(Circular) (Six-base) MAPSORT of: pFN21AE0469.seq Check: 6987 from: 1 to: 5329 pFN21AE0469 5329 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2479 2479 Size: 5329 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3139 278 Size: 53 83 186 936 1603 2468 Fragments arranged by size: 2468 1603 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2672 1937 Size: 735 4594 Acc65I G'GTAC_C Cuts at: 2650 2650 Size: 5329 AclI AA'CG_TT Cuts at: 1563 3456 3829 1563 Size: 1893 373 3063 Fragments arranged by size: 3063 1893 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2226 3392 4776 5044 1632 Size: 594 1166 1384 268 1917 Fragments arranged by size: 1917 1384 1166 594 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5132 AflIII A'CryG_T Cuts at: 1203 4228 5205 1203 Size: 3025 977 1327 Fragments arranged by size: 3025 1327 977 AgeI A'CCGG_T Cuts at: 4160 4160 Size: 5329 AhdI GACnn_n'nnGTC Cuts at: 2072 4058 2072 Size: 1986 3343 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5329 AlwNI CAG_nnn'CTG Cuts at: 2494 3072 4644 2494 Size: 578 1572 3179 Fragments arranged by size: 3179 1572 578 ApaI G_GGCC'C Cuts at: 2014 2023 2150 2014 Size: 9 127 5193 Fragments arranged by size: 5193 127 9 ApaLI G'TGCA_C Cuts at: 3386 4542 3386 Size: 1156 4173 ApoI r'AATT_y Cuts at: 1493 2638 2864 3016 5125 1493 Size: 1145 226 152 2109 1697 Fragments arranged by size: 2109 1697 1145 226 152 AseI AT'TA_AT Cuts at: 160 1032 3883 160 Size: 872 2851 1606 Fragments arranged by size: 2851 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5329 AvaI C'yCGr_G Cuts at: 1870 1943 2654 1870 Size: 73 711 4545 Fragments arranged by size: 4545 711 73 BamHI G'GATC_C Cuts at: 2659 3026 2659 Size: 367 4962 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 2650 4105 618 Size: 325 217 627 263 600 1455 1842 Fragments arranged by size: 1842 1455 627 600 325 263 217 BanII G_rGCy'C Cuts at: 1391 2014 2023 2090 2150 2648 1391 Size: 623 9 67 60 498 4072 Fragments arranged by size: 4072 623 498 67 60 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2214 2243 928 Size: 971 315 29 4014 Fragments arranged by size: 4014 971 315 29 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3520 3554 1903 Size: 34 1583 34 3678 Fragments arranged by size: 3678 1583 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3221 4431 3221 Size: 1210 4119 BclI T'GATC_A Cuts at: 1548 1548 Size: 5329 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2018 3940 Size: 107 122 71 631 605 337 9 1922 Cuts at: 3940 136 Size: 1525 Fragments arranged by size: 1922 1525 631 605 337 122 107 71 9 BglII A'GATC_T Cuts at: 2457 5324 2457 Size: 2867 2462 BlpI GC'TnA_GC Cuts at: 2746 2746 Size: 5329 Bme1580I G_kGCm'C Cuts at: 1790 2014 2023 2055 2150 3390 4546 1790 Size: 224 9 32 95 1240 1156 2573 Fragments arranged by size: 2573 1240 1156 224 95 32 9 BmgBI CAC'GTC Cuts at: 1318 2299 1318 Size: 981 4348 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2066 2315 3060 Size: 416 53 83 399 24 649 249 745 Cuts at: 3060 4018 442 Size: 958 1753 Fragments arranged by size: 1753 958 745 649 416 399 249 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5329 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2199 3989 1239 Size: 960 1790 2579 Fragments arranged by size: 2579 1790 960 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3454 4319 4617 3454 Size: 865 298 4166 Fragments arranged by size: 4166 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3992 882 Size: 617 2493 2219 Fragments arranged by size: 2493 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3120 493 Size: 711 1916 2702 Fragments arranged by size: 2702 1916 711 BsaBI GATnn'nnATC Cuts at: 5323 5323 Size: 5329 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2068 3136 3518 4153 Size: 53 83 186 936 535 1068 382 635 Cuts at: 4153 275 Size: 1451 Fragments arranged by size: 1451 1068 936 635 535 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3762 4160 4434 4581 4992 3762 Size: 398 274 147 411 4099 Fragments arranged by size: 4099 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5104 Fragments arranged by size: 5104 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2196 2452 2582 1186 Size: 1010 256 130 3933 Fragments arranged by size: 3933 1010 256 130 BseYI C'CCAG_C Cuts at: 2474 4532 5028 2474 Size: 2058 496 2775 Fragments arranged by size: 2775 2058 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4756 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2779 3540 4568 1540 Size: 275 186 778 761 1028 2301 Fragments arranged by size: 2301 1028 778 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3390 3475 4546 3390 Size: 85 1071 4173 Fragments arranged by size: 4173 1071 85 BsmI GAATG_Cn' Cuts at: 2840 2933 2840 Size: 93 5236 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2023 2055 2090 2150 2648 3390 Size: 399 224 9 32 35 60 498 742 Cuts at: 3390 3475 4546 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 498 399 224 85 60 35 32 9 BspEI T'CCGG_A Cuts at: 4992 4992 Size: 5329 BspHI T'CATG_A Cuts at: 3218 4948 3218 Size: 1730 3599 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2479 2684 844 Size: 1635 205 3489 Fragments arranged by size: 3489 1635 205 BsrBI CCG'CTC Cuts at: 1394 1823 3216 1394 Size: 429 1393 3507 Fragments arranged by size: 3507 1393 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3824 3998 65 Size: 1183 2576 174 1396 Fragments arranged by size: 2576 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 3973 4160 1700 Size: 110 290 1873 187 2869 Fragments arranged by size: 2869 1873 290 187 110 BsrGI T'GTAC_A Cuts at: 96 2409 96 Size: 2313 3016 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5329 BssSI C'ACGA_G Cuts at: 3389 4401 3389 Size: 1012 4317 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5329 BstBI TT'CG_AA Cuts at: 4133 4133 Size: 5329 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5329 BstYI r'GATC_y Cuts at: 1972 2457 2659 3026 3411 3428 4880 4966 4978 Size: 485 202 367 385 17 1452 86 12 Cuts at: 4978 5324 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 485 385 367 346 202 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2314 2314 Size: 5329 BtgI C'CryG_G Cuts at: 513 1065 2004 2619 513 Size: 552 939 615 3223 Fragments arranged by size: 3223 939 615 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2054 487 Size: 683 56 828 3762 Fragments arranged by size: 3762 828 683 56 BtsI GCAGTG_nn' Cuts at: 2845 3639 3659 2845 Size: 794 20 4515 Fragments arranged by size: 4515 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5329 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5294 DraI TTT'AAA Cuts at: 2633 2633 Size: 5329 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4336 809 Size: 507 3020 1802 Fragments arranged by size: 3020 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2102 2452 2509 Size: 54 1425 50 275 18 272 350 57 Cuts at: 2509 2776 3665 5294 8 Size: 267 889 1629 43 Fragments arranged by size: 1629 1425 889 350 275 272 267 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2776 1537 Size: 275 964 4090 Fragments arranged by size: 4090 964 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3259 1353 Size: 1906 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3916 4290 4436 387 Size: 1064 138 2327 374 146 1280 Fragments arranged by size: 2327 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2226 3392 3989 4776 5044 1239 Size: 393 567 27 1166 597 787 268 1524 Fragments arranged by size: 1524 1166 787 597 567 393 268 27 EcoNI CCTnn'n_nnAGG Cuts at: 2181 2181 Size: 5329 EcoO109I rG'GnC_Cy Cuts at: 2010 2019 2146 2147 2010 Size: 9 127 1 5192 Fragments arranged by size: 5192 127 9 1 EcoRI G'AATT_C Cuts at: 2638 3016 2638 Size: 378 4951 FspI TGC'GCA Cuts at: 3835 3835 Size: 5329 HaeII r_GCGC'y Cuts at: 1678 4476 1678 Size: 2798 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 3970 4002 4044 Size: 32 135 32 486 32 1880 32 42 Cuts at: 4044 4076 1373 Size: 32 2626 Fragments arranged by size: 2626 1880 486 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2673 2919 669 Size: 988 281 735 246 3079 Fragments arranged by size: 3079 988 735 281 246 HindIII A'AGCT_T Cuts at: 748 2385 2444 748 Size: 1637 59 3633 Fragments arranged by size: 3633 1637 59 HpaI GTT'AAC Cuts at: 2919 2919 Size: 5329 KpnI G_GTAC'C Cuts at: 2654 2654 Size: 5329 MfeI C'AATT_G Cuts at: 2928 2928 Size: 5329 MluI A'CGCG_T Cuts at: 5205 5205 Size: 5329 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4443 4627 1305 Size: 3138 184 2007 Fragments arranged by size: 3138 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2454 2511 10 Size: 54 1425 343 622 57 2828 Fragments arranged by size: 2828 1425 622 343 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 3287 3646 3805 518 Size: 648 2121 359 159 2042 Fragments arranged by size: 2121 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2745 3422 4570 4815 1606 Size: 1139 677 1148 245 2120 Fragments arranged by size: 2120 1148 1139 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3838 Fragments arranged by size: 3838 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5329 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5329 NotI GC'GGCC_GC Cuts at: 2776 2776 Size: 5329 NspI r_CATG'y Cuts at: 1132 1229 2687 4232 1132 Size: 97 1458 1545 2229 Fragments arranged by size: 2229 1545 1458 97 PasI CC'CwG_GG Cuts at: 1348 2014 2090 1348 Size: 666 76 4587 Fragments arranged by size: 4587 666 76 PciI A'CATG_T Cuts at: 4228 4228 Size: 5329 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5329 PmeI GTTT'AAAC Cuts at: 2633 2633 Size: 5329 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3398 3430 4911 4943 3398 Size: 32 1481 32 3784 Fragments arranged by size: 3784 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5329 PsiI TTA'TAA Cuts at: 2899 2899 Size: 5329 PspOMI G'GGCC_C Cuts at: 2010 2019 2146 2010 Size: 9 127 5193 Fragments arranged by size: 5193 127 9 PstI C_TGCA'G Cuts at: 830 2346 2681 830 Size: 1516 335 3478 Fragments arranged by size: 3478 1516 335 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5329 SalI G'TCGA_C Cuts at: 1936 2671 1936 Size: 735 4594 SbfI CC_TGCA'GG Cuts at: 2681 2681 Size: 5329 ScaI AGT'ACT Cuts at: 3577 5289 3577 Size: 1712 3617 SfcI C'TryA_G Cuts at: 826 1045 2244 2342 2677 3812 4493 4684 826 Size: 219 1199 98 335 1135 681 191 1471 Fragments arranged by size: 1471 1199 1135 681 335 219 191 98 SfiI GGCCn_nnn'nGGCC Cuts at: 2018 2018 Size: 5329 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5329 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5329 SmaI CCC'GGG Cuts at: 1872 2656 1872 Size: 784 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3433 4334 4596 820 Size: 197 926 1490 901 262 1553 Fragments arranged by size: 1553 1490 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3120 493 Size: 2627 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5329 SphI G_CATG'C Cuts at: 1132 2687 1132 Size: 1555 3774 SspI AAT'ATT Cuts at: 5 52 3253 5 Size: 47 3201 2081 Fragments arranged by size: 3201 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5329 StyI C'CwwG_G Cuts at: 513 1065 2004 2524 2768 513 Size: 552 939 520 244 3074 Fragments arranged by size: 3074 939 552 520 244 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3356 3373 3526 3711 4156 325 Size: 1255 1776 17 153 185 445 1498 Fragments arranged by size: 1776 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2409 3575 5287 Size: 274 80 33 51 1443 432 1166 1712 Cuts at: 5287 96 Size: 138 Fragments arranged by size: 1712 1443 1166 432 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5289 5321 5289 Size: 32 5297 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5329 XbaI T'CTAG_A Cuts at: 2665 2665 Size: 5329 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2279 2279 Size: 5329 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5329 XmaI C'CCGG_G Cuts at: 1870 2654 1870 Size: 784 4545 XmnI GAAnn'nnTTC Cuts at: 3458 3458 Size: 5329 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3137 276 Size: 53 83 186 936 1603 2468 Fragments arranged by size: 2468 1603 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI