(Circular) (Six-base) MAPSORT of: pFN21AE0368.seq Check: 6510 from: 1 to: 6670 pFN21AE0368 6670 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4480 278 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2407 2565 4013 1937 Size: 470 158 1448 4594 Fragments arranged by size: 4594 1448 470 158 Acc65I G'GTAC_C Cuts at: 3991 3991 Size: 6670 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4733 6117 6385 1632 Size: 3101 1384 268 1917 Fragments arranged by size: 3101 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2243 820 Size: 197 1226 5247 Fragments arranged by size: 5247 1226 197 AflIII A'CryG_T Cuts at: 1203 5569 6546 1203 Size: 4366 977 1327 Fragments arranged by size: 4366 1327 977 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 5399 5399 Size: 6670 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6670 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6670 AlwNI CAG_nnn'CTG Cuts at: 2487 3247 4413 5985 2487 Size: 760 1166 1572 3172 Fragments arranged by size: 3172 1572 1166 760 ApaLI G'TGCA_C Cuts at: 4727 5883 4727 Size: 1156 5514 ApoI r'AATT_y Cuts at: 1493 3461 3531 3979 4205 4357 6466 1493 Size: 1968 70 448 226 152 2109 1697 Fragments arranged by size: 2109 1968 1697 448 226 152 70 AseI AT'TA_AT Cuts at: 160 1032 3221 5224 160 Size: 872 2189 2003 1606 Fragments arranged by size: 2189 2003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2074 3855 3889 3995 1870 Size: 73 119 12 1781 34 106 4545 Fragments arranged by size: 4545 1781 119 106 73 34 12 BamHI G'GATC_C Cuts at: 4000 4367 4000 Size: 367 6303 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2199 3991 5446 618 Size: 325 217 627 412 1792 1455 1842 Fragments arranged by size: 1842 1792 1455 627 412 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2145 3132 3254 3816 3862 3989 1391 Size: 678 76 987 122 562 46 127 4072 Fragments arranged by size: 4072 987 678 562 127 122 76 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2396 928 Size: 971 497 5202 Fragments arranged by size: 5202 971 497 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4861 4895 1903 Size: 34 2924 34 3678 Fragments arranged by size: 3678 2924 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4562 5772 3758 Size: 804 1210 4656 Fragments arranged by size: 4656 1210 804 BclI T'GATC_A Cuts at: 1548 1548 Size: 6670 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5281 136 Size: 107 122 71 631 605 3609 1525 Fragments arranged by size: 3609 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6665 6665 Size: 6670 BlpI GC'TnA_GC Cuts at: 3248 4087 3248 Size: 839 5831 Bme1580I G_kGCm'C Cuts at: 1790 2420 3125 4731 5887 1790 Size: 630 705 1606 1156 2573 Fragments arranged by size: 2573 1606 1156 705 630 BmgBI CAC'GTC Cuts at: 1318 2206 1318 Size: 888 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3243 3649 4401 Size: 416 53 83 399 24 1826 406 752 Cuts at: 4401 5359 442 Size: 958 1753 Fragments arranged by size: 1826 1753 958 752 416 406 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2116 2534 3807 3822 5330 1239 Size: 877 418 1273 15 1508 2579 Fragments arranged by size: 2579 1508 1273 877 418 15 Bpu10I CC'TnA_GC Cuts at: 2068 3345 2068 Size: 1277 5393 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3660 4795 5660 5958 3660 Size: 1135 865 298 4372 Fragments arranged by size: 4372 1135 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5333 882 Size: 617 3834 2219 Fragments arranged by size: 3834 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4461 493 Size: 711 3257 2702 Fragments arranged by size: 3257 2702 711 BsaBI GATnn'nnATC Cuts at: 2883 6664 2883 Size: 3781 2889 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4477 4859 5494 275 Size: 53 83 186 936 2944 382 635 1451 Fragments arranged by size: 2944 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2460 5103 5501 5775 5922 6333 2460 Size: 2643 398 274 147 411 2797 Fragments arranged by size: 2797 2643 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2149 2179 3820 3850 1178 Size: 30 165 30 746 30 1641 30 3998 Fragments arranged by size: 3998 1641 746 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2064 3663 1186 Size: 878 1599 4193 Fragments arranged by size: 4193 1599 878 BseYI C'CCAG_C Cuts at: 2778 2849 3725 5873 6369 2778 Size: 71 876 2148 496 3079 Fragments arranged by size: 3079 2148 876 496 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2473 1163 Size: 573 737 5360 Fragments arranged by size: 5360 737 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 4120 4881 5909 1540 Size: 275 186 36 2083 761 1028 2301 Fragments arranged by size: 2301 2083 1028 761 275 186 36 BsiHKAI G_wGCw'C Cuts at: 2656 3132 3816 3862 4731 4816 5887 2656 Size: 476 684 46 869 85 1071 3439 Fragments arranged by size: 3439 1071 869 684 476 85 46 BsmI GAATG_Cn' Cuts at: 2861 3596 4181 4274 2861 Size: 735 585 93 5257 Fragments arranged by size: 5257 735 585 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2176 2176 Size: 6670 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2145 2420 2656 3125 3132 3254 Size: 399 279 76 275 236 469 7 122 Cuts at: 3254 3816 3862 3989 4731 4816 5887 1391 Size: 562 46 127 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 562 469 399 279 275 236 127 122 85 76 46 7 BspEI T'CCGG_A Cuts at: 2460 6333 2460 Size: 3873 2797 BspHI T'CATG_A Cuts at: 4559 6289 4559 Size: 1730 4940 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2419 3403 3721 3789 4025 844 Size: 1575 984 318 68 236 3489 Fragments arranged by size: 3489 1575 984 318 236 68 BsrBI CCG'CTC Cuts at: 1394 1823 2100 4557 1394 Size: 429 277 2457 3507 Fragments arranged by size: 3507 2457 429 277 BsrDI GCAATG_nn' Cuts at: 65 1248 2561 5165 5339 65 Size: 1183 1313 2604 174 1396 Fragments arranged by size: 2604 1396 1313 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2196 5314 5501 1700 Size: 110 275 111 3118 187 2869 Fragments arranged by size: 3118 2869 275 187 111 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6670 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 3042 4730 5742 3042 Size: 1688 1012 3970 Fragments arranged by size: 3970 1688 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2747 794 Size: 1953 4717 BstBI TT'CG_AA Cuts at: 2968 5474 2968 Size: 2506 4164 BstEII G'GTnAC_C Cuts at: 1184 3901 1184 Size: 2717 3953 BstXI CCAn_nnnn'nTGG Cuts at: 3758 3798 3758 Size: 40 6630 BstYI r'GATC_y Cuts at: 1972 3832 4000 4367 4752 4769 6221 6307 6319 Size: 1860 168 367 385 17 1452 86 12 Cuts at: 6319 6665 1972 Size: 346 1977 Fragments arranged by size: 1977 1860 1452 385 367 346 168 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2538 2784 2538 Size: 246 6424 BtgI C'CryG_G Cuts at: 513 1065 2004 2569 3426 513 Size: 552 939 565 857 3757 Fragments arranged by size: 3757 939 857 565 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3182 487 Size: 683 56 1956 3975 Fragments arranged by size: 3975 1956 683 56 BtsI GCAGTG_nn' Cuts at: 2601 3281 4186 4980 5000 2601 Size: 680 905 794 20 4271 Fragments arranged by size: 4271 905 794 680 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2238 2273 529 Size: 35 1674 35 4926 Fragments arranged by size: 4926 1674 35 35 DraI TTT'AAA Cuts at: 3974 3974 Size: 6670 DraIII CAC_nnn'GTG Cuts at: 2517 3022 2517 Size: 505 6165 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2222 5677 809 Size: 507 906 3455 1802 Fragments arranged by size: 3455 1802 906 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2658 4117 Size: 54 1425 50 275 18 204 624 1459 Cuts at: 4117 5006 6635 8 Size: 889 1629 43 Fragments arranged by size: 1629 1459 1425 889 624 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 4117 1537 Size: 275 222 2083 4090 Fragments arranged by size: 4090 2083 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 2313 4600 1353 Size: 960 2287 3423 Fragments arranged by size: 3423 2287 960 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2061 5257 5631 5777 387 Size: 1064 138 472 3196 374 146 1280 Fragments arranged by size: 3196 1280 1064 472 374 146 138 EcoICRI GAG'CTC Cuts at: 3130 3814 3860 3130 Size: 684 46 5940 Fragments arranged by size: 5940 684 46 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2116 2534 3807 3822 4733 5330 6117 Size: 393 484 418 1273 15 911 597 787 Cuts at: 6117 6385 1239 Size: 268 1524 Fragments arranged by size: 1524 1273 911 787 597 484 418 393 268 15 EcoNI CCTnn'n_nnAGG Cuts at: 2462 2462 Size: 6670 EcoO109I rG'GnC_Cy Cuts at: 3733 3733 Size: 6670 EcoRI G'AATT_C Cuts at: 3461 3979 4357 3461 Size: 518 378 5774 Fragments arranged by size: 5774 518 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2258 2258 Size: 6670 FspI TGC'GCA Cuts at: 5176 5176 Size: 6670 HaeII r_GCGC'y Cuts at: 1678 2095 2157 3552 5817 1678 Size: 417 62 1395 2265 2531 Fragments arranged by size: 2531 2265 1395 417 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2998 3030 3163 3195 3844 Size: 32 135 32 1426 32 133 32 649 Cuts at: 3844 3876 5311 5343 5385 5417 1373 Size: 32 1435 32 42 32 2626 Fragments arranged by size: 2626 1435 1426 649 135 133 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2408 4014 4260 669 Size: 988 281 470 1606 246 3079 Fragments arranged by size: 3079 1606 988 470 281 246 HindIII A'AGCT_T Cuts at: 748 2357 748 Size: 1609 5061 HpaI GTT'AAC Cuts at: 4260 4260 Size: 6670 KpnI G_GTAC'C Cuts at: 3995 3995 Size: 6670 MfeI C'AATT_G Cuts at: 4269 4269 Size: 6670 MluI A'CGCG_T Cuts at: 6546 6546 Size: 6670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5784 5968 1305 Size: 4479 184 2007 Fragments arranged by size: 4479 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2660 10 Size: 54 1425 343 828 4020 Fragments arranged by size: 4020 1425 828 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3477 3712 4628 4987 5146 518 Size: 648 2311 235 916 359 159 2042 Fragments arranged by size: 2311 2042 916 648 359 235 159 MspA1I CmG'CkG Cuts at: 1606 4086 4763 5911 6156 1606 Size: 2480 677 1148 245 2120 Fragments arranged by size: 2480 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2198 2198 Size: 6670 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5179 Fragments arranged by size: 5179 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6670 NgoMIV G'CCGG_C Cuts at: 2196 2196 Size: 6670 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 4117 4117 Size: 6670 NspI r_CATG'y Cuts at: 1132 1229 4028 5573 1132 Size: 97 2799 1545 2229 Fragments arranged by size: 2799 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2836 1348 Size: 1488 5182 PciI A'CATG_T Cuts at: 5569 5569 Size: 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6670 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4739 4771 6252 6284 4739 Size: 32 1481 32 5125 Fragments arranged by size: 5125 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3733 3733 Size: 6670 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6670 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PspXI vC'TCGA_Gb Cuts at: 3889 3889 Size: 6670 PstI C_TGCA'G Cuts at: 830 3288 3400 4022 830 Size: 2458 112 622 3478 Fragments arranged by size: 3478 2458 622 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 SacI G_AGCT'C Cuts at: 3132 3816 3862 3132 Size: 684 46 5940 Fragments arranged by size: 5940 684 46 SalI G'TCGA_C Cuts at: 1936 2406 4012 1936 Size: 470 1606 4594 Fragments arranged by size: 4594 1606 470 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 3198 3371 4918 6630 3198 Size: 173 1547 1712 3238 Fragments arranged by size: 3238 1712 1547 173 SfcI C'TryA_G Cuts at: 826 1045 3284 3396 4018 5153 5834 6025 826 Size: 219 2239 112 622 1135 681 191 1471 Fragments arranged by size: 2239 1471 1135 681 622 219 191 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 2076 3997 1872 Size: 204 1921 4545 Fragments arranged by size: 4545 1921 204 SmlI C'TyrA_G Cuts at: 820 1017 1943 2243 3675 3889 4774 5675 5937 Size: 197 926 300 1432 214 885 901 262 Cuts at: 5937 820 Size: 1553 Fragments arranged by size: 1553 1432 926 901 885 300 262 214 197 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6670 SphI G_CATG'C Cuts at: 1132 4028 1132 Size: 2896 3774 SrfI GCCC'GGGC Cuts at: 2076 2076 Size: 6670 SspI AAT'ATT Cuts at: 5 52 3513 4594 5 Size: 47 3461 1081 2081 Fragments arranged by size: 3461 2081 1081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6670 StyI C'CwwG_G Cuts at: 513 1065 2004 2175 4109 513 Size: 552 939 171 1934 3074 Fragments arranged by size: 3074 1934 939 552 171 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2588 4697 4714 4867 5052 5497 325 Size: 1255 1008 2109 17 153 185 445 1498 Fragments arranged by size: 2109 1498 1255 1008 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3196 3369 4916 Size: 274 80 33 51 1443 1219 173 1547 Cuts at: 4916 6628 96 Size: 1712 138 Fragments arranged by size: 1712 1547 1443 1219 274 173 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2149 2181 6630 6662 2149 Size: 32 4449 32 2157 Fragments arranged by size: 4449 2157 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6670 XbaI T'CTAG_A Cuts at: 3085 4006 3085 Size: 921 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2876 2876 Size: 6670 XhoI C'TCGA_G Cuts at: 1943 3889 1943 Size: 1946 4724 XmaI C'CCGG_G Cuts at: 1870 2074 3995 1870 Size: 204 1921 4545 Fragments arranged by size: 4545 1921 204 XmnI GAAnn'nnTTC Cuts at: 4799 4799 Size: 6670 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4478 276 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI