Restriction map for
pFN21AB6527
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvaII AviII AvrII BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HindIII HpaI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 835 GACGTC GACGT^C
Aor51HI 119 AGCGCT AGC^GCT
Asp700I 756 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 101 TGGCCA TGG^CCA
BamHI 301 GGATCC G^GATCC
BbeI 47 GGCGCC GGCGC^C
BbrPI 724 CACGTG CAC^GTG
BpmI 1012 CTGGAG CTGGAG(16/14)
BsaAI 724 CACGTG [CT]AC^GT[GA]
BsgI 567 CTGCAC GTGCAG(16/14)
BsmAI 808 GAGAC GTCTC(1/5)
BsmI 420 GAATGC GAATGC(1/-1)
BspMI 925 ACCTGC ACCTGC(4/8)
BsrDI 164 GCAATG GCAATG(2/0)
BstXI 467 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 301 GGATCC [GA]^GATC[CT]
DpnII 302 GATC ^GATC
DsaI 77 CCACGG C^C[GA][CT]GG
Eco47III 119 AGCGCT AGC^GCT
EcoNI 885 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 810 GACTC G^A[ACGT]TC
KasI 47 GGCGCC G^GCGCC
MboI 302 GATC ^GATC
MflI 301 GGATCC [GA]^GATC[CT]
MluNI 101 TGGCCA TGG^CCA
MscI 101 TGGCCA TGG^CCA
MslI 451 CACAACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 47 GGCGCC GG^CGCC
NdeII 302 GATC ^GATC
PleI 810 GACTC GAGTC(4/5)
PmaCI 724 CACGTG CAC^GTG
PmlI 724 CACGTG CAC^GTG
PstI 849 CTGCAG CTGCA^G
SacI 619 GAGCTC GAGCT^C
Sau3AI 302 GATC ^GATC
ScaI 257 AGTACT AGT^ACT
SexAI 503 ACCTGGT A^CC[AT]GGT
SfaNI 432 GCATC GCATC(5/9)
SfcI 849 CTGCAG C^T[GA][CT]AG
SfoI 47 GGCGCC GGC^GCC
SmaI 360 CCCGGG CCC^GGG
StuI 893 AGGCCT AGG^CCT
XhoII 301 GGATCC [GA]^GATC[CT]
XmaCI 360 CCCGGG C^CCGGG
XmaI 360 CCCGGG C^CCGGG
XmnI 756 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AlwI 302 GATCC GGATC(4/5)
301 GGATC
AvaI 223 CCCGAG C^[CT]CG[GA]G
360 CCCGGG
BsmFI 833 GGGAC GGGAC(10/14)
655 GTCCC
BsoBI 223 CCCGAG C^[CT]CG[GA]G
360 CCCGGG
BsrI 353 ACTGG ACTGG(1/-1)
204 CCAGT
EarI 232 CTCTTC CTCTTC(1/4)
826 CTCTTC
HaeII 47 GGCGCC [GA]GCGC^[CT]
119 AGCGCT
HincII 157 GTCAAC GT[CT]^[GA]AC
700 GTCAAC
HindII 157 GTCAAC GT[CT]^[GA]AC
700 GTCAAC
Ksp632I 232 CTCTTC CTCTTC(1/4)
826 CTCTTC
MaeII 725 ACGT A^CGT
836 ACGT
SapI 231 GCTCTTC GCTCTTC(1/4)
825 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NgoMIV NlaIII NlaIV RsaI Sau96I
ScrFI StyI TaqI TseI TspRI
TthHB8I