(Circular) (Six-base) MAPSORT of: pFN21AE0506.seq Check: 8201 from: 1 to: 5692 pFN21AE0506 5692 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3035 1937 Size: 1098 4594 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2113 3755 5139 5407 1632 Size: 481 1642 1384 268 1917 Fragments arranged by size: 1917 1642 1384 481 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 2244 2331 4591 5568 1203 Size: 1041 87 2260 977 1327 Fragments arranged by size: 2260 1327 1041 977 87 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 2259 1166 Size: 1093 4599 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5692 AlwNI CAG_nnn'CTG Cuts at: 2392 2821 3435 5007 2392 Size: 429 614 1572 3077 Fragments arranged by size: 3077 1572 614 429 ApaI G_GGCC'C Cuts at: 2514 2514 Size: 5692 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 3001 3227 3379 5488 1493 Size: 1508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 226 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 2779 2930 3017 1870 Size: 73 836 151 87 4545 Fragments arranged by size: 4545 836 151 87 73 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2926 3013 4468 618 Size: 325 217 627 1139 87 1455 1842 Fragments arranged by size: 1842 1455 1139 627 325 217 87 BanII G_rGCy'C Cuts at: 1391 2514 2543 3011 1391 Size: 1123 29 468 4072 Fragments arranged by size: 4072 1123 468 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4721 BbvCI CC'TCA_GC Cuts at: 2220 2220 Size: 5692 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2935 2969 3883 3917 1903 Size: 34 998 34 914 34 3678 Fragments arranged by size: 3678 998 914 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4303 136 Size: 107 122 71 631 605 2631 1525 Fragments arranged by size: 2631 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 2042 3109 2042 Size: 1067 4625 Bme1580I G_kGCm'C Cuts at: 1790 2514 2931 3753 4909 1790 Size: 724 417 822 1156 2573 Fragments arranged by size: 2573 1156 822 724 417 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2748 3423 4381 Size: 416 53 83 399 24 1331 675 958 Cuts at: 4381 442 Size: 1753 Fragments arranged by size: 1753 1331 958 675 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2668 2668 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2948 4352 1239 Size: 1709 1404 2579 Fragments arranged by size: 2579 1709 1404 Bpu10I CC'TnA_GC Cuts at: 2220 2220 Size: 5692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3817 4682 4980 3817 Size: 865 298 4529 Fragments arranged by size: 4529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2066 2135 4355 882 Size: 617 567 69 2220 2219 Fragments arranged by size: 2220 2219 617 567 69 BsaAI yAC'GTr Cuts at: 493 1204 2332 3483 493 Size: 711 1128 1151 2702 Fragments arranged by size: 2702 1151 1128 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3499 3881 4516 275 Size: 53 83 186 936 1966 382 635 1451 Fragments arranged by size: 1966 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4125 4523 4797 4944 5355 4125 Size: 398 274 147 411 4462 Fragments arranged by size: 4462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2442 2472 1178 Size: 30 165 30 1039 30 4398 Fragments arranged by size: 4398 1039 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 2767 1186 Size: 1003 578 4111 Fragments arranged by size: 4111 1003 578 BseYI C'CCAG_C Cuts at: 2279 2513 2669 4895 5391 2279 Size: 234 156 2226 496 2580 Fragments arranged by size: 2580 2226 496 234 156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1997 2291 1163 Size: 573 261 294 4564 Fragments arranged by size: 4564 573 294 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3142 3903 4931 1540 Size: 275 186 1141 761 1028 2301 Fragments arranged by size: 2301 1141 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 3753 3838 4909 2328 Size: 1425 85 1071 3111 Fragments arranged by size: 3111 1425 1071 85 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 BsmBI CGTCTCn'nnnn_ Cuts at: 2256 2573 2256 Size: 317 5375 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2514 2543 2931 3011 3753 3838 Size: 399 538 186 29 388 80 742 85 Cuts at: 3838 4909 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 538 399 388 186 85 80 29 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2849 3047 844 Size: 2005 198 3489 Fragments arranged by size: 3489 2005 198 BsrBI CCG'CTC Cuts at: 1394 1823 2083 3579 1394 Size: 429 260 1496 3507 Fragments arranged by size: 3507 1496 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 2491 4187 4361 65 Size: 1183 1243 1696 174 1396 Fragments arranged by size: 1696 1396 1243 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2115 4336 4523 1700 Size: 110 305 2221 187 2869 Fragments arranged by size: 2869 2221 305 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 2118 2855 794 Size: 1324 737 3631 Fragments arranged by size: 3631 1324 737 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 2284 1184 Size: 1100 4592 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2725 2233 Size: 492 5200 BstYI r'GATC_y Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687 Size: 1050 367 385 17 1452 86 12 346 Cuts at: 5687 1972 Size: 1977 Fragments arranged by size: 1977 1452 1050 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2148 2148 Size: 5692 BtgI C'CryG_G Cuts at: 513 1065 2004 2397 2520 513 Size: 552 939 393 123 3685 Fragments arranged by size: 3685 939 552 393 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2293 2578 487 Size: 683 56 1067 285 3601 Fragments arranged by size: 3601 1067 683 285 56 BtsI GCAGTG_nn' Cuts at: 2274 3208 4002 4022 2274 Size: 934 794 20 3944 Fragments arranged by size: 3944 934 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2185 2996 2185 Size: 811 4881 DraIII CAC_nnn'GTG Cuts at: 2032 2332 2032 Size: 300 5392 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 3139 4028 Size: 54 1425 50 275 18 489 820 889 Cuts at: 4028 5657 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 820 489 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3139 1537 Size: 275 1327 4090 Fragments arranged by size: 4090 1327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2102 2624 3622 1353 Size: 749 522 998 3423 Fragments arranged by size: 3423 998 749 522 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2463 4279 4653 4799 387 Size: 1064 138 874 1816 374 146 1280 Fragments arranged by size: 1816 1280 1064 874 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2113 2948 3755 4352 5139 5407 1239 Size: 393 481 835 807 597 787 268 1524 Fragments arranged by size: 1524 835 807 787 597 481 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2702 2702 Size: 5692 EcoO109I rG'GnC_Cy Cuts at: 2389 2861 2959 2389 Size: 472 98 5122 Fragments arranged by size: 5122 472 98 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2113 2113 Size: 5692 FspI TGC'GCA Cuts at: 2804 4198 2804 Size: 1394 4298 FspAI rTGC'GCAy Cuts at: 2804 2804 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 4839 1678 Size: 3161 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2845 2877 4333 4365 4407 Size: 32 135 32 1273 32 1456 32 42 Cuts at: 4407 4439 1373 Size: 32 2626 Fragments arranged by size: 2626 1456 1273 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3036 3282 669 Size: 988 281 1098 246 3079 Fragments arranged by size: 3079 1098 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4806 4990 1305 Size: 3501 184 2007 Fragments arranged by size: 3501 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3870 Fragments arranged by size: 3870 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2259 2375 2723 3650 4009 4168 Size: 648 996 97 116 348 927 359 159 Cuts at: 4168 518 Size: 2042 Fragments arranged by size: 2042 996 927 648 359 348 159 116 97 MspA1I CmG'CkG Cuts at: 1606 2259 2876 2968 3108 3785 4933 5178 1606 Size: 653 617 92 140 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 653 617 245 140 92 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4201 Fragments arranged by size: 4201 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 3050 4595 1132 Size: 97 1821 1545 2229 Fragments arranged by size: 2229 1821 1545 97 PasI CC'CwG_GG Cuts at: 1348 2821 1348 Size: 1473 4219 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 2413 2923 1108 Size: 1305 510 3877 Fragments arranged by size: 3877 1305 510 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PmlI CAC'GTG Cuts at: 2332 2332 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2389 2861 2959 2389 Size: 472 98 5122 Fragments arranged by size: 5122 472 98 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PspOMI G'GGCC_C Cuts at: 2510 2510 Size: 5692 PstI C_TGCA'G Cuts at: 830 3044 830 Size: 2214 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SapI GCTCTTCn'nnn_ Cuts at: 2102 2102 Size: 5692 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 3040 4175 4856 5047 826 Size: 219 1995 1135 681 191 1471 Fragments arranged by size: 1995 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 3019 1872 Size: 1147 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3796 4697 4959 820 Size: 197 926 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 2982 1517 Size: 1465 4227 StyI C'CwwG_G Cuts at: 513 1065 2004 2640 3131 513 Size: 552 939 636 491 3074 Fragments arranged by size: 3074 939 636 552 491 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2090 2293 3719 3736 3889 4074 4519 Size: 1255 510 203 1426 17 153 185 445 Cuts at: 4519 325 Size: 1498 Fragments arranged by size: 1498 1426 1255 510 445 203 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3938 5650 96 Size: 274 80 33 51 1443 1961 1712 138 Fragments arranged by size: 1961 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2442 2474 2512 2544 5652 5684 2442 Size: 32 38 32 3108 32 2450 Fragments arranged by size: 3108 2450 38 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 3017 1870 Size: 1147 4545 XmnI GAAnn'nnTTC Cuts at: 2552 2747 3821 2552 Size: 195 1074 4423 Fragments arranged by size: 4423 1074 195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI