Restriction map for
pFN21AE0193
(Circular) (Six-base) MAPSORT of: pFN21AE0193.seq Check: 996 from: 1 to: 5782
pFN21AE0193 5782 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2329 2329
Size: 5782
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2478 3125 1937
Size: 541 647 4594
Fragments arranged by size:
4594 647 541
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 2144 3909 4282 1563
Size: 581 1765 373 3063
Fragments arranged by size:
3063 1765 581 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3845 5229 5497 1632
Size: 2213 1384 268 1917
Fragments arranged by size:
2213 1917 1384 268
AfeI AGC'GCT
Cuts at: 3074 3074
Size: 5782
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2771 1184
Size: 1587 4195
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2567 2599 2567
Size: 32 5750
AlwNI CAG_nnn'CTG
Cuts at: 2432 3525 5097 2432
Size: 1093 1572 3117
Fragments arranged by size:
3117 1572 1093
ApaLI G'TGCA_C
Cuts at: 2342 3839 4995 2342
Size: 1497 1156 3129
Fragments arranged by size:
3129 1497 1156
ApoI r'AATT_y
Cuts at: 1493 2453 3091 3317 3469 5578 1493
Size: 960 638 226 152 2109 1697
Fragments arranged by size:
2109 1697 960 638 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 3107 1870
Size: 73 121 1043 4545
Fragments arranged by size:
4545 1043 121 73
BamHI G'GATC_C
Cuts at: 3112 3479 3112
Size: 367 5415
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2291 2308 2792 3103 4558
Size: 325 217 627 504 17 484 311 1455
Cuts at: 4558 618
Size: 1842
Fragments arranged by size:
1842 1455 627 504 484 325 311 217
17
BanII G_rGCy'C
Cuts at: 1391 3101 1391
Size: 1710 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BbvCI CC'TCA_GC
Cuts at: 2588 2588
Size: 5782
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 2596 1548
Size: 1048 4734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4393 136
Size: 107 122 71 631 605 337 2384 1525
Fragments arranged by size:
2384 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 3199 3199
Size: 5782
Bme1580I G_kGCm'C
Cuts at: 1790 2201 2346 2760 3843 4999 1790
Size: 411 145 414 1083 1156 2573
Fragments arranged by size:
2573 1156 1083 414 411 145
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 2338 2817
Size: 416 53 83 399 24 828 93 479
Cuts at: 2817 3513 4471 442
Size: 696 958 1753
Fragments arranged by size:
1753 958 828 696 479 416 399 93
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2517 4442 1239
Size: 1278 1925 2579
Fragments arranged by size:
2579 1925 1278
Bpu10I CC'TnA_GC
Cuts at: 2588 2588
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2460 2490 2567 2597 1178
Size: 30 165 30 1057 30 77 30 4363
Fragments arranged by size:
4363 1057 165 77 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2788 1186
Size: 1324 278 4180
Fragments arranged by size:
4180 1324 278
BseYI C'CCAG_C
Cuts at: 2464 2626 4985 5481 2464
Size: 162 2359 496 2765
Fragments arranged by size:
2765 2359 496 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2620 1163
Size: 573 884 4325
Fragments arranged by size:
4325 884 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 2346 3843 3928 4999 2169
Size: 177 1497 85 1071 2952
Fragments arranged by size:
2952 1497 1071 177 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2201 2346 2760 3101 3843 3928
Size: 399 379 32 145 414 341 742 85
Cuts at: 3928 4999 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 414 399 379 341 145
85 32
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 2599 2827 3671 5401 2599
Size: 228 844 1730 2980
Fragments arranged by size:
2980 1730 844 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2329 3137 844
Size: 1485 808 3489
Fragments arranged by size:
3489 1485 808
BsrBI CCG'CTC
Cuts at: 1394 1823 2605 3669 1394
Size: 429 782 1064 3507
Fragments arranged by size:
3507 1064 782 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2239 4277 4451 65
Size: 1183 991 2038 174 1396
Fragments arranged by size:
2038 1396 1183 991 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4426 4613 1700
Size: 110 2616 187 2869
Fragments arranged by size:
2869 2616 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5782
BssSI C'ACGA_G
Cuts at: 3842 4854 3842
Size: 1012 4770
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 4586 4586
Size: 5782
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5782
BstYI r'GATC_y
Cuts at: 1972 2812 3112 3479 3864 3881 5333 5419 5431
Size: 840 300 367 385 17 1452 86 12
Cuts at: 5431 5777 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 840 385 367 346 300 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2238 2356 2568 2776 513
Size: 552 939 234 118 212 208 3519
Fragments arranged by size:
3519 939 552 234 212 208 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2065 487
Size: 683 56 839 4204
Fragments arranged by size:
4204 839 683 56
BtsI GCAGTG_nn'
Cuts at: 2048 2951 3298 4092 4112 2048
Size: 903 347 794 20 3718
Fragments arranged by size:
3718 903 794 347 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747
Size: 54 1425 50 275 18 1399 889 1629
Cuts at: 5747 8
Size: 43
Fragments arranged by size:
1629 1425 1399 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 2874 2984 3712 1353
Size: 1160 361 110 728 3423
Fragments arranged by size:
3423 1160 728 361 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2249 2756 4369 4743 4889 387
Size: 1064 138 660 507 1613 374 146 1280
Fragments arranged by size:
1613 1280 1064 660 507 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2517 3845 4442 5229 5497 1239
Size: 393 885 1328 597 787 268 1524
Fragments arranged by size:
1524 1328 885 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2021 2429 2021
Size: 408 5374
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 3076 4929 1678
Size: 1398 1853 2531
Fragments arranged by size:
2531 1853 1398
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2932 2964 4423 4455 4497
Size: 32 135 32 1360 32 1459 32 42
Cuts at: 4497 4529 1373
Size: 32 2626
Fragments arranged by size:
2626 1459 1360 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5782
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3960
Fragments arranged by size:
3960 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2385 2753 2898 3740 4099 4258
Size: 648 1077 142 368 145 842 359 159
Cuts at: 4258 518
Size: 2042
Fragments arranged by size:
2042 1077 842 648 368 359 159 145
142
MspA1I CmG'CkG
Cuts at: 1606 2545 2778 3198 3875 5023 5268 1606
Size: 939 233 420 677 1148 245 2120
Fragments arranged by size:
2120 1148 939 677 420 245 233
NcoI C'CATG_G
Cuts at: 513 1065 2004 2238 2356 2568 513
Size: 552 939 234 118 212 3727
Fragments arranged by size:
3727 939 552 234 212 118
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 3140 4685 1132
Size: 97 1911 1545 2229
Fragments arranged by size:
2229 1911 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2194 1108
Size: 1086 4696
PfoI T'CCnGG_A
Cuts at: 2075 2533 2075
Size: 458 5324
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2567 2599 3851 3883 5364 5396 2567
Size: 32 1252 32 1481 32 2953
Fragments arranged by size:
2953 1481 1252 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2021 2021
Size: 5782
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PstI C_TGCA'G
Cuts at: 830 3134 830
Size: 2304 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
SacII CC_GC'GG
Cuts at: 2779 2779
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SanDI GG'GwC_CC
Cuts at: 2021 2021
Size: 5782
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 2153 4030 5742 2153
Size: 1877 1712 2193
Fragments arranged by size:
2193 1877 1712
SfcI C'TryA_G
Cuts at: 826 1045 2619 3130 4265 4946 5137 826
Size: 219 1574 511 1135 681 191 1471
Fragments arranged by size:
1574 1471 1135 681 511 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5782
StyI C'CwwG_G
Cuts at: 513 1065 2004 2026 2238 2356 2568 3221 513
Size: 552 939 22 212 118 212 653 3074
Fragments arranged by size:
3074 939 653 552 212 212 118 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3809 3826 3979 4164 4609 325
Size: 1255 2229 17 153 185 445 1498
Fragments arranged by size:
2229 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 4028 5740
Size: 274 80 33 51 1443 174 1877 1712
Cuts at: 5740 96
Size: 138
Fragments arranged by size:
1877 1712 1443 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 3911 3911
Size: 5782
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI