Restriction map for
pFN21AB5785
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaWI BseAI BsiWI BspDI
BspEI BspHI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MluI MroI MvnI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SacII SalI SbfI ScaI SexAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 2067 GACGTC GACGT^C
Alw44I 1898 GTGCAC G^TGCAC
Aor51HI 818 AGCGCT AGC^GCT
ApaLI 1898 GTGCAC G^TGCAC
BamHI 1205 GGATCC G^GATCC
BbeI 1478 GGCGCC GGCGC^C
BbrPI 1084 CACGTG CAC^GTG
BbsI 350 GAAGAC GAAGAC(2/6)
BbvCI 390 GCTGAGG CCTCAGC(-5/-2)
BpuAI 350 GAAGAC GAAGAC(2/6)
BsaAI 1084 CACGTG [CT]AC^GT[GA]
BsaBI 951 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1523 GAGACC GGTCTC(1/5)
BsgI 2197 GTGCAG GTGCAG(16/14)
BsiEI 956 CGATCG CG[GA][CT]^CG
BsmBI 2065 GAGACG CGTCTC(1/5)
BsmI 1888 GCATTC GAATGC(1/-1)
Bsp1407I 545 TGTACA T^GTACA
BsrBI 2171 CCGCTC CCGCTC(-3/-3)
BsrDI 1455 CATTGC GCAATG(2/0)
BsrGI 545 TGTACA T^GTACA
BstBI 2261 TTCGAA TT^CGAA
DraIII 1081 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 818 AGCGCT AGC^GCT
HgaI 1993 GACGC GACGC(5/10)
HindIII 178 AAGCTT A^AGCTT
KasI 1478 GGCGCC G^GCGCC
MamI 951 GATGGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1617 CAATTG C^AATTG
MunI 1617 CAATTG C^AATTG
NaeI 1475 GCCGGC GCC^GGC
NarI 1478 GGCGCC GG^CGCC
NgoMIV 1475 GCCGGC G^CCGGC
NspV 2261 TTCGAA TT^CGAA
PflMI 1080 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1084 CACGTG CAC^GTG
PmlI 1084 CACGTG CAC^GTG
PpuMI 79 GGGTCCC [GA]G^G[AT]CC[CT]
PvuI 956 CGATCG CGAT^CG
SapI 489 GCTCTTC GCTCTTC(1/4)
SfoI 1478 GGCGCC GGC^GCC
SfuI 2261 TTCGAA TT^CGAA
SgrAI 1474 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 768 CTTGAG C^T[CT][GA]AG
SspBI 545 TGTACA T^GTACA
TfiI 1752 GATTC G^A[AT]TC
Van91I 1080 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 934 GAATTC [GA]^AATT[CT]
1220 GAATTC
AcyI 1478 GGCGCC G[GA]^CG[CT]C
2067 GACGTC
AflIII 542 ACATGT A^C[GA][CT]GT
2126 ACATGT
AlwNI 1003 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2311 CAGCTGCTG
AocI 133 CCTGAGG CC^T[ACGT]AGG
326 CCTCAGG
ApoI 934 GAATTC [GA]^AATT[CT]
1220 GAATTC
AvaI 1049 CCCGAG C^[CT]CG[GA]G
1210 CCCGAG
BalI 428 TGGCCA TGG^CCA
971 TGGCCA
BanI 214 GGCACC G^G[CT][GA]CC
1478 GGCGCC
BcnI 774 CCCGG CC^[GC]GG
1202 CCCGG
BfaI 206 CTAG C^TAG
268 CTAG
BsaHI 1478 GGCGCC G[GA]^CG[CT]C
2067 GACGTC
BseRI 295 GAGGAG GAGGAG(10/8)
2299 GAGGAG
BsoBI 1049 CCCGAG C^[CT]CG[GA]G
1210 CCCGAG
BspLU11I 542 ACATGT A^CATGT
2126 ACATGT
BspMI 279 ACCTGC ACCTGC(4/8)
1949 ACCTGC
BsrFI 485 ACCGGC [GA]^CCGG[CT]
1475 GCCGGC
Bsu36I 133 CCTGAGG CC^T[ACGT]AGG
326 CCTCAGG
Cfr10I 485 ACCGGC [GA]^CCGG[CT]
1475 GCCGGC
DraII 79 GGGTCCC [GA]G^G[ACGT]CC[CT]
649 GGGGCCT
EarI 490 CTCTTC CTCTTC(1/4)
750 GAAGAG
Eco81I 133 CCTGAGG CC^T[ACGT]AGG
326 CCTCAGG
EcoNI 781 CCTGATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
800 CCTCCAAAAGG
EcoO109I 79 GGGTCCC [GA]G^G[ACGT]CC[CT]
649 GGGGCCT
EcoRI 934 GAATTC G^AATTC
1220 GAATTC
HaeII 818 AGCGCT [GA]GCGC^[CT]
1478 GGCGCC
Hin1I 1478 GGCGCC G[GA]^CG[CT]C
2067 GACGTC
Ksp632I 490 CTCTTC CTCTTC(1/4)
750 GAAGAG
MaeI 206 CTAG C^TAG
268 CTAG
MluNI 428 TGGCCA TGG^CCA
971 TGGCCA
MscI 428 TGGCCA TGG^CCA
971 TGGCCA
NciI 774 CCCGG CC^[GC]GG
1202 CCCGG
NcoI 425 CCATGG C^CATGG
2244 CCATGG
SacI 1018 GAGCTC GAGCT^C
1363 GAGCTC
SfiI 141 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
315 GGCCGCTGTGGCC
StuI 149 AGGCCT AGG^CCT
1325 AGGCCT
Tsp45I 1391 GTGAC ^GT[GC]AC
1830 GTCAC
XcmI 2013 CCACATCGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2087 CCACAGAGTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BglI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII