Restriction map for
pFN21AE0208
(Linear) (Six-base) MAPSORT of: KIEE0208.seq Check: 7653 from: 1 to: 1113
KIEE0208.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 0 911 1113
Size: 911 202
AflIII A'CryG_T
Cuts at: 0 233 1113
Size: 233 880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 1113
Size: 478 635
AlwNI CAG_nnn'CTG
Cuts at: 0 216 441 1113
Size: 216 225 672
Fragments arranged by size:
672 225 216
ApaI G_GGCC'C
Cuts at: 0 317 1113
Size: 317 796
ApoI r'AATT_y
Cuts at: 0 525 940 1113
Size: 525 415 173
Fragments arranged by size:
525 415 173
AvaI C'yCGr_G
Cuts at: 0 247 683 1113
Size: 247 436 430
Fragments arranged by size:
436 430 247
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 855 888 1113
Size: 855 33 225
Fragments arranged by size:
855 225 33
BanI G'GyrC_C
Cuts at: 0 402 1113
Size: 402 711
BanII G_rGCy'C
Cuts at: 0 317 1113
Size: 317 796
BbsI GAAGACnn'nnnn_
Cuts at: 0 646 1113
Size: 646 467
BbvCI CC'TCA_GC
Cuts at: 0 61 379 1113
Size: 61 318 734
Fragments arranged by size:
734 318 61
BglI GCCn_nnn'nGGC
Cuts at: 0 37 452 1113
Size: 37 415 661
Fragments arranged by size:
661 415 37
Bme1580I G_kGCm'C
Cuts at: 0 317 1113
Size: 317 796
BmgBI CAC'GTC
Cuts at: 0 222 1113
Size: 222 891
BmrI ACTGGGnnnn_n'
Cuts at: 0 311 336 1113
Size: 311 25 777
Fragments arranged by size:
777 311 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 709 1113
Size: 709 404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 115 193 349 1113
Size: 103 12 78 156 764
Fragments arranged by size:
764 156 103 78 12
Bpu10I CC'TnA_GC
Cuts at: 0 61 379 442 1113
Size: 61 318 63 671
Fragments arranged by size:
671 318 63 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 35 1113
Size: 5 30 1078
Fragments arranged by size:
1078 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 124 605 920 1113
Size: 19 105 481 315 193
Fragments arranged by size:
481 315 193 105 19
BseYI C'CCAG_C
Cuts at: 0 186 1113
Size: 186 927
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1067 1113
Size: 782 285 46
Fragments arranged by size:
782 285 46
BsiEI CG_ry'CG
Cuts at: 0 32 1113
Size: 32 1081
BsiHKAI G_wGCw'C
Cuts at: 0 374 1113
Size: 374 739
BsmI GAATG_Cn'
Cuts at: 0 1097 1113
Size: 1097 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 70 1113
Size: 70 1043
Bsp1286I G_dGCh'C
Cuts at: 0 317 374 1113
Size: 317 57 739
Fragments arranged by size:
739 317 57
BsrBI CCG'CTC
Cuts at: 0 114 227 351 1113
Size: 114 113 124 762
Fragments arranged by size:
762 124 114 113
BsrFI r'CCGG_y
Cuts at: 0 302 1113
Size: 302 811
BssHII G'CGCG_C
Cuts at: 0 426 428 1113
Size: 426 2 685
Fragments arranged by size:
685 426 2
BstBI TT'CG_AA
Cuts at: 0 816 1113
Size: 816 297
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 1113
Size: 336 777
BstYI r'GATC_y
Cuts at: 0 689 1113
Size: 689 424
BtgI C'CryG_G
Cuts at: 0 178 1113
Size: 178 935
DrdI GACnn_nn'nnGTC
Cuts at: 0 84 925 1113
Size: 84 841 188
Fragments arranged by size:
841 188 84
EaeI y'GGCC_r
Cuts at: 0 29 290 1113
Size: 29 261 823
Fragments arranged by size:
823 261 29
EagI C'GGCC_G
Cuts at: 0 29 1113
Size: 29 1084
EarI CTCTTCn'nnn_
Cuts at: 0 707 1113
Size: 707 406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 115 193 349 1113
Size: 103 12 78 156 764
Fragments arranged by size:
764 156 103 78 12
EcoO109I rG'GnC_Cy
Cuts at: 0 333 1113
Size: 333 780
FspI TGC'GCA
Cuts at: 0 280 1113
Size: 280 833
HaeII r_GCGC'y
Cuts at: 0 136 187 1113
Size: 136 51 926
Fragments arranged by size:
926 136 51
HincII GTy'rAC
Cuts at: 0 239 1113
Size: 239 874
MfeI C'AATT_G
Cuts at: 0 882 1113
Size: 882 231
MluI A'CGCG_T
Cuts at: 0 233 1113
Size: 233 880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 1113
Size: 802 311
MscI TGG'CCA
Cuts at: 0 292 1113
Size: 292 821
MslI CAynn'nnrTG
Cuts at: 0 999 1113
Size: 999 114
MspA1I CmG'CkG
Cuts at: 0 19 194 213 383 1113
Size: 19 175 19 170 730
Fragments arranged by size:
730 175 170 19 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 216 901 1113
Size: 216 685 212
Fragments arranged by size:
685 216 212
PfoI T'CCnGG_A
Cuts at: 0 395 785 846 1113
Size: 395 390 61 267
Fragments arranged by size:
395 390 267 61
PspOMI G'GGCC_C
Cuts at: 0 313 1113
Size: 313 800
PstI C_TGCA'G
Cuts at: 0 437 467 1113
Size: 437 30 646
Fragments arranged by size:
646 437 30
PvuII CAG'CTG
Cuts at: 0 213 1113
Size: 213 900
SfcI C'TryA_G
Cuts at: 0 433 463 1113
Size: 433 30 650
Fragments arranged by size:
650 433 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 37 1113
Size: 37 1076
SmlI C'TyrA_G
Cuts at: 0 683 1113
Size: 683 430
SspI AAT'ATT
Cuts at: 0 822 1113
Size: 822 291
StyI C'CwwG_G
Cuts at: 0 329 1113
Size: 329 784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5 37 1113
Size: 5 32 1076
Fragments arranged by size:
1076 32 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 78 1113
Size: 78 1035
XhoI C'TCGA_G
Cuts at: 0 683 1113
Size: 683 430
XmnI GAAnn'nnTTC
Cuts at: 0 822 1113
Size: 822 291
Enzymes that do cut:
AccI AflIII AlfI AlwNI ApaI ApoI AvaI BaeI
BanI BanII BbsI BbvCI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrFI BssHII BstBI
BstXI BstYI BtgI DrdI EaeI EagI EarI Eco57MI
EcoO109I FspI HaeII HincII MfeI MluI MmeI MscI
MslI MspA1I PflMI PfoI PspOMI PstI PvuII SfcI
SfiI SmlI SspI StyI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiWI
BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HindIII HpaI KasI KpnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TatI
Tth111I XbaI XmaI ZraI