(Circular) (Six-base) MAPSORT of: pFN21AB7840.seq Check: 526 from: 1 to: 6550 pFN21AB7840 6550 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 4360 278 Size: 53 83 186 936 2056 768 2468 Fragments arranged by size: 2468 2056 936 768 186 83 53 AccI GT'mk_AC Cuts at: 1937 2715 3893 1937 Size: 778 1178 4594 Fragments arranged by size: 4594 1178 778 Acc65I G'GTAC_C Cuts at: 3871 3871 Size: 6550 AclI AA'CG_TT Cuts at: 1563 4677 5050 1563 Size: 3114 373 3063 Fragments arranged by size: 3114 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3471 4613 5997 6265 1632 Size: 1839 1142 1384 268 1917 Fragments arranged by size: 1917 1839 1384 1142 268 AfeI AGC'GCT Cuts at: 2870 2870 Size: 6550 AflII C'TTAA_G Cuts at: 820 1017 2303 820 Size: 197 1286 5067 Fragments arranged by size: 5067 1286 197 AflIII A'CryG_T Cuts at: 1203 3421 5449 6426 1203 Size: 2218 2028 977 1327 Fragments arranged by size: 2218 2028 1327 977 AgeI A'CCGG_T Cuts at: 5381 5381 Size: 6550 AhdI GACnn_n'nnGTC Cuts at: 5279 5279 Size: 6550 AleI CACnn'nnGTG Cuts at: 1166 2424 1166 Size: 1258 5292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2455 3018 3728 3752 1184 Size: 1271 563 710 24 3982 Fragments arranged by size: 3982 1271 710 563 24 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3315 3347 3315 Size: 32 6518 AlwNI CAG_nnn'CTG Cuts at: 2401 2674 3269 4293 5865 2401 Size: 273 595 1024 1572 3086 Fragments arranged by size: 3086 1572 1024 595 273 ApaI G_GGCC'C Cuts at: 3046 3526 3046 Size: 480 6070 ApaLI G'TGCA_C Cuts at: 2177 2791 3477 4607 5763 2177 Size: 614 686 1130 1156 2964 Fragments arranged by size: 2964 1156 1130 686 614 ApoI r'AATT_y Cuts at: 1493 3859 4085 4237 6346 1493 Size: 2366 226 152 2109 1697 Fragments arranged by size: 2366 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5104 160 Size: 872 4072 1606 Fragments arranged by size: 4072 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 AvaI C'yCGr_G Cuts at: 1870 1943 3093 3344 3825 3875 1870 Size: 73 1150 251 481 50 4545 Fragments arranged by size: 4545 1150 481 251 73 50 AvrII C'CTAG_G Cuts at: 3694 3694 Size: 6550 BamHI G'GATC_C Cuts at: 3880 4247 3880 Size: 367 6183 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 2185 2290 2627 2637 Size: 325 217 627 241 157 105 337 10 Cuts at: 2637 3236 3871 5326 618 Size: 599 635 1455 1842 Fragments arranged by size: 1842 1455 635 627 599 337 325 241 217 157 105 10 BanII G_rGCy'C Cuts at: 1391 2475 3046 3120 3279 3301 3333 3526 3869 Size: 1084 571 74 159 22 32 193 343 Cuts at: 3869 1391 Size: 4072 Fragments arranged by size: 4072 1084 571 343 193 159 74 32 22 BbeI G_GCGC'C Cuts at: 2032 2032 Size: 6550 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5579 BbvCI CC'TCA_GC Cuts at: 2802 2802 Size: 6550 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4741 4775 1903 Size: 34 2804 34 3678 Fragments arranged by size: 3678 2804 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4442 5652 4442 Size: 1210 5340 BclI T'GATC_A Cuts at: 1548 1548 Size: 6550 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2619 5161 136 Size: 107 122 71 631 605 947 2542 1525 Fragments arranged by size: 2542 1525 947 631 605 122 107 71 BglII A'GATC_T Cuts at: 2136 6545 2136 Size: 4409 2141 BlpI GC'TnA_GC Cuts at: 3967 3967 Size: 6550 Bme1580I G_kGCm'C Cuts at: 1790 2108 2181 2295 2795 3046 3481 3526 4611 Size: 318 73 114 500 251 435 45 1085 Cuts at: 4611 5767 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1085 500 435 318 251 114 73 45 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6550 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2559 3335 4281 Size: 416 53 83 399 24 1142 776 946 Cuts at: 4281 5239 442 Size: 958 1753 Fragments arranged by size: 1753 1142 958 946 776 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6550 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3295 3315 3295 Size: 20 6530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 2149 3111 3169 3625 3637 5210 1239 Size: 795 115 962 58 456 12 1573 2579 Fragments arranged by size: 2579 1573 962 795 456 115 58 12 Bpu10I CC'TnA_GC Cuts at: 2802 3744 2802 Size: 942 5608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4675 5540 5838 4675 Size: 865 298 5387 Fragments arranged by size: 5387 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3215 3755 5213 882 Size: 617 1716 540 1458 2219 Fragments arranged by size: 2219 1716 1458 617 540 BsaAI yAC'GTr Cuts at: 493 1204 2664 4341 493 Size: 711 1460 1677 2702 Fragments arranged by size: 2702 1677 1460 711 BsaBI GATnn'nnATC Cuts at: 6544 6544 Size: 6550 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2029 2725 3589 4357 Size: 53 83 186 936 496 696 864 768 Cuts at: 4357 4739 5374 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 864 768 696 635 496 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4983 5381 5655 5802 6213 4983 Size: 398 274 147 411 5320 Fragments arranged by size: 5320 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 3296 3315 3326 Size: 30 165 30 828 30 1035 19 11 Cuts at: 3326 3345 1178 Size: 19 4383 Fragments arranged by size: 4383 1035 828 165 30 30 30 19 19 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2291 2471 2579 3287 1186 Size: 994 111 180 108 708 4449 Fragments arranged by size: 4449 994 708 180 111 108 BseYI C'CCAG_C Cuts at: 3049 3489 3527 5753 6249 3049 Size: 440 38 2226 496 3350 Fragments arranged by size: 3350 2226 496 440 38 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2387 2425 2637 3748 1163 Size: 573 651 38 212 1111 3965 Fragments arranged by size: 3965 1111 651 573 212 38 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4000 4761 5789 1540 Size: 275 186 1405 594 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 594 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 2475 2795 2955 3416 3481 4611 4696 5767 Size: 294 320 160 461 65 1130 85 1071 Cuts at: 5767 2181 Size: 2964 Fragments arranged by size: 2964 1130 1071 461 320 294 160 85 65 BsmI GAATG_Cn' Cuts at: 2744 4061 4154 2744 Size: 1317 93 5140 Fragments arranged by size: 5140 1317 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2181 2295 2475 2795 2955 3046 Size: 399 318 73 114 180 320 160 91 Cuts at: 3046 3120 3279 3301 3333 3416 3481 3526 3869 Size: 74 159 22 32 83 65 45 343 Cuts at: 3869 4611 4696 5767 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 320 318 180 160 159 114 91 85 83 74 73 65 45 32 22 BspEI T'CCGG_A Cuts at: 6213 6213 Size: 6550 BspHI T'CATG_A Cuts at: 4439 6169 4439 Size: 1730 4820 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2305 3387 3905 844 Size: 1461 1082 518 3489 Fragments arranged by size: 3489 1461 1082 518 BsrBI CCG'CTC Cuts at: 1394 1823 2034 4437 1394 Size: 429 211 2403 3507 Fragments arranged by size: 3507 2403 429 211 BsrDI GCAATG_nn' Cuts at: 65 1248 2146 3762 5045 5219 65 Size: 1183 898 1616 1283 174 1396 Fragments arranged by size: 1616 1396 1283 1183 898 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3155 5194 5381 1700 Size: 110 1345 2039 187 2869 Fragments arranged by size: 2869 2039 1345 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6550 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6550 BssSI C'ACGA_G Cuts at: 4610 5622 4610 Size: 1012 5538 BstAPI GCAn_nnn'nTGC Cuts at: 794 2176 2185 2401 2410 2606 794 Size: 1382 9 216 9 196 4738 Fragments arranged by size: 4738 1382 216 196 9 9 BstBI TT'CG_AA Cuts at: 5354 5354 Size: 6550 BstEII G'GTnAC_C Cuts at: 1184 2539 1184 Size: 1355 5195 BstYI r'GATC_y Cuts at: 1972 2136 3880 4247 4632 4649 6101 6187 6199 Size: 164 1744 367 385 17 1452 86 12 Cuts at: 6199 6545 1972 Size: 346 1977 Fragments arranged by size: 1977 1744 1452 385 367 346 164 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3270 3270 Size: 6550 BtgI C'CryG_G Cuts at: 513 1065 2122 2159 513 Size: 552 1057 37 4904 Fragments arranged by size: 4904 1057 552 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2581 487 Size: 683 56 1355 4456 Fragments arranged by size: 4456 1355 683 56 BtsI GCAGTG_nn' Cuts at: 2108 2181 2607 2896 3343 3750 4066 4860 4880 Size: 73 426 289 447 407 316 794 20 Cuts at: 4880 2108 Size: 3778 Fragments arranged by size: 3778 794 447 426 407 316 289 73 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6550 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6515 DraI TTT'AAA Cuts at: 3854 3854 Size: 6550 DraIII CAC_nnn'GTG Cuts at: 2185 2185 Size: 6550 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5557 809 Size: 507 4241 1802 Fragments arranged by size: 4241 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3367 3997 4886 Size: 54 1425 50 275 18 1537 630 889 Cuts at: 4886 6515 8 Size: 1629 43 Fragments arranged by size: 1629 1537 1425 889 630 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3997 1537 Size: 275 2185 4090 Fragments arranged by size: 4090 2185 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2448 4480 1353 Size: 1095 2032 3423 Fragments arranged by size: 3423 2032 1095 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5137 5511 5657 387 Size: 1064 138 3548 374 146 1280 Fragments arranged by size: 3548 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2473 2473 Size: 6550 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2149 3111 3169 3471 3625 3637 Size: 393 402 115 962 58 302 154 12 Cuts at: 3637 4613 5210 5997 6265 1239 Size: 976 597 787 268 1524 Fragments arranged by size: 1524 976 962 787 597 402 393 302 268 154 115 58 12 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2342 Size: 6550 EcoO109I rG'GnC_Cy Cuts at: 2019 2062 2353 3043 3266 3522 3523 2019 Size: 43 291 690 223 256 1 5046 Fragments arranged by size: 5046 690 291 256 223 43 1 EcoRI G'AATT_C Cuts at: 3859 4237 3859 Size: 378 6172 FspI TGC'GCA Cuts at: 2528 5056 2528 Size: 2528 4022 FspAI rTGC'GCAy Cuts at: 2528 2528 Size: 6550 HaeII r_GCGC'y Cuts at: 1678 2032 2239 2872 3257 3496 5697 1678 Size: 354 207 633 385 239 2201 2531 Fragments arranged by size: 2531 2201 633 385 354 239 207 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 3066 3098 3599 Size: 32 135 32 671 32 791 32 501 Cuts at: 3599 3617 3631 3649 3690 3722 3802 3834 5191 Size: 18 14 18 41 32 80 32 1357 Cuts at: 5191 5223 5265 5297 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1357 791 671 501 135 80 42 41 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 2365 2683 3894 4140 669 Size: 988 281 427 318 1211 246 3079 Fragments arranged by size: 3079 1211 988 427 318 281 246 HindIII A'AGCT_T Cuts at: 748 2850 748 Size: 2102 4448 HpaI GTT'AAC Cuts at: 4140 4140 Size: 6550 KasI G'GCGC_C Cuts at: 2028 2028 Size: 6550 KpnI G_GTAC'C Cuts at: 3875 3875 Size: 6550 MfeI C'AATT_G Cuts at: 4149 4149 Size: 6550 MluI A'CGCG_T Cuts at: 3421 6426 3421 Size: 3005 3545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2274 5664 5848 1305 Size: 969 3390 184 2007 Fragments arranged by size: 3390 2007 969 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4728 Fragments arranged by size: 4728 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2424 2600 3000 3755 4508 4867 5026 Size: 648 1258 176 400 755 753 359 159 Cuts at: 5026 518 Size: 2042 Fragments arranged by size: 2042 1258 755 753 648 400 359 176 159 MspA1I CmG'CkG Cuts at: 1606 2161 2334 2398 2410 2494 3015 3147 3966 Size: 555 173 64 12 84 521 132 819 Cuts at: 3966 4643 5791 6036 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 819 677 555 521 245 173 132 84 64 12 NaeI GCC'GGC Cuts at: 3157 3157 Size: 6550 NarI GG'CG_CC Cuts at: 2029 2029 Size: 6550 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5998 NdeI CA'TA_TG Cuts at: 387 387 Size: 6550 NgoMIV G'CCGG_C Cuts at: 3155 3155 Size: 6550 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6550 NotI GC'GGCC_GC Cuts at: 3997 3997 Size: 6550 NspI r_CATG'y Cuts at: 1132 1229 2174 3005 3908 5453 1132 Size: 97 945 831 903 1545 2229 Fragments arranged by size: 2229 1545 945 903 831 97 PasI CC'CwG_GG Cuts at: 1348 3519 3792 1348 Size: 2171 273 4106 Fragments arranged by size: 4106 2171 273 PciI A'CATG_T Cuts at: 5449 5449 Size: 6550 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6550 PfoI T'CCnGG_A Cuts at: 2021 2021 Size: 6550 PmeI GTTT'AAAC Cuts at: 3854 3854 Size: 6550 PmlI CAC'GTG Cuts at: 2664 2664 Size: 6550 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3315 3347 4619 4651 6132 6164 3315 Size: 32 1272 32 1481 32 3701 Fragments arranged by size: 3701 1481 1272 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2019 2062 2353 2019 Size: 43 291 6216 Fragments arranged by size: 6216 291 43 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6550 PsiI TTA'TAA Cuts at: 4120 4120 Size: 6550 PspOMI G'GGCC_C Cuts at: 3042 3522 3042 Size: 480 6070 PstI C_TGCA'G Cuts at: 830 3104 3902 830 Size: 2274 798 3478 Fragments arranged by size: 3478 2274 798 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6550 PvuII CAG'CTG Cuts at: 2334 2398 2410 3147 2334 Size: 64 12 737 5737 Fragments arranged by size: 5737 737 64 12 SacI G_AGCT'C Cuts at: 2475 2475 Size: 6550 SacII CC_GC'GG Cuts at: 2162 2162 Size: 6550 SalI G'TCGA_C Cuts at: 1936 3892 1936 Size: 1956 4594 SanDI GG'GwC_CC Cuts at: 2019 2019 Size: 6550 SbfI CC_TGCA'GG Cuts at: 3902 3902 Size: 6550 ScaI AGT'ACT Cuts at: 2438 2933 4798 6510 2438 Size: 495 1865 1712 2478 Fragments arranged by size: 2478 1865 1712 495 SfcI C'TryA_G Cuts at: 826 1045 2092 3025 3100 3261 3898 5033 5714 Size: 219 1047 933 75 161 637 1135 681 Cuts at: 5714 5905 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1047 933 681 637 219 191 161 75 SfoI GGC'GCC Cuts at: 2030 2030 Size: 6550 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6550 SmaI CCC'GGG Cuts at: 1872 3877 1872 Size: 2005 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 3093 4654 5555 5817 820 Size: 197 926 360 790 1561 901 262 1553 Fragments arranged by size: 1561 1553 926 901 790 360 262 197 SnaBI TAC'GTA Cuts at: 493 4341 493 Size: 3848 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6550 SphI G_CATG'C Cuts at: 1132 2174 3005 3908 1132 Size: 1042 831 903 3774 Fragments arranged by size: 3774 1042 903 831 SspI AAT'ATT Cuts at: 5 52 4474 5 Size: 47 4422 2081 Fragments arranged by size: 4422 2081 47 StuI AGG'CCT Cuts at: 1517 2301 2622 3078 1517 Size: 784 321 456 4989 Fragments arranged by size: 4989 784 456 321 StyI C'CwwG_G Cuts at: 513 1065 2968 3106 3694 3989 513 Size: 552 1903 138 588 295 3074 Fragments arranged by size: 3074 1903 588 552 295 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2278 2615 2655 3392 3532 3594 4577 Size: 1255 698 337 40 737 140 62 983 Cuts at: 4577 4594 4747 4932 5377 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 983 737 698 445 337 185 153 140 62 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2436 2931 4796 Size: 274 80 33 51 1443 459 495 1865 Cuts at: 4796 6508 96 Size: 1712 138 Fragments arranged by size: 1865 1712 1443 495 459 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2231 2263 3296 3328 3460 3492 6510 6542 2231 Size: 32 1033 32 132 32 3018 32 2239 Fragments arranged by size: 3018 2239 1033 132 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2351 1316 Size: 1035 5515 XbaI T'CTAG_A Cuts at: 3886 3886 Size: 6550 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3322 3322 Size: 6550 XhoI C'TCGA_G Cuts at: 1943 3093 1943 Size: 1150 5400 XmaI C'CCGG_G Cuts at: 1870 3875 1870 Size: 2005 4545 XmnI GAAnn'nnTTC Cuts at: 4679 4679 Size: 6550 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 4358 276 Size: 53 83 186 936 2056 768 2468 Fragments arranged by size: 2468 2056 936 768 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI NruI NsiI PacI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI