Restriction map for
pFN21AE0556
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvaI AvrII BalI BamHI
BanI BbeI BbsI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 601 GAATTT [GA]^AATT[CT]
AhdI 149 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 717 GATCC GGATC(4/5)
ApoI 601 GAATTT [GA]^AATT[CT]
AspEI 149 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 101 TGCGCA TGC^GCA
BbrPI 664 CACGTG CAC^GTG
BfaI 556 CTAG C^TAG
BsaAI 664 CACGTG [CT]AC^GT[GA]
BsaI 361 GGTCTC GGTCTC(1/5)
Bsp1407I 314 TGTACA T^GTACA
BsrDI 196 GCAATG GCAATG(2/0)
BsrFI 43 ACCGGC [GA]^CCGG[CT]
BsrGI 314 TGTACA T^GTACA
BstAPI 702 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 201 GGTGACC G^GT[ACGT]ACC
BstPI 201 GGTGACC G^GT[ACGT]ACC
BstYI 716 AGATCC [GA]^GATC[CT]
Cfr10I 43 ACCGGC [GA]^CCGG[CT]
DpnII 717 GATC ^GATC
EaeI 369 TGGCCG [CT]^GGCC[GA]
Eam1105I 149 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 271 CTCTTC CTCTTC(1/4)
EcoNI 387 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 201 GGTGACC G^GT[ACGT]ACC
FauI 681 GCGGG CCCGC(4/6)
FspI 101 TGCGCA TGC^GCA
HphI 201 GGTGA GGTGA(8/7)
Ksp632I 271 CTCTTC CTCTTC(1/4)
MaeI 556 CTAG C^TAG
MaeII 665 ACGT A^CGT
MaeIII 202 GTGAC ^GT[ACGT]AC
MboI 717 GATC ^GATC
MboII 272 TCTTC GAAGA(8/7)
MflI 716 AGATCC [GA]^GATC[CT]
MseI 613 TTAA T^TAA
MslI 159 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 717 GATC ^GATC
PmaCI 664 CACGTG CAC^GTG
PmlI 664 CACGTG CAC^GTG
SapI 270 GCTCTTC GCTCTTC(1/4)
Sau3AI 717 GATC ^GATC
SspBI 314 TGTACA T^GTACA
Tru9I 613 TTAA T^TAA
Tsp45I 202 GTGAC ^GT[GC]AC
Tsp509I 602 AATT ^AATT
XcmI 347 CCAGGAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 716 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
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AfaI 78 GTAC GT^AC
315 GTAC
BglI 111 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
214 GCCTCAGCGGC
BsrI 243 ACTGG ACTGG(1/-1)
163 CCAGT
EcoT14I 227 CCATGG C^C[AT][AT]GG
276 CCATGG
HaeII 574 AGCGCC [GA]GCGC^[CT]
741 GGCGCT
HinfI 450 GACTC G^A[ACGT]TC
728 GACTC
NcoI 227 CCATGG C^CATGG
276 CCATGG
PleI 450 GACTC GAGTC(4/5)
728 GACTC
PpuMI 61 GGGTCCT [GA]G^G[AT]CC[CT]
280 GGGACCC
RsaI 78 GTAC GT^AC
315 GTAC
StyI 227 CCATGG C^C[AT][AT]GG
276 CCATGG
TspRI 38 CACTG CA[GC]TG[ACGT][ACGT]^
164 CAGTG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwNI ApaI AvaII
BanII BbvCI BbvI BcnI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PstI PvuII Sau96I ScrFI SfaNI
SfcI TseI