(Circular) (Six-base) MAPSORT of: pFN21AB6492.seq Check: 8025 from: 1 to: 5776 pFN21AB6492 5776 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2389 2389 Size: 5776 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2696 3839 5223 5491 1632 Size: 641 423 1143 1384 268 1917 Fragments arranged by size: 1917 1384 1143 641 423 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2669 1184 Size: 1485 4291 AlwNI CAG_nnn'CTG Cuts at: 2209 2707 3037 3519 5091 2209 Size: 498 330 482 1572 2894 Fragments arranged by size: 2894 1572 498 482 330 ApaI G_GGCC'C Cuts at: 2079 2079 Size: 5776 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 2492 3085 3311 3463 5572 1493 Size: 999 593 226 152 2109 1697 Fragments arranged by size: 2109 1697 999 593 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2072 3101 1870 Size: 73 129 1029 4545 Fragments arranged by size: 4545 1029 129 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2423 3097 4552 618 Size: 325 217 627 221 415 674 1455 1842 Fragments arranged by size: 1842 1455 674 627 415 325 221 217 BanII G_rGCy'C Cuts at: 1391 2043 2079 2241 2769 2847 3061 3095 1391 Size: 652 36 162 528 78 214 34 4072 Fragments arranged by size: 4072 652 528 214 162 78 36 34 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 5776 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2439 2486 928 Size: 971 540 47 4218 Fragments arranged by size: 4218 971 540 47 BbvCI CC'TCA_GC Cuts at: 2918 2918 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 2736 4387 Size: 107 122 71 631 605 428 636 1651 Cuts at: 4387 136 Size: 1525 Fragments arranged by size: 1651 1525 636 631 605 428 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2079 3837 4993 1790 Size: 289 1758 1156 2573 Fragments arranged by size: 2573 1758 1156 289 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2594 2846 2918 3005 3037 4436 1239 Size: 1355 252 72 87 32 1399 2579 Fragments arranged by size: 2579 1399 1355 252 87 72 32 Bpu10I CC'TnA_GC Cuts at: 2582 2630 2918 2582 Size: 48 288 5440 Fragments arranged by size: 5440 288 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2831 3901 4766 5064 2831 Size: 1070 865 298 3543 Fragments arranged by size: 3543 1070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2570 4439 882 Size: 617 1071 1869 2219 Fragments arranged by size: 2219 1869 1071 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3583 3965 4600 Size: 53 83 186 936 476 1574 382 635 Cuts at: 4600 275 Size: 1451 Fragments arranged by size: 1574 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2153 4209 4607 4881 5028 5439 2153 Size: 2056 398 274 147 411 2490 Fragments arranged by size: 2490 2056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5551 Fragments arranged by size: 5551 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2777 2855 1186 Size: 988 603 78 4107 Fragments arranged by size: 4107 988 603 78 BseYI C'CCAG_C Cuts at: 2202 3004 4979 5475 2202 Size: 802 1975 496 2503 Fragments arranged by size: 2503 1975 802 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2639 1163 Size: 573 903 4300 Fragments arranged by size: 4300 903 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2241 2847 3061 3837 3922 4993 2241 Size: 606 214 776 85 1071 3024 Fragments arranged by size: 3024 1071 776 606 214 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2079 2241 2769 2847 3061 3095 Size: 399 253 36 162 528 78 214 34 Cuts at: 3095 3837 3922 4993 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 528 399 253 214 162 85 78 36 34 BspEI T'CCGG_A Cuts at: 2153 5439 2153 Size: 3286 2490 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2389 3131 844 Size: 1545 742 3489 Fragments arranged by size: 3489 1545 742 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 2555 2603 2909 3836 4848 2555 Size: 48 306 927 1012 3483 Fragments arranged by size: 3483 1012 927 306 48 BstAPI GCAn_nnn'nTGC Cuts at: 794 2980 794 Size: 2186 3590 BstBI TT'CG_AA Cuts at: 2856 4580 2856 Size: 1724 4052 BstEII G'GTnAC_C Cuts at: 1184 2401 1184 Size: 1217 4559 BstXI CCAn_nnnn'nTGG Cuts at: 3004 3004 Size: 5776 BstYI r'GATC_y Cuts at: 1972 3048 3106 3473 3858 3875 5327 5413 5425 Size: 1076 58 367 385 17 1452 86 12 Cuts at: 5425 5771 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1076 385 367 346 86 58 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 2095 513 Size: 552 939 8 83 4194 Fragments arranged by size: 4194 939 552 83 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2361 3223 Size: 54 1425 50 275 18 402 129 862 Cuts at: 3223 4112 5741 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 862 402 275 129 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 3706 1353 Size: 719 1634 3423 Fragments arranged by size: 3423 1634 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2791 3007 4363 4737 4883 387 Size: 1064 138 1202 216 1356 374 146 1280 Fragments arranged by size: 1356 1280 1202 1064 374 216 146 138 EcoICRI GAG'CTC Cuts at: 2239 2845 3059 2239 Size: 606 214 4956 Fragments arranged by size: 4956 606 214 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2594 2696 2846 2918 3005 3037 Size: 393 641 321 102 150 72 87 32 Cuts at: 3037 3839 4436 5223 5491 1239 Size: 802 597 787 268 1524 Fragments arranged by size: 1524 802 787 641 597 393 321 268 150 102 87 72 32 EcoNI CCTnn'n_nnAGG Cuts at: 2984 2984 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 2076 2704 2788 3034 2076 Size: 628 84 246 4818 Fragments arranged by size: 4818 628 246 84 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FspI TGC'GCA Cuts at: 2690 2720 2907 4282 2690 Size: 30 187 1375 4184 Fragments arranged by size: 4184 1375 187 30 HaeII r_GCGC'y Cuts at: 1678 2012 2051 4923 1678 Size: 334 39 2872 2531 Fragments arranged by size: 2872 2531 334 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373 Size: 32 135 32 2845 32 42 32 2626 Fragments arranged by size: 2845 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 2294 748 Size: 1546 4230 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KasI G'GCGC_C Cuts at: 2008 2008 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2363 10 Size: 54 1425 343 531 3423 Fragments arranged by size: 3423 1425 531 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3734 4093 4252 518 Size: 648 2568 359 159 2042 Fragments arranged by size: 2568 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2087 2188 2896 3192 3869 5017 5262 Size: 408 73 101 708 296 677 1148 245 Cuts at: 5262 1606 Size: 2120 Fragments arranged by size: 2120 1148 708 677 408 296 245 101 73 NarI GG'CG_CC Cuts at: 2009 2009 Size: 5776 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4285 Fragments arranged by size: 4285 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 2769 1348 Size: 1421 4355 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2707 1108 Size: 1599 4177 PfoI T'CCnGG_A Cuts at: 2835 2835 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2741 2773 3845 3877 5358 5390 2741 Size: 32 1072 32 1481 32 3127 Fragments arranged by size: 3127 1481 1072 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2788 3034 2788 Size: 246 5530 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 2075 2075 Size: 5776 PstI C_TGCA'G Cuts at: 830 3033 3128 830 Size: 2203 95 3478 Fragments arranged by size: 3478 2203 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2188 2896 2188 Size: 708 5068 SacI G_AGCT'C Cuts at: 2241 2847 3061 2241 Size: 606 214 4956 Fragments arranged by size: 4956 606 214 SacII CC_GC'GG Cuts at: 2015 2015 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2072 2072 Size: 5776 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 2108 4024 5736 2108 Size: 1916 1712 2148 Fragments arranged by size: 2148 1916 1712 SfcI C'TryA_G Cuts at: 826 1045 2740 3029 3124 4259 4940 5131 826 Size: 219 1695 289 95 1135 681 191 1471 Fragments arranged by size: 1695 1471 1135 681 289 219 191 95 SfoI GGC'GCC Cuts at: 2010 2010 Size: 5776 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2846 3880 4781 5043 820 Size: 197 926 903 1034 901 262 1553 Fragments arranged by size: 1553 1034 926 903 901 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 2997 3215 513 Size: 552 939 993 218 3074 Fragments arranged by size: 3074 993 939 552 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2441 2606 3803 3820 3973 4158 4603 Size: 1255 861 165 1197 17 153 185 445 Cuts at: 4603 325 Size: 1498 Fragments arranged by size: 1498 1255 1197 861 445 185 165 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 2559 4022 Size: 274 80 33 51 1443 129 453 1463 Cuts at: 4022 5734 96 Size: 1712 138 Fragments arranged by size: 1712 1463 1443 453 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2450 2482 5736 5768 2450 Size: 32 3254 32 2458 Fragments arranged by size: 3254 2458 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2371 2371 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI