(Circular) (Six-base) MAPSORT of: pFN21AB5675.seq Check: 183 from: 1 to: 7738 pFN21AB5675 7738 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2211 2704 3223 4716 2211 Size: 493 519 1493 5233 Fragments arranged by size: 5233 1493 519 493 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5548 278 Size: 53 83 186 936 4012 2468 Fragments arranged by size: 4012 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5081 1937 Size: 3144 4594 Acc65I G'GTAC_C Cuts at: 5059 5059 Size: 7738 AclI AA'CG_TT Cuts at: 1563 3848 5865 6238 1563 Size: 2285 2017 373 3063 Fragments arranged by size: 3063 2285 2017 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2759 3712 3973 4478 5801 7185 7453 1632 Size: 1127 953 261 505 1323 1384 268 1917 Fragments arranged by size: 1917 1384 1323 1127 953 505 268 261 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7541 AflIII A'CryG_T Cuts at: 1203 2982 6637 7614 1203 Size: 1779 3655 977 1327 Fragments arranged by size: 3655 1779 1327 977 AgeI A'CCGG_T Cuts at: 3170 6569 3170 Size: 3399 4339 AhdI GACnn_n'nnGTC Cuts at: 6467 6467 Size: 7738 AleI CACnn'nnGTG Cuts at: 1166 2289 2499 2768 4871 1166 Size: 1123 210 269 2103 4033 Fragments arranged by size: 4033 2103 1123 269 210 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2418 3653 4706 4782 1184 Size: 1234 1235 1053 76 4140 Fragments arranged by size: 4140 1235 1234 1053 76 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3683 3715 3683 Size: 32 7706 AlwNI CAG_nnn'CTG Cuts at: 2701 2836 3504 5481 7053 2701 Size: 135 668 1977 1572 3386 Fragments arranged by size: 3386 1977 1572 668 135 ApaI G_GGCC'C Cuts at: 4733 4733 Size: 7738 ApaLI G'TGCA_C Cuts at: 2198 3303 4070 4442 4703 5795 6951 2198 Size: 1105 767 372 261 1092 1156 2985 Fragments arranged by size: 2985 1156 1105 1092 767 372 261 ApoI r'AATT_y Cuts at: 1493 5047 5273 5425 7534 1493 Size: 3554 226 152 2109 1697 Fragments arranged by size: 3554 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6292 160 Size: 872 5260 1606 Fragments arranged by size: 5260 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7738 AvaI C'yCGr_G Cuts at: 1870 1943 2145 2607 2858 4621 4646 5063 1870 Size: 73 202 462 251 1763 25 417 4545 Fragments arranged by size: 4545 1763 462 417 251 202 73 25 BamHI G'GATC_C Cuts at: 5068 5435 5068 Size: 367 7371 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2388 2442 2501 2661 Size: 325 217 627 222 379 54 59 160 Cuts at: 2661 3356 3751 3977 4128 4524 4631 5059 6514 Size: 695 395 226 151 396 107 428 1455 Cuts at: 6514 618 Size: 1842 Fragments arranged by size: 1842 1455 695 627 428 396 395 379 325 226 222 217 160 151 107 59 54 BanII G_rGCy'C Cuts at: 1391 2957 4033 4314 4437 4654 4733 5057 1391 Size: 1566 1076 281 123 217 79 324 4072 Fragments arranged by size: 4072 1566 1076 324 281 217 123 79 BbeI G_GCGC'C Cuts at: 2013 2446 2665 4528 4635 2013 Size: 433 219 1863 107 5116 Fragments arranged by size: 5116 1863 433 219 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4055 928 Size: 971 2156 4611 Fragments arranged by size: 4611 2156 971 BbvCI CC'TCA_GC Cuts at: 3215 3215 Size: 7738 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3310 3344 5929 5963 1903 Size: 34 1373 34 2585 34 3678 Fragments arranged by size: 3678 2585 1373 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2150 5630 6840 2150 Size: 3480 1210 3048 Fragments arranged by size: 3480 3048 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7738 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2330 6349 136 Size: 107 122 71 631 605 658 4019 1525 Fragments arranged by size: 4019 1525 658 631 605 122 107 71 BglII A'GATC_T Cuts at: 7733 7733 Size: 7738 BlpI GC'TnA_GC Cuts at: 3512 5155 3512 Size: 1643 6095 Bme1580I G_kGCm'C Cuts at: 1790 2202 3307 3642 4074 4446 4707 4733 5799 Size: 412 1105 335 432 372 261 26 1066 Cuts at: 5799 6955 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1105 1066 432 412 372 335 261 26 BmgBI CAC'GTC Cuts at: 1318 2227 1318 Size: 909 6829 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5469 6427 442 Size: 416 53 83 399 24 4052 958 1753 Fragments arranged by size: 4052 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7738 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2635 4915 2635 Size: 2280 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2468 2561 2723 3269 3317 4154 4235 4283 Size: 1229 93 162 546 48 837 81 48 Cuts at: 4283 4451 4885 6398 1239 Size: 168 434 1513 2579 Fragments arranged by size: 2579 1513 1229 837 546 434 168 162 93 81 48 48 Bpu10I CC'TnA_GC Cuts at: 3215 4796 3215 Size: 1581 6157 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4840 4892 5863 6728 7026 4840 Size: 52 971 865 298 5552 Fragments arranged by size: 5552 971 865 298 52 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2418 4856 6401 882 Size: 617 919 2438 1545 2219 Fragments arranged by size: 2438 2219 1545 919 617 BsaAI yAC'GTr Cuts at: 493 1204 2359 3373 5529 493 Size: 711 1155 1014 2156 2702 Fragments arranged by size: 2702 2156 1155 1014 711 BsaBI GATnn'nnATC Cuts at: 4352 7732 4352 Size: 3380 4358 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2443 2662 3345 Size: 53 83 186 936 477 433 219 683 Cuts at: 3345 4525 4632 5545 5927 6562 275 Size: 1180 107 913 382 635 1451 Fragments arranged by size: 1451 1180 936 913 683 635 477 433 382 219 186 107 83 53 BsaWI w'CCGG_w Cuts at: 3170 3293 3688 4784 6171 6569 6843 6990 7401 Size: 123 395 1096 1387 398 274 147 411 Cuts at: 7401 3170 Size: 3507 Fragments arranged by size: 3507 1387 1096 411 398 395 274 147 123 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2702 2732 3683 3713 4127 Size: 30 165 30 1299 30 951 30 414 Cuts at: 4127 4157 4487 4517 4560 4590 4748 4778 4817 Size: 30 330 30 43 30 158 30 39 Cuts at: 4817 4847 1178 Size: 30 4069 Fragments arranged by size: 4069 1299 951 414 330 165 158 43 39 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4282 4644 1186 Size: 3096 362 4280 Fragments arranged by size: 4280 3096 362 BseYI C'CCAG_C Cuts at: 2466 2808 3408 3641 3662 4026 4725 6941 7437 Size: 342 600 233 21 364 699 2216 496 Cuts at: 7437 2466 Size: 2767 Fragments arranged by size: 2767 2216 699 600 496 364 342 233 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2315 4082 1163 Size: 573 462 117 1767 4819 Fragments arranged by size: 4819 1767 573 462 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2751 3191 5188 5949 6977 1540 Size: 275 186 750 440 1997 761 1028 2301 Fragments arranged by size: 2301 1997 1028 761 750 440 275 186 BsiHKAI G_wGCw'C Cuts at: 2146 2202 3307 3333 3837 4074 4437 4446 4707 Size: 56 1105 26 504 237 363 9 261 Cuts at: 4707 4761 5799 5884 6955 2146 Size: 54 1038 85 1071 2929 Fragments arranged by size: 2929 1105 1071 1038 504 363 261 237 85 56 54 26 9 BsmI GAATG_Cn' Cuts at: 2980 5249 5342 2980 Size: 2269 93 5376 Fragments arranged by size: 5376 2269 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2202 2957 3307 3333 3642 3837 Size: 399 356 56 755 350 26 309 195 Cuts at: 3837 4033 4074 4314 4437 4446 4654 4707 4733 Size: 196 41 240 123 9 208 53 26 Cuts at: 4733 4761 5057 5799 5884 6955 1391 Size: 28 296 742 85 1071 2174 Fragments arranged by size: 2174 1071 755 742 399 356 350 309 296 240 208 196 195 123 85 56 53 41 28 26 26 9 BspEI T'CCGG_A Cuts at: 3293 3688 4784 7401 3293 Size: 395 1096 2617 3630 Fragments arranged by size: 3630 2617 1096 395 BspHI T'CATG_A Cuts at: 5627 7357 5627 Size: 1730 6008 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2211 2704 3223 4698 4716 5093 844 Size: 1367 493 519 1475 18 377 3489 Fragments arranged by size: 3489 1475 1367 519 493 377 18 BsrBI CCG'CTC Cuts at: 1394 1823 2098 3511 4395 5625 1394 Size: 429 275 1413 884 1230 3507 Fragments arranged by size: 3507 1413 1230 884 429 275 BsrDI GCAATG_nn' Cuts at: 65 1248 2563 4252 6233 6407 65 Size: 1183 1315 1689 1981 174 1396 Fragments arranged by size: 1981 1689 1396 1315 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2138 2288 2916 3170 3190 3589 4572 Size: 110 328 150 628 254 20 399 983 Cuts at: 4572 6382 6569 1700 Size: 1810 187 2869 Fragments arranged by size: 2869 1810 983 628 399 328 254 187 150 110 20 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7738 BssHII G'CGCG_C Cuts at: 1925 3389 3527 1925 Size: 1464 138 6136 Fragments arranged by size: 6136 1464 138 BssSI C'ACGA_G Cuts at: 5798 6810 5798 Size: 1012 6726 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 3904 4069 794 Size: 1412 1698 165 4463 Fragments arranged by size: 4463 1698 1412 165 BstBI TT'CG_AA Cuts at: 6542 6542 Size: 7738 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7738 BstXI CCAn_nnnn'nTGG Cuts at: 2878 3649 4873 2878 Size: 771 1224 5743 Fragments arranged by size: 5743 1224 771 BstYI r'GATC_y Cuts at: 1972 2335 3800 4035 4257 5068 5435 5820 5837 Size: 363 1465 235 222 811 367 385 17 Cuts at: 5837 7289 7375 7387 7733 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1465 1452 811 385 367 363 346 235 222 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3973 4605 3973 Size: 632 7106 BtgI C'CryG_G Cuts at: 513 1065 2004 2244 2751 2949 4314 4986 513 Size: 552 939 240 507 198 1365 672 3265 Fragments arranged by size: 3265 1365 939 672 552 507 240 198 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3335 3660 487 Size: 683 56 2109 325 4565 Fragments arranged by size: 4565 2109 683 325 56 BtsI GCAGTG_nn' Cuts at: 2904 3052 3710 5254 6048 6068 2904 Size: 148 658 1544 794 20 4574 Fragments arranged by size: 4574 1544 794 658 148 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7738 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2663 2698 529 Size: 35 2099 35 5569 Fragments arranged by size: 5569 2099 35 35 DraI TTT'AAA Cuts at: 5042 5042 Size: 7738 DraIII CAC_nnn'GTG Cuts at: 2941 4045 4534 2941 Size: 1104 489 6145 Fragments arranged by size: 6145 1104 489 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6745 809 Size: 507 5429 1802 Fragments arranged by size: 5429 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2090 2599 2901 Size: 54 1425 50 275 18 260 509 302 Cuts at: 2901 3188 3270 3375 4249 4719 4875 4965 5185 Size: 287 82 105 874 470 156 90 220 Cuts at: 5185 6074 7703 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 874 509 470 302 287 275 260 220 156 105 90 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3188 5185 1537 Size: 275 1376 1997 4090 Fragments arranged by size: 4090 1997 1376 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2765 5668 1353 Size: 1412 2903 3423 Fragments arranged by size: 3423 2903 1412 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3577 4598 6325 6699 6845 387 Size: 1064 138 1988 1021 1727 374 146 1280 Fragments arranged by size: 1988 1727 1280 1064 1021 374 146 138 EcoICRI GAG'CTC Cuts at: 4435 4435 Size: 7738 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2468 2561 2723 2759 3269 3317 3712 Size: 393 836 93 162 36 510 48 395 Cuts at: 3712 3973 4154 4235 4283 4451 4478 4885 5801 Size: 261 181 81 48 168 27 407 916 Cuts at: 5801 6398 7185 7453 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 916 836 787 597 510 407 395 393 268 261 181 168 162 93 81 48 48 36 27 EcoNI CCTnn'n_nnAGG Cuts at: 3314 3861 3314 Size: 547 7191 EcoO109I rG'GnC_Cy Cuts at: 2063 2845 3281 3413 3619 4594 4687 4729 4730 Size: 782 436 132 206 975 93 42 1 Cuts at: 4730 4825 4928 2063 Size: 95 103 4873 Fragments arranged by size: 4873 975 782 436 206 132 103 95 93 42 1 EcoRI G'AATT_C Cuts at: 5047 5425 5047 Size: 378 7360 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4072 4246 4072 Size: 174 7564 FseI GG_CCGG'CC Cuts at: 3194 3194 Size: 7738 FspI TGC'GCA Cuts at: 2631 3027 4682 6244 2631 Size: 396 1655 1562 4125 Fragments arranged by size: 4125 1655 1562 396 HaeII r_GCGC'y Cuts at: 1678 2013 2446 2665 3214 3257 4528 4635 4803 Size: 335 433 219 549 43 1271 107 168 Cuts at: 4803 6885 1678 Size: 2082 2531 Fragments arranged by size: 2531 2082 1271 549 433 335 219 168 107 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3956 3988 4487 4519 4560 Size: 32 135 32 2384 32 499 32 41 Cuts at: 4560 4592 4817 4849 6379 6411 6453 6485 1373 Size: 32 225 32 1530 32 42 32 2626 Fragments arranged by size: 2626 2384 1530 499 225 135 42 41 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4339 5082 5328 669 Size: 988 281 2401 743 246 3079 Fragments arranged by size: 3079 2401 988 743 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7738 HpaI GTT'AAC Cuts at: 5328 5328 Size: 7738 KasI G'GCGC_C Cuts at: 2009 2442 2661 4524 4631 2009 Size: 433 219 1863 107 5116 Fragments arranged by size: 5116 1863 433 219 107 KpnI G_GTAC'C Cuts at: 5063 5063 Size: 7738 MfeI C'AATT_G Cuts at: 5337 5337 Size: 7738 MluI A'CGCG_T Cuts at: 7614 7614 Size: 7738 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6852 7036 1305 Size: 5547 184 2007 Fragments arranged by size: 5547 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2903 3377 4721 4877 10 Size: 54 1425 343 1071 474 1344 156 2871 Fragments arranged by size: 2871 1425 1344 1071 474 343 156 54 MslI CAynn'nnrTG Cuts at: 518 1166 2217 2289 2357 2499 2768 2942 4871 Size: 648 1051 72 68 142 269 174 1929 Cuts at: 4871 5696 6055 6214 518 Size: 825 359 159 2042 Fragments arranged by size: 2042 1929 1051 825 648 359 269 174 159 142 72 68 MspA1I CmG'CkG Cuts at: 1606 2753 2836 3016 3247 3354 4012 4026 4126 Size: 1147 83 180 231 107 658 14 100 Cuts at: 4126 4213 4375 4725 4957 5154 5831 6979 7224 Size: 87 162 350 232 197 677 1148 245 Cuts at: 7224 1606 Size: 2120 Fragments arranged by size: 2120 1148 1147 677 658 350 245 232 231 197 180 162 107 100 87 83 14 NaeI GCC'GGC Cuts at: 3192 3192 Size: 7738 NarI GG'CG_CC Cuts at: 2010 2443 2662 4525 4632 2010 Size: 433 219 1863 107 5116 Fragments arranged by size: 5116 1863 433 219 107 NcoI C'CATG_G Cuts at: 513 1065 2004 2949 4986 513 Size: 552 939 945 2037 3265 Fragments arranged by size: 3265 2037 945 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7738 NgoMIV G'CCGG_C Cuts at: 3190 3190 Size: 7738 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7738 NotI GC'GGCC_GC Cuts at: 5185 5185 Size: 7738 NspI r_CATG'y Cuts at: 1132 1229 2169 2191 2986 4372 5096 6641 1132 Size: 97 940 22 795 1386 724 1545 2229 Fragments arranged by size: 2229 1545 1386 940 795 724 97 22 PasI CC'CwG_GG Cuts at: 1348 2911 3556 3721 4399 1348 Size: 1563 645 165 678 4687 Fragments arranged by size: 4687 1563 678 645 165 PciI A'CATG_T Cuts at: 2982 6637 2982 Size: 3655 4083 PflMI CCAn_nnn'nTGG Cuts at: 1108 2985 3373 1108 Size: 1877 388 5473 Fragments arranged by size: 5473 1877 388 PfoI T'CCnGG_A Cuts at: 2304 4260 2304 Size: 1956 5782 PmeI GTTT'AAAC Cuts at: 5042 5042 Size: 7738 PmlI CAC'GTG Cuts at: 3373 3373 Size: 7738 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5807 5839 7320 7352 3683 Size: 32 2092 32 1481 32 4069 Fragments arranged by size: 4069 2092 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2845 3281 3619 4594 4687 4825 2845 Size: 436 338 975 93 138 5758 Fragments arranged by size: 5758 975 436 338 138 93 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7738 PsiI TTA'TAA Cuts at: 3549 5308 3549 Size: 1759 5979 PspOMI G'GGCC_C Cuts at: 4729 4729 Size: 7738 PstI C_TGCA'G Cuts at: 830 3234 4561 4860 5090 830 Size: 2404 1327 299 230 3478 Fragments arranged by size: 3478 2404 1327 299 230 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7738 PvuII CAG'CTG Cuts at: 3016 3247 4375 4725 4957 3016 Size: 231 1128 350 232 5797 Fragments arranged by size: 5797 1128 350 232 231 SacI G_AGCT'C Cuts at: 4437 4437 Size: 7738 SacII CC_GC'GG Cuts at: 2754 2754 Size: 7738 SalI G'TCGA_C Cuts at: 1936 5080 1936 Size: 3144 4594 SanDI GG'GwC_CC Cuts at: 3281 3281 Size: 7738 SbfI CC_TGCA'GG Cuts at: 3234 4860 5090 3234 Size: 1626 230 5882 Fragments arranged by size: 5882 1626 230 ScaI AGT'ACT Cuts at: 2255 5986 7698 2255 Size: 3731 1712 2295 Fragments arranged by size: 3731 2295 1712 SexAI A'CCwGG_T Cuts at: 3110 4041 4530 3110 Size: 931 489 6318 Fragments arranged by size: 6318 931 489 SfcI C'TryA_G Cuts at: 826 1045 3230 4557 4856 5086 6221 6902 7093 Size: 219 2185 1327 299 230 1135 681 191 Cuts at: 7093 826 Size: 1471 Fragments arranged by size: 2185 1471 1327 1135 681 299 230 219 191 SfoI GGC'GCC Cuts at: 2011 2444 2663 4526 4633 2011 Size: 433 219 1863 107 5116 Fragments arranged by size: 5116 1863 433 219 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7738 SgrAI Cr'CCGG_yG Cuts at: 1700 2288 1700 Size: 588 7150 SmaI CCC'GGG Cuts at: 1872 4623 5065 1872 Size: 2751 442 4545 Fragments arranged by size: 4545 2751 442 SmlI C'TyrA_G Cuts at: 820 1017 1943 4819 4907 5842 6743 7005 820 Size: 197 926 2876 88 935 901 262 1553 Fragments arranged by size: 2876 1553 935 926 901 262 197 88 SnaBI TAC'GTA Cuts at: 493 5529 493 Size: 5036 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7738 SphI G_CATG'C Cuts at: 1132 5096 1132 Size: 3964 3774 SspI AAT'ATT Cuts at: 5 52 3657 5662 5 Size: 47 3605 2005 2081 Fragments arranged by size: 3605 2081 2005 47 StuI AGG'CCT Cuts at: 1517 2618 3971 1517 Size: 1101 1353 5284 Fragments arranged by size: 5284 1353 1101 StyI C'CwwG_G Cuts at: 513 1065 2004 2795 2871 2949 4056 4986 5177 Size: 552 939 791 76 78 1107 930 191 Cuts at: 5177 513 Size: 3074 Fragments arranged by size: 3074 1107 939 930 791 552 191 78 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2302 2458 2507 4504 5765 5782 5935 Size: 1255 722 156 49 1997 1261 17 153 Cuts at: 5935 6120 6565 325 Size: 185 445 1498 Fragments arranged by size: 1997 1498 1261 1255 722 445 185 156 153 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 2298 2862 Size: 274 80 33 51 1443 276 45 564 Cuts at: 2862 5984 7696 96 Size: 3122 1712 138 Fragments arranged by size: 3122 1712 1443 564 276 274 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2702 2734 2804 2836 3774 3806 4127 4159 7698 Size: 32 70 32 938 32 321 32 3539 Cuts at: 7698 7730 2702 Size: 32 2710 Fragments arranged by size: 3539 2710 938 321 70 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2074 2312 3347 1316 Size: 758 238 1035 5707 Fragments arranged by size: 5707 1035 758 238 XbaI T'CTAG_A Cuts at: 5074 5074 Size: 7738 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2258 2522 4975 2258 Size: 264 2453 5021 Fragments arranged by size: 5021 2453 264 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7738 XmaI C'CCGG_G Cuts at: 1870 4621 5063 1870 Size: 2751 442 4545 Fragments arranged by size: 4545 2751 442 XmnI GAAnn'nnTTC Cuts at: 5867 5867 Size: 7738 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5546 276 Size: 53 83 186 936 4012 2468 Fragments arranged by size: 4012 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SfiI SrfI SwaI