Restriction map for
pFN21AB5785
(Circular) (Six-base) MAPSORT of: pFN21AB5785.seq Check: 8782 from: 1 to: 7144
pFN21AB5785 7144 bp
With 182 enzymes: SgfI *
March 8, 2011 15:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2329 2329
Size: 7144
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4112 4954 278
Size: 53 83 186 936 2576 842 2468
Fragments arranged by size:
2576 2468 936 842 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4487 1937
Size: 2550 4594
Acc65I G'GTAC_C
Cuts at: 4465 4465
Size: 7144
AclI AA'CG_TT
Cuts at: 1563 5271 5644 1563
Size: 3708 373 3063
Fragments arranged by size:
3708 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2685 3316 5207 6591 6859 1632
Size: 1053 631 1891 1384 268 1917
Fragments arranged by size:
1917 1891 1384 1053 631 268
AfeI AGC'GCT
Cuts at: 2861 2861
Size: 7144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6947
AflIII A'CryG_T
Cuts at: 1203 2583 4167 6043 7020 1203
Size: 1380 1584 1876 977 1327
Fragments arranged by size:
1876 1584 1380 1327 977
AgeI A'CCGG_T
Cuts at: 5975 5975
Size: 7144
AhdI GACnn_n'nnGTC
Cuts at: 5873 5873
Size: 7144
AleI CACnn'nnGTG
Cuts at: 1166 2733 1166
Size: 1567 5577
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3971 3981 1184
Size: 2787 10 4347
Fragments arranged by size:
4347 2787 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3476 3508 3476
Size: 32 7112
AlwNI CAG_nnn'CTG
Cuts at: 3049 4357 4887 6459 3049
Size: 1308 530 1572 3734
Fragments arranged by size:
3734 1572 1308 530
ApaLI G'TGCA_C
Cuts at: 3939 5201 6357 3939
Size: 1262 1156 4726
Fragments arranged by size:
4726 1262 1156
ApoI r'AATT_y
Cuts at: 1493 2975 3261 4453 4679 4831 6940 1493
Size: 1482 286 1192 226 152 2109 1697
Fragments arranged by size:
2109 1697 1482 1192 286 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5698 160
Size: 872 4666 1606
Fragments arranged by size:
4666 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
AvaI C'yCGr_G
Cuts at: 1870 1943 3090 3251 4469 1870
Size: 73 1147 161 1218 4545
Fragments arranged by size:
4545 1218 1147 161 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2040 2073 2040
Size: 33 7111
BamHI G'GATC_C
Cuts at: 3246 4474 4841 3246
Size: 1228 367 5549
Fragments arranged by size:
5549 1228 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 3519 4465 5920 618
Size: 325 217 627 468 1264 946 1455 1842
Fragments arranged by size:
1842 1455 1264 946 627 468 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2816 3063 3408 3603 3638 4463 1391
Size: 700 725 247 345 195 35 825 4072
Fragments arranged by size:
4072 825 725 700 345 247 195 35
BbeI G_GCGC'C
Cuts at: 3523 3523
Size: 7144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2398 928
Size: 971 499 5674
Fragments arranged by size:
5674 971 499
BbvCI CC'TCA_GC
Cuts at: 2432 2432
Size: 7144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5335 5369 1903
Size: 34 3398 34 3678
Fragments arranged by size:
3678 3398 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5036 6246 5036
Size: 1210 5934
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2189 2363 4161
Size: 107 122 71 631 605 517 174 1798
Cuts at: 4161 5755 136
Size: 1594 1525
Fragments arranged by size:
1798 1594 1525 631 605 517 174 122
107 71
BglII A'GATC_T
Cuts at: 7139 7139
Size: 7144
BlpI GC'TnA_GC
Cuts at: 4561 4561
Size: 7144
Bme1580I G_kGCm'C
Cuts at: 1790 2680 3345 3771 3943 5205 6361 1790
Size: 890 665 426 172 1262 1156 2573
Fragments arranged by size:
2573 1262 1156 890 665 426 172
BmgBI CAC'GTC
Cuts at: 1318 2554 2608 1318
Size: 1236 54 5854
Fragments arranged by size:
5854 1236 54
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2566 3439 4875
Size: 416 53 83 399 24 1149 873 1436
Cuts at: 4875 5833 442
Size: 958 1753
Fragments arranged by size:
1753 1436 1149 958 873 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7144
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 7144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2523 2654 4063 4276 5804 1239
Size: 1046 238 131 1409 213 1528 2579
Fragments arranged by size:
2579 1528 1409 1046 238 213 131
Bpu10I CC'TnA_GC
Cuts at: 2432 2432
Size: 7144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2830 5269 6134 6432 2830
Size: 2439 865 298 3542
Fragments arranged by size:
3542 2439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3558 5807 882
Size: 617 2059 2249 2219
Fragments arranged by size:
2249 2219 2059 617
BsaAI yAC'GTr
Cuts at: 493 1204 3127 4935 493
Size: 711 1923 1808 2702
Fragments arranged by size:
2702 1923 1808 711
BsaBI GATnn'nnATC
Cuts at: 2996 7138 2996
Size: 4142 3002
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3520 4109 4951 5333
Size: 53 83 186 936 1987 589 842 382
Cuts at: 5333 5968 275
Size: 635 1451
Fragments arranged by size:
1987 1451 936 842 635 589 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5577 5975 6249 6396 6807 5577
Size: 398 274 147 411 5914
Fragments arranged by size:
5914 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2037 2067 3425 3455 3476
Size: 30 165 30 634 30 1358 30 21
Cuts at: 3476 3506 1178
Size: 30 4816
Fragments arranged by size:
4816 1358 634 165 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 4355 1186
Size: 1165 2004 3975
Fragments arranged by size:
3975 2004 1165
BseYI C'CCAG_C
Cuts at: 3968 6347 6843 3968
Size: 2379 496 4269
Fragments arranged by size:
4269 2379 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4259 1163
Size: 573 2523 4048
Fragments arranged by size:
4048 2523 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3000 4594 5355 6383 1540
Size: 275 186 999 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 999 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3063 3408 3909 3943 5205 5290 6361 3063
Size: 345 501 34 1262 85 1071 3846
Fragments arranged by size:
3846 1262 1071 501 345 85 34
BsmI GAATG_Cn'
Cuts at: 3929 4655 4748 3929
Size: 726 93 6325
Fragments arranged by size:
6325 726 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4100 4100
Size: 7144
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2680 2816 3063 3345 3408 3603
Size: 399 301 589 136 247 282 63 195
Cuts at: 3603 3638 3771 3909 3943 4463 5205 5290 6361
Size: 35 133 138 34 520 742 85 1071
Cuts at: 6361 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 589 520 399 301 282
247 195 138 136 133 85 63 35
34
BspEI T'CCGG_A
Cuts at: 6807 6807
Size: 7144
BspHI T'CATG_A
Cuts at: 5033 6763 5033
Size: 1730 5414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2329 3999 4499 844
Size: 1485 1670 500 3489
Fragments arranged by size:
3489 1670 1485 500
BsrBI CCG'CTC
Cuts at: 1394 1823 4214 5031 1394
Size: 429 2391 817 3507
Fragments arranged by size:
3507 2391 817 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3495 5639 5813 65
Size: 1183 2247 2144 174 1396
Fragments arranged by size:
2247 2144 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2526 3516 5788 5975 1700
Size: 110 716 990 2272 187 2869
Fragments arranged by size:
2869 2272 990 716 187 110
BsrGI T'GTAC_A
Cuts at: 96 2586 96
Size: 2490 4654
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7144
BssSI C'ACGA_G
Cuts at: 5204 6216 5204
Size: 1012 6132
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7144
BstBI TT'CG_AA
Cuts at: 4303 5948 4303
Size: 1645 5499
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7144
BstYI r'GATC_y
Cuts at: 1972 2298 3246 3574 4474 4841 5226 5243 6695
Size: 326 948 328 900 367 385 17 1452
Cuts at: 6695 6781 6793 7139 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 948 900 385 367 346 328
326 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2175 2368 2175
Size: 193 6951
BtgI C'CryG_G
Cuts at: 513 1065 2040 2466 2734 4285 513
Size: 552 975 426 268 1551 3372
Fragments arranged by size:
3372 1551 975 552 426 268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6405
Fragments arranged by size:
6405 683 56
BtsI GCAGTG_nn'
Cuts at: 2877 3105 4660 5454 5474 2877
Size: 228 1555 794 20 4547
Fragments arranged by size:
4547 1555 794 228 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2250 2285 4117 4152 529
Size: 35 1686 35 1832 35 3521
Fragments arranged by size:
3521 1832 1686 35 35 35
DraI TTT'AAA
Cuts at: 4448 4448
Size: 7144
DraIII CAC_nnn'GTG
Cuts at: 3127 3127
Size: 7144
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6151 809
Size: 507 4835 1802
Fragments arranged by size:
4835 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2469 3012
Size: 54 1425 50 275 18 207 432 543
Cuts at: 3012 3646 4591 5480 7109 8
Size: 634 945 889 1629 43
Fragments arranged by size:
1629 1425 945 889 634 543 432 275
207 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4591 1537
Size: 275 2779 4090
Fragments arranged by size:
4090 2779 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2537 2786 5074 1353
Size: 1184 249 2288 3423
Fragments arranged by size:
3423 2288 1184 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3503 3637 5731 6105 6251 387
Size: 1064 138 1914 134 2094 374 146 1280
Fragments arranged by size:
2094 1914 1280 1064 374 146 138 134
EcoICRI GAG'CTC
Cuts at: 3061 3406 3061
Size: 345 6799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2523 2654 2685 3316 4063 4276
Size: 393 653 238 131 31 631 747 213
Cuts at: 4276 5207 5804 6591 6859 1239
Size: 931 597 787 268 1524
Fragments arranged by size:
1524 931 787 747 653 631 597 393
268 238 213 131 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2826 2845 2826
Size: 19 7125
EcoO109I rG'GnC_Cy
Cuts at: 2121 2691 2121
Size: 570 6574
EcoRI G'AATT_C
Cuts at: 2975 3261 4453 4831 2975
Size: 286 1192 378 5288
Fragments arranged by size:
5288 1192 378 286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2237 2237
Size: 7144
FspI TGC'GCA
Cuts at: 5650 5650
Size: 7144
HaeII r_GCGC'y
Cuts at: 1678 2863 3523 6291 1678
Size: 1185 660 2768 2531
Fragments arranged by size:
2768 2531 1185 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2964 2996 3356 3388 3425
Size: 32 135 32 1392 32 360 32 37
Cuts at: 3425 3457 4198 4230 5785 5817 5859 5891 1373
Size: 32 741 32 1555 32 42 32 2626
Fragments arranged by size:
2626 1555 1392 741 360 135 42 37
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4488 4734 669
Size: 988 281 2550 246 3079
Fragments arranged by size:
3079 2550 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2219 748
Size: 1471 5673
HpaI GTT'AAC
Cuts at: 4734 4734
Size: 7144
KasI G'GCGC_C
Cuts at: 3519 3519
Size: 7144
KpnI G_GTAC'C
Cuts at: 4469 4469
Size: 7144
MfeI C'AATT_G
Cuts at: 3658 4743 3658
Size: 1085 6059
MluI A'CGCG_T
Cuts at: 7020 7020
Size: 7144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2187 6258 6442 1305
Size: 882 4071 184 2007
Fragments arranged by size:
4071 2007 882 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 2471 3014 10
Size: 54 1425 343 207 432 543 4140
Fragments arranged by size:
4140 1425 543 432 343 207 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 2594 2598 2733 4331 5102 5461
Size: 648 1299 129 4 135 1598 771 359
Cuts at: 5461 5620 518
Size: 159 2042
Fragments arranged by size:
2042 1598 1299 771 648 359 159 135
129 4
MspA1I CmG'CkG
Cuts at: 1606 2360 3429 3535 4348 4354 4560 5237 6385
Size: 754 1069 106 813 6 206 677 1148
Cuts at: 6385 6630 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1069 813 754 677 245 206
106 6
NaeI GCC'GGC
Cuts at: 3518 3518
Size: 7144
NarI GG'CG_CC
Cuts at: 3520 3520
Size: 7144
NcoI C'CATG_G
Cuts at: 513 1065 2040 2466 4285 513
Size: 552 975 426 1819 3372
Fragments arranged by size:
3372 1819 975 552 426
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7144
NgoMIV G'CCGG_C
Cuts at: 3516 3516
Size: 7144
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7144
NotI GC'GGCC_GC
Cuts at: 4591 4591
Size: 7144
NspI r_CATG'y
Cuts at: 1132 1229 2587 2710 4171 4502 6047 1132
Size: 97 1358 123 1461 331 1545 2229
Fragments arranged by size:
2229 1545 1461 1358 331 123 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7144
PciI A'CATG_T
Cuts at: 2583 4167 6043 2583
Size: 1584 1876 3684
Fragments arranged by size:
3684 1876 1584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3127 1108
Size: 2019 5125
PmeI GTTT'AAAC
Cuts at: 4448 4448
Size: 7144
PmlI CAC'GTG
Cuts at: 3127 3127
Size: 7144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 5213 5245 6726 6758 3476
Size: 32 1705 32 1481 32 3862
Fragments arranged by size:
3862 1705 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2121 2121
Size: 7144
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7144
PsiI TTA'TAA
Cuts at: 4714 4714
Size: 7144
PstI C_TGCA'G
Cuts at: 830 3586 3744 4353 4496 830
Size: 2756 158 609 143 3478
Fragments arranged by size:
3478 2756 609 158 143
PvuI CG_AT'CG
Cuts at: 2001 3000 2001
Size: 999 6145
PvuII CAG'CTG
Cuts at: 3429 3535 4348 4354 3429
Size: 106 813 6 6219
Fragments arranged by size:
6219 813 106 6
SacI G_AGCT'C
Cuts at: 3063 3408 3063
Size: 345 6799
SalI G'TCGA_C
Cuts at: 1936 4486 1936
Size: 2550 4594
SanDI GG'GwC_CC
Cuts at: 2121 2121
Size: 7144
SapI GCTCTTCn'nnn_
Cuts at: 2537 2537
Size: 7144
SbfI CC_TGCA'GG
Cuts at: 4496 4496
Size: 7144
ScaI AGT'ACT
Cuts at: 5392 7104 5392
Size: 1712 5432
SfcI C'TryA_G
Cuts at: 826 1045 3582 3740 3899 4349 4492 5627 6308
Size: 219 2537 158 159 450 143 1135 681
Cuts at: 6308 6499 826
Size: 191 1471
Fragments arranged by size:
2537 1471 1135 681 450 219 191 159
158 143
SfiI GGCCn_nnn'nGGCC
Cuts at: 2189 2363 2189
Size: 174 6970
SfoI GGC'GCC
Cuts at: 3521 3521
Size: 7144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7144
SgrAI Cr'CCGG_yG
Cuts at: 1700 3516 1700
Size: 1816 5328
SmaI CCC'GGG
Cuts at: 1872 4471 1872
Size: 2599 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2809 5248 6149 6411 820
Size: 197 926 866 2439 901 262 1553
Fragments arranged by size:
2439 1553 926 901 866 262 197
SnaBI TAC'GTA
Cuts at: 493 4935 493
Size: 4442 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7144
SphI G_CATG'C
Cuts at: 1132 4502 1132
Size: 3370 3774
SspI AAT'ATT
Cuts at: 5 52 5068 5
Size: 47 5016 2081
Fragments arranged by size:
5016 2081 47
StuI AGG'CCT
Cuts at: 1517 2192 3368 1517
Size: 675 1176 5293
Fragments arranged by size:
5293 1176 675
StyI C'CwwG_G
Cuts at: 513 1065 2040 2466 2493 3168 4285 4583 513
Size: 552 975 426 27 675 1117 298 3074
Fragments arranged by size:
3074 1117 975 675 552 426 298 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 5171 5188 5341 5526 5971 325
Size: 1255 1020 2571 17 153 185 445 1498
Fragments arranged by size:
2571 1498 1255 1020 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2506 2586 5390
Size: 274 80 33 51 1443 529 80 2804
Cuts at: 5390 7102 96
Size: 1712 138
Fragments arranged by size:
2804 1712 1443 529 274 138 80 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7104 7136 7104
Size: 32 7112
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7144
XbaI T'CTAG_A
Cuts at: 4480 4480
Size: 7144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 4061 4135 4438 2011
Size: 2050 74 303 4717
Fragments arranged by size:
4717 2050 303 74
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7144
XmaI C'CCGG_G
Cuts at: 1870 4469 1870
Size: 2599 4545
XmnI GAAnn'nnTTC
Cuts at: 5273 5273
Size: 7144
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4110 4952 276
Size: 53 83 186 936 2576 842 2468
Fragments arranged by size:
2576 2468 936 842 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BfrBI BsiWI BstXI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI
PsrI RsrII SacII SexAI SrfI SwaI