Restriction map for
pFN21AE0208
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MroI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 910 GTCTAC GT^[AC][GT]AC
AflIII 233 ACGCGT A^C[GA][CT]GT
ApaI 313 GGGCCC GGGCC^C
Asp700I 818 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 370 GTGCTC G[AT]GC[AT]^C
AviII 278 TGCGCA TGC^GCA
BalI 290 TGGCCA TGG^CCA
BanI 402 GGTGCC G^G[CT][GA]CC
BanII 313 GGGCCC G[GA]GC[CT]^C
BbsI 653 GTCTTC GAAGAC(2/6)
BfaI 1073 CTAG C^TAG
BpuAI 653 GTCTTC GAAGAC(2/6)
BsiEI 29 CGGCCG CG[GA][CT]^CG
BsiHKAI 370 GTGCTC G[AT]GC[AT]^C
BsmAI 76 GAGAC GTCTC(1/5)
BsmBI 76 GAGACG CGTCTC(1/5)
BsmFI 54 GTCCC GGGAC(10/14)
BsmI 1091 GAATGC GAATGC(1/-1)
BsrFI 302 ACCGGC [GA]^CCGG[CT]
BstBI 815 TTCGAA TT^CGAA
BstF5I 471 CATCC GGATG(2/0)
BstXI 329 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 689 AGATCC [GA]^GATC[CT]
Cfr10I 302 ACCGGC [GA]^CCGG[CT]
DraII 332 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 178 CCACGG C^C[GA][CT]GG
EagI 29 CGGCCG C^GGCCG
EarI 712 GAAGAG CTCTTC(1/4)
EclXI 29 CGGCCG C^GGCCG
Eco52I 29 CGGCCG C^GGCCG
EcoO109I 332 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 329 CCAAGG C^C[AT][AT]GG
FokI 471 CATCC GGATG(9/13)
FspI 278 TGCGCA TGC^GCA
HgaI 78 GACGC GACGC(5/10)
HincII 237 GTTGAC GT[CT]^[GA]AC
HindII 237 GTTGAC GT[CT]^[GA]AC
HphI 994 TCACC GGTGA(8/7)
Ksp632I 712 GAAGAG CTCTTC(1/4)
MaeI 1073 CTAG C^TAG
MaeII 221 ACGT A^CGT
MfeI 882 CAATTG C^AATTG
MflI 689 AGATCC [GA]^GATC[CT]
MluI 233 ACGCGT A^CGCGT
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MslI 995 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 882 CAATTG C^AATTG
NspV 815 TTCGAA TT^CGAA
PaeR7I 683 CTCGAG C^TCGAG
PvuII 211 CAGCTG CAG^CTG
SfiI 30 GGCCGGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 815 TTCGAA TT^CGAA
SmlI 683 CTCGAG C^T[CT][GA]AG
SspI 820 AATATT AAT^ATT
StyI 329 CCAAGG C^C[AT][AT]GG
XcmI 71 CCACCGAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 683 CTCGAG C^TCGAG
XhoII 689 AGATCC [GA]^GATC[CT]
XmnI 818 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 525 AAATTT [GA]^AATT[CT]
940 AAATTT
AlwI 690 GATCC GGATC(4/5)
772 GATCC
AlwNI 211 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
436 CAGACCCTG
ApoI 525 AAATTT [GA]^AATT[CT]
940 AAATTT
AvaI 247 CTCGGG C^[CT]CG[GA]G
683 CTCGAG
AvaII 53 GGTCC G^G[AT]CC
803 GGACC
BbvCI 60 CCTCAGC CCTCAGC(-5/-2)
378 CCTCAGC
BcnI 32 CCGGG CC^[GC]GG
474 CCCGG
BglI 31 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
446 GCCTGGGCGGC
BmyI 313 GGGCCC G[AGT]GC[ACT]^C
370 GTGCTC
BsgI 797 CTGCAC GTGCAG(16/14)
1082 CTGCAC
BsoBI 247 CTCGGG C^[CT]CG[GA]G
683 CTCGAG
Bsp1286I 313 GGGCCC G[AGT]GC[ACT]^C
370 GTGCTC
BssHII 426 GCGCGC G^CGCGC
428 GCGCGC
DrdI 78 GACGCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
919 GACTGCTGTGTC
EaeI 29 CGGCCG [CT]^GGCC[GA]
290 TGGCCA
HaeII 132 GGCGCT [GA]GCGC^[CT]
183 GGCGCT
NciI 32 CCGGG CC^[GC]GG
474 CCCGG
PflMI 210 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
895 CCAAAGTGTGG
PstI 433 CTGCAG CTGCA^G
463 CTGCAG
SfaNI 978 GATGC GCATC(5/9)
470 GCATC
SfcI 433 CTGCAG C^T[GA][CT]AG
463 CTGCAG
TfiI 813 GATTC G^A[AT]TC
1051 GAATC
Tsp45I 25 GTCAC ^GT[GC]AC
590 GTGAC
TspRI 318 CAGTG CA[GC]TG[ACGT][ACGT]^
974 CACTG
Van91I 210 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
895 CCAAAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI BbvI BpmI
BsaJI BseRI BsiYI BslI BsrBI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIV PleI Sau3AI Sau96I ScrFI
TaqI Tru9I TseI Tsp509I TthHB8I