(Circular) (Six-base) MAPSORT of: pFN21AB6527.seq Check: 8110 from: 1 to: 5800 pFN21AB6527 5800 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2939 2939 Size: 5800 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2844 3610 278 Size: 53 83 186 936 1308 766 2468 Fragments arranged by size: 2468 1308 936 766 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2636 2972 3044 3056 3863 5247 5515 1632 Size: 1004 336 72 12 807 1384 268 1917 Fragments arranged by size: 1917 1384 1004 807 336 268 72 12 AfeI AGC'GCT Cuts at: 2126 2126 Size: 5800 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 2460 1166 Size: 1294 4506 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2262 2294 2262 Size: 32 5768 AlwNI CAG_nnn'CTG Cuts at: 3543 5115 3543 Size: 1572 4228 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 2228 2365 3125 1870 Size: 73 285 137 760 4545 Fragments arranged by size: 4545 760 285 137 73 BamHI G'GATC_C Cuts at: 2306 3130 3497 2306 Size: 824 367 4609 Fragments arranged by size: 4609 824 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2052 2361 2502 2775 3121 Size: 325 217 627 265 309 141 273 346 Cuts at: 3121 4576 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 346 325 309 273 265 217 141 BanII G_rGCy'C Cuts at: 1391 2072 2238 2628 3119 1391 Size: 681 166 390 491 4072 Fragments arranged by size: 4072 681 491 390 166 BbeI G_GCGC'C Cuts at: 2056 2056 Size: 5800 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4829 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4411 136 Size: 107 122 71 631 605 337 2402 1525 Fragments arranged by size: 2402 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 2209 2778 3861 5017 1790 Size: 419 569 1083 1156 2573 Fragments arranged by size: 2573 1156 1083 569 419 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2203 3531 4489 Size: 416 53 83 399 24 786 1328 958 Cuts at: 4489 442 Size: 1753 Fragments arranged by size: 1753 1328 958 786 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3073 3073 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3038 4460 1239 Size: 1799 1422 2579 Fragments arranged by size: 2579 1799 1422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3925 4790 5088 3925 Size: 865 298 4637 Fragments arranged by size: 4637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4463 882 Size: 617 2964 2219 Fragments arranged by size: 2964 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2731 3591 493 Size: 711 1527 860 2702 Fragments arranged by size: 2702 1527 860 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2053 2100 2547 2658 Size: 53 83 186 936 520 47 447 111 Cuts at: 2658 2841 3607 3989 4624 275 Size: 183 766 382 635 1451 Fragments arranged by size: 1451 936 766 635 520 447 382 186 183 111 83 53 47 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5575 Fragments arranged by size: 5575 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5800 BseYI C'CCAG_C Cuts at: 2017 2713 3069 5003 5499 2017 Size: 696 356 1934 496 2318 Fragments arranged by size: 2318 1934 696 496 356 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2557 1163 Size: 573 821 4406 Fragments arranged by size: 4406 821 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 4011 5039 1540 Size: 275 186 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 2628 2739 3861 3946 5017 2193 Size: 435 111 1122 85 1071 2976 Fragments arranged by size: 2976 1122 1071 435 111 85 BsmI GAATG_Cn' Cuts at: 2431 3311 3404 2431 Size: 880 93 4827 Fragments arranged by size: 4827 880 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2193 2209 2238 2628 2739 2778 Size: 399 282 121 16 29 390 111 39 Cuts at: 2778 3119 3861 3946 5017 1391 Size: 341 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 341 282 121 111 85 39 29 16 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2939 3155 844 Size: 2095 216 3489 Fragments arranged by size: 3489 2095 216 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2176 4295 4469 65 Size: 1183 928 2119 174 1396 Fragments arranged by size: 2119 1396 1183 928 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 2061 2429 2445 2682 2778 4444 Size: 110 236 15 368 16 237 96 1666 Cuts at: 4444 4631 1700 Size: 187 2869 Fragments arranged by size: 2869 1666 368 237 236 187 110 96 16 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 3860 4872 3860 Size: 1012 4788 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 2479 2479 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2306 3130 3497 3882 3899 5351 5437 5449 Size: 334 824 367 385 17 1452 86 12 Cuts at: 5449 5795 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 824 385 367 346 334 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2082 513 Size: 552 939 78 4231 Fragments arranged by size: 4231 939 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2889 487 Size: 683 56 1663 3398 Fragments arranged by size: 3398 1663 683 56 BtsI GCAGTG_nn' Cuts at: 2191 2729 3316 4110 4130 2191 Size: 538 587 794 20 3861 Fragments arranged by size: 3861 794 587 538 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2106 2329 Size: 54 1425 50 275 18 198 78 223 Cuts at: 2329 3247 4136 5765 8 Size: 918 889 1629 43 Fragments arranged by size: 1629 1425 918 889 275 223 198 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2243 2837 3730 1353 Size: 890 594 893 3423 Fragments arranged by size: 3423 893 890 594 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2626 2626 Size: 5800 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2636 2972 3038 3044 3056 3863 4460 Size: 393 1004 336 66 6 12 807 597 Cuts at: 4460 5247 5515 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1004 807 787 597 393 336 268 66 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 2894 2894 Size: 5800 EcoO109I rG'GnC_Cy Cuts at: 2476 2673 2797 2476 Size: 197 124 5479 Fragments arranged by size: 5479 197 124 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 2056 2128 4947 1678 Size: 378 72 2819 2531 Fragments arranged by size: 2819 2531 378 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2867 2899 3077 3109 4441 Size: 32 135 32 1295 32 178 32 1332 Cuts at: 4441 4473 4515 4547 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1332 1295 178 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2164 2707 3144 3390 669 Size: 988 281 226 543 437 246 3079 Fragments arranged by size: 3079 988 543 437 281 246 226 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KasI G'GCGC_C Cuts at: 2052 2052 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2076 3027 4914 5098 1305 Size: 771 951 1887 184 2007 Fragments arranged by size: 2007 1887 951 771 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 10 Size: 54 1425 343 276 3702 Fragments arranged by size: 3702 1425 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2460 3758 4117 4276 518 Size: 648 1294 1298 359 159 2042 Fragments arranged by size: 2042 1298 1294 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2379 2500 3016 3216 3893 5041 5286 1606 Size: 773 121 516 200 677 1148 245 2120 Fragments arranged by size: 2120 1148 773 677 516 245 200 121 NaeI GCC'GGC Cuts at: 2431 2447 2684 2431 Size: 16 237 5547 Fragments arranged by size: 5547 237 16 NarI GG'CG_CC Cuts at: 2053 2053 Size: 5800 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NgoMIV G'CCGG_C Cuts at: 2429 2445 2682 2429 Size: 16 237 5547 Fragments arranged by size: 5547 237 16 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 3158 4703 1132 Size: 97 1929 1545 2229 Fragments arranged by size: 2229 1929 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PmlI CAC'GTG Cuts at: 2731 2731 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PstI C_TGCA'G Cuts at: 830 2858 3152 830 Size: 2028 294 3478 Fragments arranged by size: 3478 2028 294 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 SacI G_AGCT'C Cuts at: 2628 2628 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SapI GCTCTTCn'nnn_ Cuts at: 2243 2837 2243 Size: 594 5206 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 2264 4048 5760 2264 Size: 1784 1712 2304 Fragments arranged by size: 2304 1784 1712 SexAI A'CCwGG_T Cuts at: 2508 2508 Size: 5800 SfcI C'TryA_G Cuts at: 826 1045 2854 3148 4283 4964 5155 826 Size: 219 1809 294 1135 681 191 1471 Fragments arranged by size: 1809 1471 1135 681 294 219 191 SfoI GGC'GCC Cuts at: 2054 2054 Size: 5800 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 2367 3127 1872 Size: 495 760 4545 Fragments arranged by size: 4545 760 495 SmlI C'TyrA_G Cuts at: 820 1017 1943 3904 4805 5067 820 Size: 197 926 1961 901 262 1553 Fragments arranged by size: 1961 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2900 1517 Size: 1383 4417 StyI C'CwwG_G Cuts at: 513 1065 2004 2103 2472 3075 3239 513 Size: 552 939 99 369 603 164 3074 Fragments arranged by size: 3074 939 603 552 369 164 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2009 2591 3827 3844 3997 4182 4627 Size: 1255 429 582 1236 17 153 185 445 Cuts at: 4627 325 Size: 1498 Fragments arranged by size: 1498 1255 1236 582 445 429 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2262 2487 4046 Size: 274 80 33 51 1443 285 225 1559 Cuts at: 4046 5758 96 Size: 1712 138 Fragments arranged by size: 1712 1559 1443 285 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5760 5792 5760 Size: 32 5768 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 2365 3125 1870 Size: 495 760 4545 Fragments arranged by size: 4545 760 495 XmnI GAAnn'nnTTC Cuts at: 2765 3929 2765 Size: 1164 4636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2842 3608 276 Size: 53 83 186 936 1308 766 2468 Fragments arranged by size: 2468 1308 936 766 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SrfI SwaI XcmI