(Linear) (Six-base) MAPSORT of: KIBB5785.seq Check: 643 from: 1 to: 2403 KIBB5785.orf With 182 enzymes: SgfI * March 8, 2011 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 288 2403 Size: 288 2115 AatII G_ACGT'C Cuts at: 0 2071 2403 Size: 2071 332 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 644 1275 2403 Size: 644 631 1128 Fragments arranged by size: 1128 644 631 AfeI AGC'GCT Cuts at: 0 820 2403 Size: 820 1583 AflIII A'CryG_T Cuts at: 0 542 2126 2403 Size: 542 1584 277 Fragments arranged by size: 1584 542 277 AleI CACnn'nnGTG Cuts at: 0 692 2403 Size: 692 1711 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1930 1940 2403 Size: 1930 10 463 Fragments arranged by size: 1930 463 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1435 1467 2403 Size: 1435 32 936 Fragments arranged by size: 1435 936 32 AlwNI CAG_nnn'CTG Cuts at: 0 1008 2316 2403 Size: 1008 1308 87 Fragments arranged by size: 1308 1008 87 ApaLI G'TGCA_C Cuts at: 0 1898 2403 Size: 1898 505 ApoI r'AATT_y Cuts at: 0 934 1220 2403 Size: 934 286 1183 Fragments arranged by size: 1183 934 286 AvaI C'yCGr_G Cuts at: 0 1049 1210 2403 Size: 1049 161 1193 Fragments arranged by size: 1193 1049 161 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 32 2403 Size: 32 2371 BamHI G'GATC_C Cuts at: 0 1205 2403 Size: 1205 1198 BanI G'GyrC_C Cuts at: 0 214 1478 2403 Size: 214 1264 925 Fragments arranged by size: 1264 925 214 BanII G_rGCy'C Cuts at: 0 50 775 1022 1367 1562 1597 2403 Size: 50 725 247 345 195 35 806 Fragments arranged by size: 806 725 345 247 195 50 35 BbeI G_GCGC'C Cuts at: 0 1482 2403 Size: 1482 921 BbsI GAAGACnn'nnnn_ Cuts at: 0 357 2403 Size: 357 2046 BbvCI CC'TCA_GC Cuts at: 0 391 2403 Size: 391 2012 BglI GCCn_nnn'nGGC Cuts at: 0 148 322 2120 2403 Size: 148 174 1798 283 Fragments arranged by size: 1798 283 174 148 Bme1580I G_kGCm'C Cuts at: 0 639 1304 1730 1902 2403 Size: 639 665 426 172 501 Fragments arranged by size: 665 639 501 426 172 BmgBI CAC'GTC Cuts at: 0 513 567 2403 Size: 513 54 1836 Fragments arranged by size: 1836 513 54 BmrI ACTGGGnnnn_n' Cuts at: 0 525 1398 2403 Size: 525 873 1005 Fragments arranged by size: 1005 873 525 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 780 2403 Size: 780 1623 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 482 613 2022 2235 2403 Size: 244 238 131 1409 213 168 Fragments arranged by size: 1409 244 238 213 168 131 Bpu10I CC'TnA_GC Cuts at: 0 391 2403 Size: 391 2012 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 789 2403 Size: 789 1614 BsaI GGTCTCn'nnnn_ Cuts at: 0 1517 2403 Size: 1517 886 BsaAI yAC'GTr Cuts at: 0 1086 2403 Size: 1086 1317 BsaBI GATnn'nnATC Cuts at: 0 955 2403 Size: 955 1448 BsaHI Gr'CG_yC Cuts at: 0 1479 2068 2403 Size: 1479 589 335 Fragments arranged by size: 1479 589 335 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 26 1384 1414 1435 1465 2403 Size: 26 1358 30 21 30 938 Fragments arranged by size: 1358 938 30 30 26 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 310 2314 2403 Size: 310 2004 89 Fragments arranged by size: 2004 310 89 BseYI C'CCAG_C Cuts at: 0 1927 2403 Size: 1927 476 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2218 2403 Size: 2218 185 BsiEI CG_ry'CG Cuts at: 0 959 2403 Size: 959 1444 BsiHKAI G_wGCw'C Cuts at: 0 1022 1367 1868 1902 2403 Size: 1022 345 501 34 501 Fragments arranged by size: 1022 501 501 345 34 BsmI GAATG_Cn' Cuts at: 0 1888 2403 Size: 1888 515 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2059 2403 Size: 2059 344 Bsp1286I G_dGCh'C Cuts at: 0 50 639 775 1022 1304 1367 1562 1597 Size: 50 589 136 247 282 63 195 35 Cuts at: 1597 1730 1868 1902 2403 Size: 133 138 34 501 Fragments arranged by size: 589 501 282 247 195 138 136 133 63 50 35 34 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 288 1958 2403 Size: 288 1670 445 Fragments arranged by size: 1670 445 288 BsrBI CCG'CTC Cuts at: 0 2173 2403 Size: 2173 230 BsrDI GCAATG_nn' Cuts at: 0 1454 2403 Size: 1454 949 BsrFI r'CCGG_y Cuts at: 0 485 1475 2403 Size: 485 990 928 Fragments arranged by size: 990 928 485 BsrGI T'GTAC_A Cuts at: 0 545 2403 Size: 545 1858 BstBI TT'CG_AA Cuts at: 0 2262 2403 Size: 2262 141 BstYI r'GATC_y Cuts at: 0 257 1205 1533 2403 Size: 257 948 328 870 Fragments arranged by size: 948 870 328 257 Bsu36I CC'TnA_GG Cuts at: 0 134 327 2403 Size: 134 193 2076 Fragments arranged by size: 2076 193 134 BtgI C'CryG_G Cuts at: 0 425 693 2244 2403 Size: 425 268 1551 159 Fragments arranged by size: 1551 425 268 159 BtsI GCAGTG_nn' Cuts at: 0 836 1064 2403 Size: 836 228 1339 Fragments arranged by size: 1339 836 228 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 209 244 2076 2111 2403 Size: 209 35 1832 35 292 Fragments arranged by size: 1832 292 209 35 35 DraIII CAC_nnn'GTG Cuts at: 0 1086 2403 Size: 1086 1317 EaeI y'GGCC_r Cuts at: 0 428 971 1605 2403 Size: 428 543 634 798 Fragments arranged by size: 798 634 543 428 EarI CTCTTCn'nnn_ Cuts at: 0 496 745 2403 Size: 496 249 1658 Fragments arranged by size: 1658 496 249 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1462 1596 2403 Size: 1462 134 807 Fragments arranged by size: 1462 807 134 EcoICRI GAG'CTC Cuts at: 0 1020 1365 2403 Size: 1020 345 1038 Fragments arranged by size: 1038 1020 345 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 482 613 644 1275 2022 2235 2403 Size: 244 238 131 31 631 747 213 168 Fragments arranged by size: 747 631 244 238 213 168 131 31 EcoNI CCTnn'n_nnAGG Cuts at: 0 785 804 2403 Size: 785 19 1599 Fragments arranged by size: 1599 785 19 EcoO109I rG'GnC_Cy Cuts at: 0 80 650 2403 Size: 80 570 1753 Fragments arranged by size: 1753 570 80 EcoRI G'AATT_C Cuts at: 0 934 1220 2403 Size: 934 286 1183 Fragments arranged by size: 1183 934 286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 196 2403 Size: 196 2207 HaeII r_GCGC'y Cuts at: 0 822 1482 2403 Size: 822 660 921 Fragments arranged by size: 921 822 660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 923 955 1315 1347 1384 1416 2157 2189 Size: 923 32 360 32 37 32 741 32 Cuts at: 2189 2403 Size: 214 Fragments arranged by size: 923 741 360 214 37 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 178 2403 Size: 178 2225 KasI G'GCGC_C Cuts at: 0 1478 2403 Size: 1478 925 MfeI C'AATT_G Cuts at: 0 1617 2403 Size: 1617 786 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 146 2403 Size: 146 2257 MscI TGG'CCA Cuts at: 0 430 973 2403 Size: 430 543 1430 Fragments arranged by size: 1430 543 430 MslI CAynn'nnrTG Cuts at: 0 424 553 557 692 2290 2403 Size: 424 129 4 135 1598 113 Fragments arranged by size: 1598 424 135 129 113 4 MspA1I CmG'CkG Cuts at: 0 319 1388 1494 2307 2313 2403 Size: 319 1069 106 813 6 90 Fragments arranged by size: 1069 813 319 106 90 6 NaeI GCC'GGC Cuts at: 0 1477 2403 Size: 1477 926 NarI GG'CG_CC Cuts at: 0 1479 2403 Size: 1479 924 NcoI C'CATG_G Cuts at: 0 425 2244 2403 Size: 425 1819 159 Fragments arranged by size: 1819 425 159 NgoMIV G'CCGG_C Cuts at: 0 1475 2403 Size: 1475 928 NspI r_CATG'y Cuts at: 0 546 669 2130 2403 Size: 546 123 1461 273 Fragments arranged by size: 1461 546 273 123 PciI A'CATG_T Cuts at: 0 542 2126 2403 Size: 542 1584 277 Fragments arranged by size: 1584 542 277 PflMI CCAn_nnn'nTGG Cuts at: 0 1086 2403 Size: 1086 1317 PmlI CAC'GTG Cuts at: 0 1086 2403 Size: 1086 1317 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1435 1467 2403 Size: 1435 32 936 Fragments arranged by size: 1435 936 32 PpuMI rG'GwC_Cy Cuts at: 0 80 2403 Size: 80 2323 PstI C_TGCA'G Cuts at: 0 1545 1703 2312 2403 Size: 1545 158 609 91 Fragments arranged by size: 1545 609 158 91 PvuI CG_AT'CG Cuts at: 0 959 2403 Size: 959 1444 PvuII CAG'CTG Cuts at: 0 1388 1494 2307 2313 2403 Size: 1388 106 813 6 90 Fragments arranged by size: 1388 813 106 90 6 SacI G_AGCT'C Cuts at: 0 1022 1367 2403 Size: 1022 345 1036 Fragments arranged by size: 1036 1022 345 SanDI GG'GwC_CC Cuts at: 0 80 2403 Size: 80 2323 SapI GCTCTTCn'nnn_ Cuts at: 0 496 2403 Size: 496 1907 SfcI C'TryA_G Cuts at: 0 1541 1699 1858 2308 2403 Size: 1541 158 159 450 95 Fragments arranged by size: 1541 450 159 158 95 SfiI GGCCn_nnn'nGGCC Cuts at: 0 148 322 2403 Size: 148 174 2081 Fragments arranged by size: 2081 174 148 SfoI GGC'GCC Cuts at: 0 1480 2403 Size: 1480 923 SgrAI Cr'CCGG_yG Cuts at: 0 1475 2403 Size: 1475 928 SmlI C'TyrA_G Cuts at: 0 768 2403 Size: 768 1635 StuI AGG'CCT Cuts at: 0 151 1327 2403 Size: 151 1176 1076 Fragments arranged by size: 1176 1076 151 StyI C'CwwG_G Cuts at: 0 425 452 1127 2244 2403 Size: 425 27 675 1117 159 Fragments arranged by size: 1117 675 425 159 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 559 2403 Size: 559 1844 TatI w'GTAC_w Cuts at: 0 465 545 2403 Size: 465 80 1858 Fragments arranged by size: 1858 465 80 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2020 2094 2403 Size: 2020 74 309 Fragments arranged by size: 2020 309 74 ZraI GAC'GTC Cuts at: 0 2069 2403 Size: 2069 334 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmlI PpiI PpuMI PstI PvuI PvuII SacI SanDI SapI SfcI SfiI SfoI SgrAI SmlI StuI StyI TaqII TatI XcmI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AhdI ApaI AscI AseI AsiSI AvrII BcgI BciVI BclI BfrBI BglII BlpI BmtI BsaWI BsiWI BspEI BspHI BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgZI ClaI DraI DrdI EagI EcoRV FseI FspI FspAI HincII HpaI KpnI MluI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmeI PshAI PsiI PspOMI PspXI PsrI RsrII SacII SalI SbfI ScaI SexAI SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI