 7 205 174
Fragments arranged by size:
397 341 335 291 267 205 174 174
160 53 31 30 25 9 7
BspEI T'CCGG_A
Cuts at: 0 540 1487 2499
Size: 540 947 1012
Fragments arranged by size:
1012 947 540
BspHI T'CATG_A
Cuts at: 0 677 2499
Size: 677 1822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 1547 1932 2124 2304 2499
Size: 62 1485 385 192 180 195
Fragments arranged by size:
1485 385 195 192 180 62
BsrBI CCG'CTC
Cuts at: 0 642 968 1066 2499
Size: 642 326 98 1433
Fragments arranged by size:
1433 642 326 98
BsrFI r'CCGG_y
Cuts at: 0 206 2499
Size: 206 2293
BssHII G'CGCG_C
Cuts at: 0 2081 2499
Size: 2081 418
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2310 2499
Size: 2310 189
BstEII G'GTnAC_C
Cuts at: 0 2136 2193 2499
Size: 2136 57 306
Fragments arranged by size:
2136 306 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 222 621 978 2499
Size: 222 399 357 1521
Fragments arranged by size:
1521 399 357 222
BstYI r'GATC_y
Cuts at: 0 263 855 955 983 1490 1799 2237 2499
Size: 263 592 100 28 507 309 438 262
Fragments arranged by size:
592 507 438 309 263 262 100 28
Bsu36I CC'TnA_GG
Cuts at: 0 1090 2499
Size: 1090 1409
BtgI C'CryG_G
Cuts at: 0 231 2264 2499
Size: 231 2033 235
Fragments arranged by size:
2033 235 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1728 2499
Size: 1728 771
BtsI GCAGTG_nn'
Cuts at: 0 1954 2412 2424 2499
Size: 1954 458 12 75
Fragments arranged by size:
1954 458 75 12
DraIII CAC_nnn'GTG
Cuts at: 0 2070 2499
Size: 2070 429
DrdI GACnn_nn'nnGTC
Cuts at: 0 1968 2499
Size: 1968 531
EaeI y'GGCC_r
Cuts at: 0 497 611 623 1502 2041 2499
Size: 497 114 12 879 539 458
Fragments arranged by size:
879 539 497 458 114 12
EagI C'GGCC_G
Cuts at: 0 1502 2041 2499
Size: 1502 539 458
Fragments arranged by size:
1502 539 458
EarI CTCTTCn'nnn_
Cuts at: 0 676 1432 2065 2499
Size: 676 756 633 434
Fragments arranged by size:
756 676 633 434
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 963 1852 2499
Size: 963 889 647
Fragments arranged by size:
963 889 647
EcoICRI GAG'CTC
Cuts at: 0 333 1850 2499
Size: 333 1517 649
Fragments arranged by size:
1517 649 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 160 280 319 349 583 730 892
Size: 154 6 120 39 30 234 147 162
Cuts at: 892 954 1885 2272 2419 2499
Size: 62 931 387 147 80
Fragments arranged by size:
931 387 234 162 154 147 147 120
80 62 39 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 178 2499
Size: 178 2321
EcoO109I rG'GnC_Cy
Cuts at: 0 120 889 1228 1292 1857 2125 2207 2499
Size: 120 769 339 64 565 268 82 292
Fragments arranged by size:
769 565 339 292 268 120 82 64
EcoRI G'AATT_C
Cuts at: 0 274 2499
Size: 274 2225
HaeII r_GCGC'y
Cuts at: 0 794 2499
Size: 794 1705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 151 183 410 442 580 612
Size: 97 32 22 32 227 32 138 32
Cuts at: 612 756 788 792 824 833 840 865 872
Size: 144 32 4 32 9 7 25 7
Cuts at: 872 2406 2438 2499
Size: 1534 32 61
Fragments arranged by size:
1534 227 144 138 97 61 32 32
32 32 32 32 32 25 22 9
7 7 4
KpnI G_GTAC'C
Cuts at: 0 1911 2499
Size: 1911 588
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2256 2499
Size: 2256 243
MscI TGG'CCA
Cuts at: 0 499 2499
Size: 499 2000
MslI CAynn'nnrTG
Cuts at: 0 799 2026 2499
Size: 799 1227 473
Fragments arranged by size:
1227 799 473
MspA1I CmG'CkG
Cuts at: 0 63 553 1257 2014 2340 2499
Size: 63 490 704 757 326 159
Fragments arranged by size:
757 704 490 326 159 63
NaeI GCC'GGC
Cuts at: 0 208 2499
Size: 208 2291
NcoI C'CATG_G
Cuts at: 0 231 2499
Size: 231 2268
NgoMIV G'CCGG_C
Cuts at: 0 206 2499
Size: 206 2293
PasI CC'CwG_GG
Cuts at: 0 600 2499
Size: 600 1899
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 2499
Size: 372 2127
PfoI T'CCnGG_A
Cuts at: 0 296 851 1802 2499
Size: 296 555 951 697
Fragments arranged by size:
951 697 555 296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1355 1369 1387 1401 2499
Size: 1355 14 18 14 1098
Fragments arranged by size:
1355 1098 18 14 14
PpuMI rG'GwC_Cy
Cuts at: 0 120 889 1228 2125 2207 2499
Size: 120 769 339 897 82 292
Fragments arranged by size:
897 769 339 292 120 82
PshAI GACnn'nnGTC
Cuts at: 0 1001 1226 2499
Size: 1001 225 1273
Fragments arranged by size:
1273 1001 225
PspOMI G'GGCC_C
Cuts at: 0 1291 1857 2499
Size: 1291 566 642
Fragments arranged by size:
1291 642 566
PstI C_TGCA'G
Cuts at: 0 1170 2499
Size: 1170 1329
PvuII CAG'CTG
Cuts at: 0 553 1257 2340 2499
Size: 553 704 1083 159
Fragments arranged by size:
1083 704 553 159
SacI G_AGCT'C
Cuts at: 0 335 1852 2499
Size: 335 1517 647
Fragments arranged by size:
1517 647 335
SanDI GG'GwC_CC
Cuts at: 0 2125 2207 2499
Size: 2125 82 292
Fragments arranged by size:
2125 292 82
SapI GCTCTTCn'nnn_
Cuts at: 0 2065 2499
Size: 2065 434
SexAI A'CCwGG_T
Cuts at: 0 122 2499
Size: 122 2377
SfcI C'TryA_G
Cuts at: 0 58 631 1072 1166 2344 2499
Size: 58 573 441 94 1178 155
Fragments arranged by size:
1178 573 441 155 94 58
SmaI CCC'GGG
Cuts at: 0 40 882 1265 1390 2037 2499
Size: 40 842 383 125 647 462
Fragments arranged by size:
842 647 462 383 125 40
SmlI C'TyrA_G
Cuts at: 0 101 1082 2499
Size: 101 981 1417
Fragments arranged by size:
1417 981 101
StuI AGG'CCT
Cuts at: 0 845 2293 2495 2499
Size: 845 1448 202 4
Fragments arranged by size:
1448 845 202 4
StyI C'CwwG_G
Cuts at: 0 231 1526 1701 2499
Size: 231 1295 175 798
Fragments arranged by size:
1295 798 231 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 976 1620 2263 2499
Size: 58 918 644 643 236
Fragments arranged by size:
918 644 643 236 58
TatI w'GTAC_w
Cuts at: 0 1634 2499
Size: 1634 865
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1167 1199 2222 2254 2300 2332 2499
Size: 1167 32 1023 32 46 32 167
Fragments arranged by size:
1167 1023 167 46 32 32 32
XhoI C'TCGA_G
Cuts at: 0 101 1082 2499
Size: 101 981 1417
Fragments arranged by size:
1417 981 101
XmaI C'CCGG_G
Cuts at: 0 38 880 1263 1388 2035 2499
Size: 38 842 383 125 647 464
Fragments arranged by size:
842 647 464 383 125 38
XmnI GAAnn'nnTTC
Cuts at: 0 1924 2499
Size: 1924 575
ZraI GAC'GTC
Cuts at: 0 6 1886 2499
Size: 6 1880 613
Fragments arranged by size:
1880 613 6
Enzymes that do cut:
AarI AatII Acc65I AclI AcuI AhdI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbvCI
BciVI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KpnI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SanDI SapI SexAI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AfeI AflII AflIII AgeI AleI AlfI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BcgI BclI
BfrBI BglI BglII BmtI BplI BpuEI BsaBI BsiWI
BsmI BsrDI BsrGI BssSI BstBI BstZ17I ClaI CspCI
DraI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XcmI